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Fluorogenic probes for live-cell imaging of the cytoskeleton

2014; Nature Portfolio; Volume: 11; Issue: 7 Linguagem: Inglês

10.1038/nmeth.2972

1548-7105

Gražvydas Lukinavičius, Luc Reymond, Elisa D’Este, Anastasiya Masharina, Fabian Göttfert, Haisen Ta, Angelika Güther, Mathias Fournier, Stefano Rizzo, Herbert Waldmann, Claudia Blaukopf, Christoph Sommer, Daniel W. Gerlich, Hans‐Dieter Arndt, Stefan W. Hell, Kai Johnsson,

Advanced Electron Microscopy Techniques and Applications

Far-red fluorogenic probes for live-cell imaging of either actin or tubulin are described and used for super-resolution microscopy of various structures in a variety of cell types. We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.