Francisco Estay, Roberto Neira, Nelson F. Díaz, Luis Valladares, Alfredo Torres,
... ChileSearch for more papers by this authorAlfredo Torres, Alfredo Torres Fish Culture Center, IFOP, Coyhaique, ChileSearch for more ... ChileSearch for more papers by this authorAlfredo Torres, Alfredo Torres Fish Culture Center, IFOP, Coyhaique, ChileSearch for more ...
Tópico(s): Fish Ecology and Management Studies
1998 - Wiley | Journal of Experimental Zoology
Laura A. Muruato, Alfredo G. Torres,
... development of an effective vaccine?Laura A Muruato & Alfredo G Torres Laura A Muruato Department of Microbiology & Immunology, Institute ... of Texas Medical Branch, Galveston, TX 77555, USA & Alfredo G Torres*Author for correspondence: Tel.: +1 409 747 0189; ...
Tópico(s): Brucella: diagnosis, epidemiology, treatment
2016 - Future Medicine | Future Microbiology
Juan Morán, Gustavo Ramírez-Martínez, Luis Jiménez-Álvarez, Alfredo Torres Cruz, Santiago Pérez-Patrigeón, Alfredo Hidalgo‐Miranda, Lorena Orozco, Angélica Martínez, Luis Padilla-Noriega, Federico Ávila‐Moreno, Carlos Cabello-Gutiérrez, Julio Granados, Blanca Ortíz-Quintero, Alejandra Ramírez‐Venegas, Guillermo M. Ruiz‐Palacios, Albert Zlotnik, Enrique Merino, Joaquı́n Zúñiga,
Overproduction of pro-inflammatory cytokines and chemokines is frequently associated with severe clinical manifestations in patients infected with influenza A/H1N1 virus. Micro-RNAs (miRNAs) are highly conserved small non-coding RNA molecules that post-transcriptionally regulate gene expression and are potential biomarkers and therapeutic targets in different inflammatory conditions.We studied the circulating and miRNA profiles in critically ill A/H1N1 patients, A/H1N1 patients with milder disease, ...
Tópico(s): Cancer-related molecular mechanisms research
2015 - Elsevier BV | Experimental and Molecular Pathology
Produção Nacional
Revisado por pares
Joaquı́n Zúñiga, Ivette Buendia‐Roldán, Yang Zhao, Luís Jiménez, Diana Torres‐García, Javier Romo, Gustavo Ramírez, Alfredo Torres Cruz, Gilberto Vargas‐Alarcón, Chau‐Chyun Sheu, Feng Chen, Su Li, AM Tager, Annie Pardo, Moisés Selman, David C. Christiani,
The A/H1N1 influenza strain isolated in Mexico in 2009 caused severe pulmonary illness in a small number of exposed individuals. Our objective was to determine the influence of genetic factors on their susceptibility. We carried out a case–control association study genotyping 91 patients with confirmed severe pneumonia from A/H1N1 infection and 98 exposed but asymptomatic household contacts, using the HumanCVD BeadChip (Illumina, San Diego, CA, USA). Four risk single-nucleotide polymorphisms were ...
Tópico(s): interferon and immune responses
2011 - European Respiratory Society | European Respiratory Journal
Joaquı́n Zúñiga, Martha Torres, Javier Romo, Diana Torres‐García, Luís Carlos Villamil Jiménez, Gustavo Ramírez, Alfredo Torres Cruz, Enrique Espinosa, María Teresa Herrera, Ivette Buendia‐Roldán, Alejandra Ramírez‐Venegas, Yolanda González, Karen Bobadilla, Fernando Hernández, Jorge A. García, Francisco Quiñones-Falconi, Eduardo Sada, María Eugenia Manjarrez, Carlos Cabello-Gutiérrez, Simón Kawa, Albert Zlotnik, Annie Pardo, Moisés Selman,
The immune mechanisms underlying the pathogenesis of severe pneumonia associated with the A/H1N1 virus are not well known. The objective of this study was to determine whether severe A/H1N1-associated pneumonia can be explained by the emergence of particular T-cell subsets and the cytokines/chemokines they produced, as well as distinct responses to infection. T-cell subset distribution and cytokine/chemokine levels in peripheral blood and bronchoalveolar lavage (BAL) were determined in patients with ...
Tópico(s): Immune Response and Inflammation
2011 - Informa | Autoimmunity
Paola L. García‐Medel, Noe Baruch‐Torres, Antolín Peralta-Castro, Carlos H. Trasviña‐Arenas, Alfredo Torres‐Larios, Luis G. Brieba,
Double-stranded breaks (DSBs) in plant organelles are repaired via genomic rearrangements characterized by microhomologous repeats. These microhomologous signatures predict the existence of an unidentified enzymatic machinery capable of repairing of DSBs via microhomology-mediated end-joining (MMEJ) in plant organelles. Here, we show that organellar DNA polymerases from Arabidopsis thaliana (AtPolIA and AtPolIB) perform MMEJ using microhomologous sequences as short as six nucleotides. AtPolIs execute ...
Tópico(s): DNA Repair Mechanisms
2019 - Oxford University Press | Nucleic Acids Research
Itzhel García‐Torres, Nallely Cabrera, Alfredo Torres‐Larios, Mónica Rodríguez-Bolaños, Selma Díaz-Mazariegos, Armando Gómez‐Puyou, Ruy Pérez‐Montfort,
For a better comprehension of the structure-function relationship in proteins it is necessary to identify the amino acids that are relevant for measurable protein functions. Because of the numerous contacts that amino acids establish within proteins and the cooperative nature of their interactions, it is difficult to achieve this goal. Thus, the study of protein-ligand interactions is usually focused on local environmental structural differences. Here, using a pair of triosephosphate isomerase enzymes ...
Tópico(s): Biochemical and Molecular Research
2011 - Public Library of Science | PLoS ONE
Joel Salazar‐Flores, Luis A. Torres-Reyes, G. Martínez-Cortés, Rodrigo Rubí‐Castellanos, Martha Sosa‐Macías, José Francisco Muñoz-Valle, César González‐González, Angélica V. Angulo Ramírez, Raquel Román, José L. Méndez, Andrés Barrera, Alfredo Torres, R.A. Medina López, Héctor Rangel‐Villalobos,
Background: The distribution of polymorphisms in the CYP2D6 and CYP2C19 genes allows inferring the potential risk for specific adverse drug reactions and lack of therapeutic effects in humans. This variability shows differences among human populations. The aim of this study was to analyze single-nucleotide polymorphisms related to a poor metabolizer (PM) phenotype in nonpreviously studied Amerindian groups and Mestizos (general admixed population) from Mexico. Methods: We detected by SNaPshot® different ...
Tópico(s): Inflammatory mediators and NSAID effects
2012 - Mary Ann Liebert, Inc. | Genetic Testing and Molecular Biomarkers
Jonathan G. Rodríguez Plaza, Amanda Villalón Rojas, Sur Herrera Paredes, Georgina Garza‐Ramos, Alfredo Torres‐Larios, Carlos Amero, Gabriela Zarraga Granados, Manuel Gutiérrez‐Aguilar, María Teresa Lara Ortiz, Carlos Polanco, Salvador Uribe‐Carvajal, Roberto Coria, Antonio Peña Díaz, Dale E. Bredesen, Susana Castro‐Obregón, Gabriel del Rio,
Hunter-killer peptides combine two activities in a single polypeptide that work in an independent fashion like many other multi-functional, multi-domain proteins. We hypothesize that emergent functions may result from the combination of two or more activities in a single protein domain and that could be a mechanism selected in nature to form moonlighting proteins. We designed moonlighting peptides using the two mechanisms proposed to be involved in the evolution of such molecules (i.e., to mutate ...
Tópico(s): Fungal and yeast genetics research
2012 - Public Library of Science | PLoS ONE
Alfredo Torres‐Larios, Kerren K. Swinger, Andrey S. Krasilnikov, Tao Pan, Alfonso Mondragón,
Tópico(s): Genomics and Phylogenetic Studies
2005 - Nature Portfolio | Nature
Anne‐Catherine Dock‐Brégeon, B. Rees, Alfredo Torres‐Larios, Gilbert Bey, J. Caillet, Dino Moras,
The fidelity of aminoacylation of tRNAThr by the threonyl-tRNA synthetase (ThrRS) requires the discrimination of the cognate substrate threonine from the noncognate serine. Misacylation by serine is corrected in a proofreading or editing step. An editing site has been located 39 Å away from the aminoacylation site. We report the crystal structures of this editing domain in its apo form and in complex with the serine product, and with two nonhydrolyzable analogs of potential substrates: the terminal ...
Tópico(s): RNA Research and Splicing
2004 - Elsevier BV | Molecular Cell
Alfredo Torres‐Larios, Anne‐Catherine Dock‐Brégeon, Pascale Romby, B. Rees, Rajan Sankaranarayanan, J. Caillet, Mathias Springer, Chantal Ehresmann, Bernard Ehresmann, Dino Moras,
Tópico(s): Bacterial Genetics and Biotechnology
2002 - Nature Portfolio | Nature Structural & Molecular Biology
J. Caillet, Teresa Nogueira, Benoı̂t Masquida, Flore Winter, M. Graffe, Anne‐Catherine Dock‐Brégeon, Alfredo Torres‐Larios, Rajan Sankaranarayanan, Éric Westhof, Bernard Ehresmann, Chantal Ehresmann, Pascale Romby, Mathias Springer,
Summary In addition to its role in tRNA aminoacylation, Escherichia coli threonyl‐tRNA synthetase is a regulatory protein which binds a site, called the operator, located in the leader of its own mRNA and inhibits translational initiation by competing with ribosome binding. This work shows that the two essential steps of regulation, operator recognition and inhibition of ribosome binding, are performed by different domains of the protein. The catalytic and the C‐terminal domain of the protein are ...
Tópico(s): Bacterial Genetics and Biotechnology
2003 - Wiley | Molecular Microbiology
Alfredo Torres‐Larios, Rajan Sankaranarayanan, B. Rees, Anne‐Catherine Dock‐Brégeon, Dino Moras,
The crystal structures of threonyl-tRNA synthetase (ThrRS) from Staphylococcus aureus, with ATP and an analogue of threonyl adenylate, are described. Together with the previously determined structures of Escherichia coli ThrRS with different substrates, they allow a comprehensive analysis of the effect of binding of all the substrates: threonine, ATP and tRNA. The tRNA, by inserting its acceptor arm between the N-terminal domain and the catalytic domain, causes a large rotation of the former. Within ...
Tópico(s): Bacterial Genetics and Biotechnology
2003 - Elsevier BV | Journal of Molecular Biology
Claudia Rodríguez-Almazán, Rodrigo Arreola, David Rodríguez‐Larrea, Beatriz Aguirre‐López, Marietta Tuena de Gómez‐Puyou, Ruy Pérez‐Montfort, Miguel Costas, Armando Gómez‐Puyou, Alfredo Torres‐Larios,
Human triosephosphate isomerase deficiency is a rare autosomal disease that causes premature death of homozygous individuals. The most frequent mutation that leads to this illness is in position 104, which involves a conservative change of a Glu for Asp. Despite the extensive work that has been carried out on the E104D mutant enzyme in hemolysates and whole cells, the molecular basis of this disease is poorly understood. Here, we show that the purified, recombinant mutant enzyme E104D, while exhibiting ...
Tópico(s): Biomedical Research and Pathophysiology
2008 - Elsevier BV | Journal of Biological Chemistry
Nicholas J. Reiter, Amy Osterman, Alfredo Torres‐Larios, Kerren K. Swinger, Tao Pan, Alfonso Mondragón,
Ribonuclease (RNase) P is the universal ribozyme responsible for 5′-end tRNA processing. We report the crystal structure of the Thermotoga maritima RNase P holoenzyme in complex with tRNAPhe. The 154 kDa complex consists of a large catalytic RNA (P RNA), a small protein cofactor and a mature tRNA. The structure shows that RNA–RNA recognition occurs through shape complementarity, specific intermolecular contacts and base-pairing interactions. Soaks with a pre-tRNA 5′ leader sequence with and without ...
Tópico(s): Bacterial Genetics and Biotechnology
2010 - Nature Portfolio | Nature
Faviola Tavares-Carreón, Yolanda Camacho‐Villasana, Angélica Zamudio-Ochoa, Miguel Shingú-Vázquez, Alfredo Torres‐Larios, Xóchitl Pérez-Martínez,
Pet309 is a protein essential for respiratory growth. It is involved in translation of the yeast mitochondrial COX1 gene, which encodes subunit I of the cytochrome c oxidase. Pet309 is also involved in stabilization of the COX1 mRNA. Mutations in a similar human protein, Lrp130, are associated with Leigh syndrome, where cytochrome c oxidase activity is affected. The sequence of Pet309 reveals the presence of at least seven pentatricopeptide repeats (PPRs) located in tandem in the central portion ...
Tópico(s): Metalloenzymes and iron-sulfur proteins
2007 - Elsevier BV | Journal of Biological Chemistry
Juan C. Canul-Tec, Lidia Riaño‐Umbarila, E. Rudiño-Piñera, Baltazar Becerril, Lourival D. Possani, Alfredo Torres‐Larios,
It has previously been reported that several single-chain antibody fragments of human origin (scFv) neutralize the effects of two different scorpion venoms through interactions with the primary toxins of Centruroides noxius Hoffmann (Cn2) and Centruroides suffusus suffusus (Css2). Here we present the crystal structure of the complex formed between one scFv (9004G) and the Cn2 toxin, determined in two crystal forms at 2.5 and 1.9 Å resolution. A 15-residue span of the toxin is recognized by the antibody ...
Tópico(s): Ion channel regulation and function
2011 - Elsevier BV | Journal of Biological Chemistry
Ángel G. Díaz‐Sánchez, Lilian González‐Segura, E. Rudiño-Piñera, Alfonso Lira‐Rocha, Alfredo Torres‐Larios, Rosario A. Muñoz‐Clares,
PaBADH (Pseudomonas aeruginosa betaine aldehyde dehydrogenase) catalyses the irreversible NAD(P)+-dependent oxidation of betaine aldehyde to its corresponding acid, the osmoprotector glycine betaine. This reaction is involved in the catabolism of choline and in the response of this important pathogen to the osmotic and oxidative stresses prevalent in infection sites. The crystal structure of PaBADH in complex with NADPH showed a novel covalent adduct between the C2N of the pyridine ring and the sulfur ...
Tópico(s): Alcoholism and Thiamine Deficiency
2011 - Portland Press | Biochemical Journal
Alfredo Torres‐Larios, Kerren K. Swinger, Tao Pan, Alfonso Mondragón,
Ribonuclease P (RNase P) is one of only two known universal ribozymes and was one of the first ribozymes to be discovered. It is involved in RNA processing, in particular the 5' maturation of tRNA. Unlike most other natural ribozymes, it recognizes and cleaves its substrate in trans. RNase P is a ribonucleoprotein complex containing one RNA subunit and as few as one protein subunit. It has been shown that, in bacteria and in some archaea, the RNA subunit alone can support catalysis. The structure ...
Tópico(s): Bacteriophages and microbial interactions
2006 - Elsevier BV | Current Opinion in Structural Biology
José Alejandro Martínez‐Ibarra, Ricardo Alejandre‐Aguilar, Alfredo Torres-Morales, Josefina Cecilia Trujillo-García, Benjamín Nogueda‐Torres, Francisco Trujillo-Contreras,
Aspects related to hatching, life time, number of blood meals to molt, mortality, feeding time and postfeed defecation delay for each instar of Meccus phyllosomus, M. mazzottii, and M. bassolsae, life-cycle were evaluated and compared in two cohorts of each of those three species, fed on hens or rabbits. No significant (p > 0.05) differences were recorded among cohorts fed on hens respect to cohorts fed on rabbits in M. phyllosomus and M. mazzottii and the average time of hatching was 21.5 days for ...
Tópico(s): Insect-Plant Interactions and Control
2006 - Instituto Oswaldo Cruz, Ministério da Saúde | Memórias do Instituto Oswaldo Cruz
Aaron Hernandez‐Cid, Sergio Aguirre‐Sampieri, A. Diaz-Vilchis, Alfredo Torres‐Larios,
Abstract One of the hallmarks of life is the widespread use of certain essential ribozymes. The ubiquitous ribonuclease P (RNase P) and eukaryotic RNase MRP are essential complexes where a structured, noncoding RNA acts in catalysis. Recent discoveries have elucidated the three‐dimensional structure of the ancestral ribonucleoprotein complex, suggested the possibility of a protein‐only composition in organelles, and even noted the absence of RNase P in a non‐free‐living organism. With respect to ...
Tópico(s): Genomics and Phylogenetic Studies
2012 - Wiley | IUBMB Life
Alfredo Torres‐Larios, Georgina B. Gurrola, Fernando Z. Zamudio, Lourival D. Possani,
A new antimicrobial peptide, hadrurin, was isolated from the venom of the Mexican scorpion Hadrurus aztecus , by gel filtration on a Sephadex G‐50 column, followed by high performance liquid chromatography. It is a basic peptide composed of 41 amino‐acid residues with a molecular mass of 4436 Da, and contains no cysteines. A model of the three‐dimensional folding of hadrurin is compatible with that of an amphipatic molecule with two α‐helical segments. Hadrurin demonstrates antimicrobial activity ...
Tópico(s): Venomous Animal Envenomation and Studies
2000 - Wiley | European Journal of Biochemistry
Carlos Pérez-Guzmán, Alfredo Torres-Cruz, H Villarreal-Velarde, Mario H. Vargas,
Atypical radiologic images of pulmonary tuberculosis are common in elderly and in diabetic patients. To investigate the relationship of chest radiographic findings of tuberculosis to age in diabetic and nondiabetic patients, we compared the chest radiographic findings of 192 inpatients with pulmonary tuberculosis and diabetes with those of 130 patients with pulmonary tuberculosis alone. The proportion of patients with lower lung field lesions progressively increased with age (rS = 0.89, p < 0.01), whereas ...
Tópico(s): Infectious Diseases and Tuberculosis
2000 - American Thoracic Society | American Journal of Respiratory and Critical Care Medicine
J. Domingo Meza-Aguilar, Petra Fromme, Alfredo Torres‐Larios, Guillermo Mendoza‐Hernández, Ulises Hernández‐Chiñas, Roberto Arreguı́n-Espinosa, Carlos Alberto Eslava Campos, Raimund Fromme,
Autotransporters (ATs) represent a superfamily of proteins produced by a variety of pathogenic bacteria, which include the pathogenic groups of Escherichia coli (E. coli) associated with gastrointestinal and urinary tract infections. We present the first X-ray structure of the passenger domain from the Plasmid-encoded toxin (Pet) a 100 kDa protein at 2.3 Å resolution which is a cause of acute diarrhea in both developing and industrialized countries. Pet is a cytoskeleton-altering toxin that induces ...
Tópico(s): Viral gastroenteritis research and epidemiology
2014 - Elsevier BV | Biochemical and Biophysical Research Communications
De Ji, Alfredo Torres‐Larios, Guillermo Mendoza‐Hernández, Sergio Enríquez‐Flores, Adriana Castillo‐Villanueva, Sara T. Méndez, Itzhel García‐Torres, Angélica Torres‐Arroyo, Saúl Gómez‐Manzo, Jaime Marcial‐Quino, Jesús Oria‐Hernández, Gabriel López‐Velázquez, Horacio Reyes‐Vivas,
The deficiency of human triosephosphate isomerase (HsTIM) generates neurological alterations, cardiomyopathy and premature death. The mutation E104D is the most frequent cause of the disease. Although the wild type and mutant exhibit similar kinetic parameters, it has been shown that the E104D substitution induces perturbation of an interfacial water network that, in turn, reduces the association constant between subunits promoting enzyme inactivation. To gain further insight into the effects of ...
Tópico(s): Blood properties and coagulation
2013 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
Gloria Hernández‐Alcántara, Alfredo Torres‐Larios, Sergio Enríquez‐Flores, Itzhel García‐Torres, Adriana Castillo‐Villanueva, Sara T. Méndez, De Ji, Saúl Gómez‐Manzo, Angélica Torres‐Arroyo, Gabriel López‐Velázquez, Horacio Reyes‐Vivas, Jesús Oria‐Hernández,
Background We have previously proposed triosephosphate isomerase of Giardia lamblia (GlTIM) as a target for rational drug design against giardiasis, one of the most common parasitic infections in humans. Since the enzyme exists in the parasite and the host, selective inhibition is a major challenge because essential regions that could be considered molecular targets are highly conserved. Previous biochemical evidence showed that chemical modification of the non-conserved non-catalytic cysteine 222 ( ...
Tópico(s): Erythrocyte Function and Pathophysiology
2013 - Public Library of Science | PLoS ONE
De Ji, Alfredo Torres‐Larios, Sergio Enríquez‐Flores, Sara T. Méndez, Adriana Castillo‐Villanueva, Saúl Gómez‐Manzo, Gabriel López‐Velázquez, Jaime Marcial‐Quino, Angélica Torres‐Arroyo, Itzhel García‐Torres, Horacio Reyes‐Vivas, Jesús Oria‐Hernández,
Deamidation, the loss of the ammonium group of asparagine and glutamine to form aspartic and glutamic acid, is one of the most commonly occurring post-translational modifications in proteins. Since deamidation rates are encoded in the protein structure, it has been proposed that they can serve as molecular clocks for the timing of biological processes such as protein turnover, development and aging. Despite the importance of this process, there is a lack of detailed structural information explaining ...
Tópico(s): Enzyme Structure and Function
2015 - Public Library of Science | PLoS ONE
Beatriz Sepúlveda, Cristina Quispe, Mario J. Simirgiotis, Alfredo Torres-Benítez, Johanna Reyes-Ortíz, Carlos Areche, Olimpo García‐Beltrán,
Natural or synthetic coumarins showed gastroprotective and antiulcer activity in animal models. In this study, we have synthetized twenty coumarins using classic methods to evaluate their gastroprotective effects on the ethanol/HCl-induced gastric lesion model in mice at 20 mg/kg. Among the coumarins synthetized, compounds 6 and 10 showed the greatest gastroprotective activity being as active as lansoprazole at 20 mg/kg and reducing gastric lesions by 75 and 76%, respectively. Then, in a second ...
Tópico(s): Eicosanoids and Hypertension Pharmacology
2016 - Elsevier BV | Bioorganic & Medicinal Chemistry Letters
Israel Mares‐Mejía, Siseth Martínez‐Caballero, Claudia Angélica Garay-Canales, Patricia Cano‐Sánchez, Alfredo Torres‐Larios, Samuel Lara‐González, Enrique Ortega, Adela Rodríguez‐Romero,
Abstract Oligomerization of allergens plays an important role in IgE-mediated reactions, as effective crosslinking of IgE- FcεRI complexes on the cell membrane is dependent on the number of exposed B-cell epitopes in a single allergen molecule or on the occurrence of identical epitopes in a symmetrical arrangement. Few studies have attempted to experimentally demonstrate the connection between allergen dimerization and the ability to trigger allergic reactions. Here we studied plant allergenic profilins ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2016 - Nature Portfolio | Scientific Reports