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Growth and alkaloid production in callus tissues of Rauwolfia serpentina.

1979; Oxford University Press; Volume: 43; Issue: 11 Linguagem: Inglês

10.1271/bbb1961.43.2297

1881-1280

Shinsuke Ohta, Michihiko Yatazawa,

Plant tissue culture and regeneration

Callus tissues were induced from stem and root segments of Rauwolfa serpentina. Growth and alkaloid production of the callus tissues were examined under various culture conditions. The growth was strikingly promoted in the presence of 2, 4-D (0.5_??_1ppm), kinetin (0.2_??_0.5ppm) and yeast extract (0.1_??_0.2%). At favourable conditions, the growth value in 4 weeks' culture was ca. 40 (F. W.), and ca. 25 (D. W.) for stem callus tissues, and ca. 15 (F. W.), and ca. 8 (D. W.) for root callus tissues. Stem and root callus tissues produced ajmaline and some other unidentified Rauwolfia alkaloids. The ajmaline content in root callus tissues was 10_??_20mg% and in stem callus tissues was 1_??_10mg%, The ajmaline production was strikingly reduced when 2, 4-D concentration increased, or kinetin was omitted in the culture medium. Phytosterols including stigmasterol, β-sitosterol or cholesterol were also produced.