Dr. Otto Dickel, Dr. L. Bordas, E. Wasmann, M. Gillmer, Dr. J. Dewitz, Dr. J. Hofer,
... Aberrationen Mimas Tiliae Ab. Tilioides, Holle (1865) Und Amorpha Populi Ab. Salicis Seu Palustris, Holle (1865).
0000 - Gale Group | NCCO-STM 1of2
Junli Han, Zhen Wang, Selvaraju Kanagarajan, Mengshu Hao, Anneli Lundgren, Peter E. Brodelius,
... 1A ). A key enzyme of this pathway is amorpha-4,11-diene synthase (ADS), which is the ... pathway converting farnesyldiphosphate (FDP) to the aliphatic sesquiterpene amorpha-4,11-diene. This intermediate has the right ... Brodelius P.E. Trichome-specific expression of the amorpha-4,11-diene 12-hydroxylase (cyp71av1) gene, encoding ... enzyme ADS-FDS will convert IDP + DMADP to amorpha-4,11-diene (shaded box in Figure 1A). ... A. Brodelius P.E. Trichome-specific expression of amorpha-4,11-diene synthase, a key enzyme of ...
Tópico(s): Insect and Pesticide Research
2016 - Elsevier BV | Molecular Plant
Xinzhu Sun, Songmiao Hu, Xin Wang, He Liu, Yun Zhou, Qingjie Guan,
Amorpha fruticosa L. is a deciduous shrub that is native to North America and has been introduced ... order to clarify the drought resistance characteristics of Amorpha fruticosa L. and excavate the related genes involved ... pathway, the mechanism of drought resistance stress of Amorpha fruticosa L. was revealed by the changes of transcriptome of Amorpha fruticosa L. under drought stress.Through the changes of the transcriptome of Amorpha fruticosa L. under drought stress, the mechanism of anti-stress of Amorpha fruticosa L. could be revealed.Different concentrations of ...
Tópico(s): Plant Molecular Biology Research
2021 - PeerJ, Inc. | PeerJ
Yansheng Zhang, Keat H. Teoh, Darwin W. Reed, Lies Maes, Alain Goossens, Douglas J. H. Olson, Andrew R. S. Ross, Patrick S. Covello,
... annua, the Δ11(13) double bond originating in amorpha-4,11-diene is reduced. This is thought ... annua, the Δ11(13) double bond originating in amorpha-4,11-diene is reduced. This is thought ... PubMed Scopus (188) Google Scholar). The formation of amorpha-4,11-diene by amorpha-4,11-diene synthase is the first committed ... is followed by oxidation at C-12 of amorpha-4,11-diene by the cytochrome P-450, ... EST, expressed sequence tag; AA, artemisinic acid; Ads, amorpha-4,11-diene synthase; AtOPR, A. thaliana 12- ... Cpr, A. annua cytochrome P-450 reductase; Cyp71av1, amorpha-4;11-diene monooxygenase; Dbr2, artemisinic aldehyde Δ11( ...
Tópico(s): Microbial Natural Products and Biosynthesis
2008 - Elsevier BV | Journal of Biological Chemistry
P. Mercke, Marie Bengtsson, Harro J. Bouwmeester, Maarten A. Posthumus, Peter E. Brodelius,
... isolated a cDNA clone encoding one of these, amorpha-4,11-diene synthase, a putative key enzyme ... the olefins as (E)-beta-farnesene (0.8%), amorpha-4,11diene (91.2%), amorpha-4,7(11)-diene (3.7%), gamma-humulene ( ... olefin (0.2%) and the oxygenated sesquiterpenes as amorpha-4-en-11-ol (0.2%) (tentatively), amorpha-4-en-7-ol (2.1%), and alpha- ... 0.3%) (tentatively). Using geranyl diphosphate as substrate, amorpha-4,11-diene synthase did not produce any ... pH 7.5. A putative reaction mechanism for amorpha-4,11-diene synthase is suggested.
Tópico(s): Essential Oils and Antimicrobial Activity
2000 - Elsevier BV | Archives of Biochemistry and Biophysics
Xiaoyan Qu, Y Diao, Z Zhang, Shaopeng Wang, Yuan Jia,
... the traditional Chinese medicine, the fresh fruits of Amorpha fruticosa L. were applied for the treatment of ... about the functional roles of the fruits of Amorpha fruticosa L. during wound healing progress. In the ... the seven compounds isolated from the fruits of Amorpha fruticosa L in vitro and in vivo. Our ... and VI (tephrosin) isolated from the fruits of Amorpha fruticosa L. performed dominant antimicrobial potential against microorganisms. ... could be possible for the therapeutic utilization of Amorpha fruticosa L. for wound healing in the future. ...
Tópico(s): Silk-based biomaterials and applications
2013 - African Traditional Herbal Medicine Supporters Initiative (ATHMSI) | African Journal of Traditional Complementary and Alternative Medicines
Harro J. Bouwmeester, T. Eelco Wallaart, Michiel H. A. Janssen, Bert van Loo, B. J. M. JANSEN, Maarten A. Posthumus, Claus O. Schmidt, Jan-Willem de Kraker, Wilfried Α. König, Maurice C. R. Franssen,
... detected a sesquiterpene with the mass spectrum of amorpha-4,11-diene. Synthesis of amorpha-4,11-diene from artemisinic acid confirmed the ... of the major activities catalysed the formation of amorpha-4,11-diene from farnesyl diphosphate. This enzyme ... 0.6 μM. The structure and configuration of amorpha-4,11-diene, its low content in A. annua and the high activity of amorpha-4,11-diene synthase all support that amorpha-4,11-diene is the likely olefinic sesquiterpene ...
Tópico(s): Pharmacological Effects of Natural Compounds
1999 - Elsevier BV | Phytochemistry
Junhao Shen, Haina Zheng, Xiaohui Zhi, Yanwei Shi, Yong Huang, Wei Wang, Yongqin Chen, Jian‐Qiang Kong, Ping Zhu,
Amorpha-4,11-diene is the precursor of the antimalarial compound artemisinin. The effect of Vitreoscilla hemoglobin (VHb) and its yeast-conform variant (VHbm) on amorpha-4,11-diene production in engineered Saccharomyces cerevisiae ... VHbm gene were constructed and introduced into the amorpha-4,11-diene-producing strain S. cerevisiae WK1 ... than VHb expression. GC-MS results indicated the amorpha-4,11-diene production in WK1[VHbm] and ... respectively. This suggests that VHb might improve the amorpha-4,11-diene production in engineered S. cerevisiae.
Tópico(s): Protein Structure and Dynamics
2012 - De Gruyter | Zeitschrift für Naturforschung C
Shannon C. K. Straub, Jeff J. Doyle,
Amorpha L. (false indigos and lead plants) is a North American legume genus of 16 species of ... and network analyses for evaluation of relationships amongst Amorpha species based on three non-coding plastome regions ( ... CNGC5, minD). Plastid DNA analyses supported a monophyletic Amorpha with Parryella filifolia and Errazurizia rotundata as successive ... of these two species and thus a paraphyletic Amorpha. Relationships among species of Amorpha were best resolved in the plastid DNA phylogeny ...
Tópico(s): Plant and animal studies
2014 - Elsevier BV | Molecular Phylogenetics and Evolution
Judit Kolozsváriné Nagy, Tímea Kiss, István Fehérváry, Csaba Vaszkó,
... 2) to evaluate the role of the invasive Amorpha fruticosa in increasing vegetation density, and (3) to ... 21 st century. Today forests are invaded by Amorpha fruticosa , which increases the vegetation density by 3% ... System) models, if floodplain vegetation was managed and Amorpha fruticosa was cleared from the floodplain, peak flood ... decreased by 4-6%, and the presence of Amorpha fruticosa reduced the flood flow velocities by 0. ... m/s. Accordingly, clearance of the floodplain from Amorpha fruticosa would have positive effects on flood protection, ...
Tópico(s): Flood Risk Assessment and Management
2018 - De Gruyter Open | Journal of Environmental Geography
Jinquan Huang, Dongmei Li, Xiu Tian, Jia‐Ling Lin, Lei Yang, Jing-Jing Xu, Xin Fang,
Amorpha-4,11-diene synthase (ADS) is the first committed enzyme in the biosynthesis of artemisinin. Artemisinin ... generate several minor products, including structural analogues of amorpha-4,11-diene, but their fate in fermentation ... found that ADS produces one of the analogues, amorpha-4-en-11-ol, as a pair of ... the side products of the recombinant ADS, including amorpha-4-en-7-ol, which is structurally similar to amorpha-4,11-diene. Our data uncover the complex ...
Tópico(s): Microbial Natural Products and Biosynthesis
2021 - American Chemical Society | Journal of Agricultural and Food Chemistry
Vitauts I. Kalnins, H. F. Stich, Sergio A. Bencosme,
... rings, consist of only few basic units: pars amorpha, nucleolonema, and Balbiani ring granules. The fine structure ... regions appears to be identical, consisting of pars amorpha, which contains fibers of varying diameters, and strands ... and ribosome-like granules. The arrangement of pars amorpha and nucleolonema of nucleoli and spherical micronucleoli follows ... pattern. Chromosome fibers are closely associated with pars amorpha, whereas strands of nucleolonema border only the surfaces of pars amorpha. Balbiani ring granules, which have a diameter of ...
Tópico(s): RNA Research and Splicing
1964 - NRC Research Press | Canadian Journal of Zoology
Hiroko Tsuruta, Christopher J. Paddon, Diana G. Eng, Jacob R. Lenihan, Tizita Horning, Larry C. Anthony, Rika Regentin, Jay D. Keasling, Neil S. Renninger, Jack D. Newman,
... source. Previous work showed that high levels of amorpha-4,11-diene, an artemisinin precursor, can be ... genes from Staphylococcus aureus, more than doubling production. Amorpha-4,11-diene titers were further increased by ... an average titer of 27.4 g/L amorpha-4,11-diene. Conclusions/ Significance Production of >25 g/L amorpha-4,11-diene by fermentation followed by chemical ...
Tópico(s): Pharmacological Effects of Natural Compounds
2009 - Public Library of Science | PLoS ONE
K. Jiang-Qiang, Wei Wang, W. Li-Na, Zhang Xiaodong, Cheng Kedi, Ping Zhu,
... variant of the Artemisia annua gene encoding for amorpha‐4,11‐diene synthase (ADS) on the production of amorpha‐4,11‐diene in a transformed yeast. Methods ... the transformed yeast cells produced higher level of amorpha‐4,11‐diene compared with the plant gene‐ ... were more efficient in terms of production of amorpha‐4,11‐diene than those transformed with the ...
Tópico(s): Fungal and yeast genetics research
2009 - Oxford University Press | Journal of Applied Microbiology
Jong‐Il Kim, Keon Heo, Yung‐Jin Chang, Si-Hyung Park, Sang‐Ki Rhee, Soo-Un Kim,
Cyclization of farnesyl diphosphate into amorpha-4,11-diene by amorpha-4,11-diene synthase (ADS) initiates biosynthesis of artemisinin, a clinically important antimalarial drug precursor. Three ... 1-2H2]farnesyl diphosphate to H-10 of amorpha-4,11-diene singled out the bisabolyl carbocation ... the 1S compound migrated to H-10 of amorpha-4,11-diene. Incorporation of one deuterium into ...
Tópico(s): Microbial Natural Products and Biosynthesis
2006 - American Chemical Society | Journal of Natural Products
Patrick J. Westfall, Douglas J. Pitera, Jacob R. Lenihan, Diana G. Eng, Frank X. Woolard, Rika Regentin, Tizita Horning, Hiroko Tsuruta, David J. Melis, Andrew E. Owens, Scott Fickes, Don Diola, Kirsten R. Benjamin, Jay D. Keasling, Michael D. Leavell, Derek McPhee, Neil S. Renninger, Jack D. Newman, Christopher J. Paddon,
... artemisinin based on production of the artemisinin precursor amorpha-4,11-diene by fermentation from engineered Saccharomyces ... the mevalonate pathway doubled artemisinic acid production, however, amorpha-4,11-diene production was 10-fold higher than artemisinic acid. We therefore focused on amorpha-4,11-diene production. Development of fermentation processes for the reengineered CEN.PK2 amorpha-4,11-diene strain led to production of > ... product. A chemical process was developed to convert amorpha-4,11-diene to dihydroartemisinic acid, which could ...
Tópico(s): Phytochemical compounds biological activities
2012 - National Academy of Sciences | Proceedings of the National Academy of Sciences
S. Picaud, P. Mercke, Xiaofei He, Olov Sterner, Maria Brodelius, David E. Cane, Peter E. Brodelius,
Recombinant amorpha-4,11-diene synthase from Artemisia annua, expressed in Escherichia coli, was incubated with the deuterium-labeled ... 1,1-2H2]FPP. GC–MS analysis of amorpha-4,11-diene formed from the deuterated FPPs ... of FPP is located at C-10 of amorpha-4,11-diene as a result of a ... afford the final product (1S,6R,7R,10R)-amorpha-4,11-diene.
Tópico(s): Microbial Natural Products and Biosynthesis
2005 - Elsevier BV | Archives of Biochemistry and Biophysics
S. Picaud, Linda Olofsson, Maria Brodelius, Peter E. Brodelius,
A cDNA clone encoding amorpha-4,11-diene synthase from Artemisia annua was subcloned into a bacterial expression vector in frame with a His6-tag. Recombinant amorpha-4,11-diene synthase was produced in Escherichia ... were analyzed by GC-MS. In addition to amorpha-4,11-diene, 15 sesquiterpenoids were produced. Only ... pH 6.5, 7.5, or 9.5. Amorpha-4,11-diene synthase showed significant increased product ...
Tópico(s): Microbial Natural Products and Biosynthesis
2005 - Elsevier BV | Archives of Biochemistry and Biophysics
Cinzia M. Bertea, J. Robert Freije, Hester van der Woude, F.W.A. Verstappen, L. Perk, V.E. Márquez, J-W. De Kraker, M. A. Posthumus, B. J. M. JANSEN, C.P.G.M. de Groot, Maurice C. R. Franssen, Harro J. Bouwmeester,
... L., particularly about the early enzymatic steps between amorpha-4,11-diene and dihydroartemisinic acid. Analyses of ... secretory cells revealed the presence of the oxygenated amorpha-4,11-diene derivatives artemisinic alcohol, dihydroartemisinic alcohol, ... a number of biosynthetic enzymes such as the amorpha-4,11-diene synthase and the--so far unknown--amorpha-4,11-diene hydroxylase as well as artemisinic ... that the early steps in artemisinin biosynthesis involve amorpha-4,11-diene hydroxylation to artemisinic alcohol, followed ...
Tópico(s): Malaria Research and Control
2005 - Thieme Medical Publishers (Germany) | Planta Medica
T. Eelco Wallaart, Harro J. Bouwmeester, Jacques Hille, L. Poppinga, Niels C. A. Maijers,
... into the first specific precursor of artemisinin, namely amorpha-4,11-diene. Here we describe the isolation of a cDNA clone encoding amorpha-4,11-diene synthase. The deduced amino acid ... coli, the recombinant enzyme catalyses the formation of amorpha-4,11-diene from farnesyl diphosphate. Introduction of ... of an active enzyme and the accumulation of amorpha-4,11-diene ranging from 0.2 to ...
Tópico(s): Pharmacological Effects of Natural Compounds
2001 - Springer Science+Business Media | Planta
Sun Young Choi, Hyun Jeong Lee, Jae‐Yeon Choi, Jiye Kim, Sang Jun Sim, Youngsoon Um, Yunje Kim, Taek Soon Lee, Jay D. Keasling, Han Min Woo,
... enzymes and the amorphadiene synthase for production of amorpha-4,11-diene, resulting in significantly increased levels (23-fold) of amorpha-4,11-diene (19.8 mg/L) in ... is the first demonstration of photosynthetic production of amorpha-4,11-diene from CO2 in cyanobacteria and ... or SQS demonstrated the highest production levels of amorpha-4,11-diene and squalene, which could expand ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2016 - Springer Science+Business Media | Biotechnology for Biofuels
... is usually referred to as nucleolonema and pars amorpha in animal nucleoli. In addition to nucleolonema and pars amorpha, two ultrastructurally and topographically distinct types of vacuole ... in diameter, and are confined to the pars amorpha of the nucleolus.
Tópico(s): Legume Nitrogen Fixing Symbiosis
1966 - Canadian Science Publishing | Canadian Journal of Botany
Stojanka Radulović, Dragana Skočajić, Ivana Bjedov, Danijela Djunisijević-Bojović,
Amorpha fruticosa is in many countries, and also in Serbia, one of the most dangerous invasive species. ... detection of mechanisms which inhibit the spreading of Amorpha population would be of great significance for finding ... all it is necessary, inter alia, to determine Amorpha ecological and coenological characteristics. The vegetation studies of ... Belgrade were compared. The study results indicate that Amorpha is present in forest communities, from the lowest ... are the shortest, or where they are absent. Amorpha forms thick brushland on meadows with different water ...
Tópico(s): Ecology and Vegetation Dynamics Studies
2008 - Faculty of Forestry, Belgrade | Glasnik Sumarskog fakulteta
T. W. J. M. van Herpen, Katarina Cankar, Marilise Nogueira, Dirk Bosch, Harro J. Bouwmeester, Jules Beekwilder,
... agro-infiltration. The first committed precursor of artemisinin, amorpha-4,11-diene, was produced upon infiltration of a construct containing amorpha-4,11-diene synthase, accompanied by 3-hydroxy-3-methylglutaryl-CoA reductase and farnesyl diphosphate synthase. Amorpha-4,11-diene was detected both in extracts ... co-infiltrated with the amorphadiene-synthesizing construct, the amorpha-4,11-diene levels strongly decreased, suggesting it ...
Tópico(s): Plant tissue culture and regeneration
2010 - Public Library of Science | PLoS ONE
Bing Wang, Sha Xue, Guo Bin Liu, Guanghui Zhang, Gary Li, Zong Ping Ren,
... Chinese pine, mixed trees of Chinese pine and amorpha, and mixed trees of black locust and amorpha. Soil properties on a sloped farmland, located in ... soil of mixed forest of black locust and amorpha increased significantly. However, nutrients content in the soil of mixed forest of Chinese pine and amorpha decreased. As to soil enzyme activities, saccharase, cellulase, ...
Tópico(s): Plant Ecology and Soil Science
2012 - Elsevier BV | CATENA
Jie Liu, En Tao Wang, Wen Xin Chen,
... the woody legumes Wisteria sinensis, Cercis racemosa and Amorpha fruticosa grown in the central and eastern regions ... of PCR–RFLP. Most of the isolates from Amorpha fruticosa were classified into two groups closely related ... grouping results was found in the isolates from Amorpha fruticosa. These results revealed that the symbiotic bacteria ...
Tópico(s): Coastal wetland ecosystem dynamics
2005 - Elsevier BV | Systematic and Applied Microbiology
Han Min Woo, Gregory Murray, Tanveer S. Batth, Nilu Prasad, Paul D. Adams, Jay D. Keasling, Christopher J. Petzold, Taek Soon Lee,
... pathway and examined their impact on production of amorpha-4,11-diene, an intermediate in the biosynthesis of the antimalarial drug artemisinin. Amorpha-4,11-diene titer increased almost three-fold ... and phosphomevalonate kinase significantly enhances the titer of amorpha-4,11-diene, and that further increases in ...
Tópico(s): Microbial Metabolic Engineering and Bioproduction
2013 - Elsevier BV | Chemical Engineering Science
... Dunav Str. 2, 1000 Sofia, Bulgaria Abstract:Background: Amorpha fruticosa L. and Phytolacca americana L. are native ... 2 mg/mL). Conclusions: Phytolacca americana leaves and Amorpha fruticosa could be useful in therapy of free radical pathologies and neurodegenerative disorders. Keywords:Acetylcholinesterase inhibition, Amorpha fruticosa, antioxidant activity, Phytolacca americanaView:PDF (793.82 ...
Tópico(s): Natural Antidiabetic Agents Studies
2013 - SAGE Publishing | Pharmacognosy Magazine
Yan Liu, Shi‐Hong Luo, Juan Hua, Desen Li, Yi Ling, Qian Luo, Sheng‐Hong Li,
... We identified six cadinene sesquiterpenes, including two volatiles (amorpha‐4,7(11)‐diene and (–)‐amorph‐4‐en‐ ... EaTPS1), catalyzing the conversion of farnesyl diphosphate to amorpha‐4,7(11)‐diene and (–)‐amorph‐4‐en‐ ... regulated EaTPS1 expression but caused the release of amorpha‐4,7(11)‐diene and (–)‐amorph‐4‐en‐ ... ol. Nicotiana benthamiana transiently expressing EaTPS1 also produced amorpha‐4,7(11)‐diene and (–)‐amorph‐4‐en‐ ...
Tópico(s): Plant Toxicity and Pharmacological Properties
2020 - Wiley | New Phytologist
Yiteng Zhang, Kai Wang, Zhenyu Wang, Xiufeng Li, Minghui Li, Fengjin Zhu, Zahid Majeed, Xingguo Lan, Qingjie Guan,
... through protoplast transformation. The expression of AfLOX4 in Amorpha fruticosa tissues was determined by quantitative real-time ... in the stems, roots, flowers, and leaves of Amorpha fruticosa L., and its expression level was the ... solid theoretical foundation for the genetic improvement of Amorpha fruticosa L. plant varieties, as well as improvement ...
Tópico(s): Insect-Plant Interactions and Control
2023 - Springer Science+Business Media | Acta Physiologiae Plantarum