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Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution

2010; National Academy of Sciences; Volume: 107; Issue: 46 Linguagem: Inglês

10.1073/pnas.1009999107

1091-6490

Jean‐Paul Armache, A. Jarasch, Andreas M. Anger, Elizabeth Villa, Thomas Becker, Shashi Bhushan, Fabrice Jossinet, Michael Habeck, G. Dindar, S. Franckenberg, V. Marquez, Thorsten Mielke, Michael Thomm, Otto Berninghausen, Birgitta Beatrix, Johannes Söding, Éric Westhof, Daniel N. Wilson, Roland Beckmann,

Genomics and Phylogenetic Studies

Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant ( Triticum aestivum ) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES–ES and r-protein–ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.