Kazuki Omoteyama, Minoru Takagi,
... lentiviral particles on chondrocyte marker gene expression and alkaline phosphatase (ALP) activity were investigated. At confluency, gene silencing down-regulated expression of the Sox trio (Sox5, 6, 9), Dlx5 and Alp mRNA, as ...
Tópico(s): NF-κB Signaling Pathways
2010 - Elsevier BV | Biochemical and Biophysical Research Communications
... alpha-chloro-1,2-dideoxy-3,4,6-trio-O-acetyl-D-glucopyranose with appropriately protected 15 alpha-hydroxyestrogens by the Koenigs-Knorr reaction employing cadmium carbonate as a catalyst. Subsequent removal of protecting groups with methanolic potassium hydroxide provided the desired conjugates. 15 alpha-Hydroxyestrone 15-N-acetylglucosaminide was synthesized from the corresponding 15 alpha-hydroxyestradiol derivative by Jones oxidation followed by brief alkaline hydrolysis. These conjugates underwent enzymatic hydrolysis with beta- ...
Tópico(s): Natural product bioactivities and synthesis
1995 - Elsevier BV | Steroids
Erinç Bahçegül, Erinç Bahçegül, Necati Özkan,
... is the most popular member of the biopolymer trio for material applications, but despite constituting around half of a lignocellulosic biomass, attention hemicellulose and lignin receive is restricted in this sense, which gets even more limited when it comes to their utilization for 3D printing. A novel practical procedure is developed to address this issue, which enables the 3D printing of the alkaline soluble portion of corn cobs in its crude ...
Tópico(s): biodegradable polymer synthesis and properties
2022 - Elsevier BV | Industrial Crops and Products
Martha Rook, Scott Delach, Galina Deyneko, Andrew Worlock, Jia Liu Wolfe,
... STR accurate genotype calling rates were achieved from alkaline lysis of ∼1500 cells, whereas proteinase K digestion rendered effective performance with as few as 100 cells. Isolated human DNA samples from six cell lines representing two sets of trios (mother, father, and offspring) were obtained from the ...
Tópico(s): DNA Repair Mechanisms
2004 - Elsevier BV | American Journal Of Pathology
Rhea Arya, Brittany C. Hudson, Tracey Dawson Cruz,
... buffer, and urea/thiourea buffer. Quantification using Quantifiler® Trio of vaginal and semen lysates revealed that the alkaline (25 mM NaOH) and M-PER™ methods were ...
Tópico(s): Sperm and Testicular Function
2023 - Wiley | Journal of Forensic Sciences
Anthony L. Hinrichs, Jen C. Wang, Bernd Bufe, Jennifer M. Kwon, John Budde, Rebecca Allen, Sarah Bertelsen, E. Whitney Evans, Danielle M. Dick, John P. Rice, Tatiana Foroud, John I. Nürnberger, Jay A. Tischfield, Samuel Kuperman, Raymond Crowe, Victor Hesselbrock, Marc A. Schuckit, Laura Almasy, Bernice Porjesz, Howard J. Edenberg, Henri Begleiter, Wolfgang Meyerhof, Laura J. Bierut, Alison Goate,
... are of mixed ancestry (by self-report). Additional trios.—The COGA sample contains additional pedigrees with cell ... the linkage sample. From these, we identified 85 trios consisting of a DSM-IV–defined alcohol-dependent individual and two parents. This sample of “additional trios,” including five African American trios, was typed for SNP rs846664. Nonparametric multipoint linkage ... examine the consistency of results. For the additional trios, association was first tested, using Transmit, in this ... genotyped this SNP in an independent sample: 85 trios (consisting of a DSM-IV alcohol-dependent individual ...
Tópico(s): Advanced Chemical Sensor Technologies
2005 - Elsevier BV | The American Journal of Human Genetics
Oliver Semler, Lutz Garbes, Katharina Keupp, Daniel Swan, Katharina Zimmermann, Jutta Becker, Sandra Iden, Brunhilde Wirth, Peer Eysel, Friederike Koerber, Eckhard Schöenau, Stefan K. Bohlander, Bernd Wollnik, Christian Netzer,
... violations of the Mendelian inheritance rules in the trio by using GATK SelectVariants.21DePristo M.A. Banks ... proband. We confirmed paternity in both proband-parent trios by genotyping 11 microsatellite markers (data not shown; correct paternity in the first proband-parent trio can also be derived from the low number ...
Tópico(s): Ubiquitin and proteasome pathways
2012 - Elsevier BV | The American Journal of Human Genetics
Hans Hölschermann, Rainer M. Bohle, Hagen Zeller, Heiko Schmidt, U. Stahl, Ludger Fink, Helmut Grimm, Harald Tillmanns, Werner Haberbosch,
... minutes at 99°C, were performed using a TRIO Thermoblock (Biometra, Göttingen, Germany). cDNA was stored at − ... contained 1.5 mmol/L MgCl2. PCR conditions (TRIO Thermoblock, Biometra) for cell-picking templates were 94° ...
Tópico(s): Platelet Disorders and Treatments
1999 - Elsevier BV | American Journal Of Pathology
Philip M. Smallwood, John C. Williams, Qiang Xu, Daniel J. Leahy, Jeremy Nathans,
... pairs of cysteines that form the highly conserved trio of disulfide bonds shared among all cystine knot ... pairs of cysteines that form the highly conserved trio of disulfide bonds shared among all cystine knot ... PBS, phosphate-buffered saline; WT, wild type; AP, alkaline phosphatase; Xwnt8, Xenopus Wnt8; GPI, glycosylphosphatidylinositol. 2The abbreviations ... PBS, phosphate-buffered saline; WT, wild type; AP, alkaline phosphatase; Xwnt8, Xenopus Wnt8; GPI, glycosylphosphatidylinositol. (9Bhanot P. ... 65 °C for 90 min to inactivate endogenous alkaline phosphatase (AP). Immobilized AP was visualized with nitro ...
Tópico(s): Genetic Syndromes and Imprinting
2006 - Elsevier BV | Journal of Biological Chemistry
Nalini Balendran, R. Lee Clough, J. Rafael Argüello, Ruth Barber, Colin Veal, Andrew B. Jones, Jane L. Rosbotham, Anne-Margaret Little, J. Alejandro Madrigal, Juliet N. Barker, Stephen H. Powis, Richard C. Trembath,
... affected relative pairs (non sib)18Total number of trios with both parents available146Total number of "trios" with one parent available38Number of fully independent trios77Total ... stringent washing, detection was achieved using anti-digoxigenin alkaline phosphatase Fab fragments (Boehringer Mannheim GmbH, Germany). HLA- ...
Tópico(s): T-cell and B-cell Immunology
1999 - Elsevier BV | Journal of Investigative Dermatology
Alistair T. Pagnamenta, Yoshiko Murakami, John Taylor, Consuelo Anzilotti, Malcolm F. Howard, Venessa Miller, Diana Johnson, Shereen Tadros, Sahar Mansour, I. Karen Temple, Rachel Firth, Elisabeth Rosser, Rachel Harrison, Bronwen Kerr, Niko Popitsch, Taroh Kinoshita, Jenny C. Taylor, Usha Kini,
... analysed the exome data from 4293 parent-child trios recruited to the Deciphering Developmental Disorders (DDD) study. ... with them being causative. In four families, abnormal alkaline phosphatase results were observed in the direction expected. ...
Tópico(s): Cellular transport and secretion
2017 - Springer Nature | European Journal of Human Genetics
Beatrice Hoyos, Sulin Jiang, Ulrich Hämmerling,
... Phe-7, Phe-8, and Trp-22. This trio was absent from the αC1B domain, explaining why ... Phe-7, Phe-8, and Trp-22. This trio was absent from the αC1B domain, explaining why ... FLAG® M2-agarose affinity gel, anti-rabbit IgG-alkaline phosphatase conjugate, and epidermal growth factor (EGF) were ...
Tópico(s): Antioxidant Activity and Oxidative Stress
2005 - Elsevier BV | Journal of Biological Chemistry
Johannes Schumacher, Heidi Anthoni, Faten Dahdouh, Inke R. König, Axel M. Hillmer, Nadine Kluck, Malou Manthey, Ellen Plume, Andreas Warnke, Helmut Remschmidt, Jutta Hülsmann, Sven Cichon, Cecilia M. Lindgren, Peter Propping, Marco Zucchelli, Andreas Ziegler, Myriam Peyrard‐Janvid, Gerd Schulte‐Körne, Markus M. Nöthen, Juha Kere,
... risks and allele frequencies from the case parent trio design for candidate-gene studies.Hum Hered. 2002; ... relative risks and attributable risks from case parent trio design for candidate-gene studies.Hum Hered. 2005; ...
Tópico(s): Autism Spectrum Disorder Research
2005 - Elsevier BV | The American Journal of Human Genetics
Jesusa L. Rosales, J. Ernst, Janice L. Hallows, Ki‐Young Lee,
... in pituitary cells, Cdk5 phosphorylation of the RhoGEF, Trio, is also involved in the actin dynamics required ... Eipper B.A. Mains R.E. Cdk5 and Trio modulate endocrine cell exocytosis.J. Cell Sci. 2004; ...
Tópico(s): Neurogenesis and neuroplasticity mechanisms
2004 - Elsevier BV | Journal of Biological Chemistry
Marianna Parlato, Fabienne Charbit‐Henrion, Jie Pan, Claudio Romano, Rémi Duclaux‐Loras, Marie‐Hélène Le Du, Neil Warner, Paola Francalanci, Julie Bruneau, Marc Bras, Mohammed Zarhrate, Bernadette Bègue, Nicolas Guégan, Sabine Rakotobé, Nathalie Kapel, Paola De Angelis, Anne M. Griffiths, Karoline Fiedler, Eileen Crowley, Frank M. Ruemmele, Aleixo M. Muise, Nadine Cerf–Bensussan,
... by WES Whole-exome sequencing was performed on trios including affected individuals and their unaffected parents. Annotated ... heterozygous mutations in the ALPI gene, encoding intestinal alkaline phosphatase, in both patients (Fig 2A and B, ... based on the crystal structure of human placental alkaline phosphatase (ALPP), which shares 87% identity with ALPI ( ...
Tópico(s): Galectins and Cancer Biology
2018 - Springer Nature | EMBO Molecular Medicine
Chitra Rajagopal, Kathryn L. Stone, Victor P. Francone, Richard E. Mains, Betty Eipper,
... Scholar). Cytosolic PAM-CD interactors include Kalirin and Trio, Rho GDP/GTP exchange factors that affect cytoskeletal ... extracts (150 μg) were treated with calf intestinal alkaline phosphatase (CIP; 30 units; New England BioLabs) at ...
Tópico(s): Lipid Membrane Structure and Behavior
2009 - Elsevier BV | Journal of Biological Chemistry
Chun-Gyu Kim, Tin‐Wein Yu, Craig B. Fryhle, Sandeep Handa, Heinz G. Floss,
... spectra (ESI-MS) were acquired using a Fisons Trio 2000 mass spectrometer. Amycolatopsis mediterranei S699, a gift ... about 23 μCi of tritium, was incubated with alkaline phosphatase and then subjected to preparative layer chromatography ( ... phosphopyridoxyl-AHBA was then dephosphorylated by incubation with alkaline phosphatase and the N-pyridoxyl-AHBA purified by ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
1998 - Elsevier BV | Journal of Biological Chemistry
Rakesh Kumar Singh, Chintan Patel, David Trio, Eric McNamara, Christopher Wargo,
... processes. The effects of extended exposure of one alkaline and four acidic PCMP cleaning chemistries on brush ... tested acidic cleans caused significant discoloration, whereas the alkaline clean caused minimal change. Further, all the tested ...
Tópico(s): Copper Interconnects and Reliability
2010 - Institute of Physics | ECS Transactions
Sylke Gellrich, Sascha Rutz, Sven Golembowski, C.Y. onder red. van E. Jacobs, Malgorzata von Zimmermann, Pamela Lorenz, Heike Audring, Marcus Muche, Wolfram Sterry, Sigbert Jahn,
... round cycle program was carried out on a TRIO-Thermoblock (Biometra) and consisted of one cycle at ...
Tópico(s): Nail Diseases and Treatments
2001 - Elsevier BV | Journal of Investigative Dermatology
... la lumière solaire pour modifier les étres des trios règnes de la nature et surtout ceux du ...
Tópico(s): Plant Water Relations and Carbon Dynamics
2008 - Ecological Society of America | Bulletin of the Ecological Society of America
Daniel C. Lee, Jimin Zheng, Yi‐Min She, Zongchao Jia,
... active site, blocking the magnesium-binding aspartic acid trio (D567, D569 and D647, Walker motifs A′ and ... uncovered. First, when Etk samples were treated with alkaline phosphatase, the protein precipitated immediately and irreversibly (Supplementary ...
Tópico(s): Bacteriophages and microbial interactions
2008 - Springer Nature | The EMBO Journal
A Romanko, Nazim A. Imamverdiyev, V. Yu. Prokofiev, I. V. Vikentiev, Bahman Rashidi, Alexander Savichev, M Heidari,
... metallogeny of East Iran led by outstanding regional trio: E. Romanko, A. Houshmandzadeh, and M.A.A. Nogole-Sadat).Geological northeastern (NE) zoning and "hot" tectonics due to the African superplume activity including, probably, known delamination of lithosphere during collision of mantle lithosphere ca. 13 Ma is principal here.Intraplate alkaline-subalkaline rocks of the region studied including Quaternary ...
Tópico(s): earthquake and tectonic studies
2018 - | International Journal of Mining Science
Joni H. Hansson, Suzanne Watnick,
... 02352.5Low BicaVera (FMC)Glucose358-5117.42034Low Gambrosol Trio (Gambro)Glucose357-4836.33400Low Physioneal (Baxter)Glucose344-5837. ... lactate, bicarbonate, or both) and electrolytes at an alkaline pH. Before use, the compartments are mixed, resulting ...
Tópico(s): Muscle and Compartmental Disorders
2015 - Elsevier BV | American Journal of Kidney Diseases
Thomas Morley, Lisa M. Willis, Chris Whitfield, Warren W. Wakarchuk, Stephen G. Withers,
... residues as the general acid/base, and a trio of arginines associated with the sialic acid carboxylate. ... II (2.8 mg/ml, 200 μl) and alkaline phosphatase (130 units/μl, 2 μl) for 2 ...
Tópico(s): Genomics and Phylogenetic Studies
2009 - Elsevier BV | Journal of Biological Chemistry
Alexej Knaus, Fanny Kortüm, Tjitske Kleefstra, Asbjørg Stray‐Pedersen, Dejan Đukić, Yoshiko Murakami, Thorsten Gerstner, Hans van Bokhoven, Zafar Iqbal, Denise Horn, Taroh Kinoshita, Maja Hempel, Peter Krawitz,
... al.DDD StudyAnalysis of exome data for 4293 trios suggests GPI-anchor biogenesis defects are a rare ...
Tópico(s): Glycosylation and Glycoproteins Research
2019 - Elsevier BV | The American Journal of Human Genetics
Gintaras Deikus, Ciarán Condon, David H. Bechhofer,
... Storm 860 PhosphorImager (GE Healthcare) or a Typhoon TRIO variable mode imager (GE Healthcare). PNPase Binding Assay— ...
Tópico(s): Bacteriophages and microbial interactions
2008 - Elsevier BV | Journal of Biological Chemistry
Hiroko Beck, William E. Boden, Sushmitha Patibandla, Dmitriy Kireyev, Vipul Gupta, Franklin Campagna, Michael Cain, Joseph E. Marine,
... in the patient's abdomen.Figure 3The "Bowtie Trio": William Chardack, Andrew Gage, and Wilson Greatbatch at ... 5 years, compared to a similar loss for alkaline cells over only 1 year. Lithium systems are ...
Tópico(s): Cardiac Arrhythmias and Treatments
2010 - Elsevier BV | The American Journal of Cardiology
Ali R. Mani, Silvia Ippolito, José C. Moreno, Theo J. Visser, Kevin P. Moore,
... mm film thickness) interfaced with a mass spectrometer (Trio 1000; Fisons Instruments, Beverly, MA). The ion source ... μg) were added to the samples prior to alkaline hydrolysis. Following two steps of solid phase extraction, ...
Tópico(s): Glutathione Transferases and Polymorphisms
2007 - Elsevier BV | Journal of Biological Chemistry
Simone Martinelli, Oliver H.F. Krumbach, Francesca Pantaleoni, Simona Coppola, Ehsan Amin, Luca Pannone, Kazem Nouri, Luciapia Farina, Radovan Dvorský, Francesca Romana Lepri, Marcel Buchholzer, Raphael Konopatzki, Laurence E. Walsh, Katelyn Payne, Mary Ella Pierpont, Samantha A. Schrier Vergano, Katherine G. Langley, Douglas P. Larsen, Kelly D. Farwell, Sha Tang, Cameron Mroske, Ivan Gallotta, Elia Di Schiavi, Matteo Della Monica, Licia Lugli, Cesare Rossi, Marco Seri, Guido Cocchi, Lindsay B. Henderson, Berivan Baskin, Mariëlle Alders, Roberto Mendoza‐Londono, Lucie Dupuis, Deborah A. Nickerson, Jessica X. Chong, Naomi Meeks, Kathleen Brown, Tahnee N. Causey, Megan T. Cho, Stephanie Demuth, M. Cristina Digilio, Bruce D. Gelb, Michael J. Bamshad, Martin Zenker, Mohammad Reza Ahmadian, Raoul C. M. Hennekam, Marco Tartaglia, Ghayda Mirzaa,
... leukocytes or saliva specimens, and a child-parent trio-based strategy. Exome capture was carried out using ... fluorescent nucleotide-bound CDC42 variants were prepared using alkaline phosphatase (Roche) and phosphodiesterase (Sigma Aldrich) at 4° ...
Tópico(s): Congenital heart defects research
2018 - Elsevier BV | The American Journal of Human Genetics
Boel Lanne, Britt‐Marie Olsson, Per- Jovall, Jonas öm, Henrik Linder, Britt‐Inger Marklund, Jörgen Bergström, Karl‐Anders Karlsson,
... of partially methylated alditol acetates, a quadropole MS (Trio-II, VG Masslab, Altricham, UK) was used. The ...
Tópico(s): Drug Transport and Resistance Mechanisms
1995 - Elsevier BV | Journal of Biological Chemistry