Jarel K. Gandhi, Travis Knudsen, Matthew Hill, Bhaskar Roy, Lori A. Bachman, Cynthia Pfannkoch‐Andrews, Karina N. Schmidt, Muriel M. Metko, Michael J. Ackerman, Zachary T. Resch, José S. Pulido, Alan D. Marmorstein,
Abstract Human fibrin hydrogels are a popular choice for use as a biomaterial within tissue engineered constructs because they are biocompatible, nonxenogenic, autologous use compatible, and biodegradable. We have recently demonstrated the ability to culture induced pluripotent stem cell (iPSC)-derived retinal pigment epithelium on fibrin hydrogels. However, iPSCs themselves have relatively few substrate options (e.g., laminin) for expansion in adherent cell culture for use in cell therapy. To address ...
Tópico(s): Cellular Mechanics and Interactions
2019 - Wiley | Stem Cells Translational Medicine
Luis Sánchez-Pérez, Timothy Kottke, Gregory A. Daniels, Rosa María Díaz, Jill Thompson, José S. Pulido, Alan Melcher, Richard G. Vile,
Abstract Previously, we showed that nine intradermal injections of a plasmid in which the HSVtk suicide gene is expressed from a melanocyte-specific promoter (Tyr-HSVtk), combined with a plasmid expressing heat shock protein 70 (CMV-hsp70), along with systemic ganciclovir, kills normal melanocytes and raises a CD8+ T cell response that is potent enough to eradicate small, 3-day established B16 tumors. We show in this study that, in that regimen, hsp70 acts as a potent immune adjuvant through TLR-4 ...
Tópico(s): T-cell and B-cell Immunology
2006 - American Association of Immunologists | The Journal of Immunology
Martín Bedolla‐Barajas, Tonatiuh Ramses Bedolla-Pulido, Alan Salvador Camacho-Peña, Estefanía González-García, Jaime Morales‐Romero,
There is limited epidemiological evidence of food hypersensitivity (FH) in the adult population. We aimed to determine the prevalence of FH in Mexican adults, their clinical features and to establish common food involved in its appearance.We designed a cross-sectional study using a fixed quota sampling; 1,126 subjects answered a structured survey to gather information related to FH.The prevalence of FH in adults was 16.7% (95% CI, 14.5% to 18.8%), without statistical significant differences related ...
Tópico(s): Allergic Rhinitis and Sensitization
2014 - | Allergy Asthma and Immunology Research
Adiv A. Johnson, Lori A. Bachman, Benjamin Gilles, Samuel D. Cross, Kimberly E. Stelzig, Zachary T. Resch, Lihua Y. Marmorstein, José S. Pulido, Alan D. Marmorstein,
Purpose: Mutations in BEST1, encoding bestrophin-1 (Best1), cause autosomal recessive bestrophinopathy (ARB). Encoding bestrophin-1 is a pentameric anion channel localized to the basolateral plasma membrane of the RPE. Here, we characterize the effects of the mutations R141H (CGC > CAC) and I366fsX18 (c.1098_1100+7del), identified in a patient in our practice, on Best1 trafficking, oligomerization, and channel activity. Methods: Currents of Cl− were assessed in transfected HEK293 cells using whole-cell ...
Tópico(s): Biotin and Related Studies
2015 - Cadmus Press | Investigative Ophthalmology & Visual Science
Lauren A. Dalvin, José S. Pulido, Alan D. Marmorstein,
Background: Vitelliform dystrophies are a group of macular degenerative diseases characterized by round yellow lesions in the macula. While often idiopathic, vitelliform dystrophies include inherited maculopathies such as Best disease and some cases of pattern dystrophy. The prevalence of vitelliform dystrophies in the United States has not been reported. This study examined the prevalence of vitelliform dystrophies in Olmsted County, Minnesota.Materials and methods: The Rochester Epidemiology Project ...
Tópico(s): Retinal and Optic Conditions
2016 - Taylor & Francis | Ophthalmic Genetics
Jarel K. Gandhi, Zahid Manzar, Lori A. Bachman, Cynthia Andrews‐Pfannkoch, Travis Knudsen, Matthew Hill, Hannah Schmidt, Raymond Iezzi, José S. Pulido, Alan D. Marmorstein,
Recent phase 1 trials of embryonic stem cell and induced pluripotent stem cell (iPSCs) derived RPE transplants for the treatment of macular degeneration have demonstrated the relative safety of this process. However, there is concern over clumping, thickening, folding, and wrinkling of the transplanted RPE. To deliver a flat RPE monolayer, current phase 1 trials are testing synthetic substrates for RPE transplantation. These substrates, however, cause localized inflammation and fibrosis in animal ...
Tópico(s): Retinal and Macular Surgery
2017 - Elsevier BV | Acta Biomaterialia
Jarel K. Gandhi, Fukutaro Mano, Raymond Iezzi, Stephen LoBue, Brad H. Holman, Michael P. Fautsch, Timothy W. Olsen, José S. Pulido, Alan D. Marmorstein,
Retinal pigment epithelium (RPE) transplantation for the treatment of macular degeneration has been studied for over 30 years. Human clinical trials have demonstrated that RPE monolayers exhibit improved cellular engraftment and survival compared to single cell suspensions. The use of a scaffold facilitates implantation of a flat, wrinkle-free, precisely placed monolayer. Scaffolds currently being investigated in human clinical trials are non-degradable which results in the introduction of a chronic ...
Tópico(s): Neuroscience and Neural Engineering
2020 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
Jordana Costa Alves de Assis, Danimar López-Hernández, Eduardo A. Pulido-Murillo, Alan Lane de Melo, Hudson Alves Pinto,
Hippocrepis hippocrepis is a notocotylid that has been widely reported in capybaras; however, the molluscs that act as intermediate hosts of this parasite remain unknown. Furthermore, there are currently no molecular data available for H. hippocrepis regarding its phylogenetic relationship with other members of the family Notocotylidae. In the present study, we collected monostome cercariae and adult parasites from the planorbid Biomphalaria straminea and in the large intestine of capybaras, respectively, ...
Tópico(s): Parasites and Host Interactions
2019 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
Hudson Alves Pinto, Eduardo A. Pulido-Murillo, Alan Lane de Melo, Sara V. Brant,
New larval avian schistosomes found in planorbid snails from Brazil and USA were used for morphological and molecular studies. Eggs with a distinctive long polar filament were found in ducks infected experimentally with Brazilian cercariae. Similar eggs were reported previously in wild or experimentally infected anatids from Brazil, South Africa, and the Czech Republic. Molecular phylogenetic analyses showed that the North American and European schistosomes are sister taxa, which are both sister ...
Tópico(s): Helminth infection and control
2017 - Elsevier BV | Acta Tropica
Produção Nacional
Revisado por pares
Hudson Alves Pinto, Nicole Q. Gonçalves, Danimar López-Hernández, Eduardo A. Pulido-Murillo, Alan Lane de Melo,
Centrocestus formosanus is a foodborne intestinal trematode that is native to Asia and has been introduced into the Americas and Europe. Although there are several studies of C. formosanus in definitive vertebrate hosts (birds and mammals, including humans), and in intermediate vertebrate hosts (fish and amphibians), there is little published information regarding interaction with its transmitting mollusc. In this study we studied the miracidial development of C. formosanus using a mouse as a source ...
Tópico(s): Coccidia and coccidiosis research
2018 - Public Library of Science | PLoS ONE
Produção Nacional
Revisado por pares
André S. Lopes, Eduardo A. Pulido-Murillo, Alan Lane de Melo, Hudson Alves Pinto,
The fishborne zoonotic trematode Haplorchis pumilio (Looss, 1896) is here reported for the first time in Brazil based on morphological, experimental and molecular studies. Pleurolophocercous cercariae emerged from the invasive snail Melanoides tuberculata collected in the municipality of Ceará-Mirim, state of Rio Grande do Norte, Northeast region of Brazil, in November 2018, were used for experimental infection of guppies, Poecilia reticulata. Metacercariae were extracted from the caudal musculature ...
Tópico(s): Helminth infection and control
2020 - Elsevier BV | Infection Genetics and Evolution
Produção Nacional
Revisado por pares
Philippe V. Alves, Jordana Costa Alves de Assis, Danimar López-Hernández, Eduardo A. Pulido-Murillo, Alan Lane de Melo, Sean A. Locke, Hudson Alves Pinto,
Tópico(s): Vector-borne infectious diseases
2020 - Springer Science+Business Media | Parasitology Research
Adiv A. Johnson, Cynthia Andrews‐Pfannkoch, Timothy J. Nelson, José S. Pulido, Alan D. Marmorstein,
The comparison of differentiated induced pluripotent stem cells (iPSCs) derived from patients with disease to differentiated iPSCs derived from healthy patients enables powerful disease modeling. By performing an informal retrospective survey of disease modeling studies published in high impact journals, we found that the median and average number of controls used in these studies were 1 and 1.6, respectively. The bulk of these studies did not control for age, gender and ethnicity. Since a large ...
Tópico(s): CRISPR and Genetic Engineering
2017 - Future Medicine | Regenerative Medicine
Ilhan Cem Duru, Anne Ylinen, Sergei Belanov, Alan Ávila Pulido, Lars Paulín, Petri Auvinen,
Abstract Background Psychrotrophic lactic acid bacteria (LAB) species are the dominant species in the microbiota of cold-stored modified-atmosphere-packaged food products and are the main cause of food spoilage. Despite the importance of psychrotrophic LAB, their response to cold or heat has not been studied. Here, we studied the transcriptome-level cold- and heat-shock response of spoilage lactic acid bacteria with time-series RNA-seq for Le. gelidum, Lc. piscium , and P. oligofermentans at 0 °C, 4 ° ...
Tópico(s): Meat and Animal Product Quality
2021 - BioMed Central | BMC Genomics
Adiv A. Johnson, Karina E Guziewicz, C. Justin Lee, Ravi C. Kalathur, José S. Pulido, Lihua Y. Marmorstein, Alan D. Marmorstein,
Mutations in the gene BEST1 are causally associated with as many as five clinically distinct retinal degenerative diseases, which are collectively referred to as the “bestrophinopathies”. These five associated diseases are: Best vitelliform macular dystrophy, autosomal recessive bestrophinopathy, adult-onset vitelliform macular dystrophy, autosomal dominant vitreoretinochoroidopathy, and retinitis pigmentosa. The most common of these is Best vitelliform macular dystrophy. Bestrophin 1 (Best1), the ...
Tópico(s): Photoreceptor and optogenetics research
2017 - Elsevier BV | Progress in Retinal and Eye Research
José S. Pulido, Jeffrey S. Heier, Alan D. Marmorstein,
Tópico(s): Sarcoma Diagnosis and Treatment
2017 - Massachusetts Medical Society | New England Journal of Medicine
Produção Nacional
Revisado por pares
Eduardo A. Pulido-Murillo, Luis Fernando Viana Furtado, Alan Lane de Melo, Élida Mara Leite Rabelo, Hudson Alves Pinto,
We investigated the transmission of the fishborne trematodes Centrocestus formosanus and Haplorchis pumilio by Melanoides tuberculata snails in Peru. We report on results of experimental, morphological, and molecular approaches and discuss the potential risk for future human cases, given the existence of food habits in the country involving the ingestion of raw fish.
Tópico(s): Primate Behavior and Ecology
2018 - Centers for Disease Control and Prevention | Emerging infectious diseases
Lauren A. Dalvin, Adiv A. Johnson, José S. Pulido, Ranjit S. Dhaliwal, Alan D. Marmorstein,
A previous report demonstrated antibodies to bestrophin in paraneoplastic exudative polymorphous vitelliform maculopathy (PEPVM). Other cases demonstrated antibodies to different proteins in the retinal pigment epithelium (RPE). In this report, serum was analyzed to determine whether a patient with PEPVM and a reduced Arden ratio had developed autoantibodies to human Bestrophin-1 (Best1).Human embryonic kidney 293 cells (HEK293) were transfected with Best1 and stained with an antibody specific to Best1 ( ...
Tópico(s): Retinal Diseases and Treatments
2015 - Association for Research in Vision and Ophthalmology | Translational Vision Science & Technology
Produção Nacional
Revisado por pares
André S. Lopes, Eduardo A. Pulido-Murillo, Danimar López-Hernández, Alan Lane de Melo, Hudson Alves Pinto,
Xiphidiocercariae were found in the invasive snail Melanoides tuberculata collected during a malacological survey in Ceará-Mirim, State of Rio Grande do Norte, Northeastern Brazil in November 2018 and submitted to morphological and molecular analyses. The morphology revealed similarities between the larvae here reported for the first time in M. tuberculata from Brazil and other xiphidiocercariae described in thiarid snails from Asia and Africa. Phylogenetic analyses based on 28S and ITS-2 sequences ...
Tópico(s): Parasites and Host Interactions
2021 - Elsevier BV | Parasitology International
Produção Nacional
Jordana Costa Alves de Assis, Eduardo A. Pulido-Murillo, Alan Lane de Melo, Nelson Rodrigo da Silva Martins, Hudson Alves Pinto,
Philophthalmosis is a zoonotic ocular disease that affects mainly domestic and wild avian species in different parts of the world. However, aspects related to its epizoology in domestic animals are not completely known. In the present study, 47 Muscovy duck (Cairina moschata), 8 mallards (Anas platyrhynchos platyrhynchos) and 6 African geese (Anser cygnoides), free-living in an urban lake in Belo Horizonte, Minas Gerais, Brazil, were evaluated for eye fluke infection. The animals were manually contained, ...
Tópico(s): Helminth infection and control
2022 - Elsevier BV | Veterinary Parasitology Regional Studies and Reports
Phonphimon Wongthida, Rosa María Díaz, Feorillo Galivo, Timothy Kottke, Jill Thompson, José S. Pulido, Kevin D. Pavelko, Larry R. Pease, Alan Melcher, Richard G. Vile,
Abstract Innate immune effector mechanisms triggered by oncolytic viruses may contribute to the clearance of both infected and uninfected tumor cells in immunocompetent murine hosts. Here, we developed an in vitro tumor cell/bone marrow coculture assay and used it to dissect innate immune sensor and effector responses to intratumoral vesicular stomatitis virus (VSV). We found that the type III IFN interleukin-28 (IL-28) was induced by viral activation of innate immune-sensing cells, acting as a key ...
Tópico(s): CAR-T cell therapy research
2010 - American Association for Cancer Research | Cancer Research
Timothy Kottke, Luis Sánchez-Pérez, Rosa María Díaz, Jill Thompson, Heung Chong, Kevin J. Harrington, Stuart K. Calderwood, José S. Pulido, Nikolaos T. Georgopoulos, Peter J. Selby, Alan Melcher, Richard G. Vile,
Abstract A close connectivity between autoimmune and tumor rejection responses is known to exist in the case of melanoma immunotherapy. However, relatively little is known about self-antigens on other types of normal cells, their relation to the development of autoimmune disease, and their possible coexistence as potential tumor rejection antigens on associated tumors. In the current study, we induced inflammatory killing of normal prostate tissue in situ using a fusogenic membrane glycoprotein ...
Tópico(s): Cancer Immunotherapy and Biomarkers
2007 - American Association for Cancer Research | Cancer Research
Amanda L. Huff, Phonphimon Wongthida, Timothy Kottke, Jill Thompson, Christopher B. Driscoll, Matthew Schuelke, Kevin G. Shim, Reuben S. Harris, Amy M. Molan, José S. Pulido, Peter J. Selby, Kevin J. Harrington, Alan Melcher, Laura Evgin, Richard G. Vile,
Tumor cells frequently evade applied therapies through the accumulation of genomic mutations and rapid evolution. In the case of oncolytic virotherapy, understanding the mechanisms by which cancer cells develop resistance to infection and lysis is critical to the development of more effective viral-based platforms. Here, we identify APOBEC3 as an important factor that restricts the potency of oncolytic vesicular stomatitis virus (VSV). We show that VSV infection of B16 murine melanoma cells upregulated ...
Tópico(s): Animal Virus Infections Studies
2018 - Elsevier BV | Molecular Therapy — Oncolytics
Fukutaro Mano, Jarel K. Gandhi, Raphael Pereira da Silva, Aline Do Amaral Silva, Lucas Iezzi, Raymond Iezzi, José S. Pulido, Alan D. Marmorstein,
To improve outcomes for subretinal implantation surgery in pigs.Analysis of variables affecting the success of subretinal implantation surgery was performed on videos of 37 surgeries. Ex vivo experiments were conducted to measure intraocular pressure (IOP) and test various prototyped implanters for effectiveness at maintaining IOP.A video analysis revealed a prolonged sclerotomy open time owing to a combination of uncontrolled bleeding and excessive fluid outflow often resulting in retinal prolapse. ...
Tópico(s): Retinal Diseases and Treatments
2022 - Association for Research in Vision and Ophthalmology | Translational Vision Science & Technology
Sunny Abraham, Michael J. Hadd, Lan P. Tran, Troy Vickers, Janice A. Sindac, Zdravko V. Milanov, Mark W. Holladay, Shripad S. Bhagwat, Helen Hua, Julia M. Ford Pulido, Merryl Cramer, Dana Gitnick, Joyce James, Alan Dao, Barbara Belli, Robert C. Armstrong, Daniel K. Treiber, Gang Liu,
The synthesis and SAR for a novel series of pyrrolotriazines as pan-Aurora kinase inhibitors are described. Optimization of the cyclopropane carboxamide terminus of lead compound 1 resulted in analogs with high cellular activity and improved rat PK profiles. Notably, compound 17l demonstrated tumor growth inhibition in a mouse xenograft model.
Tópico(s): Cancer therapeutics and mechanisms
2011 - Elsevier BV | Bioorganic & Medicinal Chemistry Letters
Christopher B. Driscoll, Matthew Schuelke, Timothy Kottke, Jill Thompson, Phonphimon Wongthida, Jason M. Tonne, Amanda L. Huff, Amber Miller, Kevin G. Shim, Amy M. Molan, Cynthia Wetmore, Peter J. Selby, Adel Samson, Kevin J. Harrington, Hardev Pandha, Alan Melcher, José S. Pulido, Reuben S. Harris, Laura Evgin, Richard G. Vile,
Abstract APOBEC3B, an anti-viral cytidine deaminase which induces DNA mutations, has been implicated as a mediator of cancer evolution and therapeutic resistance. Mutational plasticity also drives generation of neoepitopes, which prime anti-tumor T cells. Here, we show that overexpression of APOBEC3B in tumors increases resistance to chemotherapy, but simultaneously heightens sensitivity to immune checkpoint blockade in a murine model of melanoma. However, in the vaccine setting, APOBEC3B-mediated ...
Tópico(s): CAR-T cell therapy research
2020 - Nature Portfolio | Nature Communications
Álvaro Gómez‐Gutiérrez, Susanne Schnabel, Joaquín Francisco Lavado Contador, José Juan de Sanjosé Blasco, Alan Atkinson, Manuel Pulido Fernández, Manuel Sánchez-Fernández,
Gully erosion in agrosilvopastoral systems of SW Spain represents a common degradation process, but has been hardly analysed. The suitability of using the Unmanned Aerial Vehicles (UAV) and Structure from Motion photogrammetry (SfM) workflow to map small valley-bottom gullies in these landscapes was tested. The results showed centimetre-level accuracy. Observed strengths and limitations of the UAV+SfM workflow in the study areas are discussed. The resulting cartography allowed mapping soil erosion ...
Tópico(s): Aeolian processes and effects
2018 - Asociación Española de Geografía | Boletín de la Asociación de Geógrafos Españoles
Christopher Miller, Abdo Kabarriti, José Zago Pulido, Justin Ziemba, Thomas J. Guzzo, Alan Wein, S. Bruce Malkowicz,
... TERTIARY CARE CENTER Christopher Miller, Abdo Kabarriti, Jose Pulido, Justin Ziemba, Thomas Guzzo, Alan Wein, and S. Bruce Malkowicz Christopher MillerChristopher Miller ... Abdo Kabarriti More articles by this author Jose Pulido More articles by this author Justin Ziemba More articles by this author Thomas Guzzo More articles by this author Alan Wein More articles by this author S. Bruce ...
Tópico(s): Health Promotion and Cardiovascular Prevention
2016 - Lippincott Williams & Wilkins | The Journal of Urology
Timothy Kottke, Jill Thompson, Rosa María Díaz, José S. Pulido, Candice Willmon, Matt Coffey, Peter J. Selby, Alan Melcher, Kevin J. Harrington, Richard G. Vile,
Abstract Purpose: The goals of this study were (a) to investigate whether preconditioning of immunocompetent mice with PC-61-mediated regulatory T-cell (Treg) depletion and interleukin-2 (IL-2) would enhance systemic delivery of reovirus into subcutaneous tumors and (b) to test whether cyclophosphamide (CPA), which is clinically approved, could mimic PC-61 for modification of Treg activity for translation into the next generation of clinical trials for intravenous delivery of reovirus. Experimental Design: ...
Tópico(s): Immune Cell Function and Interaction
2009 - American Association for Cancer Research | Clinical Cancer Research
Timothy Kottke, John Chester, Elizabeth J. Ilett, Jill Thompson, Rosa María Díaz, Matt Coffey, Peter J. Selby, Gerard J. Nuovo, José S. Pulido, Debabrata Mukhopadhyay, Hardev Pandha, Kevin J. Harrington, Alan Melcher, Richard G. Vile,
We have previously reported that a burst of vascular endothelial growth factor (VEGF) signaling to tumor-associated endothelium induces a proviral state, during which systemically delivered oncolytic reovirus can replicate in endothelium, thereby inducing immune-mediated vascular collapse and significant antitumor therapy. Using chimeric receptors, we show here that induction of the proviral state proceeds through VEGFR2, but not VEGFR1, signaling in endothelial cells. In contrast, innate immune ...
Tópico(s): Viral Infectious Diseases and Gene Expression in Insects
2011 - Elsevier BV | Molecular Therapy