Biochemistry and function of the DISC
2001; Elsevier BV; Volume: 26; Issue: 7 Linguagem: Inglês
10.1016/s0968-0004(01)01895-3
ISSN1362-4326
AutoresHenning Walczak, Martin R. Sprick,
Tópico(s)Drug Transport and Resistance Mechanisms
ResumoThe DISC (death-inducing signalling complex) is one of two apoptosis-initiating protein complexes discovered to date. Whereas the so-called apoptosome, the second of these two complexes, is cytoplasmic, the DISC actually assembles at the plasma membrane. The name for this protein complex was coined in 1995 when Kischkel et al. used the apoptosis-inducing anti-APO-1 antibody to crosslink the CD95 death receptor (also known as APO-1 or Fas) on intact cells. They analysed the resulting protein complex by immunoprecipitation with the stimulating anti-APO-1 antibody followed by 2D gel electrophoresis. This led to the definition of spots on the 2D gels that represented proteins (i.e. components of the DISC) associated with the stimulated, but not the unstimulated, receptor.
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