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Effect of bax, bcl-2 and bcl-xL on regulating apoptosis in tissues of normal liver and hepatocellular carcinoma

2002; Baishideng Publishing Group; Volume: 8; Issue: 6 Linguagem: Inglês

10.3748/wjg.v8.i6.1059

2219-2840

Xiaozhong Guo,

Hepatitis B Virus Studies

AIM:To investigate the expression of bax, bcl-2 and bcl-xL mRNA in the tissues of normal liver and hepatocellular carcinoma (HCC), and analyze the relationship between the expression of bax, bcl-2 and bcl-xL mRNA and clinical parameters of HCC patients. METHODS:The expression of bax, bcl-2 and bcl-xL mRNA of normal liver and HCC was measured by Northern blot.Statistical analyses were made by t test and correlation analysis. RESULTS:A very low mRNA level was indicated at bax, bcl-2 and bcl-xL in the HCC tissues in contrast to the tissues of normal liver by Northern blot analysis.The analyses of mRNA level revealed that HCC tissues exhibited a mean 7.6-fold decrease in bax, 4.2-fold in bcl-2 and 3.5-fold in bcl-xL in comparison with normal control tissues, respectively.Positive correlation was found between bax and bcl-xL (r=0.7061,P<0.01).There was no significance between the mRNA expression of these three genes and age, gender, tumor differentiation and tumor stage of HCC patients . CONCLUSION:The results are consistent with the fact that apoptosis rarely occurs in normal livers but increases in HCC, indicating that bcl-2 and bcl-xL may play a very important role in regulating the apoptosis of normal liver and HCC.