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Preparation of chitosan particles suitable for enzyme immobilization

2007; Elsevier BV; Volume: 70; Issue: 6 Linguagem: Inglês

10.1016/j.jprot.2007.11.005

1872-857X

Emese Biró, Ágnes Sz. Németh, Csaba Sisak, Tivadar Feczkó, János Gyenis,

Proteins in Food Systems

Macro-, micro- and nanosized chitosan particles suitable as immobilization carriers were prepared by precipitation, emulsion cross-linking and ionic gelation methods, respectively. Effects of particle preparation parameters on particle size were investigated. Activities of β-galactosidase covalently attached to differently sized particles have been evaluated and compared. The highest activity was shown by the biocatalyst immobilized on nanoparticles obtained by means of the ionotropic gelation method with sodium sulphate as gelation agent. β-Galactosidase fixed on macro- and microspheres exhibited excellent storage stability in aqueous solution, with no more than 5% loss of activity after 3 weeks storage at 4 °C and pH 7.0.