A B-cell population of human cord blood lymphocytes was exposed to the B95–8 strain of Epstein-Barr virus (EBV), and simultaneous observations of immunofluorescence, cellular morphology and autoradiography were carried out in each individual cell. It was evident that EBV-determined nuclear antigen (EBNA) synthesis occurred prior to blastogenic response and DNA synthesis. EBNA-positive cells could be observed as early as 12 h after infection and reached a maximum of 17% at 24 h, followed by a plateau ...
Tópico(s): Immune Cell Function and Interaction
1979 - Karger Publishers | Intervirology
Kenzo Takada, Kohtaro Yamamoto, Toyoro Õsato,
Leukemic lymphocytes with B-cell markers were obtained from the peripheral blood of 4 patients with chronic lymphocytic leukemia (CLL) or acute lymphocytic leukemia (ALL); a significant number of T cells was not involved. The lymphocytes were inoculated with the transforming B95-8 Epstein-Barr virus (EBV). Synthesis of EBV-determined nuclear antigen (EBNA) occurred in three CLL preparations: 15.1--25.4% of cells expressed maximum EBNA 24--48 h after viral exposure. A characteristic immunofluorescence ...
Tópico(s): Parvovirus B19 Infection Studies
1980 - Karger Publishers | Intervirology
Kenzo Takada, Norio Shimizu, Akiko Tanabe-Tochikura, Yasuyuki Kuroiwa,
Epstein-Barr virus (EBV) is detected in several human cancers, such as Burkitt's lymphoma (BL), nasopharyngeal carcinoma, gastric cancer, and peripheral T-cell lymphoma. However, the role of EBV in the development of these cancers is still controversial. During cultivation of the EBV-positive BL line Akata, we found that EBV DNA is lost from some of the cells. Isolation of EBV-positive and -negative cell clones with the same origin made it possible to examine the effects of EBV in BL cells. The results ...
Tópico(s): Eosinophilic Disorders and Syndromes
1995 - Karger Publishers | Intervirology
On the basis of the sequential early events induced by Epstein-Barr virus (EBV), the infectability of human lymphocytes to EBV was quantified as a plateau value of the maximum nuclear antigen (EBNA) synthesis preceding cellular DNA synthesis. The frequency of EBV-infectable cells was an average of 7.6% of unfractionated umbilical cord lymphocytes and was 4.9% in the counterparts from human adults. In the B-cell-enriched populations, the average plateau values were 29.2% in umbilical cord and 15.5% ...
Tópico(s): T-cell and Retrovirus Studies
1982 - Karger Publishers | Intervirology
Yukio Iwamura, Hiroshi Hamada, Masami Muramatsu, Kenzo KATO,
Guinea pig (strain No 13) kidney cells were transformed with SV40 (SVNol3). When this transformant was injected subcutaneously into syngeneic irradiated guinea pigs, tumors were produced in some animals. One of them became transplantable (STB). Clones were obtained from cultured tumor cells (STC13). About one copy of the viral genome appeared to exist in both SVNol3 and STC13 clones as determined by reassociation kinetics. We have examined the distribution of integrated viral sequences in the host DNA ...
Tópico(s): CRISPR and Genetic Engineering
1984 - Karger Publishers | Intervirology
Y. Iwamura, K Mitamura, Kazuhiro Yanagi, Tetsuo Hashimoto, Kenzo KATO,
DNA of the human PLC/PRF/5 hepatoma cell line can induce the appearance of colonies on soft agar after transfection of BK-BK cells (BALB/c mouse kidney cells partially transformed by BK virus). Blot hybridization analyses indicate that the transformants contained the hepatitis B virus DNA sequence. This sequence disappeared during serial passage of transformants. The amplification and rearrangement of the integrated BK virus genome appears to be specifically associated with transformation.
Tópico(s): Parvovirus B19 Infection Studies
1986 - Karger Publishers | Intervirology
Nuclear antigen (EBNA) inducibility by Epstein-Barr virus (EBV) was assessed in relation to the presence of complement (C3) receptors and surface immunoglobulins (SIg) on leukemic lymphocytes. When erythrocyte-antibody-complement complex(EAC)-positive, SIg-negative B cells were exposed to EBV, EBNA synthesis occurred with a high frequency. In contrast, no significant EBNA induction was evident in SIg-positive, EAC-negative B cells. Cells possessing both markers were EBV-infectable, in proportion to ...
Tópico(s): Parvovirus B19 Infection Studies
1981 - Karger Publishers | Intervirology
Elke Beyer‐Finkler, F. Girardi, Martin Sillem, Herbert Pfister, Gen Kondoh, Qin Li, Jing Pan, Akira Hakura, Kazuo Tajima, Luis Cartier, Tadahito Kanda, H Teshima, Katsuyoshi Katase, Satoshi Umezawa, Hirohumi Watabe, Hiroyuki Takahashi, Takashi Onda, Kunito Yoshiike, K. Katase, Hideki Teshima, Y. Hirai, K. Hasumi, Martin Lewis, Paul J. Farrell, M. Kawanishi, Kenzo Takada, Norio Shimizu, Akiko Tanabe-Tochikura, Yasuyuki Kuroiwa, Masahiro Fujii, Haruo Tsuchiya, Xiaobo Meng, Motoharu Seiki, Iris Velhagen, Christiane Hilger, Claudia Lamberts, Hanswalter Zentgraf, Clans H. Schroder, Kazuhiko Koike, Thomas London, Alison A. Evans, Katherine A. McGlynn, Kenneth H. Buetow, Ping An, Long-Long Gao, Edward D. Lustbader, Eric A. Ross, Gong-chao Chen, Fu-min Shen, Kunitada Shimotohno, Kazunori Kajino, Hiroshi Aoki, Okio Hino, Takashi Yoshiki, Ohio Hino, Stephan Schaefer, Wolfram H. Gerlich, David Horner,
1995 - Karger Publishers | Intervirology
Elke Beyer‐Finkler, F. Girardi, Martin Sillem, Henry D. Pfister, Gen Kondoh, Qin Li, Pan Jing, Akira Hakura, Kazuo Tajima, Luis Cartier, Tadahito Kanda, H Teshima, Katsuyoshi Katase, Satoshi Umezawa, Hirohumi Watabe, Hiroyuki Takahashi, Takashi Onda, Kunito Yoshiike, K. Katase, H Teshima, Yoshimitsu Hirai, K Hasumi, Matthew A. Lewis, Paul J. Farrell, M. Kawanishi, Kenzo Takada, Norio Shimizu, Akiko Tanabe-Tochikura, Yasuyuki Kuroiwa, Masahiro Fujii, Haruo Tsuchiya, Xiaobo Meng, Motoharu Seiki, Iris Velhagen, Christiane Hilger, Claudia Lamberts, Hanswalter Zentgraf, Clans H. Schroder, Kazuhiko Koike, Thomas London, Alison A. Evans, Katherine A. McGlynn, Kenneth H. Buetow, Ping An, Long-Long Gao, Edward D. Lustbader, Eric D. Ross, Gong-chao Chen, Fu-min Shen, Kunitada Shimotohno, Kazunori Kajino, Hiroshi Aoki, Okio Hino, Takashi Yoshiki, Ohio Hino, Stephan Schaefer, Wolfram H. Gerlich, D. Horner,
1995 - Karger Publishers | Intervirology
T. W. Tinsley, F. O. MacCallum, James S. Robertson, Fred Brown, Narayan C. Khan, Blaine Hollinger, Joseph L. Melnick, Yukio Iwamura, Hiroshi Hamada, Masami Muramatsu, Kenzo Kato, Albrecht Gröner, Robert R. Granados, J.P. Burand, Marilyn A. Armstrong, Kenneth Fraser, P. V. Shirodaria, Stanley S. Lefkowitz, Victor E. Reyes Luna, Matthew S. Turner, William O. Dawson, Peter Bachmann, Werner Eichhorn,
1984 - Karger Publishers | Intervirology
T. W. Tinsley, F. O. MacCallum, James S. Robertson, Fred Brown, Narayan C. Khan, Blaine Hollinger, Joseph L. Melnick, Yukio Iwamura, Hiroshi Hamada, Masami Muramatsu, Kenzo Kato, Albrecht Gröner, Robert R. Granados, J.P. Burand, Marilyn A. Armstrong, Kenneth Fraser, P. V. Shirodaria, Stanley S. Lefkowitz, Victor E. Reyes Luna, Matthew S. Turner, William O. Dawson, Peter Bachmann, Werner Eichhorn,
Actinomycin-D-sensitive function, specificity in RNA plant viruses 224-228 Acyclovir, effect on cytomegalovirus infection in gui nea pigs 141-149 Antiphytoviral compound, inhibition of potato virus X 134-140 Aulographa califomica nuclear polyhedrosis virus, in teraction with nonpermissive cell lines 203-209 Avian -acute leukemia virus, transformation of nonpro ducer cell lines 121-133 -myeloblastosis virus oncogene region, candidate products 17-24 5-Azadihydrouracil, inhibition of potato virus X 134- ...
1984 - Karger Publishers | Intervirology