
Maria Isabel de Oliveira, Paul A. Rota, Suely Pires Curti, Cristina Adelaide Figueiredo, Ana Maria Sardinha Afonso, Márcia Theobaldo, Luiza T. M. de Souza, Stephanie Liffick, William J. Bellini, José Cássio de Moraes, Klaus E. Stevien, Edison Luíz Durigon,
During a resurgence of measles in São Paulo, Brazil, in 1997, >40,000 cases (peak incidence rate of 246/100,000 inhabitants) and 42 measles-related deaths were reported. Reverse transcriptase-polymerase chain reaction and nucleotide sequencing were used to analyze specimens from patients who had typical clinical measles infection during this outbreak and from six patients who had had measles in 1995 and 1996. Although wild-type measles viruses (genotypes D5 and D6) were present in São Paulo before ...
Tópico(s): Immunodeficiency and Autoimmune Disorders
2002 - Centers for Disease Control and Prevention | Emerging infectious diseases

C. A. Figueiredo, Giselle Burlamaqui Klautau, Ana Maria Sardinha Afonso, Silvana Beres Castrignano, Maria Isabel de Oliveira, Suely Pires Curti, G.G. Squarcina, Kazuyuki Narimatsu, Zied Rasslan, Carla Andrade Lima, Valdir Golin, E.F. Tadeo, F.J. Spagunolo, Berenice Oliveira Cataldo, Edison Luíz Durigon,
Rubella virus (RV) infection has sporadically been linked to Guillain-Barré syndrome (GBS), but the association with RV has been based only on clinical and/or serological backgrounds. In the present case it was possible to isolate RV (genotype 1a) from cerebrospinal fluid and peripheral blood mononuclear cells of an 18-year-old woman diagnosed with GBS after clinical manifestations of rubella. This report contributes to confirm RV as one of the triggering pathogens of this peripheral nervous system ...
Tópico(s): Long-Term Effects of COVID-19
2008 - Elsevier BV | Journal of Clinical Virology

José Cássio de Moraes, Cristiana M. Toscano, Eliana Nogueira Castro de Barros, Brigina Kemp, Fabio Lievano, Steven Jacobson, Ana Maria Sardinha Afonso, Peter M. Strebel, K. Lisa Cairns,
Background. Few population-based studies of infectious etiologies of fever-rash illnesses have been conducted. This study reports on enhanced febrile-rash illness surveillance in Campinas, Brazil, a setting of low measles and rubella virus transmission.
Tópico(s): Herpesvirus Infections and Treatments
2011 - Oxford University Press | The Journal of Infectious Diseases
Jodi B. Black, Edison Luíz Durigon, Kathleen Kite-Powell, Letícia Souza, S. P. Curli, Ana Maria Sardinha Afonso, Márcia Theobaldo, Philip E. Pellett,
We collected acute-phase and convalescent-phase serum samples from Brazilian patients who presented with exanthem of unknown origin and evaluated these samples by means of an immunoblot assay for seroconversion to human herpesvirus 6 (HIV-6) or human herpesvirus 7 (HIV-7). Measles or rubella had been clinically diagnosed in all these patients, but their sera were negative for antibodies to both measles virus and rubella virus. Twenty percent of the patients clearly seroconverted to HHV-6 after manifestation ...
Tópico(s): Herpesvirus Infections and Treatments
1996 - Oxford University Press | Clinical Infectious Diseases

Cláudio Mendes Pannuti, Ricardo José Morello, José Cássio de Moraes, Suely Pires Curti, Ana Maria Sardinha Afonso, Maria Cláudia Corrêa Camargo, Vanda Akico Ueda Fick de Souza,
ABSTRACT Despite almost universal use of measles vaccines in recent decades, epidemics of the disease continue to occur. Understanding the role of primary vaccine failure (failure to seroconvert after vaccination) and secondary vaccine failures (waning immunity after seroconversion) in measles epidemics is important for the evaluation of measles control programs in developing countries. After a measles epidemic in São Paulo, Brazil, 159 cases previously confirmed by detection of specific immunoglobulin ...
Tópico(s): Parvovirus B19 Infection Studies
2004 - American Society for Microbiology | Clinical and Vaccine Immunology

Patrícia Evelin Silva, Cristina Adelaide Figueiredo, Adriana Luchs, Terezinha Maria de Paiva, Margarete Aparecida Benega Pinho, Renato Sousa Paulino, Daniela Bernardes Borges da Silva, Maria Isabel de Oliveira, Ana Maria Sardinha Afonso, Maria Isabel de Oliveira,
The aims of this study were to investigate the human bocavirus (HBoV) frequency and genotypes in hospitalized children <5 years presenting acute respiratory infections (ARI) within the São Paulo metropolitan area. Nasopharyngeal samples from 300 patients, previously screened for common respiratory viruses, were tested by qPCR for the NSP1 and NP-1 genes. The VP1/2 gene in positive samples was then amplified by PCR and sequenced. A total of 49 positive HBoV cases (16.3%; mean Ct value of 34.41) were ...
Tópico(s): Viral gastroenteritis research and epidemiology
2018 - Springer Science+Business Media | Archives of Virology

Flávia Helena Ciccone, Telma Regina Marques Pinto Carvalhanas, Ana Maria Sardinha Afonso, Brendan Flannery, Eliseu Alves Waldman,
To review measles IgM-positive cases of febrile rash illnesses in the State of São Paulo, Brazil, over the five-year period following interruption of measles virus transmission.We reviewed 463 measles IgM-positive cases of febrile rash illness in the State of São Paulo, from 2000 to 2004. Individuals vaccinated against measles < or = 56 days prior to specimen collection were considered to be exposed to the vaccine. Serum from the acute and convalescent phases was tested for evidence of measles, rubella, ...
Tópico(s): Immunodeficiency and Autoimmune Disorders
2010 - Brazilian Society of Tropical Medicine | Revista da Sociedade Brasileira de Medicina Tropical

T. B. Hyde, H. Sato, Hao Li, Brendan Flannery, Qi Zheng, Kathleen Wannemuehler, Filomena Ciccone, H. de Sousa Marques, Lily Yin Weckx, Marco Aurélio Palazzi Sáfadi, Esther Moraes, Marisa M rcia Mussi Pinhata, Jaime Olbrich Neto, Maria C. Bevilacqua, A. T., T. A. Monteiro, C. A. Figueiredo, Jon Kim Andrus, Susan E. Reef, Cristiana M. Toscano, Carlos Castillo‐Solórzano, Joseph P. Icenogle, for the CRS Biomarker Study Group, T. B. Hyde, Susan E. Reef, Joseph P. Icenogle, Hao Li, Brendan Flannery, Qi Zheng, Kathleen Wannemuehler, Telma Regina Marques Pinto Carvalhanas, Filomena Ciccone, H. Sato, Cristiana M. Toscano, Jon Kim Andrus, Carlos Castillo‐Solórzano, H. de Sousa Marques, Hartwig Paulo, Lily Yin Weckx, Angela Rico de Souza, Marco Aurélio Palazzi Sáfadi, E Moraes, Marisa Márcia Mussi‐Pinhata, Fabiana Amaral, Jaime Olbrich Neto, Maria C. Bevilacqua, R. B. Amantini, A. T., T. A. Monteiro, R. F. Bento, Ana Maria Sardinha Afonso, C. A. Figueiredo, Suely Pires Curti,
Background. Congenital rubella syndrome (CRS) case identification is challenging in older children since laboratory markers of congenital rubella virus (RUBV) infection do not persist beyond age 12 months. Methods. We enrolled children with CRS born between 1998 and 2003 and compared their immune responses to RUBV with those of their mothers and a group of similarly aged children without CRS. Demographic data and sera were collected. Sera were tested for anti–RUBV immunoglobulin G (IgG), IgG avidity, ...
Tópico(s): Immunodeficiency and Autoimmune Disorders
2014 - Oxford University Press | The Journal of Infectious Diseases

C. A. Figueiredo, Maria Isabel de Oliveira, Ana Maria Sardinha Afonso, Suely Pires Curti, Edison Luíz Durigon,
Tópico(s): Rabies epidemiology and control
2010 - Springer Science+Business Media | Infection

Maria Isabel de Oliveira, Ana Maria Sardinha Afonso, Suely Pires Curti, Patrícia Evelin Silva, Tamyris Fernanda Barbosa, Elian Reis E Silva, Cristina Adelaide Figueiredo,
Summary Introduction: Virus surveillance strategies and genetic characterization of human parvovirus B19 (B19V) are important tools for regional and global control of viral outbreak. In São Paulo, Brazil, we performed a study of B19V by monitoring the spread of this virus, which is an infectious agent and could be mistakenly reported as a rash and other types of infection. Method: Serum samples were subjected to enzyme immunoassay, real time polymerase chain reaction, and sequencing. Results: From ...
Tópico(s): Dermatological and COVID-19 studies
2017 - Brazilian Medical Association | Revista da Associação Médica Brasileira

C. A. Figueiredo, Maria Isabel de Oliveira, Suely Pires Curti, Ana Maria Sardinha Afonso, Ailin Yu, Felipe Augusto Souza Gualberto, Edison Luíz Durigon,
Abstract Rubella virus (RV) is an important human pathogen that causes rubella, an acute contagious disease. It also causes severe birth defects collectively known as congenital rubella syndrome when infection occurs during the first trimester of pregnancy. Here, we present the phylogenetic analysis of RV that circulated in São Paulo during the 2007–2008 outbreak. Samples collected from patients diagnosed with rubella were isolated in cell culture and sequenced. RV RNA was obtained from samples or ...
Tópico(s): Syphilis Diagnosis and Treatment
2012 - Wiley | Journal of Medical Virology

Elmer Antonio Mamani-Calcina, Fernando José Gomes Landgraf, C.R.F. Azevedo,
... site of iron artifacts, the XVIII century) and Afonso Sardinha (São Paulo, Brazil production site of iron artifacts, ...
Tópico(s): Hydrogen embrittlement and corrosion behaviors in metals
2016 - Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímer | Materials Research
Joana Dias Coelho, Ana Maria Sardinha Afonso, Ana Barata Feio,
Lymphomatoid papulosis (LyP) is a rare skin lymphoproliferative disorder that has been reported only rarely in children. It is included in the World Health Organization classification of cutaneous lymphomas. We report a case of LyP in a 13-year-old Caucasian girl who presented with a 6-month history of recurrent papular lesions on the left upper arm. Histopathologic examination showed a diffuse cellular infiltration of small and medium-sized T lymphocytes CD30+ in the superficial dermis. Treatment ...
Tópico(s): Nail Diseases and Treatments
2010 - Taylor & Francis | Journal of Cosmetic and Laser Therapy

Maria Isabel de Oliveira, Ana Maria Sardinha Afonso, Cristina Figueiredo Adelaide, Xênia Rawena Medeiros Romeu Lemos, Jalusy Almeida, Ana Lúcia Frugis Yu, Suely Pires Curti,
Measles virus (MV) has been under control in Brazil because of the high coverage rates of immunization.Emphasized by the measles control global program at the Pan American Health Organization, with the use of several strategies of vaccination programs to reduce the number of circulating chains of transmission of the virus, these programs have successfully decreased the incidence of measles on a global scale.However, measles outbreaks still occasionally occur in areas with high vaccine coverage as ...
Tópico(s): Syphilis Diagnosis and Treatment
2014 - UNIVERSIDADE DE SÃO PAULO | Revista do Instituto de Medicina Tropical de São Paulo
Andrea Furtado, Eduardo Carrasquinho, Marco Ferreira, Ana Maria Sardinha Afonso, Fernando Ferrito,
We present a rare case of a schwannoma of the seminal vesicle that occurred in a 43-year-old male with symptoms of the lower urinary tract. Ultrasonography and magnetic resonance imaging documented a solid mass in the patient's left seminal vesicle. A transvesical approach with a transtrigonal midline incision was successfully performed. The microscopic aspect was compatible with schwannoma.
Tópico(s): Urologic and reproductive health conditions
2011 - PZWL Medical Publishers | Central European Journal of Urology

C. A. Figueiredo, Maria Isabel de Oliveira, Suely Pires Curti, Ana Maria Sardinha Afonso, Ana Lúcia Frugis Yu, Jansen de Araújo, Danielle Bruna Leal Oliveira, Edison Luíz Durigon,
Abstract Rubella virus (RV) infection during the early stages of pregnancy can lead to serious birth defects, known as the congenital rubella syndrome (CRS). In 2003, the Pan American Health Organization (PAHO) adopted a resolution calling for the elimination of rubella and the congenital rubella syndrome (CRS) in the Americas by the year 2010. Brazil will have implemented the recommended PAHO strategy for elimination and interruption of endemic rubella virus transmission. The characterization of genotypes ...
Tópico(s): Respiratory viral infections research
2012 - Wiley | Journal of Medical Virology

Maria Isabel de Oliveira, Gislene Mitsue Namiyama, Gabriela Bastos Cabral, João Leandro Ferreira, Noemi Nosomi Taniwaki, Ana Maria Sardinha Afonso, Isabella Rillo Lima, Luís Fernando Macedo de Brigido,
A great variety of viruses which cause exanthema share other clinical manifestations, making the etiologic identification a very difficult task, relying exclusively on the clinical examination. Rubella virus (RV) infection during the early stages of pregnancy can lead to serious birth defects, known as congenital rubella syndrome (CRS). In the present report, we described the presence of Zika virus (ZIKV) particles in urine samples and also ZIKV isolation in SIRC cells from the urine of a patient in ...
Tópico(s): Virology and Viral Diseases
2018 - UNIVERSIDADE DE SÃO PAULO | Revista do Instituto de Medicina Tropical de São Paulo

Cristina Adelaide Figueiredo, Maria Isabel de Oliveira, Ana Maria Sardinha Afonso, Joelma Queiróz Andrade, Maria de Lourdes Brizot, Marcelo Zugaib, Suely Pires Curti,
Human parvovirus B19 infection is known to be one of the causes of hydrops fetalis. The maternal infection caused by the virus may be symptomatic or asymptomatic. In this study, 40 pregnant women with gestational age of approximately 25 weeks, prenatal diagnosis of non immune hydrops fetalis and suspected of human parvovirus B19 infection were studied between January 1999 and December 2005. Serology results and detection of DNA in the maternal serum, foetal serum and amniotic fluid confirmed that ...
Tópico(s): Dermatological and COVID-19 studies
2008 - Sociedade Brasileira de Patologia Clínica | Jornal Brasileiro de Patologia e Medicina Laboratorial

Maria Isabel de Oliveira, Cristina Adelaide Figueiredo, Ana Maria Sardinha Afonso, Fabiana Cristina Pereira dos Santos, Xênia Rawena Medeiros Romeu Lemos, Ana Lucia Frungis Yu, Suely Pires Curti,
Tópico(s): Autism Spectrum Disorder Research
2012 - UNIVERSIDADE DE SÃO PAULO | Revista do Instituto de Medicina Tropical de São Paulo

Cristina Adelaide Figueiredo, Adriana Luchs, Denise Hage Russo, R. Carmona, Ana Maria Sardinha Afonso, Maria Isabel de Oliveira, Suely Pires Curti, José Cássio de Moraes, Cristiana M. Toscano, Flávia Helena Ciccone, María do Carmo Sampaio Tavares Timenetsky,
Tópico(s): Virology and Viral Diseases
2013 - Springer Science+Business Media | Archives of Virology
Maria Isabel de Oliveira, Suely Pires Curti, C. A. Figueiredo, Ana Maria Sardinha Afonso, Márcia Theobaldo, Luiza T. M. de Souza, Stephanie Liffick, Paul A. Rota, William J. Bellini, Edison Luíz Durigon,
Tópico(s): Virology and Viral Diseases
1998 - Sociedade Brasileira de Virologia | VIRUS Reviews & Research
Ana Filipa Duarte, Diogo Alpuim Costa, Nuno Caçador, Ana Magriço Boavida, Ana Maria Sardinha Afonso, Miguel Vilares, Martìn H. Devoto,
Epithelial tumors of the lacrimal gland are rare and usually develop in the orbital lobe. We report the exceedingly rare occurrence of a primary adenoid cystic carcinoma in the palpebral lobe of the lacrimal gland. A 26-year-old female was referred for evaluation of a gradually enlarging mass in the lateral upper eyelid, previously diagnosed as a chalazion. Computed tomography revealed a heterogeneous round lesion anterior to the orbital rim. Excisional biopsy was compatible with an adenoid cystic ...
Tópico(s): Cancer and Skin Lesions
2021 - Taylor & Francis | Orbit

Maria Isabel de Oliveira, Ana Maria Sardinha Afonso, Cristina Adelaide Figueiredo, Suely Pires Curti, Giselle Burlamaqui Klautau, Maria Anice Mureb Sallum,
INTRODUCTION: Human parvovirus B19 (B19V) is associated with a wide range of clinical symptoms.An acute infection can lead to anemia, erythema infectiosum, or hydrops fetalis, as well as arthritis and other clinical manifestations.Among other diseases associated with B19V, several demyelinating disorders may occur as a result of B19V infection.Phylogenetic analysis of partial erythrovirus sequences resulted in three possible B19V genotype groups.Our study involved analysis of samples from a patient ...
Tópico(s): Dermatological and COVID-19 studies
2011 - | Revista Pan-Amazônica de Saúde

Cristina Adelaide Figueiredo, Ana Lúcia Frugis Yu, Ana Maria Sardinha Afonso, Suely Pires Curti, Maria Isabel de Oliveira,
To identify measles virus genotypes in three cases of travelers suspected of measles infection. Samples (blood and urine) were collected for serology, virus isolation, and genotyping. Sera were analyzed for IgM antibodies against measles virus and rubella virus by enzyme-linked immunosorbent assay (ELISA) (Siemens – Marburg, Germany). Clinical samples (lymphocytes and urine) were inoculated into Statens Serum Institute rabbit corneal epithelial cell line- ATCC CL 60 (SIRC) and Vero Slam cells. RNA was ...
Tópico(s): Viral Infections and Immunology Research
2012 - Brazilian Medical Association | Revista da Associação Médica Brasileira

Carla Grasso Figueiredo, Adriana Luchs, Edison Luíz Durigon, Danielle Bruna Leal Oliveira, Vanessa Barbosa da Silveira, Ralyria Mello, Ana Maria Sardinha Afonso, Maria Isabel de Oliveira,
Human cytomegalovirus (HCMV) infections remain a neglected public health issue. The aim of the present study was to evaluate the frequency of HCMV congenital infections in newborns up to 1 month in the Sao Paulo State, from 2010 to 2018. The molecular characterization of HCMV-positive samples was also undertaken. Urine samples from 275 potential congenital HCMV-infected patients were tested by real-time Polymerase Chain Reaction (qPCR). HCMV-positive samples were amplified by conventional PCR targeting ...
Tópico(s): Neonatal Health and Biochemistry
2020 - UNIVERSIDADE DE SÃO PAULO | Revista do Instituto de Medicina Tropical de São Paulo
Manuel Sardinha, José N. Canongia Lopes, Afonso Gusmão, L. Reis, Marco Leite,
Fused Filament Fabrication (FFF) applications are growing at an accelerated rate. As the impact of FFF in industries becomes more significant, accuracy and reliability of parts can compromise its adoption. Warping is a distortion where the surfaces of the printed part do not follow the intended shape of the design and is one of the most common problems when 3D printing with ABS material. In a previous work, ironing, a type of layer-by-layer heat treatment developed to reduce surface roughness, has ...
Tópico(s): Additive Manufacturing Materials and Processes
2022 - Elsevier BV | Procedia Structural Integrity

Cristina Adelaide Figueiredo, Maria Isabel de Oliveira, Ana Maria Sardinha Afonso, Suely Pires Curti,
O presente estudo avaliou a eficiência de vários meios de cultura no isolamento e crescimento do vírus da rubéola inoculado na linhagem celular SIRC. O vírus da rubéola padrão RA27/3 e o vírus selvagem RVi/SãoPaulo/BRA99 (GenBank número DQ458965) foram inoculados na linhagem celular SIRC e cultivada nos meios de cultura 199, DMEM, MEM e RPMI. As células inoculadas observadas em microscopia de fase apresentaram aspecto arredondado, com produção de células multinucleadas. O efeito citopático foi observado ...
Tópico(s): Virus-based gene therapy research
2009 - | REVISTA DO INSTITUTO ADOLFO LUTZ

Cristina Adelaide Figueiredo, Ana Maria Sardinha Afonso, Suely Pires Curti, Maria Isabel de Oliveira, Luisa T.M. Souza, Helena Keico Sato, Raymundo Soares Azevedo,
OBJETIVO: Determinar a prevalência de anticorpos para a rubéola na população de 15 a 39 anos no município de Guaratinguetá, São Paulo, SP. MÉTODOS: Neste estudo, 996 amostras foram colhidas após consentimento informado e esclarecido entre homens e mulheres na faixa etária de 15 a 39 anos. Os anticorpos da classe IgG foram detectados por ELISA usando kit comercial Rubenostika IgGII (Organon Teknika AS, Holland). As faixas etárias foram estratificadas em três categorias: 15-19 anos; 20-29 anos e 30- ...
Tópico(s): HIV/AIDS oral health manifestations
2009 - Brazilian Medical Association | Revista da Associação Médica Brasileira

Maria Isabel de Oliveira, Ana Maria Sardinha Afonso, Cristina Adelaide Figueiredo, Ana Lúcia Frugis Yu, Suely Pires Curti,
No estado de São Paulo, Brasil, em função da eficiente estratégia para a vigilância do vírus do sarampo (VS), não houve registro de casos nativos de sarampo no período de 2001 a 2007. No estado de São Paulo foram registrados casos de sarampo importados, sendo 01 paciente em 2001, outro em 2002 e em 2005 foi alvo de investigação uma criança não vacinada, de 18 meses de idade com exantema e febre, que foi admitida em hospital privado. O Centro de Vigilância Epidemiológica descobriu que o irmão desta ...
Tópico(s): Syphilis Diagnosis and Treatment
2008 - | REVISTA DO INSTITUTO ADOLFO LUTZ
Rodrigo Carvalho, Paula Maio, Daniela Cunha, Isabel Cristina Martins de Freitas, Ana Maria Sardinha Afonso, JORGE ANTÔNIO ORESTES CARDOSO,
ejd.2011.1288 Auteur(s) : Rodrigo CARVALHO1 rodrigoaraujocarvalho@gmail.com, Paula MAIO1, Daniela CUNHA1, Isabel FREITAS1, Ana AFONSO2, Jorge CARDOSO1 1 Department of Dermatology and Venereology, Curry Cabral Hospital, Lisbon, Portugal 2 Department of Pathology, Curry Cabral Hospital, Lisbon, Portugal Reprints.: R. CARVALHO Pemphigus foliaceous (PF) is generally a benign autoimmune blistering disease, but in some patients lesions are severe with serious implications in life quality. We report a [...]
Tópico(s): Urticaria and Related Conditions
2011 - John Libbey Eurotext | European Journal of Dermatology