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A Mutant of Escherichia coli That Requires High Concentrations of Biotin

1972; National Academy of Sciences; Volume: 69; Issue: 3 Linguagem: Inglês

10.1073/pnas.69.3.676

1091-6490

Allan Campbell, Alice del Campillo-Campbell, Robin Chang,

Chemical Synthesis and Analysis

A recessive mutation ( bir ) of E. coli , at 77 min on the standard map, destroys ability of the bacteria to take up biotin or to grow except at high concentrations of biotin. In a biotin prototroph, the bir mutation causes overproduction and excretion of biotin, and derepression of at least one biosynthetic gene ( bioD ). Growth of the bir mutant is inhibited by avidin or high concentrations of dethiobiotin. Both effects are reversed by biotin.