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HeLa-Like Marker Chromosomes and Type-A Variant Glucose-6-phosphate Dehydrogenase Isoenzyme in Human Cell Cultures Producing Mason-Pfizer Monkey Virus-Like Particles2

1974; Oxford University Press; Volume: 53; Issue: 3 Linguagem: Inglês

10.1093/jnci/53.3.751

1460-2105

Walter A. Nelson‐Rees, V. M. Zhdanov, Paula K. Hawthorne, Robert R. Flandermeyer,

Herpesvirus Infections and Treatments

A virus similar to the Mason-Pfizer monkey virus (M-PMV) has been discovered in certain human cell lines by workers in the United States. It was extensively studied in cell line AO. Other laboratories have also discovered this virus or similar viruses in certain human cells. Since this virus had been isolated in 1970 from only a rhesus monkey mammary adenocarcinoma, it was deemed necessary to study these cells for species origin and possible donor characteristics. We examined the chromosomes and glucose-6-phosphate dehydrogenase (G6PD) mobility patterns of AO and 5 other cell lines [H.EP.#2 (clone), J96, DAPT, T-9, and CaOV]. Conventional Giemsa staining revealed hypotriploidy for human chromosomes in all cells of all lines. Quinacrine fluorescence indicated the absence from all cells of a Y chromosome either alone or in translocation with another chromosome. A trypsinGiemsa technique for chromosome banding revealed 1) some marker chromosomes unique to each cell line and 2) several marker chromosomes common to all cell lines, as well as to our culture of HeLa cells and to other cultures of HeLa cells reported in the literature. G6PD mobility for all lines was of the type-A variant also characteristic of HeLa cells. We concluded that, while certain chromosomal differences existed between these 6 cell lines and HeLa cell cultures, their possession, in common with HeLa, of an isoenzyme mobility pattern for G6PD and of unique marker chromosomes clearly established their derivation from the HeLa cell line.