Wenwen Wu, Wei Xi, Huimin Li, Mengxiang Yang, Xialei Yao,
... diverse human cancers. However, the role of circ‑ACACA in NSCLC progression remains elusive. Quantitative polymerase chain ... conducted to detect the expression levels of circ‑ACACA and microRNA (miR)‑1183 in NSCLC tissues and ... measured by western blotting. The interaction between circ‑ACACA and miR‑1183 was predicted by circular RNA ... established to investigate the biological roles of circ‑ACACA in vivo. The level of circ‑ACACA was markedly upregulated in NSCLC tissues and cells, ... the expression of miR‑1183. Knockdown of circ‑ACACA inhibited proliferation and migration of NSCLC cells and ...
Tópico(s): MicroRNA in disease regulation
2020 - Spandidos Publishing | International Journal of Molecular Medicine
Hui Zhang, Shaoyou Liu, Zhouda Cai, Weimin Dong, Jian‐Heng Ye, Zhiduan Cai, Zhaodong Han, Yuxiang Liang, Yangjia Zhuo, Yong Luo, Xuejin Zhu, Yulin Deng, Yuting Zhang, Ren Liu, Yuanfa Feng, Jiarun Lai, Rui Zhou, Huijing Tan, Weide Zhong,
Background and aim: Silencing the expression of ACACA inhibits cell proliferation and induces apoptosis in prostate cancer LNCaP cells.However, the role of ACACA in other prostate cancer cells is not fully understood.Also, the effect of knocking down ACACA gene on mitochondria remains unclear.This study aimed to discover the specific role of ACACA gene in prostate cancer (PCa) DU145 and PC3 ... effects on mitochondrial potential.Methods: The expression of ACACA gene was detected in human prostate cancer tissue ...
Tópico(s): Metabolomics and Mass Spectrometry Studies
2020 - Ivyspring International Publisher | Journal of Cancer
... the role of circ‑acetyl‑CoA carboxylase α (ACACA) in the progression of cervical cancer (CC). The expression levels of circ‑ACACA in several CC cell lines were first determined using reverse transcription‑quantitative PCR. circ‑ACACA expression was subsequently knocked down to evaluate its ... using western blotting. The binding interactions among circ‑ACACA, microRNA (miR)‑582‑5p and ERO1A were validated ... determine the potential underlying mechanism by which circ‑ACACA affected CC cell functions. The results revealed that ...
Tópico(s): Cancer, Lipids, and Metabolism
2021 - Spandidos Publishing | Oncology Letters
Shaoyou Liu, Jiarun Lai, Yuanfa Feng, Yangjia Zhuo, Hui Zhang, Yupeng Chen, Jinchuang Li, Xinyue Mei, Yanting Zeng, Jiaming Su, Yulin Deng, Funeng Jiang, Sheng-Bang Yang, Huijing Tan, Chi Tin Hon, Wei Sun, Zhaodong Han, Fen Wang, Weide Zhong,
... that the expression of acetyl-CoA carboxylase alpha (ACACA), which encodes acetyl-CoA carboxylase 1 (ACC1), was ... in human PCa. In addition, patients with high ACACA expression had a short disease-free survival time. We also reported that depletion of ACACA reduced de novo fatty acid synthesis and PI3K/ ... cell lines DU145 and PC3. Furthermore, depletion of ACACA downregulates mitochondrial beta-oxidation, resulting in mitochondrial dysfunction, ... exacerbated both shRNA depletion and pharmacological inhibition of ACACA-induced apoptosis in vitro. Collectively, our results suggest ...
Tópico(s): Metabolomics and Mass Spectrometry Studies
2022 - Elsevier BV | Journal of Biological Chemistry
Young-Sil Yoon, Woo-Young Seo, Minwoo Lee, Seong‐Tae Kim, Seung‐Hoi Koo,
... number of enzyme genes, such as Fasn or Acaca, that are involved in this pathway at the ... number of enzyme genes, such as Fasn or Acaca, that are involved in this pathway at the ... genes, such as Fasn (fatty acid synthase) or Acaca (acetyl-CoA carboxylase α). The control of lipogenic ... Expression of lipogenic genes, such as Fasn or Acaca, is elevated in liver-specific SREBP-1c transgenic ... genes involved in the lipogenesis, such as Fasn, Acaca, and Gpd1 (glycerol-3-phosphate dehydrogenase 1) are ... RNAi versus US Srebf1 0.571 1.347 Acaca 0.991 1.764 Fasn 0.652 1. ...
Tópico(s): Liver Disease Diagnosis and Treatment
2009 - Elsevier BV | Journal of Biological Chemistry
Maureen T. Travers, Marie Cambot, Helen T. Kennedy, Gilbert Lenoir, MICHAEL C. BARBER, V. Joulin,
The mammalian gene (ACACA) encoding acetyl-CoA carboxylase-α, a key regulatory enzyme of fatty acid synthesis, is transcribed from multiple promoters. We have delineated the 5′ boundary of ACACA in four species (human, mouse, rat, and ovine). The 5′ end of ACACA is located within a 600- to 700-bp ... and rat, this promoter, now referred to as Acaca PI, is located 43 kb upstream of the ... The shared promoter coregulates transcripts for TADA2L and ACACA in an asymmetric fashion in human and mouse ... and >200-fold greater abundance of Tada2l and Acaca PI transcripts, respectively, in brain. Thus, regulation of ...
Tópico(s): Peroxisome Proliferator-Activated Receptors
2004 - Elsevier BV | Genomics
Monika Stachowiak, Joanna Nowacka‐Woszuk, M. Szydłowski, M. Świtoński,
... there are genes encoding acetyl-CoA carboxylase alpha (ACACA) and sterol regulatory element binding transcription factor 1 ( ... 5 known and 2 novel SNPs in the ACACA gene and 2 known and 6 novel polymorphisms ... SNPs segregated as 6 and 10 haplotypes in ACACA and SREBF1, respectively. Two SNPs in ACACA were associated with fatness and performance traits in ... differences in the transcript level were observed for ACACA only. No correlation between the transcript levels and ... fat tissues. We concluded that polymorphisms of the ACACA and SREBF1 genes are promising markers for pig ...
Tópico(s): Peroxisome Proliferator-Activated Receptors
2013 - Elsevier BV | Meat Science
Monika Stachowiak, Krzysztof Flisikowski,
... to investigate the allele proportions of candidate genes (ACACA, ADIPOR1, FASN, LEP, ME1, SCD, and TNF) involved ... Pietrain breeds. We detected differential allelic expression of ACACA, LEP, SCD, and TNF in all tissues analyzed. ... CpG islands located in 5′-flanking regions of ACACA and SCD. Comparison between samples showing markedly disproportionate ... an SNP located in the 3′UTR of ACACA with its allelic expression in Polish Landrace pigs, ... are necessary to identify cis-regulatory elements affecting ACACA, LEP, SCD, and TNF expression in porcine fat ...
Tópico(s): Adipose Tissue and Metabolism
2019 - Springer Nature | Journal of Applied Genetics
Yung‐Chi Huang, Ming‐Feng Hou, Ying‐Ming Tsai, Yi‐Chung Pan, Pei-Hsun Tsai, Yi-Shiuan Lin, Chao‐Yuan Chang, Eing‐Mei Tsai, Ya‐Ling Hsu,
... PyVT mice before cancer spreading. The expression of ACACA (acetyl-CoA carboxylase α) was downregulated in the ... the synthesis of fatty acid. The downregulation of ACACA in lung fibroblasts triggered a senescent and inflammatory ... of CXCL1 in the lungs. Knock-in of ACACA prevented lung metastasis in the MMTV-PyVT mouse model, further supporting that ACACA was involved in the remodeling of the lung ... together, these data revealed a mechanism by which ACACA downregulation directed the formation of an immunosuppressive lung ...
Tópico(s): Epigenetics and DNA Methylation
2023 - Springer Nature | Cellular Oncology
Marta García-Fernández, Beatriz Gutiérrez‐Gil, E. García‐Gámez, J. J. Arranz,
Acetyl-CoA carboxylase (ACACA) is the rate-limiting enzyme in the biosynthesis of palmitic acid and long-chain fatty acids. The dietary intake ... in assessing the possible influence of the ovine ACACA gene on milk fatty acid composition and its ... investigation into the genetic variability of the ovine ACACA gene. We sequenced approximately 6.6 kb of ovine ACACA cDNA, including most of the coding sequence of ... other mutations showing a functional effect on the ACACA enzyme. Hence, the characterisations of the allelic variants ...
Tópico(s): Biotin and Related Studies
2010 - Elsevier BV | Small Ruminant Research
María Muñoz, Ana I. Fernández, Rita Benítez, Ramona N. Pena, J. M. Folch, Carmen García‐Rodríguez, L. Silió, Estefânia Alves,
... been characterized. Moreover, two putative promoters of porcine ACACA gene have also been investigated. New isoforms and ... and vaccenic fatty acids, maps close to the ACACA gene location. The most significant associations have been ... 53840T > C) and one synonymous (c.5634T > C) ACACA SNPs and these fatty acids. Complementary analyses including ACACA gene expression quantification and association studies in other ... do not support the expected causal effect of ACACA SNPs.
Tópico(s): Meat and Animal Product Quality
2013 - Taylor & Francis | Animal Biotechnology
Alejandro Vázquez‐Martín, Bruna Corominas-Faja, Sílvia Cufí, Luciano Vellón, Cristina Oliveras‐Ferraros, Octavio J. Menendez, Jorge Joven, Ruth Lupu, Javier A. Menéndez,
... overexpression and hyperactivity of the acetyl-CoA carboxylase (ACACA) and fatty acid synthase (FASN) lipogenic enzymes that ... β-F1-ATPase subunit. The pharmacological inhibition of ACACA and FASN activities markedly decreases reprogramming efficiency, and ACACA and FASN expression are notably upregulated in iPS ... IF1/β-F1-ATPase ratio and downregulate the ACACA/FASN lipogenic enzymes in iPS cells. The mitochondrial H+-ATP synthase and the ACACA/FASN-driven lipogenic switch are newly characterized as ...
Tópico(s): Cancer, Lipids, and Metabolism
2013 - Taylor & Francis | Cell Cycle
Monika Stachowiak, Krzysztof Flisikowski,
... to investigate the allele proportions of candidate genes (ACACA, ADIPOR1, FASN, LEP, ME1, SCD, and TNF) involved ... Pietrain breeds.We detected differential allelic expression of ACACA, LEP, SCD, and TNF in all tissues analyzed. ... CpG islands located in 5′-flanking regions of ACACA and SCD.Comparison between samples showing markedly disproportionate ... an SNP located in the 3′UTR of ACACA with its allelic expression in Polish Landrace pigs, ... are necessary to identify cis-regulatory elements affecting ACACA, LEP, SCD, and TNF expression in porcine fat ...
Tópico(s): Animal Genetics and Reproduction
2013 - Springer Nature | Journal of Applied Genetics
Marika Vitali, Corrado Dimauro, Rubina Sirri, Martina Zappaterra, Paolo Zambonelli, Elisabetta Manca, Dalal Sami, Domenico Pietro Lo Fiego, R. Davoli,
... 24 genes were differentially expressed among diets, namely ACACA, ADIPOQ, ADIPOR1, CHREBP (MLXPL), ELOVL6, FASN, G6PD, PLIN2, ... the univariate analysis displayed an increased expression of ACACA, ADIPOQ, ADIPOR1, CHREBP, ELOVL6, FASN, PLIN2, RXRA and SCD in D4 compared to D2. Similarly, ACACA, ADIPOQ, ADIPOR1, ELOVL6 and SCD were highly expressed ... D2 was not different from D3 and that ACACA, SCD and FASN expression made D4 different from ... and D1, the expression levels of ELOVL6 and ACACA were the most influenced. This research provides evidence ...
Tópico(s): Antioxidant Activity and Oxidative Stress
2018 - Public Library of Science | PLoS ONE
Jinghui Heng, Zhihui Wu, Min Tian, Jiaming Chen, Hanqing Song, Fang Chen, Wutai Guan, Shihai Zhang,
... group with the down-regulation of lipogenic genes (ACACA, FASN, PPAR-r, SREBP-1c in ventral and ... BCAA group with the decrease of lipogenic genes (ACACA in ventral, ACACA and FASN in dorsal fat, ACACA, FASN, SREBP-1c in liver) and the increase ... liver (P < 0.05). Decreased m6A modification of ACACA and FASN in ventral and dorsal adipose tissues ...
Tópico(s): Peroxisome Proliferator-Activated Receptors
2020 - BioMed Central | Nutrition & Metabolism
Kasturi Bhattacharjee, Moumita Nath, Yashmin Choudhury,
... in association with the expression levels of the ACACA and ACACB genes which encode the rate limiting ... of cancer depending on the expression of the ACACA and ACACB genes. In addition, related articles were ... the altered expression of the genes. Overexpression of ACACA has been reported in cancers of the breast, ... retinoblastoma, thyroid and melanoma. Increased expression of both ACACA and ACACB has been reported in hepatocellular carcinoma ...
Tópico(s): Peroxisome Proliferator-Activated Receptors
2020 - Elsevier BV | Meta Gene
David Gallardo, Raquel Quintanilla, L. Varona, Isabel Dı́az, Óscar Ramírez, Ramona N. Pena, Marcel Amills,
Acetyl-coenzyme A carboxylase alpha (ACACA) catalyses the first committed step in the biosynthesis of long-chain fatty acids (FA) by converting acetyl-CoA into malonyl-CoA. In pigs, the ACACA gene maps to a chromosome 12 QTL with ... have sequenced the coding region of the pig ACACA gene in 15 pigs, identifying 21 polymorphic sites ... mapping to the chromosomal region containing the pig ACACA gene, with marked effects on FA composition of ...
Tópico(s): Genetic and phenotypic traits in livestock
2009 - Wiley | Animal Genetics
Sung-Chul Shin, Jae-Pil Heo, Eui-Ryong Chung,
... by genetic factors.Acetyl-Coenzyme A carboxylase-α (ACACA) plays a key role in the regulation and ... acid biosynthesis in mammalian animals.The gene encoding ACACA enzyme was chosen as a candidate gene for ... effects of single nucleotide polymorphisms (SNPs) in the ACACA gene on beef carcass and meat traits in ... fragment of intron I region of the Hanwoo ACACA gene and identified two SNPs.Genotyping of the ... were formed with two SNP information within the ACACA gene.We constructed haplotypes using the HaploView software ...
Tópico(s): Molecular Biology Techniques and Applications
2011 - Asian-Australasian Association of Animal Production Societies | Asian-Australasian Journal of Animal Sciences
B. Moioli, Maria Carmela Scatà, Giovanna De Matteis, G. Annicchiarico, Gennaro Catillo, F. Napolitano,
... coding region of the acetyl‐CoA carboxylase alpha ( ACACA ) gene, which plays an important role in de ... genomic regions encoding the three promoters of the ACACA gene were directly sequenced in 264 sheep of ... mammary gland, the SNP in PIII of the ACACA gene might affect the variation of fat content ...
Tópico(s): Genetic Mapping and Diversity in Plants and Animals
2013 - Wiley | Animal Genetics
O. Urrutia, J.A. Mendizábal, K. Insausti, B. Soret, A. Purroy, Alejandro Toledo‐Arana,
... lipase (LPL), acetyl-CoA carboxylase 1 or alpha (ACACA), stearoyl-CoA desaturase (SCD), fatty acid desaturase 1 ( ... IM depots. In the SC fat, expression of ACACA and SCD was downregulated in L10 compared to ... mRNA levels. In the IM fat, downregulation of ACACA (L5 and L10), SCD (L10), FADS1 (L5 and ... FA formation at the transcriptional level by downregulating ACACA and SCD, respectively. Hence, linseed supplementation did not ...
Tópico(s): Fatty Acid Research and Health
2015 - Elsevier BV | Livestock Science
Xudong Sun, Yazhe Wang, Juan J. Loor, Ryan Bucktrout, Xin Shu, Hongdou Jia, Jihong Dong, Rankun Zuo, Guowen Liu, Xiaobing Li, Xinwei Li,
... fatty acid synthase (FASN), acetyl-coA carboxylase 1 (ACACA), butyrophilin (BTN1A1), and xanthine dehydrogenase (XDH) was greater ... mammary epithelial cells increased the abundance of FASN, ACACA, XDH, and BTN1A1, and increased triacylglycerol (TAG) content ... Exogenous NEFA increased the abundance of CIDEA, FASN, ACACA, XDH, and BTN1A1, and increased TAG content in ... knockdown of CIDEA reversed the upregulation of FASN, ACACA, XDH, and BTN1A1 abundance and TAG content induced ...
Tópico(s): Genetic and phenotypic traits in livestock
2018 - Elsevier BV | Journal of Dairy Science
Bingbing Zhang, Ming Li, Wei Yang, Juan J. Loor, Shuang Wang, Yingying Zhao, Han Guo, Xinru Ma, Cheng Xia, Chuang Xu,
... and GRP78) and lipogenic genes (FASN, SREBP1, and ACACA). Cellular mechanisms to establish links between BHB and ... h. The mRNA abundance of FASN, SREBP1, and ACACA and lipid droplet formation peaked at 1.2 ... h. Transcription and protein abundance of FASN, SREBP1, ACACA, and ER stress proteins returned to basal levels ... Ca2+) also increased abundance of ORAI1, FASN, SREBP1, ACACA, and ER stress proteins. Data suggest that the ...
Tópico(s): Ion Channels and Receptors
2020 - Elsevier BV | Journal of Dairy Science
Mailin Gan, Linyuan Shen, Yuan Fan, Ya Tan, Lin Liu, Lei Chen, Ye Zhao, Lili Niu, Guoqing Tang, Qiang Li, Xu Xu, Tinghuan Zhang, Xuewei Li, Shunhua Zhang, Li Zhu,
... luciferase reporter system indicated Acetyl-CoA carboxylase alpha (ACACA) as a target gene of ssc-miR-451. ... with intramuscular fat content (IMF) and positively correlated ACACA expression with IMF. Further analysis of fatty acid ... lipid deposition and fatty acid composition by targeting ACACA, and ssc-miR-451 may serve as a ...
Tópico(s): Molecular Biology Techniques and Applications
2020 - Multidisciplinary Digital Publishing Institute | Animals
Marzena M. Kęsek-Woźniak, Edyta Wojtas, Anna E. Zielak-Steciwko,
... relation between polymorphism of acetyl-CoA carboxylase α (ACACA), stearoyl-CoA desaturase 1 (SCD1), diacylglycerol acyltransferase 1 ( ... polymorphisms (SNPs): AJ312201.1g.1488C > G SNP in ACACA gene, A293V SNP in SCD1 gene, and K232A ... concentration of healthy FA in homozygotes: GG in ACACA, VV in SCD1, and KK in DGAT1 were ...
Tópico(s): Fatty Acid Research and Health
2020 - Multidisciplinary Digital Publishing Institute | Animals
Katarzyna Piórkowska, Martyna Małopolska, Katarzyna Ropka‐Molik, Magdalena Szyndler‐Nędza, Angelika Wiechniak, Kacper Żukowski, B.D. Lambert, M. Tyra,
... mutations within candidate genes for fat content (SCD, ACACA, and FASN) were selected, based on RNA-seq ... meat colour. In turn, a mutation in the ACACA gene showed a relationship with IMF content in ... populations due to the crucial role of SCD, ACACA, and FASN genes in lipid metabolism.
Tópico(s): Animal Nutrition and Physiology
2020 - Multidisciplinary Digital Publishing Institute | Animals
Xiaoting Lou, Xiyue Zhou, Haiyan Li, Xiangpeng Lu, Xinzhu Bao, Kaiqiang Yang, Xin Liao, Hanxiao Chen, Hezhi Fang, Yanling Yang, Jianxin Lyu, Hong Zheng,
... evaluated the possible disease-causing role of the ACACA gene in developmental delay and investigated the pathogenesis ... reviewed. Whole exome sequencing found two variants of ACACA with unknown significance. ACC1 mRNA expression level, protein ... We report an individual with biallelic mutations in ACACA, presenting global development delay. In vitro studies revealed ...
Tópico(s): Mitochondrial Function and Pathology
2021 - Frontiers Media | Frontiers in Cell and Developmental Biology
Antonio Gnoni, Benedetta Di Chiara Stanca, Laura Giannotti, Gabriele V. Gnoni, Luisa Siculella, Fabrizio Damiano,
... citrate carrier (CiC), cytosolic acetyl-CoA carboxylase 1 (ACACA), and diacylglycerol acyltransferase 2 (DGAT2) involved in fatty ... Que mainly occurs through a strong phosphorylation of ACACA, which catalyzes the committing step in the DNL pathway. The high level of ACACA phosphorylation in Que-treated cells was explained by ... inhibition of the DNL pathway by acting on ACACA/AMPK/PP2A axis; thus, suggesting this flavonoid as ...
Tópico(s): Endoplasmic Reticulum Stress and Disease
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Francisco J. Díaz, Alexander Meary, María J. Arranz, Gualberto Ruaño, Andreas Windemuth, José de León,
Acetyl-coenzyme A carboxylase alpha (ACACA) single-nucleotide polymorphism (SNP) (rs2229416) was significantly associated with hypertriglyceridemia, during exploration of antipsychotic direct effects on lipids. Neuropeptide Y (NPY) ... three (rs1266175, rs12453407 and rs9906543) of eight additional ACACA SNPs were significantly associated with hypertriglyceridemia in those ... of interest, but not in controls. Five other ACACA SNPs, three additional NPY SNPs, and seven additional ...
Tópico(s): Receptor Mechanisms and Signaling
2009 - Elsevier BV | Schizophrenia Research
Bouabid Badaoui, J. M. Serradilla, A. Tomás, B. Urrutia, J. Arés, J. Carrizosa, Armand Sánchez, J. Jordana, Marcel Amills,
Acetyl-coenzyme A carboxylase α (ACACA), the major regulatory enzyme of fatty acid biosynthesis, catalyzes the conversion of acetyl-CoA to malonyl-CoA. We have sequenced 5.5 kb of the goat ACACA cDNA in 18 individuals. The translated cDNA sequence ... AA identity (99%) with the ovine and bovine ACACA orthologous sequences. One silent single nucleotide polymorphism was ...
Tópico(s): Neurological diseases and metabolism
2007 - Elsevier BV | Journal of Dairy Science
Gloria Muñoz, E. Alves, Ana I. Fernández, C. Óvilo, C. Barragán, Jordi Estellé, Rodrigo A. Quintanilla, J. M. Folch, L. Silió, Maria Carmen Rodríguez, Ana I. Fernández,
... polypeptide (GIP) and acetyl-coenzyme A carboxylase alpha (ACACA) were tested as candidate genes for the second ... SNPs in GIP and 15 synonymous SNPs in ACACA cDNA sequences. Two missense SNPs in GIP showed ... significant associations were found for two SNPs in ACACA with stearic, palmitoleic and vaccenic fatty-acid concentrations. ...
Tópico(s): Genetic and phenotypic traits in livestock
2007 - Wiley | Animal Genetics