Erum-induced changes in electrophoretic mobility of creatine phosphokinase isoenzymes?
1977; Elsevier BV; Volume: 79; Issue: 1 Linguagem: Inglês
10.1016/0009-8981(77)90484-3
ISSN1873-3492
AutoresPéter Bayer, Th. Gergely, F Gabl, Éric Deutsch,
Tópico(s)Muscle Physiology and Disorders
ResumoBB isoenzyme of creatine phosphokinase (CK-BB) obtained from human brain-extract changes its electrophoretic mobility after incubation in human serum at 37 degrees C. No change of electrophoretic mobility of CK-BB is observed after incubation in isotonic saline. We have shown by means of immunoprecipitation with specific antibodies that the structure of CK-BB is not changed. These findings are supported by other authors and make the diagnostic value of electrophoretic separation of CK isoenzymes doubtful as after a 3-h incubation CK-BB migrates similarly to CK-BB and consequently may be misinterpreted.
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