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Metacortandracin and 9-Alpha-Fluoro Hydrocortisone Acetate in Rheumatic Diseases

1955; BMJ; Volume: 14; Issue: 3 Linguagem: Inglês

10.1136/ard.14.3.251

1468-2060

L. F. Villa, C. B. Ballabio, Giovanni Battista La Sala,

Heat shock proteins research

Abstract Gap junctions have well-established roles in cell-cell communication by way of forming permeable intercellular channels. Less is understood about their internalization, which forms double membrane vesicles containing cytosol and membranes from another cell, called connexosomes or annular gap junctions. Here, we systematically studied the fate of connexosomes in intact ovarian follicles. High pressure frozen, serial sectioned tissue was immunogold labeled for Connexin 43. Within a volume of electron micrographs, every labeled structure was categorized and counted. Surface area measurements indicate that large connexosomes undergo fission. Subsequent modifications are separation of inner and outer membranes, loss of Cx43 from the outer membrane, and outward budding of the modified membranes. We also documented several clear examples of organelle transfer from one cell to another by gap junction internalization. We discuss how connexosome formation and processing may be a novel means for gap junctions to mediate cell-cell communication.