Emiko Isogai, Chitwambi Makungu, John Yabe, Patson Sinkala, Andrew Nambota, Hiroshi Isogai, Hideto Fukushi, Manda Silungwe, Charles Mubita, Michelo Syakalima, Bernard M. Hang’ombe, Shunji Kozaki, Jun Yasuda,
The isothermal and chimeric primer-initiated amplification of nucleic acids (ICAN) is a new isothermal DNA amplification method composed of exo Bca DNA polymerase, RNaseH and DNA-RNA chimeric primers. We detected invA of Salmonella from chicken carcasses, egg yolk and cattle fecal samples. Fifty-three of 59 isolates were invA-positive in ICAN-chromatostrip detection. The result was consistent with those obtained by standard PCR. Salmonella invA was detected in 12 of 14 carcass rinses by ICAN, while ...
Tópico(s): Identification and Quantification in Food
2005 - Elsevier BV | Comparative Immunology Microbiology and Infectious Diseases
Victor Owino, Beatrice Amadi, Moses Sinkala, Suzanne Filteau, Andrew Tomkins,
Poor quality complementary foods with low nutrient density and inappropriate feeding practices have been identified among the major causes of malnutrition in young children. In many developing countries, complementary foods are introduced too early or too late and the quality and quantity of the foods are insufficient, leading to a great risk of nutritional deficiencies during the second half of infancy. Most of the habitually used complementary foods in developing countries are unfortified cerealbased ...
Tópico(s): Child Nutrition and Feeding Issues
2008 - African Scholarly Science Communications Trust | African Journal of Food Agriculture Nutrition and Development
Mpundu Makasa, Lackson Kasonka, Molly Chisenga, Moses Sinkala, Chifumbe Chintu, Andrew Tomkins, Suzanne Filteau,
Summary Objective Parental HIV infection may affect even those exposed children who remain uninfected. We investigated early growth, an indicator of overall health, of infants born to Zambian mothers recruited for a study of breastfeeding and postpartum health. Methods HIV‐infected and uninfected women in Lusaka were followed regularly from late pregnancy to 16 weeks postpartum. Infant weight and length were measured at birth, 6 and 16 weeks. Infant HIV status could not be specifically determined ...
Tópico(s): HIV Research and Treatment
2007 - Wiley | Tropical Medicine & International Health
Victor Owino, Moses Sinkala, Beatrice Amadi, Andrew Tomkins, Suzanne Filteau,
Abstract The effects of α‐amylase treatment on physical properties, acceptability to mothers, and cost of roasted and extruded maize–beans–groundnuts–bambaranuts complementary porridge recipes were assessed prior to their industrial production. Storage stability of the extruded α‐amylase‐treated fortified blend was assessed at 2 weeks and 6 months by sensory evaluation, peroxide value, water activity and microbiological load. The use of α‐amylase at 0.04% w/w enhanced porridge acceptability and ...
Tópico(s): Child Nutrition and Water Access
2007 - Wiley | Journal of the Science of Food and Agriculture
Rachel Gitau, Mpundu Makasa, Lackson Kasonka, Moses Sinkala, Chifumbe Chintu, Andrew Tomkins, Suzanne Filteau,
Abstract Objective To investigate the effects on maternal micronutrient status and infant growth of the increased maize prices that resulted from the southern African drought of 2001–2002. Design Longitudinal cohort study. Setting A maternal and child health clinic in Lusaka, Zambia. Subjects Maternal and infant health and nutrition data and maternal plasma were being collected for a study of breast-feeding and postpartum health. Samples and data were analysed according to whether they were collected ...
Tópico(s): Food Security and Health in Diverse Populations
2005 - Cambridge University Press | Public Health Nutrition
Edgar Simulundu, Yona Sinkala, Herman M. Chambaro, Andrew Chinyemba, Frank Banda, Lynnfield E. Mooya, Joseph Ndebe, Simbarashe Chitanga, Chitwambi Makungu, Gift Munthali, Paul Fandamu, Ayato Takada, Aaron S. Mweene,
African swine fever (ASF) is a contagious haemorrhagic disease associated with causing heavy economic losses to the swine industry in many African countries. In 2017, Zambia experienced ASF outbreaks in Mbala District (Northern province) and for the first time in Isoka and Chinsali districts (Muchinga province). Meanwhile, another outbreak was observed in Chipata District (Eastern province). Genetic analysis of part of the B646L gene, E183L gene, CP204L gene and the central variable region of the B602L ...
Tópico(s): Viral gastroenteritis research and epidemiology
2018 - AOSIS | Onderstepoort Journal of Veterinary Research
William Phiri, Lackson Kasonka, Simon M. Collin, Mpundu Makasa, Moses Sinkala, Chifumbe Chintu, Francis Kasolo, Christine Kaseba, Andrew Tomkins, Suzanne Filteau,
In a longitudinal cohort study we investigated factors contributing to breast milk HIV RNA viral load among lactating women in Lusaka, Zambia. Detailed data from 135 HIV-infected women were collected by questionnaires concerning postpartum maternal and infant health and infant feeding practice. Maternal blood was collected during pregnancy and at 6 weeks postpartum. Milk samples collected from each breast at 10 days and 6 weeks postpartum plus a subset collected at other time points were analyzed ...
Tópico(s): HIV-related health complications and treatments
2006 - Mary Ann Liebert, Inc. | AIDS Research and Human Retroviruses
Molly Chisenga, Lackson Kasonka, Mpundu Makasa, Moses Sinkala, Chifumbe Chintu, Christine Kaseba, Francis Kasolo, Andrew Tomkins, Susan F. Murray, Suzanne Filteau,
Exclusive breastfeeding (EBF) is optimal for infant health and is associated with decreased risk of mother-to-child HIV transmission compared with mixed feeding of breast milk and other foods. To investigate why many women stop EBF before the recommended 6 months, maternal and infant health and infant-feeding data were collected from 177 HIV-infected and 177-uninfected Zambian women regularly from 34 weeks gestation to 16 weeks postpartum. Despite strong support for good breastfeeding practice, only ...
Tópico(s): HIV-related health complications and treatments
2005 - SAGE Publishing | Journal of Human Lactation
Lackson Kasonka, Mpundu Makasa, Tom Marshall, Molly Chisenga, Moses Sinkala, Chifumbe Chintu, Christine Kaseba, Francis Kasolo, Rachel Gitau, Andrew Tomkins, Susan F. Murray, Suzanne Filteau,
Summary Subclinical mastitis, defined as raised milk sodium/potassium (Na/K) ratio, is associated with poor infant growth and, among HIV‐infected women, with increased milk HIV viral load. We conducted a longitudinal cohort study in Lusaka, Zambia, in order to investigate the relative importance of several potential causes of subclinical mastitis: maternal infection, micronutrient deficiencies and poor lactation practice. Women (198 HIV‐infected, 189 HIV‐uninfected) were recruited at 34 weeks’ gestation ...
Tópico(s): HIV/AIDS Research and Interventions
2006 - Wiley | Paediatric and Perinatal Epidemiology
Victor Owino, Lackson Kasonka, Moses Sinkala, Jonathan K Wells, Simon Eaton, Tegan Darch, A. Coward, Andrew Tomkins, Suzanne Filteau,
Background: Malnutrition in late infancy in developing countries may result from poor-quality complementary foods that displace breast milk. Objective: The objective of the study was to assess the effects of fortified complementary blends of different energy densities on growth, hemoglobin concentrations, and breast milk intake of 9-mo-old Zambian infants. Design: Infants were randomly assigned at 6 mo of age to receive for 3 mo a fortified blend of maize, beans, bambaranuts, and groundnuts [Chilenje ...
Tópico(s): Breastfeeding Practices and Influences
2007 - Elsevier BV | American Journal of Clinical Nutrition
Edgar Simulundu, Kunda Ndashe, Herman M. Chambaro, David Squarre, Paul Michael Reilly, Simbarashe Chitanga, Katendi Changula, Andrew Nalishuwa Mukubesa, Joseph Ndebe, John Tembo, Nathan Kapata, Matthew Bates, Yona Sinkala, Bernard M. Hang’ombe, King S. Nalubamba, Masahiro Kajihara, Michihito Sasaki, Yasuko Orba, Ayato Takada, Hirofumi Sawa,
We detected West Nile virus (WNV) nucleic acid in crocodiles (Crocodylus niloticus) in Zambia. Phylogenetically, the virus belonged to lineage 1a, which is predominant in the Northern Hemisphere. These data provide evidence that WNV is circulating in crocodiles in Africa and increases the risk for animal and human transmission.
Tópico(s): COVID-19 epidemiological studies
2020 - Centers for Disease Control and Prevention | Emerging infectious diseases
Herman M. Chambaro, Michihito Sasaki, Edgar Simulundu, Isaac Silwamba, Yona Sinkala, Gabriel González, David Squarre, Paul Fandamu, Caesar H. Lubaba, Musso Munyeme, Alikhadio Maseko, Choopa Chimvwele, Liywalii Mataa, Lynnfield E. Mooya, Andrew Nalishuwa Mukubesa, Hayato Harima, K.L. Samui, Hetron Mweemba Munang’andu, Martin Simuunza, King S. Nalubamba, Yongjin Qiu, Michael J. Carr, William W. Hall, Yuki Eshita, Hirofumi Sawa, Yasuko Orba,
Bluetongue (BT) is an arthropod-borne viral disease of ruminants with serious trade and socio-economic implications. Although the disease has been reported in a number of countries in sub-Saharan Africa, there is currently no information on circulating serotypes and disease distribution in Zambia. Following surveillance for BT in domestic and wild ruminants in Zambia, BT virus (BTV) nucleic acid and antibodies were detected in eight of the 10 provinces of the country. About 40% (87/215) of pooled blood ...
Tópico(s): Viral Infections and Vectors
2020 - Multidisciplinary Digital Publishing Institute | Viruses
Rachel Gitau, Lackson Kasonka, K. J. Newens, Molly Chisenga, Mpundu Makasa, Francis Kasolo, Moses Sinkala, Chifumbe Chintu, Christine Kaseba, Andrew Tomkins, Suzanne Filteau,
... Oxford Academic PubMed Google Scholar Moses Sinkala, Moses Sinkala Search for other works by this author on: Oxford Academic PubMed Google Scholar Chifumbe Chintu, Chifumbe Chintu Search for other works by this author on: Oxford Academic PubMed Google Scholar Christine Kaseba, Christine Kaseba Search for other works by this author on: Oxford Academic PubMed Google Scholar Andrew Tomkins, Andrew Tomkins Search for other works by ...
Tópico(s): HIV-related health complications and treatments
2003 - Oxford University Press | Transactions of the Royal Society of Tropical Medicine and Hygiene
Andrew J. Prendergast, Mark F. Cotton, Diana M. Gibb,
... Chi BH, Chintu N, Lee A, Stringer EM, Sinkala M, Stringer JS: Expanded services for the prevention ...
Tópico(s): HIV-related health complications and treatments
2008 - Future Medicine | Future HIV Therapy
Myrna Tonkinson, Peter Sutton, Peggy Brock, Andrew Markus, Davids Trigger, David Mardiros, Peter Jull, Ian H. Frazer, Michael Howard, Margaret Critchlow Rodman, Don Gardner, Caroline Ralston, R.J. May, Michael Aung‐Thwin, K. Richard Young, Michael L. Roberts, Jay H. Bernstein, Athol Chase, Basil Sansom, Helen Leach, Richard Riordan,
... 4250 (hardback). ISBN 0–8047–1666–8. A Sinkala Village in a Time of Trouble: Politics and ...
Tópico(s): Rangeland Management and Livestock Ecology
1993 - Taylor & Francis | Anthropological Forum
Ian H. Thorneycroft, R. Lindsay, James H. Pickar, Jennifer A. Doherty, Kara L. Cushing‐Haugen, Babette S. Saltzman, Lynda F. Voigt, Deirdre A. Hill, Shirley A.A. Beresford, Chu Chen, Noel S. Weiss, Kathleen N. Moore, Amanda Cofer, Leslie Elliot, Grainger S. Lanneau, Joan L. Walker, Michael S. Gold, Elizabeth Stringer, Christine Kaseba, Jens Levy, Moses Sinkala, Robert L. Goldenberg, Benjamin H. Chi, Inutu Matongo, Sten, H Vermund, Mulindi Mwanahamuntu, Jeffrey S. A. Stringer, Rachna Bahl, Roshni Patel, Rebecca Swingler, Matthew Ellis, Deirdre J. Murphy, Steven R. Nesheim, Denise J. Jamieson, Susan P. Danner, Robert Maupin, Lisanne Palomar, Emily DeFranco, Kirstin Lee, Jenifer E. Allsworth, Louis J. Muglia, Christian Briery, Carl H. Rose, William Henry Hudson, Monica A. Lutgendorf, Everett F. Magann, Suneet P. Chauhan, John C. Morrison, Joanna F. Crofts, Denise Ellis, Mark James, Linda Hunt, Robert, Jiri Sonek, M. Borenstein, Cathy Downing, David S. McKenna, Ran Neiger, Christopher S. Croom, Toby Genrich, Kypros Nicolaides Frontomaxillary, Jun Zhang, José Villar, Wenyu Sun, Mario Merialdi, Hany, Matthews Mathai, Mohamed Ashraf Ali, Kai Yu, Nelly Zavaleta, Manorama Purwar, Nguyen, Thi Nhu, Liana Campodónico, Sihem Landoulsi, Marshall D. Lindheimer, Guillermo Carroli, Thomas J. Garite, Moon Kim, Edward J. Quilligan, Frederick P. Zuspan, Richard C. Bump, Sandra Ann Carson, Philip Saia, Steven G. Gabbe, Jay D. Iams, Sarah J. Kilpatrick, George A. Macones, Roberto Romero, Larry Sachs, Mary D. Sammel, Sandra Perrine, Marcia Ringel, Lee B. Epstein, Carol Graham, Michael Heit, Mary Townsend, Kim N. Danforth, Karen L. Lifford, Bernard Rosner, Gary Curhan, Neil M. Resnick, Francine Grodstein, Jennifer Patterson, Philippe H. Girerd, Nicole W. Karjane, Kimberly K. Jefferson, Tracy Behm, James B. Unger, Joseph J. Ivy, Debi P. Mukherjee, Christopher Robinson, Donna Johnson, Anne C Staff, Kristin Brække, Guro M. Johnsen, Sudha Ananth, Nina Kittelsen Harsem, Erica Harnett, Michelle Dickinson, Graeme Smith, Ray Bahado‐Singh, Judith Balk, Sarah L. Berga, Michael J. Birrer, Wendy R. Brewster, William Camann, Frank A. Chervenak, Ethics Clark, Arnold W. Cohen, Murray Freedman, Angela Gantt, Robert E. Garfield, Ronald S. Gibbs, Linda C. Giudice, Steven Goldstein, Susan Hendrix, Willie Andersen, Ann Hatfield, Luis Sanchez‐Ramos, Andrew M. Kaunitz,
Additional evidence suggests that the use of continuous combined hormone therapy, even for long periods of time, is not associated with an increased risk of endometrial cancer.
2007 - Elsevier BV | American Journal of Obstetrics and Gynecology