Cloning, expression and antimicrobial activity of an antimicrobial peptide, epinecidin-1, from the orange-spotted grouper, Epinephelus coioides
2005; Elsevier BV; Volume: 253; Issue: 1-4 Linguagem: Inglês
10.1016/j.aquaculture.2005.10.002
ISSN1873-5622
AutoresZhi-Xin Yin, Wei He, Weijian Chen, Jiang-Hui Yan, Junning Yang, Siu‐Ming Chan, Jianguo He,
Tópico(s)Invertebrate Immune Response Mechanisms
ResumoOutbreaks of infectious diseases have caused huge losses in the fish culture industry. The production of antimicrobial peptides has been identified as a major defense mechanism against infections. A cDNA encoding an antimicrobial peptide was isolated from the leukocyte cDNA library of orange-spotted grouper, Epinephelus coioides. The predicted 67-amino acid prepropeptide, named epinecidin-1, consists of three domains: a signal peptide of 22 amino acids, a mature peptide of 25 amino acids, and a carboxy-terminal prodomain of 20 amino acids. The epinecidin-1 gene consisted of three introns and four exons. A TATA box and several consensus-binding motifs for transcription factors were found in the proximal region 5′ to the transcription initiation site. A synthetic, amidated mature peptide of epinecidin-1 exhibited high antimicrobial activity against Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio vulnificus, Pasturella multocida, Morganella morganii, Aeromonas sobrio, Aeromonas hydrophila, Flavobacterium meningosepticum and Escherichia coli DH5α (minimal bactericidal concentration (MBC) < 5 μM). Most of these bacteria are known pathogens in aquaculture. Some fungi, such as Candida albicans and Microsporosis sanis, were also sensitive to this synthetic peptide (MBC < 20 μM). In conclusion, epinecidin-1 may be effective in the treatment or prevention of bacterial infections in aquaculture, and it may be a candidate gene for the generation of transgenic fish to improve disease resistance in aquaculture.
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