Portal de Periódicos da CAPES

RNA Sequencing of Single Human Islet Cells Reveals Type 2 Diabetes Genes

2016; Cell Press; Volume: 24; Issue: 4 Linguagem: Inglês

10.1016/j.cmet.2016.08.018

1932-7420

Yurong Xin, Jinrang Kim, Haruka Okamoto, Min Ni, Yi Wei, Christina Adler, Andrew Murphy, George D. Yancopoulos, Calvin Lin, Jesper Gromada,

Diabetes and associated disorders

Pancreatic islet cells are critical for maintaining normal blood glucose levels, and their malfunction underlies diabetes development and progression. We used single-cell RNA sequencing to determine the transcriptomes of 1,492 human pancreatic α, β, δ, and PP cells from non-diabetic and type 2 diabetes organ donors. We identified cell-type-specific genes and pathways as well as 245 genes with disturbed expression in type 2 diabetes. Importantly, 92% of the genes have not previously been associated with islet cell function or growth. Comparison of gene profiles in mouse and human α and β cells revealed species-specific expression. All data are available for online browsing and download and will hopefully serve as a resource for the islet research community.