Optimization and Validation of an ELISA to Measure Specific Guinea Pig IgG1 Antibody as an Alternative to the in Vivo Passive Cutaneous Anaphylaxis Assay. Kawabata, T. T., Babcock, L. S., Gauggel, D. L., Asquith, T. N., Fletcher, E. R., Horn, P. A., Ratajczak, H. V., and Graziano, F. M. (1995). Fundam. Appl. Toxicol. 24, 238-246. Assessment of the allergenic potency of enzymes involves the use of a guinea pig model in which specific IgG1 antibody titers are used as the endpoint. The in vivo passive ...
Tópico(s): Allergic Rhinitis and Sensitization
1995 - Academic Press | Fundamental and Applied Toxicology
Immunotoxicology of Regional Lymphoid Tissue: The Respiratory and Gastrointestinal Tracts and Skin. Kawabata, T. T., Burleson, G. R., Ernst, P. B., and Ullrich, S. E. (1995). Fundam. Appl. Toxicol. 26, 8-19. The area of immunotoxicology has grown significantly over the past decade. Most of the research on methods development and validation and mechanisms of action have focused on the systemic immune system (i.e., spleen, thymus). However, since the regional/mucosal immune system plays an important ...
Tópico(s): Animal testing and alternatives
1995 - Academic Press | Fundamental and Applied Toxicology
The primary objective of this study was to examine the feasibility of using a mouse model to evaluate the immunogenicity of proteins as a potential method to determine occupational exposure guidelines. Mice were intratracheally administered a benchmark protein allergen, subtilisin Carlsberg (Alcalase) in detergent matrix once a week for 4 to 6 weeks and specific IgE and IgG1 levels were determined. In all experiments, specific IgE levels were determined by using a rat basophilic leukemia cell (RBL) ...
Tópico(s): Asthma and respiratory diseases
1996 - Academic Press | Fundamental and Applied Toxicology
Brian A. Fox, Vivien C. Yee, Lars C. Pedersen, Isolde Le Trong, Paul D. Bishop, Ronald E. Stenkamp, David C. Teller,
... 2.4963.4Glu-490OE13.4663.22.38 3.6832.4Glu-490OE23.6365.42.2928.0Wat-3006VO2.2136. ... Yu C. Nagao M. Sasaki R. Furuyoshi S. Kawabata N. Arch. Biochem. Biophys. 1995; 318: 307-313Crossref ... Yu C. Nagao M. Sasaki R. Furuyoshi S. Kawabata N. Arch. Biochem. Biophys. 1995; 318: 307-313Crossref ...
Tópico(s): Iron Metabolism and Disorders
1999 - Elsevier BV | Journal of Biological Chemistry
... with Benzo[a]pyrene or Cyclophosphamide. Temple, L., Kawabata, T. T., Munson, A. E., and White, K. ...
Tópico(s): Molecular Biology Techniques and Applications
1993 - Academic Press | Fundamental and Applied Toxicology
The gut mucosal immune system may be a primary target for many ingested chemicals. Methods have been developed to examine the effects of chemicals on the systemic humoral immune response; however, studies to evaluate various methods of assessing the local gut mucosal immune response in a toxicology assay have been limited. The objectives of this study were to examine the effects of the known immunosuppressive compound, cyclosporine (CYS), on the generation of a cholera toxin (CT)-specific gut mucosal ...
Tópico(s): T-cell and B-cell Immunology
1992 - Academic Press | Fundamental and Applied Toxicology
Francis Dalisay, Scott K. Okamoto, Jane Teneza, Christina Dalton, Kayli Lizama, Pallav Pokhrel, Yoshito Kawabata,
The present study examined types of scenarios in which Guam youths are offered tobacco—namely, combustible cigarettes and e-cigarettes—and betel (areca) nut. We conducted 10 focus groups with public middle school students (n = 34) from Guam. Results suggested that the types of offer scenarios of combustible cigarettes, e-cigarettes, and betel nut referenced by the students fall into two categories—direct-relational offers and indirect-contextual offers. The results also suggested that both categories ...
Tópico(s): Oral Health Pathology and Treatment
2023 - Multidisciplinary Digital Publishing Institute | International Journal of Environmental Research and Public Health