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The Contribution of Arabidopsis Homologs of L -Gulono-1,4-lactone Oxidase to the Biosynthesis of Ascorbic Acid

2010; Oxford University Press; Volume: 74; Issue: 7 Linguagem: Inglês

10.1271/bbb.100157

1347-6947

Takanori Maruta, Yaka Ichikawa, Takahiro Mieda, Toru Takeda, Masahiro Tamoi, Yukinori Yabuta, Takahiro Ishikawa, Shigeru Shigeoka,

Microbial bioremediation and biosurfactants

To clarify the involvement of seven Arabidopsis homologs of rat L-gulono-1,4-lactone (L-GulL) oxidase, AtGulLOs, in the biosynthesis of L-ascorbic acid (AsA), transgenic tobacco cells overexpressing the various AtGulLOs were generated. Under treatment with L-GulL, the levels of total AsA in three transgenic tobacco cell lines, overexpressing AtGulLO2, 3, or 5, were significantly increased as compared with those in control cells.