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Artigo Acesso aberto Revisado por pares

Maria T. Bengoechea-Alonso, Johan Ericsson,

... identify a novel phosphorylation site in SREBP1a, Ser-434, which regulates the Fbw7-dependent degradation of SREBP1. ... form of endogenous SREBP1 is phosphorylated on Ser-434. Glycogen synthase kinase-3 phosphorylates Ser-434, and the phosphorylation of this residue is attenuated ... response to insulin signaling. Interestingly, phosphorylation of Ser-434 promotes the glycogen synthase kinase-3-dependent phosphorylation ... 430 and destabilizes SREBP1. Consequently, mutation of Ser-434 blocks the interaction between SREBP1 and Fbw7 and ...

Tópico(s): Peroxisome Proliferator-Activated Receptors

2009 - Elsevier BV | Journal of Biological Chemistry

Artigo Revisado por pares

Hiroyuki Ohba, Norihiro Harada, Shingo Nishiyama, Takeharu Kakiuchi, Hideo Tsukada,

... Photonics K. K., 5000 Hirakuchi, Hamakita, Hamamatsu, Shizuoka 434-8601, JapanSearch for more papers by this authorNorihiro ... Photonics K. K., 5000 Hirakuchi, Hamakita, Hamamatsu, Shizuoka 434-8601, JapanSearch for more papers by this authorShingo ... Photonics K. K., 5000 Hirakuchi, Hamakita, Hamamatsu, Shizuoka 434-8601, JapanSearch for more papers by this authorTakeharu ... Photonics K. K., 5000 Hirakuchi, Hamakita, Hamamatsu, Shizuoka 434-8601, JapanSearch for more papers by this authorHideo ... Photonics K. K., 5000 Hirakuchi, Hamakita, Hamamatsu, Shizuoka 434-8601, JapanCentral Research Laboratory, Hamamatsu Photonics K.K., ...

Tópico(s): Neuroscience and Neuropharmacology Research

2009 - Wiley | Synapse

Livro

IEEE Xplore

Artigo Acesso aberto Revisado por pares

Gerald B. Koudelka,

The extent of DNA bending induced by 434 repressor, its amino terminal DNA binding domain (R1–69), and 434 Cro was studied by gel shift assay. The results show that 434 repressor and R1–69 bend DNA to the same extent. 434 Cro-induced DNA bends are similar to those seen with the 434 repressor proteins. On ∼265 base pair fragments, the ... coli (CRP) produces larger mobility shifts than does 434 repressor. This indicates that the 434 proteins bend DNA to a much smaller extent ... effects of central operator sequence on intrinsic and 434 protein-induced DNA bending was also examined by ...

Tópico(s): Cancer Research and Treatments

1991 - Oxford University Press | Nucleic Acids Research

Artigo Acesso aberto Revisado por pares

Hiroshi Tokumitsu, Hiroyuki Inuzuka, Yumi Ishikawa, Ryōji Kobayashi,

... 15818Google Scholar). GST fused with CaM-KKα-(126–434) and CaM-KKβ-(162–470) was constructed by ... by PCR using specific primers (CaM-KKα-(126–434): sense primer, 5′-TCTCTAGAGAACCAGTACAAGCTG-3′; antisense primer, 5′- ... GST-CaM-KKβ-(162–374)/CaM-KKα-(339–434) and GST-CaM-KKβ-(162–303)/CaM-KKα-(268–434) were constructed by replacement of the corresponding fragment ... GST-CaM-KKβ-(162–271)/CaM-KKα-(236–434) was constructed by PCR amplification of each cDNA ... 3′ for the amplification of CaM-KKα-(237–434)) and pyrobest DNA polymerase, followed by the insertion ...

Tópico(s): Melanoma and MAPK Pathways

2003 - Elsevier BV | Journal of Biological Chemistry

Artigo Revisado por pares

Qi Huang, Steven A. Fisher, Frank V. Brozovich,

... E. Science. 1999; 286: 1583-1587Crossref PubMed Scopus (434) Google Scholar, 9.Lincoln T.M. Dey N. ... E. Science. 1999; 286: 1583-1587Crossref PubMed Scopus (434) Google Scholar, 15.Etter E.F. Eto M. ... E. Science. 1999; 286: 1583-1587Crossref PubMed Scopus (434) Google Scholar, 2.Khatri J.J. Joyce K. ... E. Science. 1999; 286: 1583-1587Crossref PubMed Scopus (434) Google Scholar). In this study, we tested the ... E. Science. 1999; 286: 1583-1587Crossref PubMed Scopus (434) Google Scholar) containing 50 mm Tris-HCl (pH ...

Tópico(s): Mitochondrial Function and Pathology

2003 - Elsevier BV | Journal of Biological Chemistry

Revisão Revisado por pares

Annelyse Duvoix, Martine Schmitz, Michaël Schnekenburger, Mario Dicato, Franck Morceau, M M Galteau, Marc Diederich,

... 1511 Luxembourg, Luxembourg Tel.: +352 46 66 44 434; Fax: +352 46 66 44 438Search for more ... 1511 Luxembourg, Luxembourg Tel.: +352 46 66 44 434; Fax: +352 46 66 44 438Search for more ... 1511 Luxembourg, Luxembourg Tel.: +352 46 66 44 434; Fax: +352 46 66 44 438Search for more ... 1511 Luxembourg, Luxembourg Tel.: +352 46 66 44 434; Fax: +352 46 66 44 438Search for more ... 1511 Luxembourg, Luxembourg Tel.: +352 46 66 44 434; Fax: +352 46 66 44 438Search for more ... 1511 Luxembourg, Luxembourg Tel.: +352 46 66 44 434; Fax: +352 46 66 44 438Search for more ...

Tópico(s): Acute Lymphoblastic Leukemia research

2003 - Wiley | BioFactors

Livro

Project Gutenberg

Artigo Acesso aberto Revisado por pares

Paul A. Carlson, Gerald B. Koudelka,

The repressor protein of bacteriophage 434 binds to DNA as a dimer of identical subunits. Its strong dimerization is mediated by the carboxyl-terminal domain. Cooperative interactions ... the overproduction of the carboxyl-terminal domain of 434 repressor. The protein encoded by this plasmid is ... are unable to be lysogenized by wild-type 434 phage, whereas control cells are lysogenized at an ... 2:1 ratio of CT-1 to intact 434 repressor, purified CT-1 protein prevents the formation of complexes between 434 repressor and its OR1 binding site. Second, cross- ...

Tópico(s): DNA and Nucleic Acid Chemistry

1994 - American Society for Microbiology | Journal of Bacteriology

Artigo Acesso aberto Revisado por pares

Fabrizio Villa, Jürgen Goebel, Fatema H. Rafiqi, Mária Deák, Jacob Thastrup, Dario R. Alessi, Daan M. F. van Aalten,

... domain. A construct covering the CCT domain (residues 434–527) readily yielded soluble expression in Escherichia coli ... Ribbon representation of CCT domain of OSR1 (residues 434–527) coloured in blue and red (β-strands ... view of the CCT domain of OSR1 (residues 434–527) coloured in blue and red (β-strands ... domain and the OSR1 CCT domain mutants (residues 434–527) to a biotinylated RFQV-containing peptide derived ... hypertension. Methods Cloning, expression and crystallization of OSR1 (434–527). A fragment of OSR1 (434–527) was ...

Tópico(s): Enzyme Structure and Function

2007 - Springer Nature | EMBO Reports

Periodico Acesso aberto

Periódicos nacionais

Artigo Acesso aberto Revisado por pares

Mirna Z. Ilic, Charlotte J. East, Fraser M. Rogerson, Amanda Fosang, Christopher J. Handley,

... Majumdar M.K. Morris E.A. Nature. 2005; 434: 644-648Crossref PubMed Scopus (1010) Google Scholar, 6Little ... Fourie A.M. Fosang A.J. Nature. 2005; 434: 648-652Crossref PubMed Scopus (758) Google Scholar, 10Tortorella ... Fourie A.M. Fosang A.J. Nature. 2005; 434: 648-652Crossref PubMed Scopus (758) Google Scholar, 22East ... Majumdar M.K. Morris E.A. Nature. 2005; 434: 644-648Crossref PubMed Scopus (1010) Google Scholar, 9Stanton ... Fourie A.M. Fosang A.J. Nature. 2005; 434: 648-652Crossref PubMed Scopus (758) Google Scholar). Furthermore ...

Tópico(s): Chemokine receptors and signaling

2007 - Elsevier BV | Journal of Biological Chemistry

Periodico Acesso aberto

PubMed Central (PMC)

Artigo Revisado por pares

Frederic D. Bushman,

... repressor and Cro proteins of the related phage 434. The start sites of transcription of the divergently oriented promoters in the 434 OR region, PR and PRM, were mapped, and the effects of 434 repressor and Cro on promoter activity were assessed ... operator mutations. The binding of Cro to a 434 operator was probed by an ethylation interference experiment which, together with other data, indicates that 434 Cro and repressor probably turn off transcription by ... RNA polymerase to promoter sequences. In general, the 434 and λ right operators are controlled in a ...

Tópico(s): Bacterial Genetics and Biotechnology

1993 - Elsevier BV | Journal of Molecular Biology