M. Collier, Chris O’Neill, Alaina J. Ammit, D. M. Saunders,
... on: Oxford Academic PubMed Google Scholar A. J. Ammit, A. J. Ammit Human Reproduction Unit Royal North Shore Hospital of ...
Tópico(s): Assisted Reproductive Technology and Twin Pregnancy
1990 - Oxford University Press | Human Reproduction
Peter Bradding, Andrew F. Walls, Stephen T. Holgate,
... ligands CXCL9, CXCL10, and CXCL11.14Brightling C.E. Ammit A.J. Kaur D. Black J.L. Wardlaw ... asthmatic compared with normal ASM.14Brightling C.E. Ammit A.J. Kaur D. Black J.L. Wardlaw ... with the surrounding airway mucosa.14Brightling C.E. Ammit A.J. Kaur D. Black J.L. Wardlaw ...
Tópico(s): Allergic Rhinitis and Sensitization
2006 - Elsevier BV | Journal of Allergy and Clinical Immunology
Alaina J. Ammit, Xanthe E. Wells, C. O’Neill,
... by murine preimplantation embryos Get access A.J. Ammit, A.J. Ammit Human Reproduction Unit, Royal North Shore Hospital of ...
Tópico(s): Reproductive Biology and Fertility
1992 - Oxford University Press | Human Reproduction
C. O’Neill, M. Collier, Douglas M. Saunders,
... 147–56. 9 Spinks, R. N., A. J. Ammit, D. M. Saunders & C. O'Neill. 1986. Embryo- ... Baldo, B. A., M. A. Smal, A. J. Ammit, D. M. Saunders & C. O'Neill. 1986. Further ...
Tópico(s): Gynecological conditions and treatments
1988 - Wiley | Annals of the New York Academy of Sciences
Danielle Morse, Søren Erik Pischke, Zhihong Zhou, Roger J. Davis, Richard A. Flavell, Torsten Loop, Sherrie L. Otterbein, Leo E. Otterbein, Augustine M.K. Choi,
... constructs were kindly provided by Drs. Alaina J. Ammit (Faculty of Pharmacy, University of Sydney, Sydney, Australia) ... We thank Drs. Oliver Eikelberg and Alaina J. Ammit for generously donating the IL-6 promoter constructs.
Tópico(s): Optimism, Hope, and Well-being
2003 - Elsevier BV | Journal of Biological Chemistry
Christopher E. Brightling, Peter Bradding, Ian Pavord, Andrew J. Wardlaw,
... Clin Exp Allergy 1993; 23: 919– 25. 64 Ammit AJ, Bekir SS, Johnson PR, Hughes JM, Armour ... Allergy 2000; 30: 606– 14. 89 Page S, Ammit AJ, Black JL, Armour CL. Human mast cell ...
Tópico(s): Respiratory and Cough-Related Research
2003 - Wiley | Clinical & Experimental Allergy
Aili L. Lazaar, Reynold A. Panettieri,
... promote maximal ASM growth responses.7Krymskaya V.P. Ammit A.J. Hoffman R.K. Eszterhas A.J. ... ASM cells.39Krymskaya V.P. Goncharova E.A. Ammit A.J. Lim P.N. Goncharov D.A. ...
Tópico(s): Helicobacter pylori-related gastroenterology studies
2005 - Elsevier BV | Journal of Allergy and Clinical Immunology
Alaina J. Ammit, Carol Armour, JUDITH L. BLACK,
... responsible for enhanced contractile reactivity in sensitized tissue. Ammit AJ, Armour CL, Black JL. Smooth-muscle myosin ...
Tópico(s): Cell Adhesion Molecules Research
2000 - American Thoracic Society | American Journal of Respiratory and Critical Care Medicine
William E. Roudebush, Joe B. Massey, Hilton I. Kort, Carlene W. Elsner, Andrew A. Toledo, Daniel B. Shapiro,
... PubMed Scopus (119) Google Scholar, 4Pike I.L. Ammit A.J. O’Neill C. Actions of platelet ... embryo development (11Ryan J.P. O’Neill C. Ammit A.J. Roberts C.G. Metabolic and developmental ... potential (12O’Neill C. Gidley-Baird A.A. Ammit A.J. Saunders D.M. Use of a ... potential (12O’Neill C. Gidley-Baird A.A. Ammit A.J. Saunders D.M. Use of a ... J.P. Spinks N.R. O’Neill C. Ammit A.J. Wales R.G. Platelet activating factor ( ...
Tópico(s): Pregnancy and preeclampsia studies
2003 - Elsevier BV | Fertility and Sterility
John D. O’Neil, Alaina J. Ammit, Andrew R. Clark,
Tristetraprolin (TTP) is an RNA-destabilizing protein that exerts profound anti-inflammatory effects by inhibiting the expression of tumour necrosis factor and many other inflammatory mediators. The mitogen-activated protein kinase (MAPK) p38 signaling pathway controls the strength and duration of inflammatory responses by regulating both the expression and function of TTP. The kinase MK2 (MAPK activated kinase 2) is activated by MAPK p38, and in turn phosphorylates TTP at two critical serine residues. ...
Tópico(s): NF-κB Signaling Pathways
2017 - Elsevier BV | The International Journal of Biochemistry & Cell Biology
C. O’Neill, M. Collier, Alaina J. Ammit, John P. Ryan, D. M. Saunders, Ian Pike,
The supplementation of culture medium with platelet activating factor (PAF) on the subsequent implantation and pregnancy potential of pre-embryos produced by in-vitro fertilisation was studied. Pre-embryo culture medium was supplemented with 0 (control), 0.186, 0.93, or 1.49 mumol/1 PAF. Pre-embryos were transferred to PAF-containing medium 15-17 h after insemination (ie, just before syngamy) for 24 h and then transferred to the uterus. For 185 women receiving control pre-embryos, the pregnancy rate ( ...
Tópico(s): Periodontal Regeneration and Treatments
1989 - Elsevier BV | The Lancet
John P. Ryan, N. R. Spinks, C. O’Neill, Alaina J. Ammit, R. G. WALES,
Abstract Factors affecting the production of platelet activating factor (PAF) by mouse embryos during culture in vitro were investigated. Detectable levels of embryo‐derived PAF were produced within 1–4 hr with maximum PAF activity being observed after 6 hr of culture in vitro. The amount of PAF detected in media after 24 hr of culture of two‐cell embryos was equivalent to 12.8 ng PAF/embryo. However, differences in activity were apparent with increased time in culture. Reduced synthesis of PAF during ...
Tópico(s): Reproductive Biology and Fertility
1989 - Wiley | Journal of Cellular Biochemistry
M. Collier, Chris O’Neill, Alaina J. Ammit, D. M. Saunders,
The soluble platelet activating factor (PAF) produced by mouse embryos was shown to have properties similar to 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphocholine (PAF-acether). In this study PAF was extracted from the medium In which human embryos were cultured for ∼ 18 h prior to transfer. The extracted embryo-derived PAF moved on silica thin layer chromatograms with the same RF of 0.26 ± 0.03 (n = 26) as PAF-acether. Embryo-derived PAF or PAF-acether activity was assayed by monitoring the decrease in the ...
Tópico(s): Platelet Disorders and Treatments
1988 - Oxford University Press | Human Reproduction
Chiara Parlati, Paolo Colombo, Francesca Buttini, Paul M. Young, Handoko Adi, Alaina J. Ammit, Daniela Traini,
Tópico(s): Asthma and respiratory diseases
2009 - Springer Science+Business Media | Pharmaceutical Research
Accumulating evidence indicates that p38 mitogen-activated protein kinase (MAPK) could play more than one role in Alzheimer's disease (AD) pathophysiology and that patients suffering from AD dementia could benefit from p38 MAPK inhibitors. The p38 MAPK signalling has been widely accepted as a cascade contributing to neuroinflammation. However, deepening insight into the underlying biology of Alzheimer's disease reveals that p38 MAPK operates in other events related to AD, such as excitotoxicity, synaptic ...
Tópico(s): Melanoma and MAPK Pathways
2009 - Elsevier BV | Neuropharmacology
Rania Salama, Daniela Traini, Hak‐Kim Chan, Adrian Sung, Alaina J. Ammit, Paul M. Young,
Abstract A series of microparticle formulations, designed for controlled release pulmonary therapy, were evaluated in terms of their physical properties, aerosol performance, lung epithelial cell toxicity, and controlled release profile. A protein, bovine serum albumin (BSA) was chosen as a model macromolecule active ingredient which was coprocessed, using spray drying, with varying concentrations of the release modifier, polyvinyl alcohol (PVA). The spray dried microparticles were tested for their physico- ...
Tópico(s): Advanced Drug Delivery Systems
2009 - Elsevier BV | Journal of Pharmaceutical Sciences
K. M. Battye, Alaina J. Ammit, Chris O’Neill, G. Evans,
Embryos were collected from superovulated ewes on Day 2 (2-8 cell), Day 4 (8-16 cell) and Day 6 (morula/early blastocyst). Two embryos were cultured in 1 ml of one of four media: (i) Ham's F10 + 4 mg bovine serum albumin (BSA)/ml, (ii) synthetic oviduct fluid medium + 20% human serum, (iii) Quinn's human tubal fluid medium (HTF) + 3 mg BSA/ml or (iv) HTF + 10% acid-treated fetal calf serum for 24 h. They were transferred to fresh media of the same type and their further development was monitored. A quantitative ...
Tópico(s): Pregnancy and preeclampsia studies
1991 - Bioscientifica | Reproduction
Alaina J. Ammit, Chris O’Neill,
A bioassay for the measurement of platelet-activating factor (PAF) based on the quantification of platelet aggregation was developed. The method used a platelet analyzer in conjunction with a multiwelled micromixing device and whole blood collected from male New Zealand White rabbits. This bioassay can be nonselective and used to quantitate platelet aggregation induced by any activator. The EC50 for platelet-activating factor, arachidonic acid, and adenosine diphosphate were 0.0232, 55, and 10 microM, ...
Tópico(s): Nitric Oxide and Endothelin Effects
1991 - Elsevier BV | Journal of Pharmacological Methods
The pre-embryos of a number of species produce platelet-activating factor (PAF). This study compared the sensitivity, reproducibility and cross-reactivity of a radioimmunoassay (RIA) for PAF with a quantitative PAF bioassay and assessed the use of both assays for the measurement of murine and human embryo-derived PAF. PAF was extracted and partially purified by thin-layer chromatography (TLC) from medium in which mouse (30 × 2-cell) or individual human embryos [produced by in-vitro fertilization (IVF)] ...
Tópico(s): Pulmonary Hypertension Research and Treatments
1991 - Oxford University Press | Human Reproduction
Frans P. Nijkamp, Ferdi Engels, Paul A. J. Henricks, Antoon J. van Oosterhout,
... Niimi, Qi Ge, Lyn M. Moir, Alaina J. Ammit, Thomas Trian, Janette K. Burgess, Judith L. Black, ...
Tópico(s): Asthma and respiratory diseases
1992 - American Physiological Society | Physiological Reviews
C. O’Neill, JP Ryan, M. Collier, DM Saunders, Alaina J. Ammit, IL Pike,
Reproduction, Fertility and Development is an international journal publishing original research , review and comment in the fields of reproduction and developmental biology in humans, domestic animals and wildlife
Tópico(s): Assisted Reproductive Technology and Twin Pregnancy
1992 - CSIRO Publishing | Reproduction Fertility and Development
JP Ryan, C. O’Neill, Alaina J. Ammit, CG Roberts,
Platelet activating factor (PAF) is an ether phospholipid produced by preimplantation embryos of a number of species. Production of PAF by embryos has been measured by detecting thrombocytopenia in a splenectomized mouse bioassay, platelet aggregation bioassays in vitro and a specific radioimmunoassay. Production is highly variable and is adversely affected by culture in vitro. It has, however, been correlated with morphology, development rates in vitro and the pregnancy potential of embryos following ...
Tópico(s): Reproductive Biology and Fertility
1992 - CSIRO Publishing | Reproduction Fertility and Development
Elena A. Goncharova, Alaina J. Ammit, Carla Irani, Richard G. Carroll, Andrew Eszterhas, Reynold A. Panettieri, Vera P. Krymskaya,
Human vascular smooth muscle cell proliferation and migration contribute to vascular remodeling in pulmonary hypertension and atherosclerosis. The precise mechanisms that regulate structural remodeling of the vessel wall remain unknown. This study tests the hypothesis that phosphatidylinositol 3-kinase (PI3K) activation is both necessary and sufficient to mediate human pulmonary vascular smooth muscle (PVSM) cell proliferation and migration. Microinjection of human PVSM cells with a dominant-negative ...
Tópico(s): Protein Tyrosine Phosphatases
2002 - American Physical Society | AJP Lung Cellular and Molecular Physiology
Alaina J. Ammit, Aili L. Lazaar, Carla Irani, Geraldine M. O’Neill, Nancy Gordon, Yassine Amrani, Raymond B. Penn, Reynold A. Panettieri,
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Tópico(s): Respiratory viral infections research
2002 - American Thoracic Society | American Journal of Respiratory Cell and Molecular Biology
Fügen Aktan, Sheridan Henness, Basil D. Roufogalis, Alaina J. Ammit,
Gypenosides isolated from Gynostemma pentaphyllum are widely used in traditional Chinese medicine, with beneficial effects reported in numerous diseases, including inflammation and atherosclerosis, although the mechanism underlying these therapeutic effects is unknown. Because increased nitric oxide (NO) plays a role in these pathological conditions, we investigated whether the pharmacological activity of gypenosides is due to suppression of NO synthesis. The markedly increased production of nitrite ...
Tópico(s): Natural product bioactivities and synthesis
2003 - Elsevier BV | Nitric Oxide
Magdalena Karbowniczek, Jane Yu, Elizabeth P. Henske,
... the structural remodeling of vessel walls.30Goncharova EA Ammit AJ Irani C Carroll RG Eszterhas AJ Panettieri ...
Tópico(s): Renal and related cancers
2003 - Elsevier BV | American Journal Of Pathology
Effie Nurtjahja-Tjendraputra, Alaina J. Ammit, Basil D. Roufogalis, Van H. Tran, Colin C. Duke,
Tópico(s): Cholesterol and Lipid Metabolism
2003 - Elsevier BV | Thrombosis Research
Melissa McClean, M Matheson, Karen McKay, Peter R. Johnson, A-C. Rynell, Alaina J. Ammit, Judith L. Black, Norbert Berend,
Breathing at volumes lower than functional residual capacity (FRC) can induce changes in nonasthmatic airways consistent with the behaviour of asthmatic airways. This study investigated the chronic effect of breathing at volumes lower than FRC on the contractility of airway smooth muscle and myosin light chain kinase (MLCK) content and activity. Sheep of three age groups (neonate, adolescent and adult) had their FRC reduced by ∼25% for 4 weeks using a leather corset. Contractile responses to carbachol ...
Tópico(s): Pharmacological Effects and Assays
2003 - European Respiratory Society | European Respiratory Journal
Timo Quante, Yee Ching Ng, Emma E. Ramsay, Sheridan Henness, Jodi C. Allen, Johannes Parmentier, Qi Ge, Alaina J. Ammit,
Section:ChooseTop of pageAbstract <<MATERIALS AND METHODSRESULTSDISCUSSIONReferencesCITING ARTICLES
Tópico(s): Galectins and Cancer Biology
2008 - American Thoracic Society | American Journal of Respiratory Cell and Molecular Biology
Sung-Yoon Jo, Amandeep Bajwa, Alaa S. Awad, Kevin R. Lynch, Mark D. Okusa,
... in bronchoalveolar lavage fluid of asthmatic patients.74.Ammit A.J. Hastie A.T. Edsall L.C. ...
Tópico(s): Lipid Membrane Structure and Behavior
2008 - Elsevier BV | Kidney International