Jonathan Weitz, Hervé Tiriac, Tatiana Hurtado de Mendoza, Alexis A. Wascher, Andrew M. Lowy,
Organotypic tissue slices prepared from patient tumors are a semi-intact ex vivo preparation that recapitulates many aspects of the tumor microenvironment (TME). While connections to the vasculature and nervous system are severed, the integral functional elements of the tumor remain intact for many days during the slice culture. During this window of time, the slice platforms offer a suite of molecular, biomechanical and functional tools to investigate PDAC biology. In this review, we first briefly ...
Tópico(s): 3D Printing in Biomedical Research
2021 - Multidisciplinary Digital Publishing Institute | Cancers
Sandeep Vellanki, María Isabel Navarro-Mendoza, Alexis Garcia, Laura Murcia, Carlos Pérez‐Arques, Victoriano Garre, Francisco E. Nicolás, Soo Chan Lee,
Mucor circinelloides is a fungus that belongs to the order Mucorales. It grows as mold in the environment and can cause mucormycosis, a potentially fatal infection in immunocompromised patients. M. circinelloides is a biodiesel producer and serves as a model organism for studying several biological processes, such as light responses and RNA interference-mediated gene silencing. Over the past decade, the increasing number of molecular tools has also allowed us to manipulate the genome of this fungus. ...
Tópico(s): Microbial Natural Products and Biosynthesis
2018 - Wiley | Current Protocols in Microbiology
José Miguel Moreno-Ortíz, Melva Gutiérrez‐Angulo, Miriam Partida-Pérez, Jorge Peregrina-Sandoval, Ruth Ramírez-Ramírez, R. Muñiz-Mendoza, Alexis Sayuri Suárez-Villanueva, M. Centeno-Flores, Víctor Maciel-Gutiérrez, J.E. Cabrales-Vazquez, María de la Luz Ayala-Madrigal,
several metalloproteinases (MMPs), including MMP13 and MMP7, which play an important role in tumor ©FUNPEC-RP www.funpecrp.com.brGenetics and Molecular Research 13 (2): 3537-3544 (2014) invasion and metastasis.The objective of this study was to analyze the association of functional MMP7-181A/G and MMP13-77A/G promoter polymorphisms with susceptibility to CRC in a Mexican population.Genomic DNA samples were obtained from peripheral blood of 102 CRC patients and 125 blood donors who were included as the ...
Tópico(s): Peptidase Inhibition and Analysis
2014 - Research Foundation of Ribeirão Preto | Genetics and Molecular Research
Juan José Cabello Eras, Jorge Mario Mendoza Fandiño, Alexis Sagastume Gutiérrez, Juan Gabriel Rueda-Bayona, Stiven Javier Sofán German,
The inequality in electricity consumption (EC) in Colombia is assessed based on the per capita electricity consumption (ECpc) and the Gini coefficient. The ECpc was defined with the total electricity consumption and population for each department and rural and urban areas. Furthermore, the national ECpc was assessed according to the Colombian socioeconomic stratum defined by income levels. The results showed significant asymmetries in the ECpc between departments, urban and rural populations, and ...
Tópico(s): Smart Grid Energy Management
2022 - Elsevier BV | Energy
Isaura Bañuelos-Vargas, Gustavo Alejandro Rodríguez‐Montes de, Emmanuel Martínez‐Montaño, Amalia Pérez‐Jiménez, Oscar Alexis Mendoza-Gamboa, José Antonio Estrada-Godínez, Crisantema Hernández,
Tópico(s): Aquatic life and conservation
2021 - Elsevier BV | Aquaculture
Eduardo De-La-Hoz-Correa, Fabio Mendoza Palechor, Alexis De-La-Hoz-Manotas, Roberto Cesar Morales-Ortega, Sánchez Hernández Beatriz Adriana,
Obesity has become a global epidemic that has doubled since 1980, with serious consequences for health in children, teenagers and adults. Obesity is a problem has been growing steadily and that is why every day appear new studies involving children obesity, especially those looking for influence factors and how to predict emergence of the condition under these factors. In this study, authors applied the SEMMA data mining methodology, to select, explore and model the data set and then three methods ...
Tópico(s): Artificial Intelligence in Healthcare
2019 - Science Publications | Journal of Computer Science
Jacinto Elías Sedeño-Díaz, Eugenia López‐López, Erick Mendoza-Martínez, Alexis Joseph Rodríguez-Romero, S. S. Morales-García,
The Lake of Texcoco is a closed basin with soils that confer salinity, conductivity, and alkalinity to it. It has undergone a reduction in size, incorporation of wastewater, and short-term desiccation, and includes temporary wetlands interconnected during the rainy season, some of which receive treated wastewater. Sediments contain metals, thus affecting water quality. Five artificial lakes were studied, and 12 metals (As, Ba, Cd, Cu, Cr, Fe, Mg, Mn, Hg, Ni, Pb, and Zn) were monitored bimonthly in ...
Tópico(s): Geochemistry and Geologic Mapping
2019 - Multidisciplinary Digital Publishing Institute | Water
Fabio Mendoza Palechor, Alexis De la Hoz Manotas,
This paper presents data for the estimation of obesity levels in individuals from the countries of Mexico, Peru and Colombia, based on their eating habits and physical condition. The data contains 17 attributes and 2111 records, the records are labeled with the class variable NObesity (Obesity Level), that allows classification of the data using the values of Insufficient Weight, Normal Weight, Overweight Level I, Overweight Level II, Obesity Type I, Obesity Type II and Obesity Type III. 77% of the ...
Tópico(s): Data Mining Algorithms and Applications
2019 - Elsevier BV | Data in Brief
Alexis Mendoza-Ruiz, Suela Dylgjeri, F. Bour, Frédéric Damagnez, Karl Leroux, Sonia Khirani,
Only a few studies have addressed strategies to improve continuous positive airway pressure (CPAP) adherence in children with obstructive sleep apnea. The aim of our study was to assess the efficacy of a table based on token economy to improve CPAP adherence.A table was proposed to children nonadherent to CPAP (<3 h per night of CPAP use), eight days after CPAP initiation (D8). The child has to fill the table on a daily basis with green (I used my CPAP this night) or red (I did not use my CPAP) tokens. ...
Tópico(s): Cardiovascular Syncope and Autonomic Disorders
2018 - Elsevier BV | Sleep Medicine
Michel F. Martínez‐Reséndez, Elvira Garza‐González, Soraya Mendoza-Olazarán, Alexis Herrera-Guerra, Juan Rodríguez-López, Edelmiro Pérez‐Rodríguez, Roberto Mercado-Longoría, Adrián Camacho-Ortíz,
Up to 25% of all nosocomial infections (NIs) develop in critically ill patients. Our objective was to evaluate chlorhexidine (CHX) bathing and hand hygiene (HH) compliance in the reduction of NIs in the intensive care unit.The study comprised three 6-month periods: preintervention (PIP; soap/water bathing), intervention (IP; bathing with CHX-impregnated wipes), and postintervention (PoIP; soap/water bathing). An HH program was implemented during the IP and PoIP. Primary outcomes were global and specific ...
Tópico(s): Antibiotic Use and Resistance
2014 - Elsevier BV | American Journal of Infection Control
Luís Luís, María Luisa Serrano, Manuel Hidalgo, Alexis Mendoza-León,
Differential susceptibility to microtubule agents has been demonstrated between mammalian cells and kinetoplastid organisms such as Leishmania spp . and Trypanosoma spp . The aims of this study were to identify and characterize the architecture of the putative colchicine binding site of Leishmania spp . and investigate the molecular basis of colchicine resistance. We cloned and sequenced the β -tubulin gene of Leishmania (Viannia) guyanensis and established the theoretical 3D model of the protein, using ...
Tópico(s): Biochemical and Molecular Research
2013 - Hindawi Publishing Corporation | BioMed Research International
Xenón Serrano-Martín, Gilberto Payares, Marisel De Lucca, Juan Carlos Martı́nez, Alexis Mendoza-León, Gustavo Benaím,
Leishmaniasis is parasitic disease that is an important problem of public health worldwide. Intramuscularly administered glucantime and pentostam are the most common drugs used for treatment of this disease, but they have significant limitations due to toxicity and increasing resistance. A recent breakthrough has been the introduction of orally administered miltefosine for the treatment of visceral, cutaneous, and mucocutaneous leishmaniasis, but the relative high cost and concerns about teratogenicity ...
Tópico(s): Synthesis and Biological Evaluation
2009 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Herakles A. García, María Elena García, G. Pérez, Angélica Bethencourt, É. Zerpa, H Pérez, Alexis Mendoza-León,
The seroprevalence of trypanosomiasis and the prevalence of current trypanosome infection in water buffaloes from the most important livestock areas of Venezuela were evaluated by IFAT and the microhaematocrit centrifugation technique, respectively. The usefulness of a PCR-based assay for identifying the trypanosome species in the buffaloes was also evaluated. Of the 644 animals investigated, 40 (6.2%) were found infected with trypanosomes by blood centrifugation, and 196 (30.4%) were found positive ...
Tópico(s): Vector-borne infectious diseases
2006 - Maney Publishing | Annals of Tropical Medicine and Parasitology
Xenón Serrano-Martín, Gilberto Payares, Alexis Mendoza-León,
ABSTRACT Glibenclamide reduced the rate of lesion growth in BALB/c mice infected with Leishmania ( Leishmania ) mexicana , the effect was dose dependent, and the highest dose proved more effective than glucantime. Cross-resistance to glucantime was found in animals infected with a glibenclamide-resistant line, but combined therapy reduced lesion progression even in the glibenclamide-resistant strain.
Tópico(s): Synthesis and Biological Evaluation
2006 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Herakles A. García, María-E. García, H Pérez, Alexis Mendoza-León,
The usefulness of PCR-based assays for detecting trypanosomiasis in water buffaloes and other livestock was explored, under field conditions, in Venezuela. The sensitivity and specificity of the assays, which were based on established primer pairs (21-mer/22-mer and ILO1264/ILO1265), were evaluated, partly by comparison with the results of parasitological tests (stained bloodsmears and microhaematocrit centrifugation) and immunological assays (IFAT) run in parallel. The optimised PCR-based assays showed ...
Tópico(s): Vector-borne infectious diseases
2005 - Maney Publishing | Annals of Tropical Medicine and Parasitology
N. F. Garcia, Katherine Figarella, Alexis Mendoza-León, Alicia Ponte‐Sucre,
Tópico(s): Drug Transport and Resistance Mechanisms
2000 - Springer Science+Business Media | Parasitology Research
Milagros Postigo, Alexis Mendoza-León, Hilda Pérez,
A polymerase chain reaction (PCR) method that amplifies genus- and species-specific sequences present within the small subunit of ribosomal ribonucleic acid (ssRNA) genes of the human malaria parasites was used for the diagnosis of malaria in south-eastern Venezuela. One hundred blood samples were submitted to deoxyribonucleic acid extraction, PCR amplification and electrophoretic analysis of the PCR products, and the results were compared to those of routine microscopical diagnosis. The sensitivity ...
Tópico(s): Pharmaceutical Quality and Counterfeiting
1998 - Oxford University Press | Transactions of the Royal Society of Tropical Medicine and Hygiene
Alicia Ponte‐Sucre, Yelitza Campos, M. Cristina Fernández, Heidrun Moll, Alexis Mendoza-León,
In the present work we examined the effect of ion transport blockers on the growth and viability ofLeishmania sp.and on the infection of macrophages by the parasite. 4-aminopyridine and glibenclamide block voltage-dependent and K+ATP channels, respectively; amiloride is used to detect Na+channels and Na+/H+antiporters; and anthracene-9-carboxylic acid affects chloride channels. The EC50for promastigote cultures of three strains of theLeishmaniasubgenus, namely,Leishmania (Leishmania)NR,Leishmania ( ...
Tópico(s): Trypanosoma species research and implications
1998 - Elsevier BV | Experimental Parasitology
Luís Luís, Alvaro Ramı́rez, Cruz Manuel Aguilar, Salih Eresh, Douglas C. Barker, Alexis Mendoza-León,
We have demonstrated the polymorphism of the β-tubulin gene region in Leishmania and its value in the identification of the parasite. In this work we have shown that the coding region of the gene has sufficient variation to accurately discriminate these parasites at the subgenus level. Nevertheless, intrasubgenus diversity, for particular restriction enzymes, was found in New World Leishmania belonging to the Leishmania subgenus. For instance, differences were found between mexicana and amazonensis ...
Tópico(s): Enzyme function and inhibition
1998 - Elsevier BV | Acta Tropica
Alicia Ponte‐Sucre, Yelitza Campos, Jorge Vázquez, Heidrun Moll, Alexis Mendoza-León,
We have demonstrated that Leishmania spp. grown as promastigotes, are sensitive to the K+ channel inhibitors 4-aminopyridine and glibenclamide. Their host cells, the macrophages, are not affected by similar concentrations of the drugs. We have also initiated the molecular characterization of the mechanisms involved in the development of drug resistance to glibenclamide by the parasite. Therefore, we have selected experimentally and begun to characterize the Venezuelan Leishmania (Leishmania) strain, ...
Tópico(s): Trypanosoma species research and implications
1997 - Instituto Oswaldo Cruz, Ministério da Saúde | Memórias do Instituto Oswaldo Cruz
Alexis Mendoza-León, J C Havercroft, Douglas C. Barker,
SUMMARY We have examined the similarities and differences in the organization of tubulin genes in New World Leishmania by restriction endonuclease digestion of genomic DNA and Southern blot analysis, using heterologous and homologous tubulin gene probes. As judged by the hybridization pattern and the restriction fragment length polymorphism (RFLP), there were large differences in both the restriction and hybridization patterns of the β-tubulin sequences between stocks of the mexicana and braziliensis ...
Tópico(s): Fungal Plant Pathogen Control
1995 - Cambridge University Press | Parasitology
Salih Eresh, Maarten H.L. de Bruijn, J.Alexis Mendoza-León, Douglas C. Barker,
Leishmania lainsoni has recently been recognized as a new peripylarian species belonging to the subgenus Viannia and the L. braziliensis complex. It has been isolated from its sandfly vector, reservoir host and cutaneous lesions of human patients. Microscopical examination has shown characteristics which are different from those of other members of the L. braziliensis complex. Nuclear deoxyribonucleic acid (DNA) hybridization patterns with a beta tubulin probe and kinetoplast DNA buoyant density ...
Tópico(s): Trypanosoma species research and implications
1995 - Oxford University Press | Transactions of the Royal Society of Tropical Medicine and Hygiene
Ivan Sipiran, Alexis Mendoza, Alexander Apaza, Cristian Lopez,
Tópico(s): Image Processing and 3D Reconstruction
2022 - Springer Science+Business Media | International Journal of Computer Vision
Roberto GarcÃa-Alzate, Daisy Lozano-Arias, Rafael M. Reyes-Lugo, Antonio Morocoima, Leidi Herrera, Alexis Mendoza-Leà n,
Triatoma maculata is a wild vector of Trypanosoma cruzi, the causative agent of Chagas disease; its incursion in the domestic habitat is scant. In order to establish the possible domestic habitat of T. maculata, we evaluated wing variability and polymorphism of genotypic markers in subpopulations of T. maculata that live in different habitats in Venezuela. As markers, we used the mtCyt b gene, previously apply to evaluate population genetic structure in triatomine species, and the β-tubulin gene ...
Tópico(s): Insect symbiosis and bacterial influences
2014 - Frontiers Media | Frontiers in Public Health
Alcides Rafael Daza Daza, Ciro A. Serna-Mendoza, Alexis Carabalí-Angola,
El estudio tuvo como finalidad analizar mediante la implementación de herramientas cualitativas de investigación las condiciones de higiene, aseo y disposición del recurso agua en las comunidades indígenas de La Guajira Colombiana. Se identificó en las zonas analizadas las concepciones que la población tiene con relación al agua y el saneamiento básico dentro de sus territorios. Las apreciaciones que la población presentó con relación a la calidad del agua estuvieron condicionadas por los factores ...
Tópico(s): Public Health and Environmental Issues
2018 - Centro de Informacion Tecnologica | Información tecnológica
Ana Brito Gamboa, Mireya Mendoza, Alexis Fernández, Elvia Díaz,
Las levaduras de Candida causan el 80% de las infecciones nosocomiales por hongos. En 1995, se describió una nueva especie, Candida dubliniensis, que comparte con Candida albicans características morfológicas y fisiológicas. Estudiamos 109 aislamientos identificados como C. albicans, para detectar la presencia de C. dubliniensis, por estudios microbiológicos y PCR con cebadores DUBR/DUBF. Las pruebas microbiológicas resultaron positivas entre 90-98%. Dos de los 80 ADN estudiados (2,5%) mostraron ...
Tópico(s): Fungal Infections and Studies
2006 - Elsevier BV | Revista Iberoamericana de Micología
Produção Nacional
Revisado por pares
Alexis Mendoza-León, Luís Luís, Octávio Fernandes, Elisa Cupolillo, Lineth García,
We have previously identified a novel genomic sequence of 500 bp, the β500-DNA sequence, in the subgenus Leishmania (Viannia). This sequence was localized upstream of the β-tubulin gene. Restriction fragment length polymorphism and hybridization analysis has shown that the β500-DNA sequence is specific to this subgenus. A polymerase chain reaction (PCR) assay confirmed this specificity. The β500-DNA sequence was apparently absent from the genomic deoxyribonucleic acid of L. colombiensis and L. equatoriensis. ...
Tópico(s): Trypanosoma species research and implications
2002 - Oxford University Press | Transactions of the Royal Society of Tropical Medicine and Hygiene
Nieves Santos, Jorge Aguiar, Julio R. Fernández, María Vázquez, G Auburger, Suzana Gispert, Yssel Mendoza‐Marí, J. Alexis De Abreu Garcia, Luís Velázquez,
Tópico(s): Metabolism and Genetic Disorders
1999 - Elfos Scientiae | Biotecnología aplicada
Karina Mendoza‐Macedo, Alexis J. Romero-Díaz, Mariana P. Miranda‐Hernández, Víctor R. Campos‐García, Nancy D. Ramírez-Ibáñez, L. Carmina Juárez-Bayardo, Karen Moreno-Duran, Miriam S. Cedillo-Robles, Néstor O. Pérez, Helgi Jung–Cook, Luis F. Flores‐Ortiz, Emilio Medina‐Rivero,
Developing countries have an estimate of ten times more approved biosimilars than developed countries. This disparity demands the need of an objective regulation that incorporates health policies according to the technological and economical capabilities of each country. One of the challenges lies on the establishment of comparability principles based on a physicochemical and biological characterization that should determine the extent of additional non-clinical and clinical studies. This is particularly ...
Tópico(s): Pharmaceutical Economics and Policy
2016 - Elsevier BV | Electronic Journal of Biotechnology
Salih Eresh, Alexis Mendoza-León, Douglas C. Barker,
Orthogonal Field Alternating Gel Electrophoresis (OFAGE) has been used to show a band of approximately 260 kb which is stained intensely with ethidium bromide in Leishmania (V.) braziliensis stock M2903. This small chromosome (sc-2903), as well as a 50 kb and a 200 kb chromosome seen in L. (L.) mexicana and L. (L.) amazonensis, respectively, are stably maintained and linear. When used as a hybridisation probe, sc-2903 showed homology to large chromosomal DNA bands and to a multiplicity of genomic fragments ...
Tópico(s): Insect Resistance and Genetics
1993 - Elsevier BV | Acta Tropica