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Sortase-Catalyzed Peptide−Glycosylphosphatidylinositol Analogue Ligation

2009; American Chemical Society; Volume: 131; Issue: 29 Linguagem: Inglês

10.1021/ja903231v

1943-2984

Xueqing Guo, Qianli Wang, Benjamin M. Swarts, Zhongwu Guo,

Botanical Research and Chemistry

It is demonstrated that sortase A (SrtA) can catalyze efficient coupling of peptides to GPI analogues with a glycine residue attached to the phosphoethanolamine moiety at the nonreducing end to form GPI-linked peptides. This represents the first chemoenzymatic synthesis of GPI−peptide conjugates and is a proof-of-concept for the potential application of SrtA to the synthesis of more complex GPI-anchored peptides/glycopeptides and GPI-anchored proteins/glycoproteins.