High-performance liquid chromatography and capillary gel electrophoresis as applied to antisense DNA
1993; Elsevier BV; Volume: 638; Issue: 2 Linguagem: Inglês
10.1016/0021-9673(93)83441-t
ISSN1873-3778
AutoresAharon S. Cohen, Maria Vilenchik, Judith L. Dudley, M W Gemborys, A. J. Bourque,
Tópico(s)DNA and Nucleic Acid Chemistry
ResumoReversed-phase and ion-exchange HPLC are compared with both slab gel and capillary gel electrophoresis for the separation of antisense phosphorothioate oligomers. The chromatographic separations were found to be markedly inferior to the electrophoretic separations, especially for oligomers greater than 20 bases in length. The potential of gel high-performance capillary electrophoresis for the analysis of phosphorothioate analogues is shown.
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