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Immunomodulating role of IL-10-producing B cells in Leishmania amazonensis infection

2018; Elsevier BV; Volume: 334; Linguagem: Inglês

10.1016/j.cellimm.2018.08.014

1090-2163

Luan Firmino-Cruz, Tadeu Diniz Ramos, Alessandra Marcia da Fonseca-Martins, Diogo Maciel-Oliveira, Gabriel Oliveira-Silva, Juliana Elena Silveira Pratti, Cecília B. Cavazzoni, Suzana Passos Chaves, Daniel Claúdio Oliveira Gomes, Alexandre Morrot, Leonardo Freire‐de‐Lima, André M. Vale, Célio Geraldo Freire-de-Lima, Débora Decoté‐Ricardo, Herbert Leonel de Matos Guedes,

Autoimmune and Inflammatory Disorders Research

This work aims to study the immunomodulation of B lymphocytes during L. amazonensis infection. We demonstrated in this study that follicular B cells from draining lymph nodes of infected wild type BALB/c mice are the major source of IL-10 during infection. We infected BALB/Xid mice that developed smaller lesions in comparison with the control, but the parasite load obtained from the infected tissues was similar in both groups. We observed a reduction in the number of follicular B cells from BALB/Xid mice in relation to WT mice and, consequently, lower levels of IgM, IgG, IgG1, IgG2a and IgG2b in the serum of BALB/Xid when compared with wild type mice. BALB/Xid mice also presented lower levels of IL-10 in the infected footpad, draining lymph nodes and in the spleen when compared with WT infected tissues. We did not detect differences in the number of IL-10 producing CD4+ and CD8+ T cells between WT and BALB/Xid mice; however, a strong reduction of IL-10 producing follicular B cells was noted in BALB/Xid mice. When analyzed together, our data indicate that B cells are related with lesion pathogenesis through the production of antibodies and IL-10.