Produção Nacional
Revisado por pares
Maria Fernanda Forni, Wilson Garcia-Neto, Alicia J. Kowaltowski, Guilherme Andrade Marson,
... this authorAlicia J Kowaltowski, Corresponding Author Alicia J Kowaltowski alicia@iq.usp.br orcid.org/0000-0002-3807- ... this authorAlicia J Kowaltowski, Corresponding Author Alicia J Kowaltowski alicia@iq.usp.br orcid.org/0000-0002-3807-2419 Correspondence: Alicia Kowaltowski, Departamento de Bioquímica, Instituto de Química, Universidade de ...
Tópico(s): Cancer and biochemical research
2017 - Wiley | Medical Education
... citing this article:Fernanda M. Cerqueira, Bruno Chausse, Alicia J. Kowaltowski Intermittent Fasting Effects on the Central Nervous System: ... 319-55387-0_29Fernanda M. Cerqueira, Bruno Chausse, Alicia J. Kowaltowski Intermittent Fasting Effects on the Central Nervous System: ...
Tópico(s): Dietary Effects on Health
1913 - University of Chicago Press | The American Naturalist
Produção Nacional
Revisado por pares
Heberty Tarso Facundo, Alicia J. Kowaltowski,
... Mitochondrial Permeability Transition" Heberty T.F. Facundo and Alicia J. Kowaltowski Heberty T.F. FacundoHeberty T.F. Facundo Department ... University of São Paulo, São Paulo, Brazil, and Alicia J. KowaltowskiAlicia J. Kowaltowski Department of Biochemistry, University of São Paulo, São ...
Tópico(s): Anesthesia and Neurotoxicity Research
2005 - Lippincott Williams & Wilkins | Circulation
Produção Nacional
Revisado por pares
Luciane C. Alberici, Helena C.F. Oliveira, Bruno A. Paim, Camila Campos Mantello, Amanda C. Augusto, Karina G. Zecchin, Sonia A. Gurgueira, Alicia J. Kowaltowski, Anı́bal E. Vercesi,
We have recently demonstrated that hypertriglyceridemic (HTG) mice present both elevated body metabolic rates and mild mitochondrial uncoupling in the liver owing to stimulated activity of the ATP-sensitive potassium channel (mitoK(ATP)). Because lipid excess normally leads to cell redox imbalance, we examined the hepatic oxidative status in this model. Cell redox imbalance was evidenced by increased total levels of carbonylated proteins, malondialdehydes, and GSSG/GSH ratios in HTG livers compared ...
Tópico(s): Adipose Tissue and Metabolism
2009 - Elsevier BV | Free Radical Biology and Medicine
Produção Nacional
Revisado por pares
Alicia J. Kowaltowski, Nadja C. de Souza‐Pinto, Roger F. Castilho, Anı́bal E. Vercesi,
Mitochondria are a quantitatively relevant source of reactive oxygen species (ROS) in the majority of cell types. Here we review the sources and metabolism of ROS in this organelle, including the conditions that regulate the production of these species, such as mild uncoupling, oxygen tension, respiratory inhibition, Ca2+ and K+ transport, and mitochondrial content and morphology. We discuss substrate-, tissue-, and organism-specific characteristics of mitochondrial oxidant generation. Several aspects ...
Tópico(s): Redox biology and oxidative stress
2009 - Elsevier BV | Free Radical Biology and Medicine
Produção Nacional
Revisado por pares
Julio César Carranza, Alicia J. Kowaltowski, Marco Aurélio G. Mendonça, Thays C. de Oliveira, Fernanda Ramos Gadelha, Bianca Zingales,
Tópico(s): Mitochondrial Function and Pathology
2009 - Springer Science+Business Media | Journal of Bioenergetics and Biomembranes
Produção Nacional
Revisado por pares
Erich Birelli Tahara, Felipe Navarete, Alicia J. Kowaltowski,
Reactive oxygen species are a by-product of mitochondrial oxidative phosphorylation, derived from a small quantity of superoxide radicals generated during electron transport. We conducted a comprehensive and quantitative study of oxygen consumption, inner membrane potentials, and H(2)O(2) release in mitochondria isolated from rat brain, heart, kidney, liver, and skeletal muscle, using various respiratory substrates (alpha-ketoglutarate, glutamate, succinate, glycerol phosphate, and palmitoyl carnitine). ...
Tópico(s): ATP Synthase and ATPases Research
2009 - Elsevier BV | Free Radical Biology and Medicine
Pierre Dos Santos, Alicia J. Kowaltowski, M. Laclau, Subramanian Seetharaman, Petr Pauček, Sihem Boudina, J.-B. Thambo, Liliane Tariosse, Keith Garlid,
Diazoxide opening of the mitochondrial ATP-sensitive K(+) (mitoK(ATP)) channel protects the heart against ischemia-reperfusion injury by unknown mechanisms. We investigated the mechanisms by which mitoK(ATP) channel opening may act as an end effector of cardioprotection in the perfused rat heart model, in permeabilized fibers, and in rat heart mitochondria. We show that diazoxide pretreatment preserves the normal low outer membrane permeability to nucleotides and cytochrome c and that these beneficial ...
Tópico(s): Cardiac electrophysiology and arrhythmias
2002 - American Physical Society | AJP Heart and Circulatory Physiology
Produção Nacional
Revisado por pares
Alicia J. Kowaltowski, Ricardo G. Cosso, Cláudia Barbosa Ladeira de Campos, Gary Fiskum,
Overexpression of the antiapoptotic Bcl-2 protein enhances the uptake of fluorimetric dyes sensitive to mitochondrial membrane potential, suggesting that Bcl-2 changes the mitochondrial proton gradient. In this study, we performed calibrated measurements of mitochondrial respiration, membrane potential, ΔpH, and intramitochondrial [K+] in digitonin-permeabilized PC12 and GT1-7 neural cells that either do not express human Bcl-2 (control transfectants) or that were transfected with and overexpressed ...
Tópico(s): Neuroscience and Neuropharmacology Research
2002 - Elsevier BV | Journal of Biological Chemistry
Produção Nacional
Revisado por pares
Eduardo Belisle, Alicia J. Kowaltowski,
Tópico(s): Anesthesia and Neurotoxicity Research
2002 - Springer Science+Business Media | Journal of Bioenergetics and Biomembranes
Produção Nacional
Revisado por pares
L.E.S. Netto, Alicia J. Kowaltowski, Roger F. Castilho, Anı́bal E. Vercesi,
Mitochondria constantly generate reactive oxygen species (ROS), which can damage their lipids, proteins, DNA, and RNA. The respiratory chain, responsible for a constant generation of ROS within the cell, is located mostly within the inner mitochondrial membrane. Much of the oxidative damage affecting mitochondrial membrane proteins involves thiol oxidation. Thiol oxidation can result in mitochondrial impairment secondary to respiratory chain inhibition or to opening of a nonselective pore in the ...
Tópico(s): Mitochondrial Function and Pathology
2002 - Academic Press | Methods in enzymology on CD-ROM/Methods in enzymology
Produção Nacional
Revisado por pares
D Cancherini, Leonardo G. Trabuco, Nancy Amaral Rebouças, Alicia J. Kowaltowski,
Isolated kidney mitochondria swell when incubated in hyposmotic solutions containing K + salts in a manner inhibited by ATP, ADP, 5-hydroxydecanoate, and glibenclamide and stimulated by GTP and diazoxide. These results suggest the existence of ATP-sensitive K + channels in these mitochondria, similar to those previously described in heart, liver, and brain. Renal mitochondrial ATP-sensitive K + uptake rates are ∼140 nmol·min –1 ·mg protein –1 . This K + transport results in a slight increase in respiration and ...
Tópico(s): ATP Synthase and ATPases Research
2003 - American Physical Society | AJP Renal Physiology
Produção Nacional
Revisado por pares
Mirian M. da Silva, Adriano Sartori, Eduardo Belisle, Alicia J. Kowaltowski,
Ischemic preconditioning, or the protective effect of short ischemic episodes on a longer, potentially injurious, ischemic period, is prevented by antagonists of mitochondrial ATP-sensitive K + channels (mitoK ATP ) and involves changes in mitochondrial energy metabolism and reactive oxygen release after ischemia. However, the effects of ischemic preconditioning itself on mitochondria are still poorly understood. We determined the effects of ischemic preconditioning on isolated heart mitochondria and ...
Tópico(s): Cardiac Arrest and Resuscitation
2003 - American Physical Society | AJP Heart and Circulatory Physiology
Produção Nacional
Revisado por pares
Renato Ferranti, Mirian M. da Silva, Alicia J. Kowaltowski,
Mitochondrial ATP‐sensitive K + channel (mitoK ATP ) opening was shown previously to slightly increase respiration and decrease the membrane potential by stimulating K + cycling across the inner membrane. Here we show that mitoK ATP opening reduces reactive oxygen species generation in heart, liver and brain mitochondria. Decreased H 2 O 2 release is observed when mitoK ATP is active both with respiration stimulated by oxidative phosphorylation and when ATP synthesis is inhibited. In addition, decreased ...
Tópico(s): ATP Synthase and ATPases Research
2003 - Wiley | FEBS Letters
Produção Nacional
Revisado por pares
Camille C. Caldeira da Silva, Fernanda M. Cerqueira, Lívea Fujita Barbosa, Marisa Helena Gennari de Medeiros, Alicia J. Kowaltowski,
Caloric restriction is the most effective non-genetic intervention to enhance lifespan known to date. A major research interest has been the development of therapeutic strategies capable of promoting the beneficial results of this dietary regimen. In this sense, we propose that compounds that decrease the efficiency of energy conversion, such as mitochondrial uncouplers, can be caloric restriction mimetics. Treatment of mice with low doses of the protonophore 2,4-dinitrophenol promotes enhanced ...
Tópico(s): Mitochondrial Function and Pathology
2008 - Wiley | Aging Cell
Produção Nacional
Revisado por pares
Maynara Fornazari, Juliana G. de Paula, Roger F. Castilho, Alicia J. Kowaltowski,
Brain mitochondrial ATP-sensitive K+ channel (mitoK(ATP)) opening by diazoxide protects against ischemic damage and excitotoxic cell death. Here we studied the redox properties of brain mitoK(ATP) . MitoK(ATP) activation during excitotoxicity in cultured cerebellar granule neurons prevented the accumulation of reactive oxygen species (ROS) and cell death. Furthermore, mitoK(ATP) activation in isolated brain mitochondria significantly prevented H2O2 release by these organelles but did not change Ca2+ ...
Tópico(s): Fuel Cells and Related Materials
2008 - Wiley | Journal of Neuroscience Research
Produção Nacional
Revisado por pares
Raquel Carreira, Pedro Monteiro, Alicia J. Kowaltowski, Lino Gonçalves, Luís A. Providência,
Tópico(s): Fuel Cells and Related Materials
2008 - Springer Science+Business Media | Journal of Bioenergetics and Biomembranes
João Wosniak, Célio X.C. Santos, Alicia J. Kowaltowski, Francisco Rafael Martins Laurindo,
Tópico(s): Cardiac Ischemia and Reperfusion
2008 - Mary Ann Liebert, Inc. | Antioxidants and Redox Signaling
Produção Nacional
Revisado por pares
Graciele Almeida de Oliveira, Erich Birelli Tahara, Andreas Gombert, Mário H. Barros, Alicia J. Kowaltowski,
Tópico(s): Fungal and yeast genetics research
2008 - Springer Science+Business Media | Journal of Bioenergetics and Biomembranes
Produção Nacional
Revisado por pares
Ariel R. Cardoso, João Victor Cabral‐Costa, Alicia J. Kowaltowski,
Tópico(s): Diet, Metabolism, and Disease
2010 - Springer Science+Business Media | Journal of Bioenergetics and Biomembranes
Produção Nacional
Revisado por pares
Fernanda M. Cerqueira, Alicia J. Kowaltowski,
Undernutrition without malnutrition is an intervention that enhances laboratory animal life span, and is widely studied to uncover factors limiting longevity. In a search of the literature over a course of four years, we found that most protocols currently adopted as caloric restriction do not meet micronutrient standards set by the National Research Council for laboratory rats and mice. We provide evidence that the most commonly adopted caloric restriction protocol, a 40% restriction of the AIN- ...
Tópico(s): Genetics, Aging, and Longevity in Model Organisms
2010 - Elsevier BV | Ageing Research Reviews
Produção Nacional
Revisado por pares
Cleverson Busso, Erich Birelli Tahara, Renata Ogusucu, Ohára Augusto, José Ribamar Ferreira‐Junior, Alexander Tzagoloff, Alicia J. Kowaltowski, Mário H. Barros,
Deletion of COQ10 in Saccharomyces cerevisiae elicits a respiratory defect characterized by the absence of cytochrome c reduction, which is correctable by the addition of exogenous diffusible coenzyme Q(2). Unlike other coq mutants with hampered coenzyme Q(6) (Q(6) ) synthesis, coq10 mutants have near wild-type concentrations of Q(6). In the present study, we used Q-cycle inhibitors of the coenzyme QH(2)-cytochrome c reductase complex to assess the electron transfer properties of coq10 cells. Our results ...
Tópico(s): Free Radicals and Antioxidants
2010 - Wiley | FEBS Journal
Produção Nacional
Revisado por pares
Mário H. Barros, Fernanda Marques da Cunha, Graciele Almeida de Oliveira, Erich Birelli Tahara, Alicia J. Kowaltowski,
Despite the fact that ageing necessarily displays unique aspects in a single-cell organism, yeast, in particular Saccharomyces cerevisiae, are useful as model organisms to study ageing. Here we review mitochondrial characteristics involved in yeast longevity, including biogenesis, autophagy, respiration and oxidative phosphorylation, nutrient sensing, mitochondria-nuclear signaling, redox state and mitochondrial DNA integrity. Altogether, the yeast model unearths a rich and complex network involving ...
Tópico(s): Fungal and yeast genetics research
2010 - Elsevier BV | Mechanisms of Ageing and Development
Produção Nacional
Revisado por pares
Ariel R. Cardoso, Bruno B. Queliconi, Alicia J. Kowaltowski,
Mitochondria are the central coordinators of energy metabolism and alterations in their function and number have long been associated with metabolic disorders such as obesity, diabetes and hyperlipidemias. Since oxidative phosphorylation requires an electrochemical gradient across the inner mitochondrial membrane, ion channels in this membrane certainly must play an important role in the regulation of energy metabolism. However, in many experimental settings, the relationship between the activity ...
Tópico(s): Metabolomics and Mass Spectrometry Studies
2010 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Bioenergetics
Produção Nacional
Revisado por pares
Bruno B. Queliconi, Andrew P. Wojtovich, Sergiy M. Nadtochiy, Alicia J. Kowaltowski, Paul S. Brookes,
The mitochondrial ATP-sensitive potassium channel (mKATP) is important in the protective mechanism of ischemic preconditioning (IPC). The channel is reportedly sensitive to reactive oxygen and nitrogen species, and the aim of this study was to compare such species in parallel, to build a more comprehensive picture of mKATP regulation. mKATP activity was measured by both osmotic swelling and Tl+ flux assays, in isolated rat heart mitochondria. An isolated adult rat cardiomyocyte model of ischemia–reperfusion ( ...
Tópico(s): Cardiac Arrest and Resuscitation
2010 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Produção Nacional
Revisado por pares
Fábio Monaro Engelmann, Ildemar Mayer, Dino Santesso Gabrielli, Henrique E. Toma, Alicia J. Kowaltowski, Koiti Araki, Maurı́cio S. Baptista,
Tópico(s): Nanoplatforms for cancer theranostics
2007 - Springer Science+Business Media | Journal of Bioenergetics and Biomembranes
Produção Nacional
Revisado por pares
Heberty Tarso Facundo, Juliana G. de Paula, Alicia J. Kowaltowski,
Pharmacological mitochondrial ATP-sensitive K+ channel (mitoKATP) opening protects against ischemic damage and mimics ischemic preconditioning. However, physiological and pathological signaling events that open this channel are still not fully understood. We found that catalase, which removes H2O2, is capable of reversing the beneficial effects of ischemic preconditioning but not of mitoKATP agonist diazoxide. On the other hand, 2-mercaptopropionylglycine prevented cardioprotection in both cases, ...
Tópico(s): Fuel Cells and Related Materials
2007 - Elsevier BV | Free Radical Biology and Medicine
Produção Nacional
Revisado por pares
Anı́bal E. Vercesi, Roger F. Castilho, Alicia J. Kowaltowski, Helena C.F. Oliveira,
Changes in mitochondrial function are intimately associated with metabolic diseases. Here, we review recent evidence relating alterations in mitochondrial energy metabolism, ion transport and redox state in hypercholesterolemia and hypertriglyceridemia. We focus mainly on changes in mitochondrial respiration, K(+) and Ca(2+) transport, reactive oxygen species generation and susceptibility to mitochondrial permeability transition.
Tópico(s): Metabolism and Genetic Disorders
2007 - Wiley | IUBMB Life
Produção Nacional
Revisado por pares
Calorie restriction (CR) is well established to enhance the lifespan of a wide variety of organisms, although the mechanisms are still being uncovered. Recently, some authors have suggested that CR acts through hormesis, enhancing the production of reactive oxygen species (ROS), activating stress response pathways, and increasing lifespan. Here, we review the literature on the effects of CR and redox state. We find that there is no evidence in rodent models of CR that an increase in ROS production ...
Tópico(s): Spaceflight effects on biology
2011 - Taylor & Francis | Redox Report
Produção Nacional
Revisado por pares
Maynara Fornazari, Isis C. Nascimento, Arthur A. Nery, Camille C. Caldeira da Silva, Alicia J. Kowaltowski, Henning Ulrich,
Tópico(s): Adipose Tissue and Metabolism
2011 - Springer Science+Business Media | Journal of Bioenergetics and Biomembranes