Rabih M. Salloum, Wiley W. Souba, Alarico Fernandez, Bruce R. Stevens,
The effects of a glutamine-enriched diet on the transport of glutamine across brush border membrane vesicles (BBMV) from the rat jejunum were studied to gain further insight into the effects of diet on regulating gut glutamine utilization. Following fasting, rats were randomized to one of three nutritionally complete elemental diets supplemented with glutamine, glutamate, or glycine (control). Brush border membrane vesicles were prepared by a Mg2+ aggregation/differential centrifugation technique ...
Tópico(s): Metabolism and Genetic Disorders
1990 - Elsevier BV | Journal of Surgical Research
Bruce R. Stevens, Alarico Fernandez, Christine Kneer, J J Cerda, M. Ian Phillips, Edward R. Woodward,
Angiotensin-converting enzyme (ACE) has been identified as a prominent brush border membranebound enzyme of human jejunum.In this study, we purified brush border membrane vesicles enriched in ACE, and characterized the ACE with regard to (a) its stability in the membrane, (b) substrate hydrolysis kinetics compared with pulmonary endothelial ACE, and (c) pharmacologic interaction with Ramipril.These investigations resulted in the following findings.The uninhibited enzyme is stable in native membranes ...
Tópico(s): Enzyme function and inhibition
1988 - Elsevier BV | Gastroenterology
Susana Alarico, Fred A. Rainey, Nuno Empadinhas, Peter Schümann, M. Fernanda Nobre, Milton S. da Costa,
A bacterial isolate, with an optimum growth temperature of about 50 degrees C, was recovered from the hot spring at Egerszalók in Hungary. Phylogenetic analyses using the 16S rRNA gene sequence of strain H-8T indicated that the new organism represented a new genus and species of alpha-1 subclass of the Proteobacteria. The major fatty acids of strain H-8T are 16:0, 18:1 omega7c; the rare fatty acid 19:0 20H cyclo 11,12 is also present. Ubiquinone 9 is the major respiratory quinone, the polar lipids ...
Tópico(s): Protist diversity and phylogeny
2002 - Elsevier BV | Systematic and Applied Microbiology
Zélia Silva, Susana Alarico, Ana Nobre, Reinhold Horlacher, Joey D. Marugg, Winfried Boos, Ana Mingote, Milton S. da Costa,
Strains of Thermus thermophilus accumulate primarily trehalose and smaller amounts of mannosylglycerate in response to salt stress in yeast extract-containing media (O. C. Nunes, C. M. Manaia, M. S. da Costa, and H. Santos, Appl. Environ. Microbiol. 61:2351-2357, 1995). A 2.4-kbp DNA fragment from T. thermophilus strain RQ-1 carrying otsA (encoding trehalose-phosphate synthase [TPS]), otsB (encoding trehalose-phosphate phosphatase [TPP]), and a short sequence of the 5' end of treS (trehalose synthase [ ...
Tópico(s): Legume Nitrogen Fixing Symbiosis
2003 - American Society for Microbiology | Journal of Bacteriology
Susana Alarico, Milton S. da Costa, Nuno Empadinhas,
ABSTRACT Trehalose supports the growth of Thermus thermophilus strain HB27, but the absence of obvious genes for the hydrolysis of this disaccharide in the genome led us to search for enzymes for such a purpose. We expressed a putative α-glucosidase gene (TTC0107), characterized the recombinant enzyme, and found that the preferred substrate was α,α-1,1-trehalose, a new feature among α-glucosidases. The enzyme could also hydrolyze the disaccharides kojibiose and sucrose (α-1,2 linkage), nigerose and ...
Tópico(s): Microbial Metabolites in Food Biotechnology
2008 - American Society for Microbiology | Journal of Bacteriology
This paper presents the initial results of an experimental investigation of regulated and unregulated emissions of a boiler fueled with biodiesel and heating oil. In particular CO, SO2, NOx, particulate matter (PM), Polycyclic Aromatic Hydrocarbons (PAH), Volatile Organic Compounds (VOC) and aldehydes emissions were examined. When using biodiesel, a strong reduction in CO and PM emissions was recorded with respect to home heating oil. The PAHs contained in the PM in the case of biodiesel were nearly ...
Tópico(s): Advanced Combustion Engine Technologies
2008 - Elsevier BV | Energy
Ana Nobre, Susana Alarico, Chantal Fernandes, Nuno Empadinhas, Milton S. da Costa,
Trehalose is the primary organic solute in Rubrobacter xylanophilus under all conditions tested, including those for optimal growth. We detected genes of four different pathways for trehalose synthesis in the genome of this organism, namely, the trehalose-6-phosphate synthase (Tps)/trehalose-6-phosphate phosphatase (Tpp), TreS, TreY/TreZ, and TreT pathways. Moreover, R. xylanophilus is the only known member of the phylum Actinobacteria to harbor TreT. The Tps sequence is typically bacterial, but the ...
Tópico(s): Fungal and yeast genetics research
2008 - American Society for Microbiology | Journal of Bacteriology
Abraham Gemio Alarico, Nahuel Romero, Laura Hernández Martínez, Silvia Catalá, David E. Gorla,
The Bolivian Chaco is part of the endemic region of Chagas disease and an area where pyrethroid resistant Triatoma infestans (Hemiptera: Reduviidae) populations has been reported. The World Health Organization identified these resistant populations as an important focus for research. The objective of this study was to evaluate the residual effect of a micro-encapsulated formulation containing organophosphate active ingredients and a juvenile hormone analogue (Inesfly 5A IGR) on the mortality of T. ...
Tópico(s): Insect symbiosis and bacterial influences
2010 - Instituto Oswaldo Cruz, Ministério da Saúde | Memórias do Instituto Oswaldo Cruz
Susana Alarico, Nuno Empadinhas, Ana Mingote, Catarina Simões, Maria S. Santos, Milton S. da Costa,
We disrupted the mpgS encoding mannosyl-3-phosphoglycerate synthase (MpgS) of Thermus thermophilus strains HB27 and RQ-1, by homologous recombination, to assess the role of the compatible solute mannosylglycerate (MG) in osmoadaptation of the mutants, to examine their ability to grow in NaCl-containing medium and to identify the intracellular organic solutes. Strain HB27 accumulated only MG when grown in defined medium containing 2% NaCl; mutant HB27M9 did not grow in the same medium containing more ...
Tópico(s): Photosynthetic Processes and Mechanisms
2007 - Springer Science+Business Media | Extremophiles
Alarico Macor, Antonio Rossetti,
Tópico(s): Gear and Bearing Dynamics Analysis
2011 - Elsevier BV | Mechanism and Machine Theory
Alarico Macor, F. Avella, Davide Faedo,
Two Euro 3 commercial trucks fuelled with a 30% v/v biodiesel/diesel fuel blend (B30) and pure diesel fuel were tested in laboratory under the standard driving conditions (UDC and EUDC driving cycles) and the CADC "URBAN" test cycle, in order to evaluate the fuel consumption, regulated (CO, HC, NOx, PM) and unregulated emissions (aldehydes and polycyclic aromatic hydrocarbons). After substitution of diesel fuel with B30 the following results were observed. The fuel economy increased proportionally to ...
Tópico(s): Advanced Combustion Engine Technologies
2011 - Elsevier BV | Applied Energy
V. Mendes, Ana Maranha, Susana Alarico, Milton S. da Costa, Nuno Empadinhas,
Mycobacteria synthesize intracellular methylglucose lipopolysaccharides (MGLP) proposed to regulate fatty acid synthesis. Although their structures have been elucidated, the identity of most biosynthetic genes remains unknown. The first step in MGLP biosynthesis is catalyzed by a glucosyl-3-phosphoglycerate synthase (GpgS, Rv1208 in Mycobacterium tuberculosis H37Rv). However, a typical glucosyl-3-phosphoglycerate phosphatase (GpgP, EC3.1.3.70) for dephosphorylation of glucosyl-3-phosphoglycerate to ...
Tópico(s): Carbohydrate Chemistry and Synthesis
2011 - Nature Portfolio | Scientific Reports
Igor Tiago, Ana Maranha, V. Mendes, Susana Alarico, Patrick J. Moynihan, Anthony J. Clarke, Sandra Macedo‐Ribeiro, Pedro José Barbosa Pereira, Nuno Empadinhas,
ABSTRACT Mycobacterium hassiacum is a rapidly growing mycobacterium isolated from human urine and so far the most thermophilic among mycobacterial species. Its thermotolerance and phylogenetic relationship to M. tuberculosis render its proteins attractive tools for crystallization and structure-guided drug design. We report the draft genome sequence of M. hassiacum DSM 44199.
Tópico(s): Tuberculosis Research and Epidemiology
2012 - American Society for Microbiology | Journal of Bacteriology
Marika Tardella, Marwin Gutiérrez, Fausto Salaffi, Marina Carotti, Alarico Ariani, Chiara Bertolazzi, Emilio Filippucci, Walter Grassi,
To investigate the correlation between ultrasound (US) B-lines and high-resolution computed tomography (HRCT) findings in the assessment of pulmonary fibrosis (PF) in patients with connective tissue disorders (CTD).Thirty-four patients with a diagnosis of CTD were included. Each patient underwent clinical examination, pulmonary function test (PFT), chest HRCT, and lung US by an experienced radiologist or rheumatologist. A second rheumatologist carried out US examinations to assess interobserver agreement. ...
Tópico(s): Pulmonary Hypertension Research and Treatments
2012 - The Journal of Rheumatology Publishing Company Limited | The Journal of Rheumatology
V. Mendes, Ana Maranha, Susana Alarico, Nuno Empadinhas,
Covering: up to the end of 2011
Tópico(s): Immune Response and Inflammation
2012 - Royal Society of Chemistry | Natural Product Reports
Antonio Rossetti, Alarico Macor,
In the design of a hydro-mechanical transmission the achievement of high efficiency is one of the most important objectives. Furthermore, the available space for the transmission is always limited, constraining the design process. In this paper, therefore, the design of a hydro-mechanical transmission has been set as a multi-objective optimization problem, where the goal is not only the best efficiency but also the smallest size of the transmission. A calculation procedure for a “Pareto optimization” ...
Tópico(s): Gear and Bearing Dynamics Analysis
2012 - Elsevier BV | Mechanism and Machine Theory
Susana Alarico, Nuno Empadinhas, Catarina Simões, Zélia Silva, Anke Henne, Ana Mingote, Helena Santos, Milton S. da Costa,
ABSTRACT In this study we correlate the presence of genes leading to the synthesis of trehalose and mannosylglycerate (MG) in 17 strains of the genus Thermus with the ability of the strains to grow and accumulate these compatible solutes in a defined medium containing NaCl. The two sets of genes, namely, otsA / otsB for the synthesis of trehalose and mpgS / mpgP for the synthesis of MG, were necessary for the growth of Thermus thermophilus in a defined medium containing up to 6% NaCl. Strains lacking ...
Tópico(s): Microbial Metabolites in Food Biotechnology
2005 - American Society for Microbiology | Applied and Environmental Microbiology
Jungdon Bae, Kwang‐Ho Kim, Doo‐Il Kim, Yongseok Choi, Joong Su Kim, Sukhoon Koh, Suk‐In Hong, Dae‐Sil Lee,
... J. org. Chem. 2004, 5047. 16Z. Silva, S. Alarico, M. S. da Costa, Extremophiles 2005, 9, 29. ...
Tópico(s): Enzyme Production and Characterization
2005 - Wiley | ChemBioChem
Produção Nacional
Revisado por pares
F. C. R. Fioravante, Maria de Fátima Costa Alves, Eleuse Machado de Britto Guimarães, Marília Dalva Turchi, Heitor Alarico G. Freitas, Ludmilla Thomé Domingos,
Few data are available on the prevalence and risk factors for Chlamydia trachomatis infection among young men in Brazil.To assess prevalence and risk factors for C. trachomatis infection in male military conscripts.In 2000, 627 young men recruited for military service in Goiania, Goias, Brazil, were enrolled in this cross-sectional study. Participants completed a demographic and sexual risk behavior questionnaire, and urine samples were screened for C. trachomatis by polymerase chain reaction.The ...
Tópico(s): Urinary Tract Infections Management
2005 - Lippincott Williams & Wilkins | Sexually Transmitted Diseases
Corrado Giannantoni, Andrea Lazzaretto, Alarico Macor, Alberto Mirandola, Anna Stoppato, S. Tonon, Sérgio Ulgiati,
An iterative procedure is suggested to evaluate and improve the energy system design. The procedure considers the information deriving from complementary evaluation approaches, each applied within its appropriate time-space window of interest: (a) Process-related, local-scale methods (Energy, Exergy and Thermoeconomic analyses); (b) Environmental assessment methods (Impact Assessment, Emergy Synthesis); (c) Economic methods (Micro- and Macro-Economic and Externality Evaluations). Process-related methods ...
Tópico(s): Global Energy and Sustainability Research
2005 - Elsevier BV | Energy
Zélia Silva, Susana Alarico, Milton S. da Costa,
The genes for trehalose synthesis in Thermus thermophilus RQ-1, namely otsA [trehalose-phosphate synthase (TPS)], otsB [trehalose-phosphate phosphatase (TPP)], and treS [trehalose synthase (maltose converting) (TreS)] genes are structurally linked. The TPS/TPP pathway plays a role in osmoadaptation, since mutants unable to synthesize trehalose via this pathway were less osmotolerant, in trehalose-deprived medium, than the wild-type strain. The otsA and otsB genes have now been individually cloned and overexpressed ...
Tópico(s): Fungal and yeast genetics research
2004 - Springer Science+Business Media | Extremophiles
Nuno Borges, Joey D. Marugg, Nuno Empadinhas, Milton S. da Costa, Helena Santos,
... 152) Google Scholar), and T. thermophilus (33Silva Z. Alarico S. Nobre A. Horlacher R. Marugg J. Boos ...
Tópico(s): Enzyme Structure and Function
2004 - Elsevier BV | Journal of Biological Chemistry
Ana Nobre, Susana Alarico, Ana Maranha, V. Mendes, Nuno Empadinhas,
Trehalose is a natural glucose disaccharide identified in the 19th century in fungi and insect cocoons, and later across the three domains of life. In members of the genus Mycobacterium , which includes the tuberculosis (TB) pathogen and over 160 species of nontuberculous mycobacteria (NTM), many of which are opportunistic pathogens, trehalose has been an important focus of research over the last 60 years. It is a crucial player in the assembly and architecture of the remarkable mycobacterial cell envelope ...
Tópico(s): Infectious Diseases and Mycology
2014 - Microbiology Society | Microbiology
Christoph Legat, Jakob Mund, Alarico Campetelli, Georg Hackenberg, Jens Folmer, Daniel Schütz, Manfred Broy, Birgit Vogel‐Heuser,
Abstract To facilitate engineering and evolution of automation systems, ensuring the correctness of the design models is an important topic. Industrial automation systems are composed of various heterogeneous elements designed by different disciplines such as mechanical, electrical/electronic and software engineering. In this contribution, an approach for modeling industrial automation systems is presented which is based on interface behavior modeling of design artifacts and which supports automatic ...
Tópico(s): Business Process Modeling and Analysis
2014 - R. Oldenbourg Verlag | at - Automatisierungstechnik
Kathleen M. Maloney, Jenny Ancca‐Juárez, Renzo Salazar, Katty Borrini-Mayorí, Malwina Niemierko, Joshua Yukich, César Náquira, Joseph Keating, Michael Z. Levy,
... Dias and Jemmio 2008, Amelotti et al. 2009, Alarico et al. 2009), and malaria (Mosqueira et al. ...
Tópico(s): Insect symbiosis and bacterial influences
2013 - Wiley | Journal of Vector Ecology
Alarico Ariani, Marina Carotti, Marwin Gutiérrez, Elisabetta Bichisecchi, Walter Grassi, Gian Marco Giuseppetti, Fausto Salaffi,
Tópico(s): Voice and Speech Disorders
2013 - Springer Science+Business Media | Rheumatology International
Alarico Macor, Antonio Rossetti,
The reduction of fuel consumption of urban vehicles is one of the challenges of the society today. Many promising technologies such as hybrid electric vehicles and fuel cells are the focus of a lot of research and investments, but high costs prevent their development on a large scale, especially in heavy duty vehicles. Simpler solutions, such as continuously variable transmissions, are then interesting alternatives: they preserve some of the advantages of hybrid solutions significantly reducing ...
Tópico(s): Advanced Combustion Engine Technologies
2013 - Elsevier BV | Energy Conversion and Management
Marwin Gutiérrez, Luca Di Geso, João Rovisco, Marco Di Carlo, Alarico Ariani, Emilio Filippucci, Walter Grassi,
To describe the learning curve of rheumatologists with limited experience using ultrasound (US) attending an intensive disease-oriented training program focusing on the skills required to obtain and interpret US signs of monosodium urate (MSU) crystal deposits in joint and periarticular tissues.Three investigators participated in a 7-day training program involving 12 men with gout. The agreement between the expert and beginners was calculated in 4 sessions involving 8 patients with gout. The US assessment ...
Tópico(s): Case Reports on Hematomas
2013 - Wiley | Arthritis Care & Research
Fausto Salaffi, Marina Carotti, Alessandro Ciapetti, Alarico Ariani, Stefania Gasparini, Walter Grassi,
To investigate the performance of conventional radiography (CR) for the detection of bone erosions of wrist in rheumatoid arthritis (RA) using multidetector computed tomography (CT) as the reference method and to evaluate the validity of a computer-assisted manual segmentation (outlining) technique to quantify erosion volume on CT scans. Twenty five RA patients and six controls underwent CT and radiographic evaluation of the dominant wrist on the same day. CT was performed by using a 64 GE light Speed ...
Tópico(s): Orthopedic Surgery and Rehabilitation
2013 - BioMed Central | BMC Musculoskeletal Disorders
Andrea Lazzaretto, Alarico Macor,
Most of the thermoeconomic accounting and optimization methods for energy systems are based upon a definition of the productive purpose for each component. On the basis of this definition, a productive structure of the system can be defined in which the interactions among the components are described by their fuel product. The aim of this work is to calculate marginal and average unit costs of the exergy flows starting from their definitions by a direct inspection of the productive structure. As ...
Tópico(s): Advanced Thermodynamics and Statistical Mechanics
1995 - ASM International | Journal of Energy Resources Technology