... José A. Moura-Neto, Lucio R. Requião-Moura, Álvaro Pacheco-Silva Preemptive kidney transplantation: why, when, and how?, ... José A. Moura-Neto, Lucio R. Requião-Moura, Álvaro Pacheco-Silva Transplante renal preemptivo: por que, quando e ...
Tópico(s): Organ Donation and Transplantation
1980 - University of Chicago Press | Ethics
Inês Baldeiras, Maria Helena Ribeiro, Paula Pacheco, Álvaro Machado, Isabel Santana, Luı́s Cunha, Catarina R. Oliveira,
The clinical diagnosis of sporadic Creutzfeldt-Jakob disease (sCJD) is difficult, and reliable markers are highly desired. In this work we assess the value of several cerebrospinal fluid (CSF) markers for sCJD diagnosis. Within the framework of the Portuguese Epidemiological Surveillance Program for Human Prion Diseases, CSF samples from 71 patients with clinically suspected sCJD, 30 definite sCJD and 41 non-CJD patients, were analysed for the presence of 14-3-3 protein. CSF levels of tau (t-tau), ...
Tópico(s): Neurological diseases and metabolism
2009 - Springer Science+Business Media | Journal of Neurology
R. Zafra, Alejandro Pérez-Écija, Leandro Buffoni, Isabel Pacheco, Álvaro Martínez‐Moreno, E. James LaCourse, Samïrah Perally, Peter M. Brophy, J. Pérez,
Changes and local immune response were evaluated in the peritoneal cell populations, duodenal lamina propria and liver from goats immunized with recombinant glutathione transferase sigma class (rFhGST-S1) during early stages of infection with Fasciola hepatica. Group 1 (n = 7) was unimmunized and uninfected; group 2 (n = 10) was immunized with adjuvant Quil A and infected; group 3 (n = 10) was immunised with rFhGST-S1 and infected. Three goats from each group were killed at 7–9 days post-infection (dpi) to ...
Tópico(s): Parasites and Host Interactions
2012 - Elsevier BV | Research in Veterinary Science
Mariluz Parga, Maite Pons, Sandra Andraka, Liliana Rendón, Takahisa Mituhasi, Martín Hall, Lucas Pacheco, Álvaro Segura, Michael Osmond, Nick Vogel,
Tópico(s): Marine animal studies overview
2015 - Elsevier BV | Fisheries Research
Produção Nacional
Revisado por pares
Natália Moreira Nunes, Ana Flávia Coelho Pacheco, Álvaro Javier Patiño‐Agudelo, Luís Henrique Mendes da Silva, Maximiliano Soares Pinto, Maria do Carmo Hespanhol da Silva, Ana Clarissa dos Santos Pires,
Cinnamic acid (CA) and methyl cinnamate (MC) have attracted interest of researchers because of their broad therapeutic functions. Here, we investigated the interaction of CA and MC with bovine serum albumin (BSA) at pH 3.5, 5.0, and 7.4 using fluorescence spectroscopy, differential scanning nanocalorimetry, and measurements of interfacial tension, size, and zeta potential. BSA formed a complex with the ligands with stoichiometry of approximately 1.0. At pH 7.4, CA-BSA complex formation was entropically ...
Tópico(s): Photochemistry and Electron Transfer Studies
2017 - Elsevier BV | Food Chemistry
Teobaldo E. Torres, Enio Lima, M. Pilar Calatayud, Beatriz Sanz, Alfonso Ibarra, Rodrigo Fernández‐Pacheco, Álvaro Mayoral, C. Marquina, M. R. Ibarra, Gerardo F. Goya,
Abstract The Linear Response Theory (LRT) is a widely accepted framework to analyze the power absorption of magnetic nanoparticles for magnetic fluid hyperthermia. Its validity is restricted to low applied fields and/or to highly anisotropic magnetic nanoparticles. Here, we present a systematic experimental analysis and numerical calculations of the specific power absorption for highly anisotropic cobalt ferrite (CoFe 2 O 4 ) magnetic nanoparticles with different average sizes and in different viscous ...
Tópico(s): Iron oxide chemistry and applications
2019 - Nature Portfolio | Scientific Reports
Gilberto Gutiérrez García, Gabriela Galicia García, Jessica Zalapa Soto, Andrea Medina, Mariana Rotzinger-Rodríguez, Gustavo Antonio Casas Aguilar, Cynthia Paola López Pacheco, Álvaro Aguayo, Montserrat Aguilar-Hernández,
Extracellular vesicles (EV) have attracted much attention as potential biomarkers due to their protein, RNA and other nucleic acid content. The most common method used for EV isolation is differential ultracentrifugation (DU), however given the DU technical difficulties, other more practical methods have surged, such as membrane-affinity column commercial kits. Here, we assessed one commercial kit in terms of EV recovery and EV-derived RNA yield and compared it with a DU protocol. Our data shows that ...
Tópico(s): RNA Research and Splicing
2020 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
Eduardo Santos da Silva, Lorenz Aglas, Carina S. Pinheiro, Emília Maria Medeiros de Andrade Belitardo, Elisânia Fontes Silveira, Sara Huber, Rogério Tanan Torres, Michael Wallner, Peter Briza, Peter Lackner, Josef Laimer, Luis G. C. Pacheco, Álvaro A. Cruz, Neuza Maria Alcântara‐Neves, Fátima Ferreira,
Abstract Introduction Allergen‐specific immunotherapy (AIT) represents a curative approach for treating allergies. In the tropical and subtropical regions of the world, Blomia tropicalis (Blo t 5 and Blo t 21) is the likely dominant source of indoor allergens. Aim To generate a hypoallergenic Blo t 5/Blo t 21 hybrid molecule that can treat allergies caused by B tropicalis . Methods Using in silico design of B tropicalis hybrid proteins, we chose two hybrid proteins for heterologous expression. Wild‐ ...
Tópico(s): Asthma and respiratory diseases
2020 - Wiley | Clinical & Experimental Allergy
Raúl Izaguirre-Ávila, A. De la Peñia-Díaz, Fernando Barinagarrementería-Aldatz, H. Gonzáilez-Pacheco, Álvaro E. Ramírez-Gutiérrez, José Luis Ruíz‐Sandoval, A. Quiroz-Martínez, Carlos Cantú‐Brito,
Acetylsalicylic acid inhibits thromboxane A2 production and reduces the risk of vascular occlusive events by 20% to 25%. Ticlopidine inhibits ADP-dependent platelet aggregation and reduces the same risk by 30% to 35%, but produces some adverse effects. Clopidogrel is a ticlopidin-related antiplatelet drug, with the same mechanism of action; it reduces the expression of the glycoprotein Ilb/Illa, the fibrinogen receptor on the platelet surface. Clopidogrel has the same clinical efficacy of ticlopidin ...
Tópico(s): Lipoproteins and Cardiovascular Health
2002 - SAGE Publishing | Clinical and Applied Thrombosis/Hemostasis
Alfredo Tagarro, Francisco José Sanz-Santaeufemia, Carlos Grasa, Elena Cobos, Julia Yebra, José Antonio Alonso‐Cadenas, Fernando Baquero‐Artigao, Juan Miguel Mesa-Guzmán, Beatriz Pérez-Seoane, Cristina Calvo, María Luisa Herreros, Cristina Epalza, Susana Melendo, Sara Domínguez‐Rodríguez, Paula Vidal, Mónica Pacheco, Álvaro Ballesteros, Maria de Lourdes Amaral Bernardino, Sara Villanueva-Medina, Paula Rodríguez-Molino, Sandra Miragaya Castro, Jacques G. Rivière, Rosa Garcés, Begoña Santiago, Victòria Fumadó, María Urretavizcaya-Martínez, María Luz García‐García, María Penín, Fernando Cava, Elena Sáez, María Isabel Iglesias‐Bouzas, Blanca Herrero, Teresa de Jesús Reinoso, Cinta Moraleda,
ObjectivesTo determine the time to reverse transcription-polymerase chain reaction (RT-PCR) negativity after the first positive RT-PCR test, factors associated with longer time to RT-PCR negativity, proportion of children seroconverting after proven severe acute respiratory syndrome coronavirus 2 infection, and factors associated with the lack of seroconversion.Study designThe Epidemiological Study of Coronavirus in Children of the Spanish Society of Pediatrics is a multicenter study conducted in ...
Tópico(s): Viral gastroenteritis research and epidemiology
2021 - Elsevier BV | The Journal of Pediatrics
Produção Nacional
Revisado por pares
Patrícia Semedo, Matheus Corrêa-Costa, Marcos Antônio Cenedeze, Denise Maria Avancini Costa Malheiros, Marlene Antônia dos Reis, Maria Heloísa Massola Shimizu, Antônio Carlos Seguro, Álvaro Pacheco‐Silva, Niels Olsen Saraiva Câmara,
Abstract Mesenchymal stem cells (MSCs) have regenerative properties in acute kidney injury, but their role in chronic kidney diseases is still unknown. More specifically, it is not known whether MSCs halt fibrosis. The purpose of this work was to investigate the role of MSCs in fibrogenesis using a model of chronic renal failure. MSCs were obtained from the tibias and femurs of male Wistar-EPM rats. Female Wistar rats were subjected to the remnant model, and 2|×|105 MSCs were intravenously administrated ...
Tópico(s): Wound Healing and Treatments
2009 - Oxford University Press | Stem Cells
Produção Nacional
Revisado por pares
Carla Q. Feitoza, Patrícia Semedo, Giselle Martins Gonçalves, Marcos Antônio Cenedeze, Hélady Sanders-Pinheiro, Oscar Fernando Pavão dos Santos, Richardt G. Landgraf, Álvaro Pacheco‐Silva, Niels Olsen Saraiva Câmara,
Tópico(s): Drug-Induced Hepatotoxicity and Protection
2009 - Springer Science+Business Media | Inflammation Research
Produção Nacional
Revisado por pares
Patrícia Semedo, Carolina Gardini Palasio, Cassiano Donizetti de Oliveira, Carla Q. Feitoza, Giselle Martins Gonçalves, Marcos Antônio Cenedeze, Pamella M.H. Wang, Vicente P. A. Teixeira, Marlene Antônia dos Reis, Álvaro Pacheco‐Silva, Niels Olsen Saraiva Câmara,
Therapy with stem cells has showed to be promising for acute kidney injury (AKI), although how it works is still controversial. Modulation of the inflammatory response is one possible mechanism. Most of published data relies on early time and whether the protection is still maintained after that is not known. Here, we analyzed whether immune modulation continues after 24 h of reperfusion. MSC were obtained from male Wistar rats. After 3–5 passages, cells were screened for CD73, CD90, CD44, CD45, ...
Tópico(s): Hydrogen's biological and therapeutic effects
2009 - Elsevier BV | International Immunopharmacology
Produção Nacional
Revisado por pares
Rebecca Midory Marques Monteiro, Niels Olsen Saraiva Câmara, Maurício M. Rodrigues, Fanny Tzelepis, Márcio José Damião, Marcos Antônio Cenedeze, Vicente de Paula Antunes Teixeira, Marlene Antônia dos Reis, Álvaro Pacheco‐Silva,
Ischemia reperfusion injury (IRI) is a potential contributor for the development of chronic allograft nephropathy. T cells are important mediators of injury, even in the absence of alloantigens. We performed a depletion of TCD4(+)CTLA4(+)Foxp3(+) cells with anti-CD25(PC61), a treatment with anti-GITR (DTA-1) and rat-IgG, followed by 45 min of ischemia and 24/72 h of reperfusion, and then analyzed blood urea, kidney histopathology and gene expression in kidneys by QReal Time PCR. After 24 h of reperfusion, ...
Tópico(s): Immune Cell Function and Interaction
2009 - Elsevier BV | Transplant Immunology
Produção Nacional
Revisado por pares
Ana Cristina Carvalho de Matos, Niels Olsen Saraiva Câmara, Ana Francisca Franco De Oliveira, Marcello Fabiano de Franco, Luiz A. Moura, Sônia K. Nishida, Aparecido B. Pereira, Álvaro Pacheco‐Silva,
Renal transplant patients with stable graft function and proximal tubular dysfunction (PTD) have an increased risk for chronic allograft nephropathy (CAN). In this study, we investigated the histologic pattern associated with PTD and its correlation with graft outcome. Forty-nine transplant patients with stable graft function were submitted to a biopsy. Simultaneously, urinary retinol-binding protein (uRBP) was measured and creatinine clearance was also determined. Banff's score and semi-quantitative ...
Tópico(s): Renal and Vascular Pathologies
2009 - Springer Science+Business Media | Transplant International
Produção Nacional
Revisado por pares
Niels Olsen Saraiva Câmara, W. W. Williams, Álvaro Pacheco‐Silva,
New strategies are being devised to limit the impact of renal sclerosis on graft function. Individualization of immunosuppression, specifically the interruption of calcineurin-inhibitors has been tried in order to promote better graft survival once chronic graft dysfunction has been established. However, the long-term impact of these approaches is still not totally clear. Nevertheless, patients at higher risk for tubular atrophy and interstitial fibrosis (TA/IF) development should be carefully monitored ...
Tópico(s): Chronic Kidney Disease and Diabetes
2009 - Associação Brasileira de Divulgação Científica | Brazilian Journal of Medical and Biological Research
Dora J. Borbón-González, Rafael Pacheco-Contreras, Álvaro Posada-Amarillas, J. Christian Schön, Roy L. Johnston, J.M. Montejano‐Carrizales,
We present a systematic study of the structural changes of 19-atom PdnPt19-n nanoparticles as a function of composition, modeling the interatomic interactions with the many-body Gupta potential and using a genetic algorithm to obtain the lowest energy structures for all possible compositions. Topological analysis reveals that most of the structures are based on icosahedral packings and are strongly composition dependent. The pure Pd19 nanoparticle exhibits a double icosahedral geometry, while the ...
Tópico(s): Nanocluster Synthesis and Applications
2009 - American Chemical Society | The Journal of Physical Chemistry C
Fadi G. Lakkis, Alan W. Steele, Álvaro Pacheco‐Silva, Vicki E. Rubin‐Kelley, Terry B. Strom, John R. Murphy,
Abstract The interleukin 4 (IL 4) receptor is expressed on various cells of the immune system, including T and B lymphocytes, macrophages and mast cells. We have constructed a recombinant protein, DAB 389 ‐mIL 4, that is composed of the enzymatically active and membrane translocation domains of diphtheria toxin fused to murine IL 4. We demonstrate that this fusion toxin selectively inhibits protein synsthesis in eukaryotic cells which express the murine IL 4 receptor. The cytotoxic potency of this ...
Tópico(s): Clostridium difficile and Clostridium perfringens research
1991 - Wiley | European Journal of Immunology
Cristina Díaz Gallo, Anthony M. Jevnikar, Daniel C. Brennan, Sandrine Florquin, Álvaro Pacheco‐Silva, Vicki Rubin Kelley,
T-cells have been implicated in autoimmune renal injury. To examine the role of T-cells in lupus nephritis we propagated T-cell clones from the cortical interstitium of MRL/lpr mice. All isolated kidney-infiltrating (KI) T-cell clones [6] express surface markers identical to the T-cells regulated by the lpr gene (Thy 1.2+, TCR alpha/beta +, Lyt-2-, L3T4-, B220+). Although KI T-cell clones have the same surface markers as lymph node-infiltrating (LNI) T-cells, they differ functionally. KI T-cells, but not ...
Tópico(s): Reproductive System and Pregnancy
1992 - Elsevier BV | Kidney International
Produção Nacional
Revisado por pares
Álvaro Pacheco‐Silva, Marcus Gomes Bastos, Robert A. Muggia, O. Pankewycz, Jean Nichols, John R. Murphy, Terry B. Strom, Vicki E. Rubin‐Kelley,
Abstract Insulin‐dependent diabetes mellitus (IDDM) is strikingly similar in the non‐obese diabetic (NOD) mouse and humans. In IDDM, the systematic autoimmune destruction of insulin‐producing β cells within the pancreas is dependent on autoreactive T cells. This autoimmune process can be accelerated by transferring spleen cells from diabetic donors into irradiated syngeneic NOD mice. In a previous study we established that interleukin 2 receptor (IL 2R)‐bearing cells propagated from pre‐diabetic NOD ...
Tópico(s): Diabetes Management and Research
1992 - Wiley | European Journal of Immunology
Produção Nacional
Revisado por pares
Milton Soibelmann Lapchik, Adauto Castelo Filho, José Osmar A. Pestana, Alvaro Pacheco SILVA FILHO, Sérgio Barsanti Wey,
No AccessJournal of Urology1 Apr 1992Risk Factors for Nosocomial Urinary Tract and Postoperative Wound Infections in Renal Transplant Patients: A Matched-Pair Case-Control Study Milton S. Lapchik, Adauto Castelo Filho, José Osmar A. Pestana, Álvaro P. Silva Filho, and Sérgio B. Wey Milton S. LapchikMilton S. Lapchik More articles by this author , Adauto Castelo FilhoAdauto Castelo Filho More articles by this author , José Osmar A. PestanaJosé Osmar A. Pestana More articles by this author , Álvaro P. Silva ...
Tópico(s): Central Venous Catheters and Hemodialysis
1992 - Lippincott Williams & Wilkins | The Journal of Urology
Produção Nacional
Revisado por pares
Soraia R. C. Ferreira, Valdir A. Mois s, Agostinho Tavares, Álvaro Pacheco‐Silva,
Background. Cardiovascular disease is the leading cause of death in renal transplant recipients. The purpose of this study was to determine the effects of a successful renal transplantation on left ventricular morphology and function and on the 24-hr blood pressure profile. Methods. Twenty-four patients with end-stage renal disease were prospectively studied by ambulatory blood pressure monitoring and echocardiography before and at 3, 6, and 12 months after renal transplantation. Patients were also ...
Tópico(s): Transplantation: Methods and Outcomes
2002 - Wolters Kluwer | Transplantation
Produção Nacional
Revisado por pares
Nelson Zocoler Galante, Hélio S. Júnior Tedesco, P.G.P Machado, Álvaro Pacheco‐Silva, José Osmar Medina Pestana,
Ever since the first successful kidney transplantation, the occurrence of acute rejection has been a dominant risk factor for adverse graft outcome, as it is associated with reduced graft survival and the development of chronic transplant dysfunction. Although the majority of acute renal allograft rejection episodes can be adequately treated with glucocorticoid therapy, 25 to 30% of the rejection episode cannot be reversed with glucocorticoids alone. At present, the diagnosis of steroid resistance ...
Tópico(s): Liver Disease and Transplantation
2002 - Elsevier BV | Transplantation Proceedings
Produção Nacional
Revisado por pares
Carla Q. Feitoza, Hélady Sanders-Pinheiro, Marcos Antônio Cenedeze, Niels Olsen Saraiva Câmara, Álvaro Pacheco‐Silva,
Acute kidney injury (AKI) causes considerable morbidity and mortality, particularly in the case of post-cardiac infarction or kidney transplantation; however, the site-specific accumulation of small molecule reno-protective agents for AKI has often proved ineffective due to dynamic fluid and solute excretion and non-selectivity, which impedes therapeutic efficacy. This article reviews the current status and future trajectories of renal nanomedicine research for AKI management from pharmacological ...
Tópico(s): Organ Transplantation Techniques and Outcomes
2002 - Elsevier BV | Transplantation Proceedings
Produção Nacional
Revisado por pares
Carolina Miranda, Cláudio S. Melaragno, Niels Olsen Saraiva Câmara, Álvaro Pacheco‐Silva, José Osmar Medina Pestana,
Tópico(s): Potassium and Related Disorders
2002 - Elsevier BV | Transplantation Proceedings
Produção Nacional
Revisado por pares
R.G da Silva, Valter C. Lima, Jorge Eduardo de Amorim, P.G.P Machado, Álvaro Pacheco‐Silva, José Osmar Medina Pestana,
Tópico(s): Congenital Heart Disease Studies
2002 - Elsevier BV | Transplantation Proceedings
Produção Nacional
Revisado por pares
Pamella Huey Mei Wang, Marcos Antônio Cenedeze, Gabriela Campanholle, Denise Maria Avancini Costa Malheiros, Hugo Arruda de Moura Torres, João Bosco Pesquero, Álvaro Pacheco‐Silva, Niels Olsen Saraiva Câmara,
The Kallikrein–kinin system works through activation of two receptors. One constitutive, named B2 receptor (B2R) and another inducible, denominated B1 receptor (B1R). In renal fibrosis, B2R receptor activation appears to be protective, however B1R participation is unveiled. The aim of this study was to analyze how the deletion of the B1R would modify tissue responses after unilateral ureteral obstruction (UUO). For that, B1R knockout (B1KO) and wild-type mice (B1B2WT) were subjected to UUO and sacrificed ...
Tópico(s): Blood Coagulation and Thrombosis Mechanisms
2008 - Elsevier BV | International Immunopharmacology
Produção Nacional
Revisado por pares
Vanessa Nunes de Paiva, Rebecca Midory Marques Monteiro, Vilmar de Paiva Marques, Marcos Antônio Cenedeze, Vicente de Paula Antunes Teixeira, Marlene Antônia dos Reis, Álvaro Pacheco‐Silva, Niels Olsen Saraiva Câmara,
Renal ischemia and reperfusion injury (IRI) is considered an inflammatory syndrome. To move forward in its pathogenesis, we exploited the role of several cytokines on renal damages triggered by IRI. Specifically to evaluate the role of Th1 immune profile in this system, IL-12, IFN-γ, and IFN-γ/IL-12 deficient (KO) mice on C57BL/6 background and their controls were subjected to IRI. In each group, blood and kidney samples were harvested. Renal function was evaluated by serum creatinine and renal morphometric ...
Tópico(s): Thermal Regulation in Medicine
2008 - Elsevier BV | International Immunopharmacology
Produção Nacional
Revisado por pares
Carla Q. Feitoza, Giselle Martins Gonçalves, Patrícia Semedo, Marcos Antônio Cenedeze, Hélady Sanders-Pinheiro, Felipe Caetano Beraldo, Oscar Fernando, Pavão dos Santos, Vicente de Paula Antunes Teixeira, Marlene Antônia dos Reis, Marilda Mazzali, Álvaro Pacheco‐Silva, Niels Olsen Saraiva Câmara,
Ischemia and reperfusion injury (IRI) contributes to the development of chronic interstitial fibrosis/tubular atrophy in renal allograft patients. Cyclooxygenase (COX) 1 and 2 actively participate in acute ischemic injury by activating endothelial cells and inducing oxidative stress. Furthermore, blockade of COX 1 and 2 has been associated with organ improvement after ischemic damage. The aim of this study was to evaluate the role of COX 1 and 2 in the development of fibrosis by performing a COX 1 ...
Tópico(s): Cerebrovascular and genetic disorders
2008 - BioMed Central | Molecular Medicine
Produção Nacional
Revisado por pares
Pamella H. M. Wang, Gabriela Campanholle, Marcos Antônio Cenedeze, Carla Q. Feitoza, Giselle Martins Gonçalves, Richardt G. Landgraf, Sônia Jancar, João Bosco Pesquero, Álvaro Pacheco‐Silva, Niels Olsen Saraiva Câmara,
Previously we have demonstrated that bradykinin B1 receptor deficient mice (B1KO) were protected against renal ischemia and reperfusion injury (IRI). Here, we aimed to analyze the effect of B1 antagonism on renal IRI and to study whether B1R knockout or antagonism could modulate the renal expression of pro and anti-inflammatory molecules. To this end, mice were subjected to 45 minutes ischemia and reperfused at 4, 24, 48 and 120 hours. Wild-type mice were treated intra-peritoneally with antagonists ...
Tópico(s): Hemophilia Treatment and Research
2008 - Public Library of Science | PLoS ONE