Joana Liberal, Gustavo Costa, Anália do Carmo, Rui Vitorino, Carla Marques, M. Rosário M. Domingues, Pedro Domíngues, Ana Cristina Gonçalves, Raquel Alves, Ana Bela Sarmento‐Ribeiro, Henrique Girão, Maria Teresa Cruz, Maria Teresa Batista,
The hepatocellular carcinoma, a primary malignancy of the liver, has a very poor prognosis and a lower survival rate. Moreover, the inefficacy of conventional therapies emphasizes the importance of discovering new bioactive compounds. Several studies clearly state that plant-derived polyphenols, namely ellagitannins, have several health benefits. Fragaria vesca leaves contain high amounts of polyphenols, being especially rich in ellagitannins. Therefore, this study aimed to characterize an ellagitannin- ...
Tópico(s): Autophagy in Disease and Therapy
2015 - Elsevier BV | Arabian Journal of Chemistry
Mariana Freitas, Vera Alves, Ana Bela Sarmento‐Ribeiro, Anabela Mota‐Pinto,
Tópico(s): Boron Compounds in Chemistry
2011 - Elsevier BV | Biochemical and Biophysical Research Communications
Fernando Mendes, Cátia Domingues, Paulo Rodrigues‐Santos, Ana Margarida Abrantes, Ana Cristina Gonçalves, Jéssica Estrela, João Crispim Encarnação, Ana Salomé Pires, Mafalda Laranjo, Vera Alves, Ricardo Teixo, Ana Bela Sarmento‐Ribeiro, Maria Filomena Botelho, Manuel Santos Rosa,
Immune surveillance seems to represent an effective tumor suppressor mechanism. However, some cancer cells survive and become variants, being poorly immunogenic and able to enter a steady-state phase. These cells become functionally dormant or remain hidden clinically throughout. Neoplastic cells seem to be able to instruct immune cells to undergo changes promoting malignancy. Radiotherapy may act as a trigger of the immune response. After radiotherapy a sequence of reactions occurs, starting in ...
Tópico(s): Immune Cell Function and Interaction
2016 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer
A.C. Mamede, Sara Guerra, Mafalda Laranjo, Maria João Carvalho, Rui Caetano Oliveira, Ana Cristina Gonçalves, Raquel Alves, Lígia Castro, Ana Bela Sarmento‐Ribeiro, Paulo Moura, Ana Margarida Abrantes, Cláudio J. Maia, Maria Filomena Botelho,
Tópico(s): Proteoglycans and glycosaminoglycans research
2015 - Springer Science+Business Media | Medical Oncology
Mariana Freitas, Vera Alves, Ana Bela Sarmento‐Ribeiro, Anabela Mota‐Pinto,
Prostate cancer is the most common form of cancer affecting men in the Western world. Risk factors include ageing, genetics, recurrent inflammation, lifestyle and diet intake, related to an increase of oxidative stress. Prostate cancer risk is also associated with exposure to carcinogen such as polycyclic aromatic hydrocarbons (PAHs), originated from the incomplete combustion of carbon-containing fuels like tobacco, wood, diesel, or charbroiled meat. Although numerous studies have associated the ...
Tópico(s): Carcinogens and Genotoxicity Assessment
2013 - Scientific Research Publishing | Journal of Cancer Therapy
Ana Cristina Gonçalves, André Ribeiro, Vera Alves, Teresa Silva, Ana Bela Sarmento‐Ribeiro,
Tópico(s): Pesticide Exposure and Toxicity
2013 - Springer Science+Business Media | Biological Trace Element Research
Mariana Freitas, Inês Baldeiras, Teresa Proença, Vera Alves, Anabela Mota‐Pinto, Ana Bela Sarmento‐Ribeiro,
PC3 shows higher ROS production but also the highest GSH levels and Gl‐Red activity, possibly contributing to oxidative stress resistance. This is also associated with higher mitochondrial membrane potential, TAS and lower lipid peroxidation. On the other hand, we identified Gl‐Red activity reduction as a new strategy in overcoming oxidative stress resistance, by inducing H 2 O 2 cytotoxicity. Therefore these results suggest Gl‐Red activity reduction as a new potential therapeutic approach, in prostate ...
Tópico(s): Inflammatory mediators and NSAID effects
2012 - Wiley | FEBS Open Bio
Marı́lia Dourado, Ana Bela Sarmento‐Ribeiro, S. Pereira, Vera Alves, Henriqueta Coimbra Silva, Anabela Mota‐Pinto, Manuel Santos Rosa,
Dipeptidyl peptidase IV, a cell membrane surface protease also known as CD26 (CD26/DPPIV), is known to play multiple functions in human organism, where it is largely expressed, for instance, in the development of human cancer and metastasis as well as in chemotherapy response. The objective of this work was to study the CD26 membrane expression and DPPIV activity in T-acute leukaemia cell lines (CEM and MOLT3) in culture, in order to observe the modification of its expression under the 8-azaguanine ...
Tópico(s): Neuropeptides and Animal Physiology
2006 - Multidisciplinary Digital Publishing Institute | Pathophysiology
Carlos J.S. Alves, Ana Bela Cruzeiro,
We develop some numerical schemes for d -dimensional stochastic differential equations derived from Milstein approximations of diffusions which are obtained by lifting the solutions of the stochastic differential equations to higher dimensional spaces using geometrical tools, in the line of the work [A.B. Cruzeiro, P. Malliavin, A. Thalmaier, Geometrization of Monte-Carlo numerical analysis of an elliptic operator: Strong approximation, C. R. Acad. Sci. Paris, Ser. I 338 (2004) 481–486].
Tópico(s): Probabilistic and Robust Engineering Design
2007 - Elsevier BV | Stochastic Processes and their Applications
Maria Inês Costa, Beatriz Lapa, Joana Jorge, Raquel Alves, Isabel M. Carreira, Ana Bela Sarmento‐Ribeiro, Ana Cristina Gonçalves,
Genomic instability is prevented by the DNA damage response (DDR). Micronutrients, like zinc (Zn), are cofactors of DDR proteins, and micronutrient deficiencies have been related to increased cancer risk. Acute myeloid leukemia (AML) patients commonly present Zn deficiency. Moreover, reports point to DDR defects in AML. We studied the effects of Zn in DDR modulation in AML. Cell lines of AML (HEL) and normal human lymphocytes (IMC) were cultured in standard culture, Zn depletion, and supplementation ( ...
Tópico(s): Folate and B Vitamins Research
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Joana Jorge, Joana Neves, Raquel Alves, Catarina Geraldes, Ana Cristina Gonçalves, Ana Bela Sarmento‐Ribeiro,
Lymphoid malignancies are a group of highly heterogeneous diseases frequently associated with constitutive activation of the nuclear factor kappa B (NF-κB) signaling pathway. Parthenolide is a natural compound used to treat migraines and arthritis and found to act as a potent NF-κB signaling inhibitor. This study evaluated in vitro parthenolide efficacy in lymphoid neoplasms. We assessed parthenolide metabolic activity in NCI-H929 (MM), Farage (GCB-DLBCL), Raji (BL), 697 and KOPN-8 (B-ALL), and CEM and ...
Tópico(s): Natural product bioactivities and synthesis
2023 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Produção Nacional
Thádia Evelyn de Araújo, Jordana Grazziela Alves Coelho-dos-Reis, Samantha Ribeiro Béla, Ana Carolina Aguiar Vasconcelos Carneiro, Anderson Silva Machado, Ludmila Melo Cardoso, Ágata Lopes Ribeiro, Michelle Hallais França Dias, Gláucia Manzan Queiroz de Andrade, Ricardo Wagner Almeida Vítor, José Nelio Januário, Andréa Teixeira‐Carvalho, Ricardo Wagner Almeida Vítor, Eloísa Amália Vieira Ferro, Olindo Assis Martins‐Filho,
The present study characterized the early changes in the serum chemokines/cytokine signatures and networks in infants with congenital-toxoplasmosis/(TOXO) as compared to non-infected-controls/(NI). TOXO were subgrouped according to the retinochoroidal lesion status as no-lesion/(NL), active-lesion/(ARL), active/cicatricial-lesion/(ACRL) and cicatricial-lesion/(CRL). The results showed that TOXO display prominent chemokine production mediated by IL-8/CXCL8, MIG/CXCL9, IP-10/CXCL10 and RANTES/CCL5. ...
Tópico(s): interferon and immune responses
2017 - Elsevier BV | Cytokine
Fernando Ribeiro, Ilda Patrícia Ribeiro, Ana Cristina Gonçalves, Alberto J. Alves, Elsa Melo, Raquel Fernandes, Rui Costa, Ana Bela Sarmento‐Ribeiro, José Alberto Duarte, Isabel M. Carreira, Sarah Witkowski, José Oliveira,
This study aimed to determine the effect of a single bout of resistance exercise at different intensities on the mobilization of circulating EPCs over 24 hours in women. In addition, the angiogenic factors stromal cell-derived factor 1 (SDF-1α), vascular endothelial growth factor (VEGF), hypoxia-inducible factor 1-alpha (HIF-1α) and erythropoietin (EPO) were measured as potential mechanisms for exercise-induced EPCs mobilization. Thirty-eight women performed a resistance exercise session at an intensity ...
Tópico(s): Lymphatic System and Diseases
2017 - Nature Portfolio | Scientific Reports
Raquel Alves, Ana Cristina Gonçalves, Joana Jorge, António Almeida, Ana Bela Sarmento‐Ribeiro,
Multidrug resistance (MDR) development has emerged as a complication that compromises the success of several chemotherapeutic agents. In chronic myeloid leukemia (CML), imatinib resistance has been associated with changes in BCR-ABL1 and intracellular drug concentration, controlled by SLC and ABC transporters. We evaluate the therapeutic potential of a P-glycoprotein and BCRP inhibitor, elacridar, in sensitive (K562 and LAMA-84) and imatinib-resistant (K562-RC and K562-RD) CML cell lines as monotherapy ...
Tópico(s): HIV/AIDS drug development and treatment
2022 - Multidisciplinary Digital Publishing Institute | Biomedicines
Joana Jorge, Sara Petronilho, Raquel Alves, Margarida Coucelo, Ana Cristina Gonçalves, Nascimento Costa, Ana Bela Sarmento‐Ribeiro,
Tópico(s): Cancer-related gene regulation
2019 - Springer Science+Business Media | Investigational New Drugs
Ana Cristina Gonçalves, Vera Alves, Teresa Silva, Cristina Carvalho, Catarina R. Oliveira, Ana Bela Sarmento‐Ribeiro,
The Myelodysplastic Syndromes are stem cell heterogeneous disorders characterized by peripheral cytopenias and hypercellular bone marrow, which can evolute to acute leukaemia. Vitamin C can act as an antioxidant, ascorbic acid (AA) donates two electrons and becomes oxidized to dehydroascorbic acid (DHA). Under physiological conditions, vitamin C predominantly exists in its reduced (AA) form but also exists in trace quantities in the oxidized form (DHA). This study evaluates the therapeutic potential ...
Tópico(s): Retinoids in leukemia and cellular processes
2013 - Elsevier BV | Toxicology in Vitro
Produção Nacional
Revisado por pares
Paula Monalisa Nogueira, Kleber Silva Ribeiro, Amanda C. O. Silveira, João H. C. Campos, Olindo Assis Martins‐Filho, Samantha Ribeiro Béla, Marco Antônio Campos, Natália Lima Pessoa, Walter Colli, Maria Júlia Manso Alves, Rodrigo Pedro Soares, Ana Cláudia Torrecilhas,
Trypomastigote forms of Trypanosoma cruzi, the causative agent of Chagas Disease, shed extracellular vesicles (EVs) enriched with glycoproteins of the gp85/trans-sialidase (TS) superfamily and other α-galactosyl (α-Gal)-containing glycoconjugates, such as mucins. Here, purified vesicles from T. cruzi strains (Y, Colombiana, CL-14 and YuYu) were quantified according to size, intensity and concentration. Qualitative analysis revealed differences in their protein and α-galactosyl contents. Later, those ...
Tópico(s): Parasites and Host Interactions
2015 - Taylor & Francis | Journal of Extracellular Vesicles
José Eduardo Mendes, Ana Cristina Gonçalves, Raquel Alves, Joana Jorge, Ana Salomé Pires, Ana Bela Sarmento‐Ribeiro, Ana Bela Sarmento‐Ribeiro,
Non-Hodgkin Lymphoma (NHL) constitutes a very heterogeneous group of diseases with different aggressiveness. Diffuse large B-cell lymphoma (DLBCL) and Burkitt's lymphoma (BL) are two clinically aggressive lymphomas from the germinal center, very heterogeneous and with different genetic signatures. Several intracellular pathways are involved in lymphomagenesis, being BCR/PI3K/AKT/mTOR and RAS/RAF pathways the most frequently ones. In this context the therapeutic potential of a mTOR inhibitor – everolimus – ...
Tópico(s): PI3K/AKT/mTOR signaling in cancer
2015 - Springer Science+Business Media | Pathology & Oncology Research
Ana Cristina Gonçalves, Emília Cortesão, Bárbara Oliveiros, Vera Alves, Ana Espadana, Luís Rito, Emília Magalhães, Sónia Alves Pereira, Amélia Pereira, Nascimento Costa, Luísa Mota‐Vieira, Ana Bela Sarmento‐Ribeiro,
Tópico(s): Glutathione Transferases and Polymorphisms
2015 - Springer Science+Business Media | Clinical and Experimental Medicine
Raquel Alves, Ana Cristina Gonçalves, Joana Jorge, Joana Alves, António Alves da Silva, Paulo Freitas-Tavares, Nascimento Costa, António Almeida, Ana Bela Sarmento‐Ribeiro,
Tópico(s): PI3K/AKT/mTOR signaling in cancer
2019 - Springer Science+Business Media | Medical Oncology
Produção Nacional
Revisado por pares
Aline de Castro Zacche-Tonini, Giuliana Schmidt França Fonseca, Laura Néspoli Nassar Pansini de Jesus, Geisa Baptista Barros, Jordana Grazziela Alves Coelho-dos-Reis, Samantha Ribeiro Béla, Anderson Silva Machado, Ana Carolina Aguiar Vasconcelos Carneiro, Gláucia Manzan Queiroz de Andrade, Ricardo Wagner Almeida Vítor, José Nelio Januário, Andréa Teixeira‐Carvalho, Ricardo Wagner Almeida Vítor, Eloísa Amália Vieira Ferro, José Roberto Mineo, Olindo Assis Martins‐Filho, Elenice Moreira Lemos,
The aim of this study was to evaluate the performance of conventional serology (Q-Preven™ and ELFAVIDAS™) and flow cytometry-based serologic tools for early serologic diagnosis of congenital toxoplasmosis. The study groups included prospectively confirmed cases of congenital toxoplasmosis (TOXO=88) and age-matching non-infected controls (NI=15).The results demonstrated that all samples tested positive/indeterminate for anti-T. gondii IgM screening at birth using air-dried whole blood samples. Serum ...
Tópico(s): Herpesvirus Infections and Treatments
2017 - Elsevier BV | Journal of Immunological Methods
Joana Jorge, Nisa Magalhães, Raquel Alves, Beatriz Lapa, Ana Cristina Gonçalves, Ana Bela Sarmento‐Ribeiro,
Acute lymphoblastic leukemia (ALL) is one of the most common hematological malignancies at pediatric ages and is characterized by different chromosomal rearrangements and genetic abnormalities involved in the differentiation and proliferation of lymphoid precursor cells. Brusatol is a quassinoid plant extract extensively studied due to its antineoplastic effect through global protein synthesis and nuclear factor erythroid 2-related factor-2 (NRF2) signaling inhibition. NRF2 is the main regulator of ...
Tópico(s): Genomics, phytochemicals, and oxidative stress
2022 - Multidisciplinary Digital Publishing Institute | Biomedicines
Raquel Alves, Ana M. Figueiredo, Ana Cristina Gonçalves, Margarida Ferreira-Teixeira, Joana Esteves de Lima, Ana Margarida Abrantes, Vera Alves, Paulo Rodrigues‐Santos, Lénia Jorge, Eunice Matoso, Isabel M. Carreira, Maria Filomena Botelho, Ana Bela Sarmento‐Ribeiro,
Imatinib resistance has been associated with BCR-ABL alterations, but other mechanisms might be involved, like drug transporters. Additionally, the impact of poor adherence in resistance has been little explored. Using sensitive and resistance CML cell lines, we investigated the expression of influx/efflux transporters, like P-gP and OCT1. In the therapeutic interruption model, we observed decrease of influx and increase in efflux transporters combined with BCR-ABL over-expression. Comparatively, ...
Tópico(s): Mast cells and histamine
2014 - Elsevier BV | Leukemia Research
Raquel Alves, Ana Cristina Gonçalves, Sergio Rutella, António Almeida, Javier De Las Rivas, Ioannis P. Trougakos, Ana Bela Sarmento‐Ribeiro,
Resistance to targeted therapies is a complex and multifactorial process that culminates in the selection of a cancer clone with the ability to evade treatment. Chronic myeloid leukemia (CML) was the first malignancy recognized to be associated with a genetic alteration, the t(9;22)(q34;q11). This translocation originates the BCR-ABL1 fusion gene, encoding the cytoplasmic chimeric BCR-ABL1 protein that displays an abnormally high tyrosine kinase activity. Although the vast majority of patients with ...
Tópico(s): Myeloproliferative Neoplasms: Diagnosis and Treatment
2021 - Multidisciplinary Digital Publishing Institute | Cancers
Raquel Alves, Stéphanie McArdle, Jayakumar Vadakekolathu, Ana Cristina Gonçalves, Paulo Freitas-Tavares, Amélia Pereira, António Almeida, Ana Bela Sarmento‐Ribeiro, Sergio Rutella,
Abstract Background Tumor cells have evolved complex strategies to escape immune surveillance, a process which involves NK cells and T lymphocytes, and various immunological factors. Indeed, tumor cells recruit immunosuppressive cells [including regulatory T-cells (Treg), myeloid-derived suppressor cells (MDSC)] and express factors such as PD-L1. Molecularly targeted therapies, such as imatinib, have off-target effects that may influence immune function. Imatinib has been shown to modulate multiple ...
Tópico(s): Acute Myeloid Leukemia Research
2020 - BioMed Central | Journal of Translational Medicine
Ana Margarida Paiva, Bela Barros, Marta Oliveira, Sara Alves, Filipa Esteves, Adília Fernandes, Josiana A. Vaz, Klára Slezáková, João Paulo Teixeira, Solange Costa, Simone Morais,
Human biomonitoring data retrieved from real-life wildland firefighting in Europe and, also, worldwide are scarce. Thus, in this study, 176 Portuguese firefighters were biomonitored pre- and post- unsimulated wildfire combating (average:12–13 h; maximum: 55 h) to evaluate the impact on the levels of urinary polycyclic aromatic hydrocarbons hydroxylated metabolites (OHPAH; quantified by high-performance liquid chromatography with fluorescence detection) and the associated short-term health effects ( ...
Tópico(s): Injury Epidemiology and Prevention
2024 - Elsevier BV | The Science of The Total Environment
Beatriz Lapa, Ana Cristina Gonçalves, Joana Jorge, Raquel Alves, Ana Salomé Pires, Ana Margarida Abrantes, Margarida Coucelo, Antero Abrunhosa, Maria Filomena Botelho, Nascimento Costa, Ana Bela Sarmento‐Ribeiro,
Tópico(s): Epigenetics and DNA Methylation
2020 - Springer Science+Business Media | Medical Oncology
Ana Cristina Gonçalves, Emília Cortesão, Bárbara Oliveiros, Vera Alves, Ana Espadana, Luís Rito, Eunice Magalhães, Maria João Lobão, Amélia Pereira, Nascimento Costa, Luísa Mota‐Vieira, Ana Bela Sarmento‐Ribeiro,
The imbalance between reactive oxygen species (ROS) production and their elimination by antioxidants leads to oxidative stress. Depending on their concentration, ROS can trigger apoptosis or stimulate cell proliferation. We hypothesized that oxidative stress and mitochondrial dysfunction may participate not only in apoptosis detected in some myelodysplastic syndrome (MDS) patients, but also in increasing proliferation in other patients. We investigated the involvement of oxidative stress and mitochondrial ...
Tópico(s): Neutrophil, Myeloperoxidase and Oxidative Mechanisms
2015 - Taylor & Francis | Free Radical Research
Produção Nacional
Revisado por pares
Anderson Silva Machado, Ana Carolina Aguiar Vasconcelos Carneiro, Samantha Ribeiro Béla, Gláucia Manzan Queiroz de Andrade, Ricardo Wagner Almeida Vítor, José Nelio Januário, Jordana Grazziela Alves Coelho-dos-Reis, Eloísa Amália Vieira Ferro, Andréa Teixeira‐Carvalho, Ricardo Wagner Almeida Vítor, Olindo Assis Martins‐Filho, UFMG Congenital Toxoplasmosis Group Brazilian —UFMG-CTBG,
Toxoplasma gondii is the main infectious cause of human posterior retinochoroiditis, the most frequent clinical manifestation of congenital toxoplasmosis. This investigation was performed after neonatal screening to identify biomarkers of immunity associated with immunopathological features of the disease by flow cytometry. The study included infected infants without NRL and with retinochoroidal lesions (ARL, ACRL, and CRL) as well as noninfected individuals (NI). Our data demonstrated that leukocytosis, ...
Tópico(s): Herpesvirus Infections and Treatments
2014 - Hindawi Publishing Corporation | Mediators of Inflammation
S. G. Sousa, Vasco Pinto, Filipe Vaz da Silva, Tiago Ribeiro da Costa, Armindo Fernandes, Rodrigo Batata, Carolina Noronha, João Silva, Sónia Ferreira, Salomé Sobral Sousa, Célia Alves, Rui Rangel, Alfredo Calheiros, _ _, Jorge M. Antunes, Miguel Fidalgo, Ana Grande, Gonçalo Figueiredo, L.A. Rocha, Eduardo Grala da Cunha, Miguel A. Ferreira, Sérgio Rodrigues Moreira, Ana Lúcia Machado, Márcia Tizziani, Carla Silva, Elsa Silva, Joaquim Reis, Mário Gomes, Célia Pinheiro, Dora Simões, Isabel Ribeiro, Pedro Amorim, Sara Barbeiro, Vanessa Teixeira, Sílvia Helena Henriques Camelo, Maria Laura Gonçalves, Graça Maria Alves dos Santos Magalhães, Ana Cristina Rodrigues Lacerda, Ana Paula Couto, Ana Paula Silva, Ana Bela Sarmento‐Ribeiro, Ana Rita Poças, Anabela Neves, Andreia Portela, Andreia Preto, Andreia Queirós, António de Castro Caeiro, Ariana Rocha, Bruna Mateus, Cristiana Pereira, Daniel Ferreira da Cunha, Daniela M. Sousa, Elsa Oliveira, Ema Paula Ribeiro, Hélder R. O. Rocha, Inês A. Barbosa, Inês Carvalho, Inês Cunha, Inês Lima, Inês Falcão‐Pires, Inês Maria Meneses dos Santos, Isabel Pavão Martins, Isabel Ramos, Joana Silva, Jorge Castanheira, J.A.M. Ferreira, Júlia Leitão, Juliana Silva, Leonela Margarita Torre, Lucília Alves, Vasco Pinto, Margarida Chagas Lopes, Maria do Céu Amaral, Maria João Rocha Melo, Maria Monteiro, Maria Teresa Lisboa, Mariana Silva, Marília Saffarizadeh, Marisa Moreira, Marlene Luz, Noémia Costa, Patrícia Capas, Rita Quintela, Rosa Silva, Rui Neto, Rui Santos, Sílvia Silva, Simão Pinto, Sofia Queirós, Tiago Oliveira,
Timing of mobilization after chronic subdural hematoma (cSDH) surgery is highly heterogeneous among neurosurgical centers. Past studies have suggested that early mobilization may reduce medical complications without increasing recurrence, but evidence remains scarce. The purpose of this study was to compare an early mobilization protocol with a 48-hour bed rest practice, with a focus on the occurrence of medical complications.The GET-UP Trial is a prospective, randomized, unicentric, open-label study ...
Tópico(s): Pituitary Gland Disorders and Treatments
2023 - American Association of Neurological Surgeons | Journal of neurosurgery