Produção Nacional
Revisado por pares
Elisabeth de Amorim Machado, Fernanda Jannuzzi, Silvio de Cássio Costa Telles, Cecília Lacroix de Oliveira, Isabel Rey, Fernando Lencastre Sicuro, Maria das Graças Coelho de Souza, Alexandra Monteiro, Eliete Bouskela, Paulo Ferrez Collett‐Solberg, Paulo Farinatti,
The benefits of swimming as a treatment for overweight children are undefined. We investigated the effects of recreational swimming on cardiometabolic risk in children/adolescents with normal and excess weight. Participants (n = 49, 26 girls, 10.3 ± 1.8 y) were grouped as ‘eutrophic swimming’ (EU-Swim, n = 14); ‘excess weight swimming’ (EW-Swim, n = 20) with an ‘obese swimming’ subgroup (OB-Swim, n = 10); and ‘excess weight sedentary’ (EW-Sed, n = 15) with an ‘obese sedentary’ subgroup (OB-Sed, n = 11). Swimming (50 min, ...
Tópico(s): Cardiovascular and exercise physiology
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Environmental Research and Public Health
Alexandra S. Simões, Carina Valente, Hermı́nia de Lencastre, Raquel Sá‐Leão,
Tópico(s): Pneumocystis jirovecii pneumonia detection and treatment
2011 - Elsevier BV | Diagnostic Microbiology and Infectious Disease
Carina Valente, Suzanne Dawid, Francisco Pinto, Jason Hinds, Alexandra S. Simões, Katherine A. Gould, Luís André Mendes, Hermı́nia de Lencastre, Raquel Sá‐Leão,
ABSTRACT Nasopharyngeal colonization is important for Streptococcus pneumoniae evolution, providing the opportunity for horizontal gene transfer when multiple strains co-occur. Although colonization with more than one strain of pneumococcus is common, the factors that influence the ability of strains to coexist are not known. A highly variable blp (bacteriocin-like peptide) locus has been identified in all sequenced strains of S. pneumoniae . This locus controls the regulation and secretion of bacteriocins, ...
Tópico(s): Streptococcal Infections and Treatments
2016 - American Society for Microbiology | Applied and Environmental Microbiology
Dora Rolo, Alexandra S. Simões, Arnau Domenech, Asunción Fenoll, Josefina Liñares, Hermı́nia de Lencastre, Carmen Ardanuy, Raquel Sá‐Leão,
We aimed to obtain insights on the nature of a collection of isolates presumptively identified as atypical Streptococcus pneumoniae recovered from invasive and non-invasive infections in Spain. One-hundred and thirty-two isolates were characterized by: optochin susceptibility in ambient and CO(2)-enriched atmosphere; bile solubility; PCR-based assays targeting pneumococcal genes lytA, ply, pspA, cpsA, Spn9802, aliB-like ORF2, and a specific 16S rRNA region; multilocus sequence analysis; and antimicrobial ...
Tópico(s): Streptococcal Infections and Treatments
2013 - Public Library of Science | PLoS ONE
Débora A. Tavares, Alexandra S. Simões, Hester J. Bootsma, Peter W. M. Hermans, Hermı́nia de Lencastre, Raquel Sá‐Leão,
Pneumococcus is a major human pathogen and the polysaccharide capsule is considered its main virulence factor. Nevertheless, strains lacking a capsule, named non-typeable pneumococcus (NT), are maintained in nature and frequently colonise the human nasopharynx. Interest in these strains, not targeted by any of the currently available pneumococcal vaccines, has been rising as they seem to play an important role in the evolution of the species. Currently, there is a paucity of data regarding this group ...
Tópico(s): Respiratory viral infections research
2014 - BioMed Central | BMC Genomics
Alexandra S. Simões, Liliana Pereira, Sónia Nunes, António Brito‐Avô, Hermı́nia de Lencastre, Raquel Sá‐Leão,
ABSTRACT The introduction of the seven-valent pneumococcal conjugate vaccine (PCV7) in Portugal led to extensive serotype replacement among carriers of pneumococci, with a marked decrease of PCV7 types. Although antimicrobial resistance was traditionally associated with PCV7 types, no significant changes in the rates of nonsusceptibility to penicillin, resistance to macrolides, or multidrug resistance were observed. This study aimed to investigate the mechanisms leading to maintenance of antimicrobial ...
Tópico(s): Respiratory viral infections research
2011 - American Society for Microbiology | Journal of Clinical Microbiology
Raquel Sá‐Leão, Alexandra S. Simões, Sónia Nunes, Natacha Gonçalves‐Sousa, Nelson Frazão, Hermı́nia de Lencastre,
The authors aimed to get insights into the population structure of non-(sero)typable pneumococci (NTPn), a specific group of natural atypical pneumococci whose identification is often difficult, and which has remained insufficiently studied. A total of 265 presumptive NTPn, isolated between 1997 and 2003 from the nasopharynx of children, were characterized. Strains were confirmed to be pneumococci on the basis of bile solubility, and PCR detection or Southern blotting hybridization of lytA and psaA, ...
Tópico(s): Respiratory viral infections research
2006 - Microbiology Society | Microbiology
Sofia Félix, Sara Handem, Sónia Nunes, Ana Cristina Paulo, Catarina Candeias, Carina Valente, Alexandra S. Simões, Antonio Almeida Silva, Débora A. Tavares, António Brito‐Avô, Hermı́nia de Lencastre, Raquel Sá‐Leão,
In Portugal, the 13-valent pneumococcal conjugate vaccine (PCV13) was commercially available between 2010 and 2015, following a decade of private use of PCV7. We evaluated changes on serotype distribution and antimicrobial susceptibility of pneumococci carried by children living in two regions of Portugal (one urban and one rural). Three epidemiological periods were defined: pre-PCV13 (2009-2010), early-PCV13 (2011-2012), and late-PCV13 (2015-2016). Nasopharyngeal samples (n = 4,232) were obtained from ...
Tópico(s): Pneumocystis jirovecii pneumonia detection and treatment
2021 - Elsevier BV | Vaccine
Geneviève Garriss, Priyanka Nannapaneni, Alexandra S. Simões, Sarah Browall, Karthik Subramanian, Raquel Sá‐Leão, Herman Goossens, Hermı́nia de Lencastre, Birgitta Henriques‐Normark,
S. pseudopneumoniae is an overlooked pathogen emerging as the causative agent of lower-respiratory-tract infections and associated with chronic obstructive pulmonary disease (COPD) and exacerbation of COPD. However, much remains unknown on its clinical importance and epidemiology, mainly due to the lack of specific markers to distinguish it from S. pneumoniae . Here, we provide a new molecular marker entirely specific for S. pseudopneumoniae and offer a comprehensive view of the virulence and colonization ...
Tópico(s): Infective Endocarditis Diagnosis and Management
2019 - American Society for Microbiology | mBio
Produção Nacional
Revisado por pares
Raquel Sá‐Leão, Sofia C. Nunes, António Brito‐Avô, Nelson Frazão, Alexandra S. Simões, M. Inês Crisóstomo, Ana Cristina Paulo, Joana Saldanha, Ilda Santos‐Sanches, H. de Lencastre,
The seven-valent pneumococcal conjugate vaccine (PCV7) has been available in Portugal since June 2001, but is not included in the National Vaccination Plan. Its impact on colonization is unknown. A point-prevalence study to evaluate PCV7 usage was carried out in 2006 among day-care centre attendees from the Lisbon area. Pneumococcal carriage rates, serotypes, and antibiotypes were determined and compared with results from a similar study conducted in 2001 before vaccine approval. In 2001 and 2006, ...
Tópico(s): Respiratory viral infections research
2009 - Elsevier BV | Clinical Microbiology and Infection
Sónia Nunes, Sofia Félix, Carina Valente, Alexandra S. Simões, Débora A. Tavares, Antonio Almeida Silva, Ana Cristina Paulo, António Brito‐Avô, Hermı́nia de Lencastre, Raquel Sá‐Leão,
In Portugal, the 7-valent pneumococcal conjugate vaccine (PCV7) was not introduced in the national immunization plan but was commercially available between 2001 and 2010. We studied serotype distribution and antibiotic susceptibility of Streptococcus pneumoniae carried by children in 2009 and 2010. Vaccination with PCV7 was extracted from children's immunization bulletins and information on recent antimicrobial consumption was obtained through a questionnaire. For comparison, we included data from ...
Tópico(s): Pneumocystis jirovecii pneumonia detection and treatment
2016 - Elsevier BV | Vaccine
Alexandra S. Simões, Débora A. Tavares, Dora Rolo, Carmen Ardanuy, Herman Goossens, Birgitta Henriques‐Normark, Josefina Liñares, Hermı́nia de Lencastre, Raquel Sá‐Leão,
During surveillance studies we detected, among over 1500 presumptive pneumococci, 11 isolates displaying conflicting or novel results when characterized by widely accepted phenotypic (optochin susceptibility and bile solubility) and genotypic (lytA-BsaAI-RFLP and MLST) identification methods. We aimed to determine the genetic basis for the unexpected results given by lytA-BsaAI-RFLP and investigate the accuracy of the WHO recommended lytA real-time PCR assay to classify these 11 isolates. Three novel ...
Tópico(s): Bacterial Infections and Vaccines
2016 - Elsevier BV | Diagnostic Microbiology and Infectious Disease
Miguel Pinto, João Rodrigues, Rui Matias, Ivone Água‐Doce, Dora Cordeiro, Cristina Belo Correia, João Paulo Gomes, Maria José Borrego, Filipa Alegria, Ana Jacinta Piedade, Maria Beatriz Tomaz, Cristina Toscano, Mariana Pessanha, Eliana Costa, Agostinho Lira, Catarina Chaves, Ana Cristina Simões e Silva, Margarida Pinto, Manuela Ribeiro, Angélica Ramos, Hugo Cruz, Maria Helena Ramos, José Melo‐Cristino, Maria Inês Stilwell, Carlos Marques, Mariana Garcez, M. J. A. PEREIRA, Vera P. Santos, Marco Amaral, Maria José Rego de Sousa, Maria Favila Menezes, Elsa Calado, Luís Dias, Florbela Pereira, Idalina Rocha, Paula Mota Vieira, Maria Alberta Faustino, Maria Carmen Iglesias, R Pinto, L.A. de Francisco Serpa, Adriana Coutinho, Filomena Caldeira, José Diogo, Luísa Sancho, Sandra Schäfer, José Carlos Camisão, Graça Trigueiro, Alexandra Costa, Alda Campos, Alexandra Pereira, Ana Catarina Guerreiro, Gizela Santos, Mónica Cardoso, Filomena Lencastre, Luísa Frazão, Sofia Soares, Inês Figueiredo, Vitória Eduarda Silva Rodrigues, Eugénio Corrêa, Rui Campainha, Maria Rosário Barros, Sofia Botelho Moniz,
Tópico(s): Syphilis Diagnosis and Treatment
2020 - Springer Science+Business Media | European Journal of Clinical Microbiology & Infectious Diseases
Catarina Gouveia, Alexandra Gavino, Ons Bouchami, Maria Miragaia, Luís Varandas, Hermı́nia de Lencastre, Maria João Brito,
Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emerging public health problem worldwide. Severe invasive infections have been described, mostly associated with the presence of Panton-Valentine leukocidin (PVL). In Portugal limited information exists regarding CA-MRSA infections. In this study we describe the case of a previously healthy 12-year-old female, sport athlete, who presented to the hospital with acetabulofemoral septic arthritis, myositis, fasciitis, acetabulum ...
Tópico(s): Infective Endocarditis Diagnosis and Management
2013 - Hindawi Publishing Corporation | Case Reports in Pediatrics
Catarina Candeias, Antonio Almeida Silva, Ana Cristina Paulo, Alexandra S. Simões, Bárbara Ferreira, Ana Rita Cruz, Miguel Queirós, Tiago Touret, António Brito‐Avô, Hermı́nia de Lencastre, Raquel Sá‐Leão,
Streptococcus pneumoniae carriage studies are crucial to monitor changes induced by use of pneumococcal conjugate vaccines and inform vaccination policies. In this cross-sectional study, we examined changes within the pneumococcal population following introduction of PCV13 in 2015 in the National Immunization Program (NIP), in Portugal. In 2018-2020 (NIP-PCV13), we obtained 1450 nasopharyngeal samples from children ≤6 years attending day-care. We assessed serotypes, antimicrobial resistance, and genotypes ( ...
Tópico(s): Respiratory viral infections research
2024 - Elsevier BV | Vaccine
Nuno A. Faria, Tiago Touret, Alexandra S. Simões, Carlos Palos, Stephanie Bispo, José Melo‐Cristino, Mário Ramirez, João André Carriço, Margarida Pinto, Cristina Toscano, Elsa Gonçalves, Marta Gonçalves, Ana Roberta Fusco da Costa, Moacyr Araújo, Aida Duarte, Hermı́nia de Lencastre, Maria Elisa Serrano Navacerrada, Raquel Sá‐Leão, Maria Miragaia,
Carbapenem-resistant Klebsiella pneumoniae complex (CR-KP) are a public health concern, causing infections with a high mortality rate, limited therapeutic options and challenging infection control strategies. In Portugal CR-KP rate has increased steeply, but the factors associated to this expansion are poorly explored. To address this question we compared, by phylogenetic and resistome analysis, the draft genomes of 200 CR-KP isolates collected in 2017-2019 from five hospitals in the Lisbon region, ...
Tópico(s): Antibiotics Pharmacokinetics and Efficacy
2024 - Elsevier BV | Journal of Hospital Infection
Alexandra Amaro, Margarida Louro Felgueiras, Marina Prieto Afonso Lencastre,
As abordagens filosóficas conservacionista e protecionista atribuem o surgimento de inquietações sociais sobre os maus tratos infligidos aos animais não humanos em diversos países do ocidente, na transição do século XIX para o século XX. Este movimento social desafiou a visão hegemónica antropocêntrica, obteve a criação das primeiras áreas protegidas e organizou-se em associações de cidadãos, inquietos com o sofrimento animal, orientados para a proteção, prestação de assistência e defesa dos animais ...
Tópico(s): Environmental Sustainability and Education
1969 - Programa de Pós-graduação em Geografia (PPGEO) | Revista Tempos e Espaços em Educação