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Amplification and rearrangement of a prokaryotic metallothionein locus smt in Synechococcus PCC 6301 selected for tolerance to cadmium

1992; Royal Society; Volume: 248; Issue: 1323 Linguagem: Inglês

10.1098/rspb.1992.0072

1471-2954

Amit Gupta, Brian A. Whitton, Andrew P. Morby, James W. Huckle, Nigel J. Robinson,

Environmental Toxicology and Ecotoxicology

Restricted accessMoreSectionsView PDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmail Cite this article Gupta Amit , Whitton Brian A. , Morby Andrew P. , Huckle James W. and Robinson Nigel J. 1992Amplification and rearrangement of a prokaryotic metallothionein locus smt in Synechococcus PCC 6301 selected for tolerance to cadmiumProc. R. Soc. Lond. B.248273–281http://doi.org/10.1098/rspb.1992.0072SectionRestricted accessArticleAmplification and rearrangement of a prokaryotic metallothionein locus smt in Synechococcus PCC 6301 selected for tolerance to cadmium Amit Gupta Google Scholar Find this author on PubMed Search for more papers by this author , Brian A. Whitton Google Scholar Find this author on PubMed Search for more papers by this author , Andrew P. Morby Google Scholar Find this author on PubMed Search for more papers by this author , James W. Huckle Google Scholar Find this author on PubMed Search for more papers by this author and Nigel J. Robinson Google Scholar Find this author on PubMed Search for more papers by this author Amit Gupta Google Scholar Find this author on PubMed , Brian A. Whitton Google Scholar Find this author on PubMed , Andrew P. Morby Google Scholar Find this author on PubMed , James W. Huckle Google Scholar Find this author on PubMed and Nigel J. Robinson Google Scholar Find this author on PubMed Published:22 June 1992https://doi.org/10.1098/rspb.1992.0072AbstractMetal-tolerant cyanobacteria have been isolated from metal-polluted aquatic environments and also selected in culture, but no genes which confer metal tolerance have been described. To investigate the possibility that amplification of a prokaryotic metallothionein gene (smtA), or rearrangement o f the smt locus, could be involved in the development of Cd tolerance in Synechococcus PCC 6301, Cd-tolerant lines were selected by stepwise adaptation of a Synechococcus culture. An increase in smtA gene copy number and the appearance of unique additional smtA restriction fragments (both larger and smaller) were detected in these tolerant lines (tolerant to 0.8 μM Cd, 1.3 μM Cd and 1.7 μM Cd). Stepwise adaptation was repeated by using a culture of Synechococcus PCC 6301 inoculated from a single plated colony to obtain four new lines (tolerant to 1.4 μM Cd, 1.8 μM Cd, 2.6 μM Cd and 3.2 μM Cd). Amplification of the smtA gene and development of unique smtA restriction fragments (larger and smaller) were once again detected in these tolerant lines. Amplification and rearrangement o f the smt locus were only detected in the seven Cd-tolerant lines, with no evidence of amplification or rearrangement in the non-tolerant lines from which they were derived. As a control, another gene, psaE, was also monitored in these cell lines. There was no evidence of amplification or rearrangement of psaE in the non-tolerant or any of the Cd-tolerant lines.FootnotesThis text was harvested from a scanned image of the original document using optical character recognition (OCR) software. As such, it may contain errors. Please contact the Royal Society if you find an error you would like to see corrected. Mathematical notations produced through Infty OCR. 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