Y. Poorna Chandra Rao, Sugasini Dhavamani, Sridevi Dasarathi, Kalipada Pahan, Papasani V. Subbaiah,
... O. Lopes P.A. Ramos C. Miguueis S. Alfaia C.M. Pinto R.M.A. Rolo E. ... O. Lopes P.A. Ramos C. Miguueis S. Alfaia C.M. Pinto R.M.A. Rolo E. ...
Tópico(s): Adipose Tissue and Metabolism
2018 - Elsevier BV | Journal of Lipid Research
António J. Alfaia, Cristina M. Alfaia, Luís Patarata, Maria José Fernandes, Maria H. Fernandes, Miguel Elías, Maria H.L. Ribeiro, Maria João Fraqueza,
The effect of high pressure processing (HPP), at different combinations of pressure and time, on dry fermented sausages (DFS) was evaluated by chemical, microbiological and sensory analyses. Lipid composition and stability were also assessed. HPP (> 400 MPa and longer than 154 s) produced a reduction in spoilage microbiota, without negative effect on fermentative microbiota, that will be able to continue their role. Total fatty acids and lipid stability were not affected. Only a small effect on fatty ...
Tópico(s): Microbial Inactivation Methods
2015 - Elsevier BV | Innovative Food Science & Emerging Technologies
Cristina M. Alfaia, Paulo J.L.C. Ribeiro, Maria João Trigo, António J. Alfaia, Matilde Castro, C.M.G.A. Fontes, R.J.B. Bessa, José A. M. Prates,
The effect of gamma radiation processing on the lipid content, fatty acid composition and conjugated linoleic acid (CLA) profile in frozen lamb meat was investigated. Samples of longissimus thoracis muscle from lambs fed lucerne basal diets either unsupplemented or supplemented with polyunsaturated vegetable oils were irradiated (7kGy) and analysed. CLA contents in lamb meat did not affect (P>0.05) the levels of lipid oxidation induced by the irradiation. No significant differences (P>0.05) were observed ...
Tópico(s): Animal Nutrition and Physiology
2007 - Elsevier BV | Meat Science

Raquel Greice de Souza Marotta Alfaia, Ronei de Almeida, Kleby Soares do Nascimento, Juacyara Carbonelli Campos,
The present study investigates the effects of landfill leachate pretreatment by coagulation-flocculation (C/F) on the performance of nanofiltration (NF) and reverse osmosis (RO) membranes by comparing permeate flux, fouling resistance, and leachate quality. Pretreated leachates were produced by applying the C/F with ferric chloride (FeCl3) and alum sulfate (Al2(SO4)3), named P1 and P2 leachates. Raw leachate (RL), P1, and P2 samples were treated using five different polymeric membranes termed NP030, NP010, ...
Tópico(s): Environmental remediation with nanomaterials
2023 - Elsevier BV | Process Safety and Environmental Protection

Anderson Souto, Rodrigo Alfaia, Evelin Helena Silva Cardoso, Jasmine Araújo, Carlos Renato Lisboa Francês,
The use of unmanned aerial vehicles (UAVS) has been suggested as a potential communications alternative due to their fast implantation, which makes this resource an ideal solution to provide support in scenarios such as natural disasters or intentional attacks that may cause partial or complete disruption of telecommunications services. However, one limitation of this solution is energy autonomy, which affects mission life. With this in mind, our group has developed a new method based on reinforcement ...
Tópico(s): Smart Parking Systems Research
2023 - Multidisciplinary Digital Publishing Institute | Drones
Mónica M. Costa, Cristina M. Alfaia, Paula A. Lopes, José M. Pestana, José A. M. Prates,
Grape by-products are exceptional options for replacement of conventional and unsustainable feed sources, since large amounts are generated every year from the winery industry. However, the majority is wasted with severe environmental and economic consequences. The present review aimed to evaluate the effects of grape by-products on pig and poultry growth performance. The most recent literature was reviewed using ScienceDirect and PubMed databases and the results of a total of 16 and 38 papers for ...
Tópico(s): Horticultural and Viticultural Research
2022 - Multidisciplinary Digital Publishing Institute | Animals
David M. Ribeiro, Cristina M. Alfaia, José M. Pestana, Daniela F. P. Carvalho, Mónica M. Costa, Cátia F. Martins, J.P.C. Lemos, Miguel P. Mourato, Sandra Gueifão, Inês Delgado, Patrícia de Oliveira Carvalho, Diogo Coelho, Inês Coelho, João M. Freire, André M. Almeida, José A. M. Prates,
Laminaria digitata is a novel feedstuff that can be used in pig diets to replace conventional feedstuffs. However, its resilient cell wall can prevent the monogastric digestive system from accessing intracellular nutrients. Carbohydrate-active enzyme (CAZyme) supplementation is a putative solution for this problem, degrading the cell wall during digestion. The objective of this work was to evaluate the effect of 10% L. digitata feed inclusion and CAZyme supplementation on the meat quality and nutritional ...
Tópico(s): Animal Nutrition and Physiology
2022 - Multidisciplinary Digital Publishing Institute | Foods
Cátia F. Martins, Paolo Trevisi, Diogo Coelho, Federico Correa, David M. Ribeiro, Cristina M. Alfaia, Mário Pinho, José M. Pestana, Miguel P. Mourato, André M. Almeida, C.M.G.A. Fontes, J.P.B. Freire, José A. M. Prates,
Abstract The purpose of this study was to evaluate the impact of Chlorella vulgaris (5% in the diet), supplemented or not with two exogenous carbohydrase mixtures on piglets’ performance, nutrient digestibility and gut morphology, fermentation products and microbiota. Forty-four male piglets weaned at 28 days of age, with 11.2 ± 0.46 kg of live weight, were used and assigned to 1 of 4 dietary treatments: cereal and soybean meal based-diet (control, n = 11), control diet with 5% of C. vulgaris (CH, n = 10), ...
Tópico(s): Agriculture Sustainability and Environmental Impact
2022 - Nature Portfolio | Scientific Reports

Carlos A. Prete, Lewis Buss, Renata Buccheri, Claudia M. M. Abrahim, Tassila Salomon, Myuki Alfaia Esashika Crispim, Márcio K. Oikawa, Eduard Grebe, Allyson Guimarães Costa, Nelson Abrahim Fraiji, Maria do P. S. S. Carvalho, Charles Whittaker, Neâl Alexander, Nuno R. Faria, Christopher Dye, Vítor H. Nascimento, Michael P. Busch, Éster Cerdeira Sabino,
Abstract Background The city of Manaus, north Brazil, was stricken by a second epidemic wave of SARS-CoV-2 despite high seroprevalence estimates, coinciding with the emergence of the Gamma (P.1) variant. Reinfections were postulated as a partial explanation for the second surge. However, accurate calculation of reinfection rates is difficult when stringent criteria as two time-separated RT-PCR tests and/or genome sequencing are required. To estimate the proportion of reinfections caused by Gamma ...
Tópico(s): COVID-19 Clinical Research Studies
2022 - BioMed Central | BMC Infectious Diseases

Carlos A. Prete, Lewis Buss, Charles Whittaker, Tassila Salomon, Márcio K. Oikawa, Rafael HM Pereira, Isabel C. G. Moura, Lucas Delerino, Manoel Barral‐Netto, Natália Machado Tavares, Rafael Freitas de Oliveira França, Viviane Boaventura, Fábio Miyajima, Alfredo Mendrone, Cesar de Almeida‐Neto, Nanci A. Salles, Suzete C. Ferreira, Karine Amorim Fladzinski, Luana M. de Souza, Luciane K. Schier, Patricia M. Inoue, Lilyane Amorim Xábregas, Myuki Alfaia Esashika Crispim, Nelson Abrahim Fraiji, Fernando LV Araujo, Luciana M. B. Carlos, Veridiana Pessoa, Maísa Aparecida Ribeiro, Rosenvaldo E. de Souza, Sônia MN da Silva, Anna F. Cavalcante, Maria I. B. Valença, Maria V. da Silva, Esther Lopes, Luiz Amorim Filho, Sheila de Oliveira Garcia Mateos, Gabrielle Tomaz Nunes, Alexander Leonardo Silva-Junior, Michael P. Busch, Márcia C. Castro, Christopher Dye, Oliver Ratmann, Nuno R. Faria, Vítor H. Nascimento, Éster Cerdeira Sabino,
Background: The COVID-19 situation in Brazil is complex due to large differences in the shape and size of regional epidemics. Understanding these patterns is crucial to understand future outbreaks of SARS-CoV-2 or other respiratory pathogens in the country. Methods: We tested 97,950 blood donation samples for IgG antibodies from March 2020 to March 2021 in 8 of Brazil’s most populous cities. Residential postal codes were used to obtain representative samples. Weekly age- and sex-specific seroprevalence ...
Tópico(s): COVID-19 epidemiological studies
2022 - eLife Sciences Publications Ltd | eLife
Cristina M. Alfaia, Mónica M. Costa, Paula A. Lopes, José M. Pestana, José A. M. Prates,
Grape by-products could be used in monogastric animals' nutrition to reduce feeding costs with conventional crops (e.g., maize and soybean meal) and to improve meat quality. The main grape by-products with the largest expression worldwide, particularly in the Mediterranean region, are grape pomace, grape seed, grape seed oil and grape skins. These by-products are rich sources of bioactive polyphenols, dietary fiber and polyunsaturated fatty acids (PUFA), more specifically, the beneficial
Tópico(s): Nuts composition and effects
2022 - Multidisciplinary Digital Publishing Institute | Foods
Mónica M. Costa, Luís Pio, Pedro Bule, Vânia Cardoso, Marlene Duarte, Cristina M. Alfaia, Diogo Coelho, Joana L. A. Brás, C.M.G.A. Fontes, José A. M. Prates,
Green macroalgae, e.g., Ulva lactuca, are valuable bioactive sources of nutrients; but algae recalcitrant cell walls, composed of a complex cross-linked matrix of polysaccharides, can compromise their utilization as feedstuffs for monogastric animals. This study aimed to evaluate the ability of pre-selected Carbohydrate-Active enZymes (CAZymes) and sulfatases to degrade U. lactuca cell walls and release nutritive compounds. A databank of 199 recombinant CAZymes and sulfatases was tested in vitro ...
Tópico(s): Echinoderm biology and ecology
2022 - KeAi | Animal nutrition

William Marciel de Souza, Mariene R. Amorim, Renata Sesti‐Costa, Laís D. Coimbra, Natália S. Brunetti, Daniel A. Toledo-Teixeira, Gabriela Fabiano de Souza, Stéfanie Primon Muraro, Pierina Lorencini Parise, Priscilla P. Barbosa, Karina Bispo-dos-Santos, Luciana S. Mofatto, Camila L. Simeoni, Ingra Morales Claro, Adriana S S Duarte, Thaís M. Coletti, Audrey Basso Zangirolami, Carolina Costa-Lima, Arilson Bernardo S. P. Gomes, Lucas I Buscaratti, Flavia Cristina da Silva Sales, Vitor Antonio da Costa, Lucas Augusto Moysés Franco, Darlan S. Candido, Oliver G. Pybus, Jaqueline Góes de Jesus, Camila Alves Maia da Silva, Mariana Severo Ramundo, Giulia Magalhães Ferreira, Mariana C. Pinho, L. M. de S. e Souza, E Rocha, Pâmela dos Santos Andrade, Myuki Alfaia Esashika Crispim, Grazielle Celeste Maktura, Erika R. Manuli, Magnun Nueldo Nunes Santos, Cecília Cunha Camilo, Rodrigo Nogueira Angerami, Maria Luíza Moretti, Fernando Rosado Spilki, Clarice Weis Arns, Marcelo Addas‐Carvalho, Bruno Deltreggia Benites, Marco Aurélio Ramirez Vinolo, Marcelo A. Mori, Nelson Gaburo, Christopher Dye, Henrique Marques‐Souza, Rafael Elias Marques, Alessandro Farias, Michael Diamond, Nuno R. Faria, Éster Cerdeira Sabino, Fabiana Granja, José Luiz Proença-Módena,
Mutations accrued by SARS-CoV-2 lineage P.1-first detected in Brazil in early January, 2021-include amino acid changes in the receptor-binding domain of the viral spike protein that also are reported in other variants of concern, including B.1.1.7 and B.1.351. We aimed to investigate whether isolates of wild-type P.1 lineage SARS-CoV-2 can escape from neutralising antibodies generated by a polyclonal immune response.We did an immunological study to assess the neutralising effects of antibodies on ...
Tópico(s): COVID-19 Clinical Research Studies
2021 - Elsevier BV | The Lancet Microbe
Cátia F. Martins, David M. Ribeiro, Mónica M. Costa, Diogo Coelho, Cristina M. Alfaia, M.M. Lordelo, André M. Almeida, J.P.B. Freire, José A. M. Prates,
Cereal grains and soybean meal are the main feedstuffs used in swine and poultry feeding, two of the most consumed meats and of key relevance to food security worldwide. Such crops are grown mostly in North and South America and transported over large distances creating sustainability concerns and, furthermore, are in direct competition with human nutrition. Alternatives to these ingredients are, thus, a pressing need to ensure the sustainability of swine and poultry production. Microalgae seem ...
Tópico(s): Animal Nutrition and Physiology
2021 - Multidisciplinary Digital Publishing Institute | Foods

Nuno R. Faria, Thomas A. Mellan, Charles Whittaker, Ingra Morales Claro, Darlan da Silva Cândido, Swapnil Mishra, Myuki Alfaia Esashika Crispim, Flavia Cristina da Silva Sales, Iwona Hawryluk, John T. McCrone, Ruben J. G. Hulswit, Lucas Augusto Moysés Franco, Mariana Severo Ramundo, Jaqueline Góes de Jesus, Pâmela dos Santos Andrade, Thaís M. Coletti, Giulia Magalhães Ferreira, Camila Alves Maia da Silva, Erika R. Manuli, Rafael H. M. Pereira, Pedro S. Peixoto, Moritz U. G. Kraemer, Nelson Gaburo, Cecilia da C. Camilo, Henrique Hoeltgebaum, William Marciel de Souza, E Rocha, L. M. de S. e Souza, Mariana C. Pinho, Leonardo José Tadeu de Araújo, Frederico Scott Varella Malta, Aline Brito de Lima, Joice do Prado Silva, Danielle Alves Gomes Zauli, Alessandro Clayton de Souza Ferreira, Ricardo P. Schnekenberg, Daniel J. Laydon, Patrick Walker, Hannah M. Schlüter, Ana L. P. dos Santos, Maria S. Vidal, Valentina S. Del, Rosinaldo M. F. Filho, Helem M. dos Santos, Renato Santana Aguiar, José Luiz Proença-Módena, Bruce Nelson, James A. Hay, Mélodie Monod, Xenia Miscouridou, Helen Coupland, Raphael Sonabend, Michaela Vollmer, Axel Gandy, Carlos A. Prete, Vítor H. Nascimento, Marc A. Suchard, Thomas A. Bowden, Sergei L. Kosakovsky Pond, Chieh‐Hsi Wu, Oliver Ratmann, Neil M. Ferguson, Christopher Dye, Nicholas J. Loman, Philippe Lemey, Andrew Rambaut, Nelson Abrahim Fraiji, Maria do P. S. S. Carvalho, Oliver G. Pybus, Seth Flaxman, Samir Bhatt, Éster Cerdeira Sabino,
Cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in Manaus, Brazil, resurged in late 2020 despite previously high levels of infection. Genome sequencing of viruses sampled in Manaus between November 2020 and January 2021 revealed the emergence and circulation of a novel SARS-CoV-2 variant of concern. Lineage P.1 acquired 17 mutations, including a trio in the spike protein (K417T, E484K, and N501Y) associated with increased binding to the human ACE2 (angiotensin-converting ...
Tópico(s): COVID-19 Clinical Research Studies
2021 - American Association for the Advancement of Science | Science

Lewis Buss, Carlos A. Prete, Claudia M. M. Abrahim, Alfredo Mendrone, Tassila Salomon, Cesar de Almeida‐Neto, Rafael Freitas de Oliveira França, Maria Carolina T. D. Belotti, Maria P. S. S. Carvalho, Allyson Guimarães Costa, Myuki Alfaia Esashika Crispim, Suzete C. Ferreira, Nelson Abrahim Fraiji, Susie Gurzenda, Charles Whittaker, Leonardo Tadashi Kamaura, Pedro Losco Takecian, Pedro S. Peixoto, Márcio K. Oikawa, Anna S. Nishiya, Vanderson Rocha, Nanci A. Salles, Andreza Aruska de Souza Santos, Martirene A. da Silva, Brian Custer, Kris V. Parag, Manoel Barral‐Netto, Moritz U. G. Kraemer, Rafael H. M. Pereira, Oliver G. Pybus, Michael P. Busch, Márcia C. Castro, Christopher Dye, Vítor H. Nascimento, Nuno R. Faria, Éster Cerdeira Sabino,
Attack rate in Manaus Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) incidence peaked in Manaus, Brazil, in May 2020 with a devastating toll on the city's inhabitants, leaving its health services shattered and cemeteries overwhelmed. Buss et al. collected data from blood donors from Manaus and São Paulo, noted when transmission began to fall, and estimated the final attack rates in October 2020 (see the Perspective by Sridhar and Gurdasani). Heterogeneities in immune protection, population ...
Tópico(s): COVID-19 and Mental Health
2021 - American Association for the Advancement of Science | Science

Chantal B. F. Vogels, Mallery I. Breban, Isabel M. Ott, Tara Alpert, Mary E. Petrone, Anne E. Watkins, Chaney C. Kalinich, Rebecca Earnest, Jessica E. Rothman, Jaqueline Góes de Jesus, Ingra Morales Claro, Giulia Magalhães Ferreira, Myuki Alfaia Esashika Crispim, Lavanya Singh, Houriiyah Tegally, Ugochukwu J. Anyaneji, Emma B. Hodcroft, Christopher E. Mason, Gaurav Khullar, Jessica Metti, Joel T. Dudley, Matthew MacKay, Megan Nash, Jianhui Wang, Chen Liu, Pei Hui, Steven Murphy, Caleb Neal, Éva László, Marie L. Landry, Anthony Muyombwe, Randy Downing, Jafar Razeq, Túlio de Oliveira, Nuno R. Faria, Éster Cerdeira Sabino, Richard A. Neher, Joseph R Fauver, Nathan D. Grubaugh,
With the emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants that may increase transmissibility and/or cause escape from immune responses, there is an urgent need for the targeted surveillance of circulating lineages. It was found that the B.1.1.7 (also 501Y.V1) variant, first detected in the United Kingdom, could be serendipitously detected by the Thermo Fisher TaqPath COVID-19 PCR assay because a key deletion in these viruses, spike Δ69-70, would cause a "spike gene ...
Tópico(s): Animal Virus Infections Studies
2021 - Public Library of Science | PLoS Biology
David M. Ribeiro, Cátia F. Martins, Mónica M. Costa, Diogo Coelho, José M. Pestana, Cristina M. Alfaia, M.M. Lordelo, André M. Almeida, J.P.B. Freire, José A. M. Prates,
Seaweeds have caught the attention of the scientific community in recent years. Their production can mitigate the negative impact of anthropogenic activity and their use in animal nutrition reduces the dependency on conventional crops such as maize and soybean meal. In the context of monogastric animals, novel approaches have made it possible to optimise their use in feed, namely polysaccharide extraction, biomass fermentation, enzymatic processing, and feed supplementation with carbohydrate-active ...
Tópico(s): Aquaculture Nutrition and Growth
2021 - Multidisciplinary Digital Publishing Institute | Foods

Éster Cerdeira Sabino, Lewis Buss, Maria P. S. S. Carvalho, Carlos A. Prete, Myuki Alfaia Esashika Crispim, Nelson Abrahim Fraiji, Rafael H. M. Pereira, Kris V. Parag, Pedro S. Peixoto, Moritz U. G. Kraemer, Márcio K. Oikawa, Tassila Salomon, Zulma M. Cucunubá, Márcia C. Castro, Andreza Aruska de Souza Santos, Vítor H. Nascimento, Henrique dos Santos Pereira, Neil M. Ferguson, Oliver G. Pybus, Adam J. Kucharski, Michael P. Busch, Christopher Dye, Nuno R. Faria,
After initially containing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), many European and Asian countries had a resurgence of COVID-19 consistent with a large proportion of the population remaining susceptible to the virus after the first epidemic wave.1Lucy CO Verity R Watson OJ et al.Have deaths from COVID-19 in Europe plateaued due to herd immunity?.Lancet. 2020; 395: e110-e111Summary Full Text Full Text PDF PubMed Scopus (50) Google Scholar By contrast, in Manaus, Brazil, a study ...
Tópico(s): SARS-CoV-2 detection and testing
2021 - Elsevier BV | The Lancet

William Marciel de Souza, Mariene R. Amorim, Renata Sesti‐Costa, Laís D. Coimbra, Daniel A. Toledo-Teixeira, Pierina Lorencini Parise, Priscilla P. Barbosa, Karina Bispo-dos-Santos, Luciana S. Mofatto, Camila L. Simeoni, Natália S. Brunetti, Ingra Morales Claro, Adriana Silva Santos Duarte, Thaís M. Coletti, Audrey Basso Zangirolami, Carolina Costa-Lima, Arilson Bernardo S. P. Gomes, Lucas I Buscaratti, Flavia Cristina da Silva Sales, Vitor Antonio da Costa, Lucas Augusto Moysés Franco, Darlan S. Candido, Oliver G. Pybus, Jaqueline Góes de Jesus, Camila Alves Maia da Silva, Mariana Severo Ramundo, Giulia Magalhães Ferreira, Mariana C. Pinho, L. M. de S. e Souza, E Rocha, Pâmela dos Santos Andrade, Myuki Alfaia Esashika Crispim, Grazielle Celeste Maktura, Erika R. Manuli, Magnun Nueldo Nunes Santos, Cecília Cunha Camilo, Rodrigo Nogueira Angerami, Maria Luíza Moretti, Fernando Rosado Spilki, Clarice Weis Arns, Marcelo Addas‐Carvalho, Bruno Deltreggia Benites, Marcelo A. Mori, Nelson Gaburo, Christopher Dye, Chieh‐Hsi Wu, Henrique Marques‐Souza, Rafael Elias Marques, Alessandro Farias, Michael Diamond, Nuno R. Faria, Éster Cerdeira Sabino, Fabiana Granja, José Luiz Proença-Módena,
Background: A new SARS-CoV-2 lineage, named P.1 (20J/501Y.V3), has recently been detected in Brazil. Mutations accrued by the P.1 lineage include amino acid changes in the receptor-binding domain of the spike protein that also are reported in variants of concern in the United Kingdom (B.1.1.7) and South Africa (B.1.325).Methods: We isolated two P.1-containing specimens from nasopharyngeal and bronchoalveolar lavage samples of patients of Manaus, Brazil. We measured neutralization of the P.1 virus after ...
Tópico(s): Vaccine Coverage and Hesitancy
2021 - RELX Group (Netherlands) | SSRN Electronic Journal
Mónica M. Costa, Luís Pio, Pedro Bule, Vânia Cardoso, Cristina M. Alfaia, Diogo Coelho, Joana L. A. Brás, C.M.G.A. Fontes, José A. M. Prates,
Abstract In the present study, 199 pre-selected Carbohydrate-Active enZymes (CAZymes) and sulfatases were assessed, either alone or in combination, to evaluate their capacity to disrupt Laminaria digitata cell wall, with the consequent release of interesting nutritional compounds. A previously characterized individual alginate lyase, belonging to the family 7 of polysaccharide lyases (PL7) and produced by Saccharophagus degradans , was shown to be the most efficient in the in vitro degradation of L. ...
Tópico(s): Enzyme Production and Characterization
2021 - Nature Portfolio | Scientific Reports
Cátia F. Martins, José M. Pestana, Cristina M. Alfaia, Mónica M. Costa, David M. Ribeiro, Diogo Coelho, Paula A. Lopes, André M. Almeida, J.P.B. Freire, José A. M. Prates,
Chlorella vulgaris (CH) is usually considered a feed supplement in pig nutrition, and its use as an ingredient is poorly studied. Among many interesting characteristics, this microalga has high protein levels and can be a putative alternative for soybean meal. Our aim was to study the effect of a 5% CH incorporation in the diet, individually or combined with two carbohydrases, on meat quality traits and nutritional value. Forty-four post-weaned male piglets individually housed, with an initial live ...
Tópico(s): Meat and Animal Product Quality
2021 - Multidisciplinary Digital Publishing Institute | Foods

Raquel Scherer de Fraga, Victor Van Vaisberg, Luiz Cláudio Alfaia Mendes, Flair José Carrilho, Ono S,
Abstract Nucleos(t)ide analogues (NAs) are the main drug category used in chronic hepatitis B (CHB) treatment. Despite the fact that NAs have a favourable safety profile, undesired adverse events (AEs) may occur during the treatment of CHB. Given the eminent number of patients currently receiving NAs, even a small risk of any of these toxicities can represent a major medical issue. The main objective of this review was to analyse information available on AEs associated with the use of NAs in published ...
Tópico(s): HIV/AIDS drug development and treatment
2020 - Springer Science+Business Media | Journal of Gastroenterology
Diogo Coelho, José M. Pestana, J. Almeida, Cristina M. Alfaia, C.M.G.A. Fontes, Olga Moreira, José A. M. Prates,
The influence of a high inclusion level of Chlorella vulgaris, individually and supplemented with two carbohydrase mixtures, in finishing pig diets was assessed on zootechnical performance, carcass characteristics, pork quality traits and nutritional value of pork fat. Forty crossbred entire male pigs, sons of Large White × Landrace sows crossed with Pietrain boars, with an initial live weight of 59.1 ± 5.69 kg were used in this trial. Swines were randomly assigned to one of four dietary treatments ( ...
Tópico(s): Algal biology and biofuel production
2020 - Multidisciplinary Digital Publishing Institute | Animals

Marília Rosa Abtibol‐Bernardino, Lucíola de Fátima Albuquerque de Almeida Peixoto, Geruza Alfaia de Oliveira, Tatiane Freitas de Almeida, Gabriela Ribeiro Ivo Rodrigues, Rodrigo Haruo Otani, Beatriz Caroline Soares Chaves, Cristina de Souza Rodrigues, Anny Beatriz Costa Antony de Andrade, Elijane de Fátima Redivo, Salete Sara Alvarez Fernandes, Márcia da Costa Castilho, Silvana Gomes Benzecry, Camila Bôtto-Menezes, Flor Ernestina Martínez-Espinosa, Maria das Graças Costa Alecrim,
The Zika virus can induce a disruptive sequence in the fetal brain and is manifested mainly by microcephaly. Knowledge gaps still exist as to whether the virus can cause minor disorders that are perceived later on during the first years of life in children who are exposed but are asymptomatic at birth. In this case series, we describe the outcomes related to neurodevelopment through the neurological assessment of 26 non-microcephalic children who had intrauterine exposure to Zika virus. Children ...
Tópico(s): Virology and Viral Diseases
2020 - Multidisciplinary Digital Publishing Institute | Viruses
Cristina M. Alfaia, José M. Pestana, Marcoaurélio Almenara Rodrigues, Diogo Coelho, Manuel Aires, David M. Ribeiro, V.T. Major, Cátia F. Martins, Helena Santos, Paula A. Lopes, J.P.C. Lemos, C.M.G.A. Fontes, M.M. Lordelo, José A. M. Prates,
Herein, we investigated the effect of Chlorella vulgaris as ingredient (10% of incorporation) in broiler diets, supplemented or not with 2 formulations of Carbohydrate-Active enZymes (CAZymes; Rovabio Excel AP and a mixture of recombinant CAZymes, composed by an exo-β-glucosaminidase, an alginate lyase, a peptidoglycan N-acetylmuramic acid deacetylase and a lysozyme), on growth performance, meat quality, fatty acid composition, oxidative stability, and sensory traits. One hundred twenty 1-day-old ...
Tópico(s): Animal Nutrition and Physiology
2020 - Elsevier BV | Poultry Science
Cátia F. Martins, José Pestana Assunção, David M. Ribeiro, Marta Sofia Morgado dos S. Madeira, Cristina M. Alfaia, Paula A. Lopes, Diogo Coelho, J.P.C. Lemos, André M. Almeida, José A. M. Prates, J.P.B. Freire,
The effect of Spirulina (Arthrospira platensis), individually or in combination with two commercial carbohydrases, in piglet diets was assessed on growth performance, nutrient digestibility and meat quality traits. Forty post-weaned male piglets from Large White × Landrace sows crossed with Pietrain boars with an initial live weight of 12.0 ± 0.89 kg were used. Piglets were assigned to one of four dietary treatments (n = 10): cereal and soya bean meal base diet (control), base diet with 10% Spirulina (SP), ...
Tópico(s): Ruminant Nutrition and Digestive Physiology
2020 - Wiley | Journal of Animal Physiology and Animal Nutrition
José M. Pestana, B. Puerta, Helena Santos, Marta S. Madeira, Cristina M. Alfaia, Paula A. Lopes, Rui Pinto, J.P.C. Lemos, C.M.G.A. Fontes, M.M. Lordelo, José A. M. Prates,
This study assessed the effect of Spirulina (Arthrospira platensis), individually and in combination with exogenous enzymes, on growth performance, carcass traits, and meat quality of broiler chickens. One hundred and twenty Ross 308 male chickens were allocated into 40 battery brooders, with 3 birds per cage, and fed ad libitum a corn-based diet during the first 21 D of the trial. The experimental period lasted from day 21 to 35, during which birds were fed 4 different diets: a corn-soybean basal ...
Tópico(s): Antioxidant Activity and Oxidative Stress
2020 - Elsevier BV | Poultry Science
Werica Larissa Farias de Vasconcelos, Diego de Macedo Rodrigues, Rafael Oliveira Carvalho Silva, Sônia Sena Alfaia,
Given the influence of edaphic macrofauna in the physical, chemical, and biological processes that sustain the organic matter cycle in the soil of tropical ecosystems, this study aimed to evaluate the effect of the sequence: Secondary Forest – Pasture – Eucalyptus monoculture on the macrofauna structure in Southeast of Pará State, Brazil. In each land use system, two 350 m transect were taken. The data was collected in 8 sampling sites, which were 50 m apart in each transect [...]
Tópico(s): Isotope Analysis in Ecology
2020 - Sociedade Brasileira de Ciência do Solo | Revista Brasileira de Ciência do Solo

Mauro Ângelo Oliveira de Alfaia, Ricardo Oliveira, Thiago Soares Lima, Fábio Edson Mariani, Luíz Carlos Casteletti, Noé Cheung, Amauri Garcia,
Aluminum alloys with soft elements are quite promising in the automotive industry for manufacturing machine elements subjected to relative motion. The combination of some elements makes it possible to highlight some properties, such as the wear resistance. It is also particularly important to understand the role of the microstructural phases’ representative scale in this property. In this research, the joint influence of Bi and Pb on Al-3.2 wt.%Bi-(1; 3) wt.%Pb alloys is analyzed. The alloys were ...
Tópico(s): Microstructure and mechanical properties
2020 - Elsevier BV | Materials Today Communications