Sai Rashmika Velugula‐Yellela, Abasha Williams, Nicholas Trunfio, Chih‐Jung Hsu, Brittany Chavez, Seongkyu Yoon, Cyrus Agarabi,
Monoclonal antibody production in commercial scale cell culture bioprocessing requires a thorough understanding of the engineering process and components used throughout manufacturing. It is important to identify high impact components early on during the lifecycle of a biotechnology‐derived product. While cell culture media selection is of obvious importance to the health and productivity of mammalian bioreactor operations, other components such as antifoam selection can also play an important ...
Tópico(s): 3D Printing in Biomedical Research
2017 - American Chemical Society | Biotechnology Progress
Abasha Williams, Erik K. Read, Cyrus Agarabi, Scott Lute, Kurt Brorson,
Monoclonal antibodies are mainly produced by mammalian cell culture, which due to its complexity, results in a wide range of product variants/isoforms. With the growing implementation of Quality by Design (QbD) and Process Analytical Technology (PAT) in drug manufacturing, monitoring and controlling quality attributes within a predefined range during manufacturing may provide added consistency to product quality. To implement these concepts, more robust analytical tools could reduce the time needed ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2017 - Elsevier BV | Journal of Chromatography B
Abasha Williams, Teruo Hayashi, Daniel Wolozny, Bojiao Yin, Tzu-Chieh Su, Michael J. Betenbaugh, Tsung‐Ping Su,
Bcl-2 family proteins are known to competitively regulate Ca2+; however, the specific inter-organelle signaling pathways and related cellular functions are not fully elucidated. In this study, a portion of Bcl-xL was detected at the ER-mitochondrion interface or MAM (mitochondria-associated ER membrane) in association with type 3 inositol 1,4,5-trisphosphate receptors (IP3R3); an association facilitated by the BH4 and transmembrane domains of Bcl-xL. Moreover, increasing Bcl-xL expression enhanced ...
Tópico(s): ATP Synthase and ATPases Research
2016 - Springer Science+Business Media | Journal of Bioenergetics and Biomembranes
... Georgian SSR in the early 1970s (notably in Abasha Rayon), the RAPO system of administration has been ...
Tópico(s): Agricultural Development and Policies
1986 - Taylor & Francis | Soviet Geography
Hosea Kato Mande, Akeem Olawale Olaniyi, Mercy Richard, Bulus Sule, Deborah Abasha,
Vegetation cover in Kafanchan has been on the decrease which has suffered more and undue depletion and degradation. This research assessed the change of land use/land cover for the span of 45 years (1988-2023). This study made use of LANDSAT 2, 4, 8 and 8 images of the year 1988, 1998, 2016 and 2023 accordingly. The images were processed using Arcmap 10.3. For the secondary data collection, the area was demarcated into bare land, water bodies, vegetation and built-up areas. The results shows that ...
Tópico(s): Remote Sensing in Agriculture
2023 - | FUDMA Journal of Sciences
Chih‐Jung Hsu, Harsh V. Jain, Abasha Williams, Julie Wang, Scott Lute, Serge L. Beaucage, Kurt Brorson,
Abstract A rapid and cost‐effective transient transfection method for mammalian cells is essential for screening biopharmaceuticals in early stages of development. A library of 25 amphipathic trans‐acting oligodeoxythymidine phosphorothioate triester (dTtaPS) transfection reagents, carrying positively charged and lipophilic groups, has been constructed for this purpose. High‐throughput screening of the library, using an imaging cytometer and an automated microbioreactor system, has led to the identification ...
Tópico(s): RNA Interference and Gene Delivery
2017 - Wiley | Biotechnology and Applied Biochemistry
Abasha Williams, K Read Erik, D Agarabi Cyrus, L. B. Scott, A Brorson Kurt,
2017 - Elsevier BV | Journal of Chromatography B