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John Dugdale, Sebastian Hamilton Westminster Correspondent, Barbara Hall, Judith O'Reilly Education Correspondent, Nigel Botherway, Sir Anthony Bamford Chairman, Lucas Hollweg, Glynn Christian, Fred Redwood, Anon, John Peter, Walter F Stowy, Henry Keswick Chairman, Emma Moore, Amanda Ursell, Jonathan Miller, Jim Munro football editor, Lesley White, John Hersey, Lawrie Madden, John Jay, John Lennon, Susan d'Arcy, Ena Wogin, John Knapton, Frank Whitford, Karen Woolfson, Ferdinand Mount, Sir John Nott Chairman, Addison de Witt, Howell Wilson-Price, Thierry Oberlé, David Smith, Sally O'Reilly, Sally Payne, Nick Pitt, Neil Wormald, Sebastian Hamilton, Andrew Sullivan, Sir Stanley Kalms Chairman, Clive Davis, Mary Wilson, Karen Robinson, Mark Reynolds, Andrew Grice Political Editor, Patrick Masters, John Lawless, Tony Allen-Mills, Martin James, Elaine Ives-Cameron, Jennifer, Irwin Stelzer, Peter Wilson, Roger Anderson, George Turnbull, Stephen Hayward, Lord Young Chairman, David Dougill, Karen Swann McLeod, Vinny Lee, Richard Gregory Professor, David Hewson, Tony Latham, Sir Christopher Lewinton Chairman, Chris Hastings, Harvey Porlock, Margaret Coles, Hugh Canning, Jeremy Clarkson, David Cairns, Stewart Lee, Peter Conradi, Carole Hodge, Louise Taylor, Peter Bills, Michael Jones, Anita Chaudhuri, Peter Millar, Donald Foreman Secretary, George Perry, Sir John Craven Chairman, Kevin Connolly, Graham Otway, Charlotte Atkins, Nick Gardner, Shelley von Strunckel, Sir Robert McAlpine, Paul Nuki Consumer Affairs Correspondent, Rob Steen, Paul Ham, Stuart Wavell, Sir Emmanucl Kaye Chairman, Paul Donovan, Frances Spalding, William Shawcross, Sue Lawrence, Hugh McIlvanney, Mary Lewis, Paul Driver, Lawrence Millman, Michael Woodhead, Garth Pearce, David Thomas, Caroline Scott, Ian Hawkey, Ian Chadband, Jacqui Ripley, Christopher Morgan, David Leppard, Michael Jenner, Ross Clark, Neil Harman, Kirstie Hamilton, Tom Shone, Mark Edwards, Nicholas Harling, Rufus Olins, Sir Michael Edwardes, Ian McAlpine Director, Mark Austin, David Lawrenson, Stephen Amidon, Nicholas Rufford Home Affairs Editor, Lord Hanson, Lord Wolfson Chairman, Andy Goldberg, Hugo Barnacle, Rubert Steiner, Michael Brinton Chairman, Lois Rogers, Dr Maurice Dixson Chief Excecutive, Raymond Keene, Jonathan Leake Environment Correspondent, John Karter, Cosmo Landesman, Zoe Brennan, Andrew Roberts, Marie Colvin, Debbie Hill, Diana Wright, Ken Symon, Robert Beerman, Helen Hawkins, Stephen Jones, Richard Evans, Uzi Mahnaimi, Nick Cain, Natalie Graham, Andrew Smith, Tim Moorey, Andrew Grice, Nick Hodges, Martin Searby, Rob Ryan, Claire Oldfield, Sir Richard George Chairman, David Walsh, Colin McDowell, Bryan Appleyard, Lois Rogers Medical Correspondent, Richard Balls, Stephen Armstrong, Andrew Jack, Garry Weston Chairman, Peter Taylor, Paul Kavanagh, Mark Prigg, George Walden, Josh Salzmann, D H, Sir Rocco Forted, David Windle, Garth Alexander, Robert Sandair, Roland White, Rachel Cooke, Danny Roth, Susan Greenfield Professor, Maurice Chittenden, Kevin Pratt, Jack Hall, Andrew Lorenz Business Editor, Anthony Julius, Mark Franchetti, Craig Lord, Ray Hutton, Jon Ungoed-Thomas, Rufus Olins Deputy City Editor, David Hutchinson, Peter Watts, Tara Palmer-Tomkinson, Mrs D Mitchell, Fiona Matthias, Michael Sheridan, Sean Hargrave, Christine Webb, John Harlow, David Green, Matthew Lynn, Godfrey Smith, Donald Macgillivray, Nia Williams, Mandy Payne, Andrew Alderson, John Waples, Mary Cecil Pook, Dr D Allen, James Herbert, Matthew Campbell, David Hutcheon, Rajeev Syal, Naomi Caine Deputy Money Editor, Chrissy Iley, Robert Johnston, Stephen Pettitt, Cherry Norton, Andrew Lorenz, Naomi Caine, Liam Clarke, David Wickers, Hazel Courteney, Roger Dobson, Abdel Hamid Aoun, Graham Sheffield Arts Director, Mark Hodson, Steve Connor, Eric Reeves, Rupert Steiner, Chris Lightbown, Anthony Powell, Sunningdale Chairman, John Phillips, Kevin Snow, David Smith Economics Editor, Terence Conran, Christopher Miller Chairman, Peter Cooper, Anthony Howard, Carey Scott, Joanna Simon, Kelly Finn, Dan Pearson, Sebastian Hamilton Westminister Correspondent, Boris Schapiro, Adam Parsons,

... to bulldoze green belt for millions of homes Núrnberg Messe Opus technology British Gas Spencers raided family ... a Legend Soul mate Join The Fun Club! FC Funfax Contents Stamina The horsy couple Who do ...

1998 - Gale Group | Sunday Times HA GDA

Carta

David S. Geller,

... hypertension. Med Clin North Am. Jan 2004; 88: 1–18, vii.CrossrefMedlineGoogle Scholar4 Gong M, Zhang H, Schulz H, Lee YA, Sun K, Bahring S, Luft FC, Nurnberg P, Reis A, Rohde K, Ganten D, Hui ...

Tópico(s): Sodium Intake and Health

2004 - Lippincott Williams & Wilkins | Hypertension

Artigo

H. Meier,

... aus. Er schoss das 1 : 0 für den 1. FC Nürnberg. Die Freude währte allerdings nur kurze Zeit. In ...

Tópico(s): Bone fractures and treatments

2008 - Georg Thieme Verlag | B&G Bewegungstherapie und Gesundheitssport

Artigo

Ekaterina Papoucheva, Aline Dumuis, Michèle Sebben, Diethelm W. Richter, Evgeni Ponimaskin,

... purified, and amplified as described previously (44Veit M. Nurnberg B. Spicher K. Harteneck C. Ponimaskin E. Schultz G. Schmidt M.F. FEBS Lett. 1994; 339: 160-164Google Scholar). All mutants were verified by dideoxy DNA sequencing of the final plasmid. Metabolic Labeling and Immunoprecipitation—Spodoptera frugiperda (Sf.9) cells were grown in TC-100 medium supplemented with 10% fetal calf serum (FCS) and 1% penicillin/streptomycin (complete TC-100). For expression, Sf. ...

Tópico(s): Neuropeptides and Animal Physiology

2004 - Elsevier BV | Journal of Biological Chemistry

Artigo

Triantafyllos Chavakis, Antje Willuweit, Florea Lupu, Klaus T. Preissner, Sandip M. Kanse,

... this work was supported by grants (Pr 327/1-4) from the Deutsche Forschungsgemeinschaft (Bonn, Germany) and the Novartis-Foundation (Nürnberg, Germany). This work is part of the MD/PhD-thesis of T.C. at the Institute for Biochemistry, Department of Medicine, Justus-Liebig-Universität, Giessen, Germany. Abbreviations: bFGF: basic fibroblast growth factor, GPI: glycosyl-phosphatidylinositol, FCS: fetal calf serum, HUVEC: human umbilical vein endothelial ...

Tópico(s): Peptidase Inhibition and Analysis

2001 - Thieme Medical Publishers (Germany) | Thrombosis and Haemostasis

Revisão

Friedrich C. Luft,

... U, Rohde K, Schuster H, Junghans C, Luft FC, Nürnberg P, Reich JG. Haplotypes and SNPs in 13 ...

Tópico(s): Pharmacogenetics and Drug Metabolism

2004 - Lippincott Williams & Wilkins | Hypertension

Artigo

Bootz Anna, Winkler Thomas, Mach Michael,

Event Abstract Back to Event Monocytes rather than NK-cells contribute to antibody mediated protection from MCMV infection Anna Bootz1*, Thomas Winkler2 and Michael Mach1 1 Institute for Clinical and Molecular Virology, Friedrich-Alexander university Erlangen-Nürnberg,, Germany 2 Nikolaus-Fiebiger-Center for Molecular Medicine, Friedrich-Alexander university Erlangen-Nürnberg, Germany Cytomegalovirus is a clinically important pathogen. Previously we could show using murine cytomegalovirus (MCMV) ...

Tópico(s): T-cell and B-cell Immunology

2013 - Frontiers Media | Frontiers in Immunology

Carta

Hussam Al‐Kateb, Ekkehard K.F. Bautz, Friedrich C. Luft, Sylvia Bähring,

... Bahring S, Hoffmann K, Strauch K, Busjahn A, Nurnberg G, Jouma M, Bautz EK, Dresel HA, Luft FC. Mutation in the ARH gene and a chromosome ...

Tópico(s): RNA and protein synthesis mechanisms

2003 - Lippincott Williams & Wilkins | Circulation Research

Artigo

Jean‐Pierre Allam, Elisabeth Klein, Thomas Bieber, Natalija Novak,

... Bad Soden, Germany). Heat-inactivated fetal calf serum (FCS), antibiotics/antimycotics and RPMI-1640 with Glutamax were all from Invitrogen (Carlsbad, California). Granulocyte macrophage colony stimulating factor (GM-CSF) was purchased from Novartis Pharma (Nürnberg, Germany), tumor necrosis factor (TNF-β, stem cell ...

Tópico(s): Immune Cell Function and Interaction

2004 - Elsevier BV | Journal of Investigative Dermatology

Artigo

Julia Skokowa, Syed R. Ali, Olga Felda, Varsha Kumar, Stephanie Konrad, Nelli Shushakova, Reinhold Schmidt, Roland P. Piekorz, Bernd Nürnberg, Karsten Spicher, Lutz Birnbaumer, Jörg Zwirner, Jill Claassens, Josef S. Verbeek, Nico van Rooijen, Jörg Köhl, J. Engelbert Gessner,

Complement and FcgammaR effector pathways are central triggers of immune inflammation; however, the exact mechanisms for their cooperation with effector cells and their nature remain elusive. In this study we show that in the lung Arthus reaction, the initial contact between immune complexes and alveolar macrophages (AM) results in plasma complement-independent C5a production that causes decreased levels of inhibitory FcgammaRIIB, increased levels of activating FcgammaRIII, and highly induced FcgammaR- ...

Tópico(s): T-cell and B-cell Immunology

2005 - American Association of Immunologists | The Journal of Immunology

Artigo

Rachael B. Cowerd, Melissa M. Asmar, J. McKee Alderman, Elizabeth A. Alderman, Alaina L. Garland, Walker H. Busby, Wanda M. Bodnar, Ivan Rusyn, Benjamin D. Medoff, Roland Tisch, Elizabeth J. Mayer‐Davis, James A. Swenberg, Steven H. Zeisel, Terry P. Combs,

... MJ Levine B Lieberman A Lim KL Lin FC Liou W Liu LF Lopez-Berestein G López-Otín C Lu B Macleod KF Malorni W Martinet W Matsuoka K Mautner J Meijer AJ Meléndez A Michels P Miotto G Mistiaen WP Mizushima N Mograbi B Monastyrska I Moore MN Moreira PI Moriyasu Y Motyl T Münz C Murphy LO Naqvi NI Neufeld TP Nishino I Nixon RA Noda T Nürnberg B Ogawa M Oleinick NL Olsen LJ Ozpolat ...

Tópico(s): Adipose Tissue and Metabolism

2010 - Elsevier BV | American Journal Of Pathology

Artigo

E. Lang, A. Spitzer, D. Claus, B. Neundörfer, Hermann O. Handwerker,

... und Biokybernetik und Neurologische Klinik der Universität Erlangen-Nürnberg, GermanyNeurologische Klinik der Universität Erlangen, Schwabachanlage 6, D- ... und Biokybernetik und Neurologische Klinik der Universität Erlangen-Nürnberg, GermanySearch for more papers by this authorD. Claus, ... und Biokybernetik und Neurologische Klinik der Universität Erlangen-Nürnberg, GermanySearch for more papers by this authorB. Neundörfer, ... und Biokybernetik und Neurologische Klinik der Universität Erlangen-Nürnberg, GermanySearch for more papers by this authorH. O. ...

Tópico(s): Musculoskeletal pain and rehabilitation

1995 - Wiley | Acta Neurologica Scandinavica

Artigo

Stephanie Konrad, Syed R. Ali, Kristina Wiege, Shahzad N. Syed, Linda Engling, Roland P. Piekorz, Emilio Hirsch, Bernd Nürnberg, Reinhold Schmidt, J. Engelbert Gessner,

... Shushakova N. Schmidt R.E. Piekorz R.P. Nürnberg B. Spicher K. Birnbaumer L. Zwirner J. Claassens ... Shushakova N. Schmidt R.E. Piekorz R.P. Nürnberg B. Spicher K. Birnbaumer L. Zwirner J. Claassens ... PubMed Scopus (1323) Google Scholar, 20Piekorz R.P. Nürnberg B. Phospholipid Kinases. Elsevier Science Publishing Co., Inc., ... Shushakova N. Schmidt R.E. Piekorz R.P. Nürnberg B. Spicher K. Birnbaumer L. Zwirner J. Claassens ... Shushakova N. Schmidt R.E. Piekorz R.P. Nürnberg B. Spicher K. Birnbaumer L. Zwirner J. Claassens ...

Tópico(s): Complement system in diseases

2008 - Elsevier BV | Journal of Biological Chemistry

Artigo

Serge X. Cohen,

... Hashemolhosseini Said Hashemolhosseini Institut für Biochemie, Universität Erlangen-Nürnberg, Fahrstrasse 17, D-91054 Erlangen, Germany Search for ... Kilian Karin Kilian Institut für Biochemie, Universität Erlangen-Nürnberg, Fahrstrasse 17, D-91054 Erlangen, Germany Search for ... Wegner Michael Wegner Institut für Biochemie, Universität Erlangen-Nürnberg, Fahrstrasse 17, D-91054 Erlangen, Germany Search for ... Hashemolhosseini Said Hashemolhosseini Institut für Biochemie, Universität Erlangen-Nürnberg, Fahrstrasse 17, D-91054 Erlangen, Germany Search for ...

Tópico(s): RNA modifications and cancer

2003 - Springer Nature | The EMBO Journal

Artigo

Christel Krueger, Klaus Pfleiderer, Wolfgang Hillen, Christian Berens,

... Wolfgang Hillen & Christian BerensChristel KruegerFriedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany, Klaus PfleidererFriedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany, Wolfgang HillenFriedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany & Christian Berens*Address correspondence to: Christian Berens, Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany. e-mail: E- ... cberens@biologie.uni-erlangen.deFriedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, GermanyPublished Online:6 Jun 2018https://doi.org/ ...

Tópico(s): RNA Interference and Gene Delivery

2004 - Future Science Ltd | BioTechniques

Artigo

Xuping Wang, Michael Schunck, Karl‐Josef Kallen, Claudia Neumann, Christian Trautwein, Stefan Rose‐John, Ehrhardt Proksch,

... 1992; 72: 165-168PubMed Google Scholar), lupus erythematosus (Nurnberg et al., 1995Nurnberg W. Haas N. Schadendorf D. ... of the heavy chain of human IgG1, gp130-Fc, has been shown to specifically inhibit processes mediated ... 6-deficient mice, Hyper-IL-6 and gp130-Fc we have analyzed the role of the IL- ... bound or the soluble IL-6R, the gp130-Fc fusion protein, specific inhibitor of trans-signalling via ... 24 h (Figure 6). Topical application of gp130-Fc in IL-6-deficient mice did not significantly ...

Tópico(s): Signaling Pathways in Disease

2004 - Elsevier BV | Journal of Investigative Dermatology

Artigo

A al-Aoukaty, Bent Rolstad, Azzam A. Maghazachi,

... Malek D. Stoyanova S. Vanhaesebroeck B. Dhand R. Nurnberg B. Gierschik P. Seedorf K. Hsuan J.S. ... Malek D. Stoyanova S. Vanhaesebroeck B. Dhand R. Nurnberg B. Gierschik P. Seedorf K. Hsuan J.S. ... Malek D. Stoyanova S. Vanhaesebroeck B. Dhand R. Nurnberg B. Gierschik P. Seedorf K. Hsuan J.S. ... inside these cells. These include: (i) NK cell Fc receptors (32Procopio A.D. Paolini R. Vevhio I ...

Tópico(s): T-cell and B-cell Immunology

1997 - Elsevier BV | Journal of Biological Chemistry

Artigo

L. Neff, Mirjam B. Zeisel, Vanessa A. Druet, Ken Takeda, Jean‐Paul Klein, Jean Sibilia, Dominique Wachsmann,

... M.F. Daulerio A.J. Covington M.B. Nurnberg S.A. Mafolda R.L. Trzaskos J.M. ... media (RPMI 1640 and M199), fetal calf serum (FCS), penicillin, streptomycin, amphotericin B, Taq DNA polymerase, dNTPs, ... 0.25 μg/ml), and 20% heat-inactivated FCS (complete medium). After overnight culture, non-adherent cells ... flasks in complete medium containing 10% heat-inactivated FCS. Between the third and the tenth passages, during ... 1%), sodium pyruvate (1%), and 10% heat-inactivated FCS. FAK+/+ cells were transfected with FRNK-YCam (27Giannone ...

Tópico(s): Peptidase Inhibition and Analysis

2003 - Elsevier BV | Journal of Biological Chemistry

Artigo

Karine Rutault, Catherine A. Hazzalin, Louis C. Mahadevan,

... M.F. Daulerio A.J. Covington M.B. Nurnberg S.A. Magolda R.L. Trzaskos J.M. ... salt solution were purchased from Life Technologies, Inc. FCS was obtained from Globepharm (Guildford, UK). LPS (Escherichia ... days and then starved overnight in 0.5% FCS-RPMI in the presence of 5 nm TPA ... to adhere (5 × 106 cells/ml) in 10% FCS-RPMI. After 90 min at 37 °C, 6% ... quiescent by an overnight incubation in 0.5% FCS-RPMI before stimulation for MAPK analysis or Northern ... LPS, unless differently noted, in RPMI containing 1% FCS. TNF-α levels were detected using the Duoset ...

Tópico(s): Immune Response and Inflammation

2001 - Elsevier BV | Journal of Biological Chemistry

Artigo

Bernd Wrackmeyer, Hans‐Jörg Schanz, Matthias Hofmann, Paul von Ragué Schleyer,

... Matthias Hofmann Institut für Organische Chemie, Universität Erlangen-Nürnberg, Henkestr. 42, D-91054 Erlangen, Fax: Int. code + ... email protected] Institut für Organische Chemie, Universität Erlangen-Nürnberg, Henkestr. 42, D-91054 Erlangen, Fax: Int. code + ... Matthias Hofmann Institut für Organische Chemie, Universität Erlangen-Nürnberg, Henkestr. 42, D-91054 Erlangen, Fax: Int. code + ... email protected] Institut für Organische Chemie, Universität Erlangen-Nürnberg, Henkestr. 42, D-91054 Erlangen, Fax: Int. code + ...

Tópico(s): Luminescence and Fluorescent Materials

1998 - Wiley | European Journal of Inorganic Chemistry

Artigo

H. Stadick, Bernhard Stockmeyer, R.-B. Kühn, K. M. Schrott, J. R. Kalden, Martin J. Glennie, Jan G. J. van de Winkel, Martin Gramatzki, Thomas Valerius, D. ELS ̈ASSER,

... of Medicine III and Urology, University of Erlangen-Nürnberg, Erlangen, Germany, Tenovus Research Laboratory, University of Southampton, ...

Tópico(s): CAR-T cell therapy research

2002 - Lippincott Williams & Wilkins | The Journal of Urology

Artigo

Markus Porst, Christian Plank, Beate Bieritz, E. Konik, Hans Fees, Jörg Dötsch, Karl F. Hilgers, Dieter P. Reinhardt, Andrea Hartner,

... from the Faculty of Medicine, University of Erlangen-Nürnberg and a grant from the Canadian Institutes of ...

Tópico(s): Cell Adhesion Molecules Research

2006 - Elsevier BV | Kidney International

Carta

Katharina Hermes, Pascal Schneider, Peter Krieg, Anh Thu Dang, Kenneth Huttner, Holm Schneider,

... Dr. med." from the Friedrich-Alexander-Universität Erlangen-Nürnberg. This study was supported by grants from the ...

Tópico(s): Urological Disorders and Treatments

2014 - Elsevier BV | Journal of Investigative Dermatology

Artigo

LI Zhen-yu, Guoying Zhang, Guy C. Le Breton, Xiaopei Gao, Asrar B. Malik, Xiaoping Du,

... Malek D. Stoyanova S. Vanhaesebroeck B. Dhand R. Nurnberg B. et al.Science. 1995; 269: 690-693Crossref ... Malek D. Stoyanova S. Vanhaesebroeck B. Dhand R. Nurnberg B. et al.Science. 1995; 269: 690-693Crossref ... Malek D. Stoyanova S. Vanhaesebroeck B. Dhand R. Nurnberg B. et al.Science. 1995; 269: 690-693Crossref ... protein-coupled agonists, but only affected the GPIV/Fc receptor γ pathway (39Watanabe N. Nakajima H. Suzuki ...

Tópico(s): Antiplatelet Therapy and Cardiovascular Diseases

2003 - Elsevier BV | Journal of Biological Chemistry

Artigo

Cornelia Czupalla, Hannu Mansukoski, Thilo Riedl, Dorothee Thiel, Eberhard Krause, Bernard Hoflack,

... Promega, Madison, WI) as described previously (15Czupalla C. Nürnberg B. Krause E. Analysis of class I phosphoinositide ...

Tópico(s): Bone and Dental Protein Studies

2005 - Elsevier BV | Molecular & Cellular Proteomics

Carta

Sonja Koch, Anna Graser, Hooman Mirzakhani, Theodor Zimmermann, Volker O. Melichar, Marco Wölfel, Damien C. Croteau‐Chonka, Benjamin A. Raby, Scott T. Weiss, Susetta Finotto,

... ethics committee of the Friedrich-Alexander University Erlangen-Nürnberg, Germany (reference no. 4435), and is also registered ...

Tópico(s): Antimicrobial Peptides and Activities

2016 - Elsevier BV | Journal of Allergy and Clinical Immunology

Revisão

John H. Kehrl,

... 98Spicher K Kalkbrenner F Zobel A Harhammer R Nurnberg B Soling A Schultz G G12 and G13 ...

Tópico(s): Cytokine Signaling Pathways and Interactions

1998 - Cell Press | Immunity

Artigo

Vinzenz Oji, Katja‐Martina Eckl, Karin Aufenvenne, Marc Nätebus, T. Tarinski, Katharina Ackermann, Natalia Seller, Dieter Metze, Gudrun Nürnberg, Regina Fölster‐Holst, Monika Schäfer‐Korting, Ingrid Haußer, Heiko Traupe, Hans Christian Hennies,

... cultivated in DMEM with 10% fetal calf serum (FCS), 100 IU/ml penicillin, and 100 μg/ml ... pH with NaOH, 2.5 × 105 NHDF in FCS was added, and the mixture was poured into ...

Tópico(s): Advancements in Transdermal Drug Delivery

2010 - Elsevier BV | The American Journal of Human Genetics

Artigo

Charlotte Rehfeldt,

... have been considered.REFERENCES1 Rehfeldt C, Ender K, Nürnberg K, Hackl W. Cellular aspects of the repartitioning ... 2007. Google Scholar2. Hikida RS, Staron RS, Hagerman FC, Walsh S, Kaiser E, Shell S, Hervey S. ... RS, Walsh S, Barylski N, Campos G, Hagerman FC, Staron RS. Is hypertrophy limited in elderly muscle ...

Tópico(s): Muscle metabolism and nutrition

2007 - American Physiological Society | Journal of Applied Physiology