
Carlos Alberto Soriano-Souza, André L. Rossi, Elena Mavropoulos, Moema de Alencar Hausen, Marcelo N. Tanaka, Mônica Diuana Calasans‐Maia, José Mauro Granjeiro, Maria Helena M. Rocha‐Leão, Alexandre Malta Rossi,
Tópico(s): Advanced Drug Delivery Systems
2015 - Springer Science+Business Media | Journal of Materials Science Materials in Medicine
Margarida Fortuna, Maria João Sousa, Manuela Côrte‐Real, Cecı́lia Leão, Alexandre Salvador, Filipe Sansonetty,
Abstract Staining protocols generally designed for the flow cytometric analysis of the cell cycle in mammalian cells are frequently not satisfactory for quantification of the various cell‐cycle phases in yeasts. High CVs limit the accuracy of DNA content measurement and estimates of populations in cell‐cycle compartments. This unit describes a staining procedure for yeasts using the sensitive nucleic acid stain SYBR Green I, which binds to double‐stranded DNA with high selectivity and which has ...
Tópico(s): Bacteriophages and microbial interactions
2000 - Wiley | Current Protocols in Cytometry

Fernando Miranda de Vargas, Charles Ferreira Martins, Guilherme dos Santos Pinto, Marcos Barbosa Ferreira, Hélio de Almeida Ricardo, André Gustavo Leão, Alexandre Rodrigo Mendes Fernandes, A. Teixeira,
Tópico(s): Animal Nutrition and Physiology
2014 - Springer Science+Business Media | Tropical Animal Health and Production

André Melo, Maria J. Ramos, Wely B. Floriano, J.A.N.F. Gomes, J.F.R. Leão, Alexandre L. Magalhães, Bernard Maigret, Maria Clara Pinheiro do Nascimento, Nathalie Reuter,
The importance of the guanidinium–carboxylate interactions has sprung from the observed salt bridges often present in biological systems involving the arginine–glutamate or arginine–aspartate side chains. The strength of these interactions has been explained on the basis of a great coulombic energy gain, due to the closeness of two charges of opposite sign and the occurrence of H-bond interactions. However, in some environments proton transfer, from guanidinium to carboxylate, can occur with the ...
Tópico(s): Molecular spectroscopy and chirality
1999 - Elsevier BV | Journal of Molecular Structure THEOCHEM
Tiago Ribeiro, Filipa Lemos, Marco Preto, Joana Azevedo, Maria Lígia Sousa, Pedro N. Leão, Alexandre Campos, Stig Linder, Rui Vitorino, Vı́tor Vasconcelos, Ralph Urbatzka,
Portoamides are cyclic peptides produced and released by the cyanobacterial strain Phormidium sp. presumably to interfere with other organisms in their ecosystems ("allelopathy"). Portoamides were previously demonstrated to have an antiproliferative effect on human lung carcinoma cells, but the underlying mechanism of this activity has not been described. In the present work, the effects of portoamides on proliferation were examined in eight human cancer cell lines and two non-carcinogenic cell lines, ...
Tópico(s): bioluminescence and chemiluminescence research
2017 - Public Library of Science | PLoS ONE

Carlos Alberto Soriano-Souza, Helder Valiense, Elena Mavropoulos, Victor R. Martinez-Zelaya, Andréa Machado Costa, Adriana Terezinha Neves Novellino Alves, Mariana M. Longuinho, Rodrigo Figueiredo de Brito Resende, Carlos Fernando de Almeida Barros Mourão, José Mauro Granjeiro, Maria Helena M. Rocha‐Leão, Alexandre Malta Rossi, Mônica Diuana Calasans‐Maia,
Abstract Drug delivery technology is a promising way to enhance the therapeutic efficacy of drugs. The purpose of this study is to evaluate the physical and chemical properties of hydroxyapatite ceramic microspheres loaded with doxycycline (HADOX), their effects on in vitro osteoblast viability, and their antimicrobial activity, and to determine the effects of DOX on the healing of rat sockets after tooth extraction. The internal microsphere porosity was sensitive to the treatment used to adsorb ...
Tópico(s): Orthopedic Infections and Treatments
2019 - Wiley | Journal of Biomedical Materials Research Part B Applied Biomaterials

Juliana Munari Oliveira Pinto, Andrés Felipe Chamorro Rengifo, Cassiana Mendes, Aline Franciane Leão, Alexandre Luís Parize, Hellen Karine Stulzer,
Soluplus® (polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer) is a solubilizing copolymer commonly applied as carrier in solid dispersions of poorly soluble drugs. This polymer is used to increase the apparent solubility of drugs with low aqueous solubility and consequently enhance drug absorption by the human gastrointestinal tract. To select the appropriate carrier to compose solid dispersions, in vitro supersaturation studies were applied as a pre-formulation tool, using ...
Tópico(s): Pharmacological Effects of Natural Compounds
2019 - Elsevier BV | Colloids and Surfaces B Biointerfaces

Ana Patrícia Alves Leão, Alexandre Vinhas de Souza, Nelson Fijamo Mesquita, Luciano José Pereira, Márcio Gilberto Zangerônimo,
The purpose of this systematic review was to evaluate the potential benefits of antioxidant enrichment of semen extenders. These substances are used to combat oxidative stress during processing and conservation of rooster semen. A literature search was performed in June 2020 using the keywords rooster AND (semen OR spermatozoa OR sperm OR ejaculate OR ejaculation). This report followed the PRISMA (Preferred Reporting Items for Systematic Review and Meta-Analysis) guidelines. The PICO (population intervention ...
Tópico(s): Reproductive Biology and Fertility
2021 - Elsevier BV | Research in Veterinary Science

Rodrigo Abreu, Pedro Figueiredo, Paulo Beckert, José Pedro Marques, Samuel Amorim, Carlos Alberto Caetano, Pedro Carvalho, Carla Sá, Ricardo Cotovio, Joana Cruz, Tiago Dias, Gonçalo Fernandes, Elton Gonçalves, César Leão, Alexandre Leitão, João Lopes, Eduardo Machado, Monica Araujo das Neves, André Luiz de Oliveira, Ana Isabel Pereira, Bruno Pereira Pereira, Fernando Ribeiro, Luis M Silva, Filipe Antônio de Barros Sousa, Tânia Tinoco, Vítor Hugo Teixeira, Mónica Sousa, João Brito,
Nutrition is an undeniable part of promoting health and performance among football (soccer) players. Nevertheless, nutritional strategies adopted in elite football can vary significantly depending on culture, habit and practical constraints and might not always be supported by scientific evidence. Therefore, a group of 28 Portuguese experts on sports nutrition, sports science and sports medicine sought to discuss current practices in the elite football landscape and review the existing evidence on ...
Tópico(s): Exercise and Physiological Responses
2021 - BMJ | BMJ Open Sport & Exercise Medicine

Luís Henrique da Costa Leão, Alexandre de Carvalho Castro,
A criação da Rede Nacional de Atenção Integral à Saúde do Trabalhador (Renast), em 2002, fez surgir e ampliar o número de Centros de Referência em Saúde do Trabalhador (Cerest) no Brasil. Na Região Norte Fluminense, o Cerest foi inaugurado em 2006 e resultou da transformação do antigo Programa de Saúde do Trabalhador (PST) da cidade de Campos dos Goytacazes. Esta pesquisa analisa, sob o horizonte mais amplo da história dos Programas de Saúde do Trabalhador no Brasil e do surgimento da Renast, a implantação ...
Tópico(s): Youth, Drugs, and Violence
2013 - ASSOCIAÇÃO BRASILEIRA DE SAÚDE COLETIVA | Ciência & Saúde Coletiva

Julcéia Camillo, André Pereira Leão, Alexandre Alonso Alves, Eduardo F. Formighieri, Ana Luisa Sousa Azevedo, Juliana D. Nunes, Guy de Capdeville, J. K. A. Mattos, Manoel Teixeira Souza,
Aiming at generating a comprehensive genomic database on Elaeis spp., our group is leading several R&D initiatives with Elaeis guineensis (African oil palm) and Elaeis oleifera (American oil palm), including the whole-genome sequencing of the last. Genome size estimates currently available for this genus are controversial, as they indicate that American oil palm genome is about half the size of the African oil palm genome and that the genome of the interspecific hybrid is bigger than both the ...
Tópico(s): Chromosomal and Genetic Variations
2014 - SAGE Publishing | Genomics Insights

Alexandre Leão Quadro, José Roberto Tavares Branco,
Drilling tests have been carried out to characterize the performance of cutting materials, hard coatings and for characterization of lubricants. In the search for enhanced productivity there is interest in monitoring the wear process of cutting tools in real time. With this application in mind, acoustic emission during drilling has been investigated. Drills of high speed steel, with and without TiN coatings, were used and AISI D3 was the working material. The acoustic emission in the range of 100– ...
Tópico(s): Hydrogen embrittlement and corrosion behaviors in metals
1997 - Elsevier BV | Surface and Coatings Technology

Jones Remo Barbosa Vale, Carlos Alexandre Leão Bordalo,
A bacia hidrográfica é considerada uma unidade ambiental, pois permite a análise de seus diversos componentes e processos dentro de uma abordagem sistêmica. A bacia hidrográfica do rio Apeú está localizada na porção oriental da Amazônia, mais precisamente no nordeste paraense, e sua área abrange os municípios de Castanhal, Santa Izabel do Pará e Inhangapí. O trabalho tem por objetivo fazer uma caracterização da morfometria e do uso e cobertura da Terra da bacia do rio Apeú, a fim de subsidiar o ...
Tópico(s): Fish biology, ecology, and behavior
2020 - | Formação (Online)

Carlos Alexandre Leão Bordalo,
Neste início de século, o Conselho Mundial da Água já organizou cinco Fóruns Mundiais da Água, em que se apresentaram dados de uma crise eminente no abastecimento de água doce. Tema também apresentado pela ONU e sobre o qual todos devemos refletir. Aqui, apresentamos uma reflexão sobre o paradoxo da água na Amazônia brasileira, e veremos que, estando ela no trópico úmido e tendo a maior disponibilidade hídrica superficial, atmosférica e subterrânea do país, não há ali propriamente uma crise de disponibilidade ...
Tópico(s): Environmental Sustainability and Education
2017 - UNIVERSIDADE DE SÃO PAULO | GEOUSP Espaço e Tempo (Online)

Karen Cristina Pires da Costa, José Francisco de Carvalho Gonçalves, Alexandre Leão Gonçalves, Adamir da Rocha Nina, Roberto Kirmayr Jaquetti, Vinícius Fernandes de Souza, Josiane Celerino de Carvalho, Andréia Varmes Fernandes, Joelma Keith Rodrigues, Gleisson de Oliveira Nascimento, L. H. de O. Wadt, Karen A. Kainer, R. M. B. de Lima, Flávia Camila Schimpl, Jéssica Pereira de Souza, Sabrina Silva de Oliveira, Hellen Thaís da Silva Miléo, Diego P. Souza, Ana Claudia Lopes da Silva, Heloisa Massaco Ito Nascimento, Jair Max Furtunato Maia, Francisco de Almeida Lobo, Paulo Mazzafera, Márcio V. Ramos, Héctor H. F. Koolen, R. R. de Morais, Karina Martins, Niwton Leal Filho, Henrique Eduardo Mendonça Nascimento, Katharine Duarte Gonçalves, Yasmin Verçosa Kramer, Giordane Martins, Marcelo O. Rodrigues,
Tópico(s): Plant and animal studies
2022 - Springer Science+Business Media | Current Forestry Reports

Luciana Helena Maia Porte, Maria Helena Miguez Rocha Leão, Alexandre Porte,
MICROCAPSULES CONTAINING BIOCATIVE PROTEIN BY MERCURY POROSIMETRY AND NITROGEN ADSORPTION.Microcapsules containing lactoferrin were produced by spray drying using dextrin:octenylsuccinate starch, as wall materials.Porosity characteristics of spray-dried microcapsules were investigated by mercury intrusion porosimetry and nitrogen adsorption.The outer and inner structures of microcapsules were studied by Scanning Electron Microscopy and sizes were determined by Laser Diffraction.Results indicate ...
Tópico(s): Electrospun Nanofibers in Biomedical Applications
2011 - Brazilian Chemical Society | Química Nova
Rui Bernardino, Rashid K. Sayyid, Ricardo Leão, Alexandre R. Zlotta, Theodorus van der Kwast, Laurence Klotz, Neil Fleshner,
The interest in broadening the application of active surveillance (AS) has been increasing, encompassing patients who may not strictly adhere to the conventional criteria for low-risk prostate cancer (PCa), particularly those diagnosed with small-volume Gleason grade group 2 disease. Nonetheless, accurately identifying individuals with low-intermediate risk PCa who can safely undergo AS without facing disease progression remains a challenge. This review aims to delve into the progression of this evolving ...
Tópico(s): Global Cancer Incidence and Screening
2023 - | Canadian Urological Association Journal

Gustavo José Braga, Alexandre Carneiro Leão de Mello, Carlos Guilherme Silveira Pedreira, Henrique Rocha de Medeiros,
O objetivo deste trabalho foi avaliar o efeito da intensidade de pastejo na taxa diária de acúmulo de forragem e na fotossíntese foliar líquida do capim-tanzânia, em pastagens manejadas sob lotação intermitente. O experimento foi conduzido de novembro de 1999 a outubro de 2000, em delineamento de blocos ao acaso, com quatro repetições. Os tratamentos experimentais foram três quantidades de massa de forragem verde remanescente (1.000, 2.500 e 4.000 kg ha -1), aplicados em ciclos de pastejo de 36 dias ( ...
Tópico(s): Agroforestry and silvopastoral systems
2009 - Embrapa Informação Tecnológica | Pesquisa Agropecuária Brasileira

Fabiana Lopes de Paula, Isabela Cerqueira Barreto, Maria Helena M. Rocha‐Leão, Radovan Borojević, Alexandre Malta Rossi, Fabiana Paim Rosa, Marcos Farina,
Tópico(s): Bone health and osteoporosis research
2009 - Springer Nature | Frontiers of Materials Science in China

Alexandre Carneiro Leão de Mello, M. A. Lira, José Carlos Batista Dubeux, Mércia Virgínia Ferreira dos Santos, Erinaldo Viana de Freitas,
O estudo visou caracterizar uma coleção de 71 clones de capim-elefante e selecionar os mais promissores para serem testados sob pastejo. A caracterização foi realizada na Estação Experimental do Cedro/IPA, no município de Vitória de Sto. Antão/PE, nas estações chuvosa e seca. Cada clone foi representado por parcelas não repetidas de 10 m². Segundo análise de correlação linear, os clones mais produtivos em relação à MS, também apresentaram maiores alturas de planta e de meristemas apicais, diâmetros ...
Tópico(s): Forest ecology and management
2002 - Sociedade Brasileira de Zootecnia | Revista Brasileira de Zootecnia

Mércia Virgínia Ferreira dos Santos, José Carlos Batista Dubeux, Maria da Conceição Silva, Stuart Francisco dos Santos, Rinaldo Luíz Caraciolo Ferreira, Alexandre Carneiro Leão de Mello, Iderval Farias, Erinaldo Viana de Freitas,
O trabalho foi realizado com o objetivo de avaliar o potencial produtivo e a composição química de cinco gramíneas tropicais (Pennisetum purpureum Schum. cv. Pioneiro, P. purpureum Schum cv. Mott; Panicum maximum Jacq. cv. Mombaça, P. maximum Jacq cv. Tanzânia e Brachiaria brizantha (Hochst) Stapf. cv. Marandu). Os cortes foram realizados em intervalos de 35 dias e a 40 cm do solo. O cultivar Pioneiro apresentou maior produção de MS (7,35 t/ha/35 dias), quando comparado aos cultivares Mott (5,28 t/ha/ ...
Tópico(s): Agroforestry and silvopastoral systems
2003 - Sociedade Brasileira de Zootecnia | Revista Brasileira de Zootecnia

Valfrido Leão de Melo Neto, Alexandre Martins Valença, Isabella Nascimento, Fabiana L. Lopes, Antônio Egídio Nardi,
BACKGROUND: Panic disorder (PD) patients show more physical and psychological impairment, than the general population. OBJECTIVES: Our aims are to compare quality of life between PD patients during treatment and healthy subjects and to associate social, demographic and clinical factors with quality of life scores. METHODS: It is a cross-sectional study with 20 PD patients and 20 healthy controls. Anxiety levels assessment: Beck Anxiety Inventory, Hamilton Anxiety Rating Scale, STAIT - Form Y. Panic ...
Tópico(s): Maternal Mental Health During Pregnancy and Postpartum
2008 - UNIVERSIDADE DE SÃO PAULO | Archives of Clinical Psychiatry (São Paulo)

Nalígia Gomes de Miranda e Silva, M. A. Lira, Mércia Virgínia Ferreira dos Santos, José Carlos Batista Dubeux, Alexandre Carneiro Leão de Mello, Maria da Conceição Silva,
Objetivou-se avaliar a relação entre as características morfológicas e produtivas de clones de palma-forrageira para identificação daqueles que contribuem diretamente na produção. Foram avaliados 50 clones de palma-forrageira estabelecidos no palmal há cinco anos e submetidos ao primeiro corte dois anos antes da avaliação. Foram avaliadas as características da planta e do cladódio, e os dados foram analisados pela estatística descritiva, correlação de Pearson e pela análise de trilha das variáveis- ...
Tópico(s): Agricultural pest management studies
2010 - Sociedade Brasileira de Zootecnia | Revista Brasileira de Zootecnia

Mércia Virgínia Ferreira dos Santos, M. A. Lira, José Carlos Batista Dubeux, Adriana Guim, Alexandre Carneiro Leão de Mello, Márcio Vieira da Cunha,
Caatinga is the most important biome for the livestock in the Brazilian semi-arid region. This review paper aimed to present information on different forage aspects of caatinga vegetation for ruminant feeding. Caatinga vegetation is formed mainly by shrubs and small trees, usually presenting thorns, deciduous leaves, and leaf abscission occurring frequently at the onset of the dry season. Additional components of the botanical composition in this biome includes the families cactaceae, bromeliaceae, ...
Tópico(s): Environmental and biological studies
2010 - Sociedade Brasileira de Zootecnia | Revista Brasileira de Zootecnia

André L. Rossi, Isabela Cerqueira Barreto, William Q. Maciel, Fabiana Paim Rosa, Maria Helena M. Rocha‐Leão, J. Werckmann, Alexandre Malta Rossi, Radovan Borojević, Marcos Farina,
We report the ultrastructure of regenerated bone surrounding two types of biomaterials: hydroxyapatite-alginate composite and sintered hydroxyapatite. Critical defects in the calvaria of Wistar rats were filled with micrometer-sized spherical biomaterials and analyzed after 90 and 120 days of implantation by high-resolution transmission electron microscopy and Fourier transform infrared attenuated total reflectance microscopy, respectively. Infrared spectroscopy showed that hydroxyapatite of both ...
Tópico(s): Facial Trauma and Fracture Management
2011 - Elsevier BV | Bone

Daniel Fernando Ydoyaga Santana, M. A. Lira, Mércia Virgínia Ferreira dos Santos, Marcelo de Andrade Ferreira, Maria José de Araújo Silva, Kleyton Alcantara Marques, Alexandre Carneiro Leão de Mello, Djalma Cordeiro dos Santos,
Um experimento foi conduzido para caracterizar uma caatinga manipulada e estimar a composição botânica e qualidade da dieta de novilhos fistulados. O experimento foi conduzido no período chuvoso, entre março e julho de 2003. A caatinga foi pastejada por 24 novilhas das raças Guzerá e Girolando e dois novilhos esôfago-fistulados. Na caatinga, foram identificados 24 famílias, 38 gêneros e 41 espécies nos dois estratos estudados (dessas, dez espécies foram encontradas na dieta dos animais). As massas ...
Tópico(s): Moringa oleifera research and applications
2011 - Sociedade Brasileira de Zootecnia | Revista Brasileira de Zootecnia

Márcio Vieira da Cunha, M. A. Lira, Mércia Virgínia Ferreira dos Santos, Erinaldo Viana de Freitas, José Carlos Batista Dubeux, Alexandre Carneiro Leão de Mello, Kalina Gerciane Rodovalho Martins,
The objectives in this work were to study the association between the morphological and productive characteristics in Pennisetum sp. clones, and to identify the morphological characteristics responsible for the productivity in Pennisetum cp. clones. The canonical correlations were evaluated and the path analysis was made from the simple genotypic correlation matrix between the morphological and productive characteristics of eight Pennisetum sp. clones (Taiwan A-146 2.37, Taiwan A-146 2.27, Taiwan- ...
Tópico(s): Turfgrass Adaptation and Management
2011 - Sociedade Brasileira de Zootecnia | Revista Brasileira de Zootecnia

Clebson Sidney Sabino. Lima, Marta Maria da Conceição, Flávio L.H. Silva, Ezenildo Emanuel de. Lima, Líbia de S Conrado, Douglas Alexandre Saraiva Leão,
The present study aimed to characterize and evaluate the hydrolysis of sisal fibers for ethanol production. Acid hydrolysis of the fiber was performed and main products determined were xylose and glucose. However, xylose and arabinose (pentoses) are not fermented by the conventional yeast Saccharomyces cerevisiae. Prehydrolysis at 105 °C was efficient, removing most of the pentoses. The main product formed in acid hydrolysis at 160 °C was glucose, which can be metabolized by the yeast S. cerevisiae ...
Tópico(s): Biofuel production and bioconversion
2012 - Elsevier BV | Applied Energy

Antônio Egídio Nardi, Isabella Nascimento, Rafael C. Freire, Valfrido Leão de Melo Neto, Alexandre Martins Valença, Mônica Dib, Gastão L. Soares‐Filho, André Barciela Veras, Marco A. Mezzasalma, Fabiana L. Lopes, Gabriela B. de Menezes, Leila O. Grivet, Márcio Versiani,
Schizobipolar disorder is considered related to both schizophrenia and bipolar disorder. We aimed to describe with retrospective methodology the demographic, clinical, and treatment features in a group of schizobipolar disorder patients who have been treated for at least a 5-year period and compare them with bipolar I and schizophrenic patients who were treated during the same period. We compared the demographic and clinical data of 61 schizobipolar, 57 bipolar I, and 55 schizophrenic outpatients ...
Tópico(s): Schizophrenia research and treatment
2005 - Elsevier BV | Journal of Affective Disorders

Alexandre Carneiro Leão de Mello, Carlos Guilherme Silveira Pedreira,
Objetivando quantificar respostas morfológicas de dosséis de capim-Tanzânia (Panicum maximum Jacq. cv. Tanzânia-1) sob três intensidades de pastejo, lotação rotacionada e irrigação, foi conduzido um experimento em delineamento experimental de blocos completos casualizados com quatro repetições. Os tratamentos foram três intensidades de pastejo, representados pelas quantidades de massa seca verde residual pós-pastejo (T1=1000; T2=2500 e T3=4000 kg MSV/ha). Durante oito ciclos de pastejo (rebrotas de ...
Tópico(s): Soil Management and Crop Yield
2004 - Sociedade Brasileira de Zootecnia | Revista Brasileira de Zootecnia