Sunetra Roy, Abinadabe J de Melo, Yao‐Zhong Xu, Satish K. Tadi, Aurélie Négrel, Eric A. Hendrickson, Mauro Modesti, Katheryn Meek,
The classic nonhomologous end-joining (c-NHEJ) pathway is largely responsible for repairing double-strand breaks (DSBs) in mammalian cells. XLF stimulates the XRCC4/DNA ligase IV complex by an unknown mechanism. XLF interacts with XRCC4 to form filaments of alternating XRCC4 and XLF dimers that bridge DNA ends in vitro, providing a mechanism by which XLF might stimulate ligation. Here, we characterize two XLF mutants that do not interact with XRCC4 and cannot form filaments or bridge DNA in vitro. ...
Tópico(s): CRISPR and Genetic Engineering
2015 - Taylor & Francis | Molecular and Cellular Biology
Ineke Brouwer, Gerrit Sitters, Andrea Candelli, Stephanie J. Heerema, Iddo Heller, Abinadabe J. Melo de, Hongshan Zhang, Davide Normanno, Mauro Modesti, Erwin J.G. Peterman, Gijs J. L. Wuite,
Tópico(s): DNA and Nucleic Acid Chemistry
2016 - Nature Portfolio | Nature
Davide Normanno, Aurélie Négrel, Abinadabe J de Melo, S. Betzi, Katheryn Meek, Mauro Modesti,
XRCC4 and DNA Ligase 4 (LIG4) form a tight complex that provides DNA ligase activity for classical non-homologous end joining (the predominant DNA double-strand break repair pathway in higher eukaryotes) and is stimulated by XLF. Independently of LIG4, XLF also associates with XRCC4 to form filaments that bridge DNA. These XRCC4/XLF complexes rapidly load and connect broken DNA, thereby stimulating intermolecular ligation. XRCC4 and XLF both include disordered C-terminal tails that are functionally ...
Tópico(s): Cancer therapeutics and mechanisms
2017 - eLife Sciences Publications Ltd | eLife
Noah J. Goff, Manon Brenière, Christopher J. Buehl, Abinadabe J de Melo, Hana Hušková, Takashi Ochi, Tom L. Blundell, Weifeng Mao, Kefei Yu, Mauro Modesti, Katheryn Meek,
Abstract DNA double strand breaks (DSBs) are induced by external genotoxic agents (ionizing radiation or genotoxins) or by internal processes (recombination intermediates in lymphocytes or by replication errors). The DNA ends induced by these genotoxic processes are often not ligatable, requiring potentially mutagenic end-processing to render ends compatible for ligation by non-homologous end-joining (NHEJ). Using single molecule approaches, Loparo et al. propose that NHEJ fidelity can be maintained ...
Tópico(s): Carcinogens and Genotoxicity Assessment
2022 - Oxford University Press | Nucleic Acids Research
Produção Nacional
Revisado por pares
Jonatas Abinadabe Oliveira Silva, Fernando José da Silva, Lucia Oliveira de Macedo, Cléber Vinícius Brito dos Santos, Lêucio Câmara Alves, Rafael Antonio Nascimento Ramos, Maria Aparecida da Glória Faustino, Gílcia Aparecida de Carvalho,
Phlebotomines of the genus Lutzomyia are important vector for species of Leishmania, the etiological agent of leishmaniasis. The aim of this study was to assess the diversity of sandflies in an endemic area for Visceral Leishmaniasis (VL). Sampling was performed in four localities on the coast of the municipality of Goiana, state of Pernambuco, Northeastern Brazil. Specimens were collected monthly on three consecutive nights, from November 2017 to October 2018. CDC light traps were installed at a ...
Tópico(s): Research on Leishmaniasis Studies
2019 - Colégio Brasileiro de Parasitologia Veterinaria | Revista Brasileira de Parasitologia Veterinária/Brazilian Journal of Veterinary Parasitology
Ineke Brouwer, Gerrit Sitters, Andrea Candelli, Stephanie J. Heerema, Iddo Heller, Abinadabe J. Melo de, Hongshan Zhang, Davide Normanno, Mauro Modesti, Erwin J.G. Peterman, Gijs J. L. Wuite,
Nature 535, 566–569 (2016); doi:10.1038/nature18643 The Acknowledgements of this Letter should have included the following sentence: "The research leading to these results received funding from LASERLAB-EUROPE (grant agreement no. 284464, the European Commission's Seventh Framework Programme)." Thishas been corrected online.
Tópico(s): DNA and Nucleic Acid Chemistry
2017 - Nature Portfolio | Nature
Jonatas Abinadabe Oliveira Silva, Gílcia Aparecida de Carvalho, Maria Aparecida da Glória Faustino,
Tópico(s): Male Reproductive Health Studies
2017 - Editora MV Valero | PubVet
Jonatas Abinadabe Oliveira Silva, Paulo Affonso Bezerra De Almeida Galeão, Eliane Maria Ribeiro de Vasconcelos, Eloine Nascimento de Alencar,
ABSTRACTObjective: to evaluate the knowledge about toxoplasmosis along with medical and nursing students. Method: this is about an exploratory and descriptive study, from quantitative approach. Population’s study was composed of academics from the Nursing and Medicine courses, of the Federal University of Pernambuco and of the University of Pernambuco, after meeting inclusion criteria. Data collection was conducted form March to April 2010, using a questionnaire after approval by the Ethics in Research ...
Tópico(s): HIV/AIDS oral health manifestations
2011 - | Revista de Enfermagem UFPE on line
Produção Nacional
Revisado por pares
Jonatas Abinadabe Oliveira Silva, Elaine Rossi Ribeiro,
The presence of insects and mice in the hospital environment offer risks to patients and health professionals, as they can transmit diseases. The present work aims emphasize the importance of controlling pests and disease vectors in hospital settings, identifying the most common pests and vectors, the measures of prevention and control and expose sanitary legislation. Was performed an exploratory research, literature and documents on the theme. After collecting data, we describe some aspects of ...
Tópico(s): Food Safety and Hygiene
2015 - Editora MV Valero | PubVet
Produção Nacional
Abinadabe S. Andrade, E.R.C. da Silva,
This paper deals with a system composed of a three-level Neutral-Point- Clamped inverter fed by photovoltaic panels. The system is able to operate adequately even under partial shading operation by controlling the dc-link neutral point injected current. System model, pulse width modulation strategy, maximum power point tracking, and control logic for balancing the dc-link capacitors voltages are presented. Analysis of harmonic distortion, thermal stress, limits of control and power losses are included. ...
Tópico(s): Advanced DC-DC Converters
2016 - | Eletrônica de Potência
Produção Nacional
Revisado por pares
Jonatas Abinadabe Oliveira Silva, Gílcia Aparecida de Carvalho, Maria Aparecida da Glória Faustino,
The Visceral leishmaniasis (LV) is widely distributed in the territory of Pernambuco, although data on the dynamics and epidemiological characteristics of the disease in some areas are still fragmented and incipient; this is the case of the municipality of Goiana, where cases of visceral leishmaniasis in dogs and in humans have been recorded. The present study aims to analyze the behavior of the disease in the municipality of Goiana – PE in the period from 2007 to 2015 through the use of epidemiological ...
Tópico(s): Research on Leishmaniasis Studies
2017 - Editora MV Valero | PubVet
Produção Nacional
Abinadabe S. Andrade, Diego A. Acevedo-Bueno, E.R.C. da Silva,
This paper presents a capacitor voltage balancing method for the five-level diode-clamped inverter using level-shifted pulse-width modulation (LS-PWM), including the voltage balancing of DC-link capacitors. The proposed system has a reduced number of components for mitigation of losses. The DC-Link capacitor voltage is regulated by adjusting the switching duty cycles of DC/DC converter. Simulation results are presented to verify the validity of this method.
Tópico(s): Induction Heating and Inverter Technology
2018 - | Congresso Brasileiro de Automática
Produção Nacional
Raineesh Mishra, Abinadabe dos Santos Pires Soares, Abdullah Jassim Al-Qattan,
Background: The objective of this research is to provide an overview of the physical and biomechanical properties of composite barrier meshes frequently used in Laparoscopic Ventral Hernia Repair as well as reviewing the existing relevant literature assessing the characteristics and effectiveness of both procedures.Methods: A secondary research is used using existing literature review of the preclinical and clinical literature designed to compare the Composite prostheses with permanent barriers ( ...
Tópico(s): Minimally Invasive Surgical Techniques
2019 - Egypts Presidential Specialized Council for Education and Scientific Research | The Egyptian Journal of Hospital Medicine