Artyom Kachanov, Anastasiya Kostyusheva, Sergey Brezgin, Ivan Karandashov, N. I. Ponomareva, Andrey Tikhonov, Alexander N. Lukashev, Vadim S. Pokrovsky, Andrey A. Zamyatnin, Alessandro Parodi, Vladimir Chulanov, Dmitry Kostyushev,
Abstract Over the past decade, in vivo gene replacement therapy has significantly advanced, resulting in market approval of numerous therapeutics predominantly relying on adeno‐associated viral vectors (AAV). While viral vectors have undeniably addressed several critical healthcare challenges, their clinical application has unveiled a range of limitations and safety concerns. This review highlights the emerging challenges in the field of gene therapy. At first, we discuss both the role of biological ...
Tópico(s): CRISPR and Genetic Engineering
2024 - Wiley | Medicinal Research Reviews
Igor Yu. Kaplin, Е. С. Локтева, К. И. Маслаков, Artem V. Tikhonov, Andrey N. Kharlanov, A. V. Fionov, Alexey O. Kamaev, O. Ya. Isaikina, Sergey V. Maksimov, Е. В. Голубина,
In this work mesoporous CeO2-SiO2 catalysts with the Ce:Si molar ratios of 1:1 and 4:1 were synthesized by template method and modified with copper to elucidate the role of copper-ceria and ceria-silica interactions in the catalyst efficiency in CO-PROX. The catalysts were characterized by SEM-EDS, AAS, XRD, TPR-H2, TEM, and spectroscopic methods (Raman, EPR and in situ DRIFT of adsorbed CO). The binary catalyst with the equimolar Ce:Si ratio demonstrated better catalytic performance in CO-PROX due ...
Tópico(s): Mesoporous Materials and Catalysis
2022 - Elsevier BV | Applied Surface Science
Annamaria Cucina, Antonella Di Francesco, Rosaria Saletti, Maria Gaetana Giovanna Pittalà, Gleb Zilberstein, Svetlana Zilberstein, Alexei Tikhonov, Andrey G. Bublichenko, Pier Giorgio Righetti, Salvatore Foti, Vincenzo Cunsolo,
The recent paleoproteomic studies, including paleo-metaproteomic analyses, improved our understanding of the dietary of ancient populations, the characterization of past human diseases, the reconstruction of the habitat of ancient species, but also provided new insights into the phylogenetic relationships between extant and extinct species. In this respect, the present work reports the results of the metaproteomic analysis performed on the middle part of a trunk, and on the portion of a trunk tip ...
Tópico(s): Forensic and Genetic Research
2022 - Springer Science+Business Media | Amino Acids
Alexey Efimov, Pavel V. Arsenov, В И Борисов, Arseny I. Buchnev, Anna A. Lizunova, Denis Kornyushin, Sergey Tikhonov, Andrey Musaev, Maxim Urazov, М. А. Щербаков, Д. Е. Спирин, Victor Ivanov,
A cost-effective, scalable and versatile method of preparing nano-ink without hazardous chemical precursors is a prerequisite for widespread adoption of printed electronics. Precursor-free synthesis by spark discharge is promising for this purpose. The synthesis of platinum nanoparticles (PtNPs) using a spark discharge under Ar, N2, and air has been investigated to prepare highly conductive nano-ink. The size, chemical composition, and mass production rate of PtNPs significantly depended on the carrier ...
Tópico(s): Gold and Silver Nanoparticles Synthesis and Applications
2021 - Multidisciplinary Digital Publishing Institute | Nanomaterials
Annamaria Cucina, Vincenzo Cunsolo, Antonella Di Francesco, Rosaria Saletti, Gleb Zilberstein, Svetlana Zilberstein, Alexei Tikhonov, Andrey G. Bublichenko, Pier Giorgio Righetti, Salvatore Foti,
During the last decade, paleoproteomics allowed us to open a direct window into the biological past, improving our understanding of the phylogenetic relationships of extant and extinct species, past human diseases, and reconstruction of the human diet. In particular, meta-proteomic studies, mainly carried out on ancient human dental calculus, provided insights into past oral microbial communities and ancient diets. On the contrary, very few investigations regard the analysis of ancient gut microbiota, ...
Tópico(s): Nutritional Studies and Diet
2021 - Springer Science+Business Media | Amino Acids
Andrey Tikhonov, Artyom Kachanov, Alexandra Yudaeva, Oleg Danilik, N. I. Ponomareva, Ivan Karandashov, Anastasiya Kostyusheva, Andrey A. Zamyatnin, Alessandro Parodi, Vladimir Chulanov, Sergey Brezgin, Dmitry Kostyushev,
Biomimetic nanoparticles (BMNPs) are innovative nanovehicles that replicate the properties of naturally occurring extracellular vesicles, facilitating highly efficient drug delivery across biological barriers to target organs and tissues while ensuring maximal biocompatibility and minimal-to-no toxicity. BMNPs can be utilized for the delivery of therapeutic payloads and for imparting novel properties to other nanotechnologies based on organic and inorganic materials. The application of specifically ...
Tópico(s): Extracellular vesicles in disease
2024 - Multidisciplinary Digital Publishing Institute | Pharmaceutics
Tom Westerhout, Nikita Astrakhantsev, K. S. Tikhonov, M. I. Katsnelson, Andrey A. Bagrov,
Abstract Neural quantum states (NQS) attract a lot of attention due to their potential to serve as a very expressive variational ansatz for quantum many-body systems. Here we study the main factors governing the applicability of NQS to frustrated magnets by training neural networks to approximate ground states of several moderately-sized Hamiltonians using the corresponding wave function structure on a small subset of the Hilbert space basis as training dataset. We notice that generalization quality, ...
Tópico(s): Machine Learning in Materials Science
2020 - Nature Portfolio | Nature Communications
Sergey A. Tikhonov, Andrey E. Sidorin, Ilya S. Samoilov, Aleksandr V. Borisenko, V. I. Vovna,
Tópico(s): Photochemistry and Electron Transfer Studies
2020 - Elsevier BV | Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy
Misha Kapushesky, Tomasz Adamusiak, Tony Burdett, Aedín C. Culhane, Alessandro Farné, А. В. Филиппов, Ele Holloway, А. А. Клебанов, Nataliya Kryvych, Natalja Kurbatova, Pavel Kurnosov, James Malone, Olga Melnichuk, Robert Petryszak, N. Pultsin, Gabriella Rustici, A. Tikhonov, Ravensara S. Travillian, Eleanor Williams, Andrey Zorin, Helen Parkinson, Alvis Brāzma,
Gene Expression Atlas (http://www.ebi.ac.uk/gxa) is an added-value database providing information about gene expression in different cell types, organism parts, developmental stages, disease states, sample treatments and other biological/experimental conditions. The content of this database derives from curation, re-annotation and statistical analysis of selected data from the ArrayExpress Archive and the European Nucleotide Archive. A simple interface allows the user to query for differential gene ...
Tópico(s): Biomedical Text Mining and Ontologies
2011 - Oxford University Press | Nucleic Acids Research
Sergey A. Milenin, Elizaveta V. Selezneva, P. A. Tikhonov, В. Г. Васильев, A. I. Buzin, Н. К. Балабаев, Andrey O. Kurbatov, Maxim V. Petoukhov, Eleonora V. Shtykova, L. A. Feĭgin, Е. А. Татаринова, Elena Yu. Kramarenko, С. Н. Чвалун, Aziz M. Muzafarov,
A series of carbosilane dendrimers of the 4th, 6th, and 7th generations with a terminal trimethylsilylsiloxane layer was synthesized. Theoretical models of these dendrimers were developed, and equilibrium dendrimer conformations obtained via molecular dynamics simulations were in a good agreement with experimental small-angle X-ray scattering (SAXS) data demonstrating molecule monodispersity and an almost spherical shape. It was confirmed that the glass transition temperature is independent of the ...
Tópico(s): Polymer crystallization and properties
2021 - Multidisciplinary Digital Publishing Institute | Polymers
Anastasiya Kostyusheva, Sergey Brezgin, Н.В. Пономарева, Anastasiia Frolova, Aleksandr S. Lunin, Ekaterina Bayurova, Andrey Tikhonov, O. V. Slatinskaya, Polina A. Demina, Artyom Kachanov, Gulalek Babayeva, Irina I. Khan, Д. А. Хоченков, Yulia Khochenkova, Darina V. Sokolova, Д. Н. Силачев, Г. В. Максимов, E. V. Khaydukov, Vadim S. Pokrovsky, Andrey A. Zamyatnin, Alessandro Parodi, Ilya Gordeychuk, Vladimir Chulanov, Dmitry Kostyushev,
Tópico(s): CRISPR and Genetic Engineering
2024 - Elsevier BV | Molecular Therapy
Tatiana Kuznetsova, Alexey Schulga, A. N. Wulfson, Jan S. Keruchenko, Yaroslav S. Ermolyuk, Irina D. Keruchenko, Roman V. Tikhonov, K. V. Lisitskaya, Andrey A. Makarov, Katya Chobotova, Vasily G. Khomenkov, Vyacheslav P. Khotchenkov, Vladimir O. Popov, М. П. Кирпичников, А. Б. Шевелев,
MGF is a product of a unique muscle-specific splice variant of IGF1 gene (insulin-like growth factor). Its peculiar feature is a specific E-peptide, a 16 a.a. strand at the C-terminus. MGF increases cellular proliferation and inhibits terminal differentiation of myoblasts necessary for the secondary myotube formation. Previous analysis of physiological effects of MGF was performed using indirect methods such as RT-PCR based examination of the transcript contents in normal tissues, adenovirus-mediated ...
Tópico(s): Genetic Neurodegenerative Diseases
2007 - Elsevier BV | Protein Expression and Purification
Sergey A. Tikhonov, Andrey E. Sidorin, Alexander A. Ksenofontov, D.Yu. Kosyanov, Ilya S. Samoilov, A. D. Skitnevskaya, А. Б. Трофимов, Е. В. Антина, М. Б. Березин, V. I. Vovna,
The results of a X-ray photoelectron spectroscopy (XPS) and steady-state absorption spectroscopy study of the electronic structure, and cationic and excited states of a series of 1,3,5,7-tetramethyl-substituted BODIPYs (4Me,2R-BODIPYs) are presented. The experimental data were interpreted using high-level ab initio quantum chemical computations, including the algebraic diagrammatic construction method for the polarization propagator of the second order (ADC(2)), the outer-valence Green's function (OVGF) ...
Tópico(s): Porphyrin and Phthalocyanine Chemistry
2023 - Royal Society of Chemistry | Physical Chemistry Chemical Physics
Olga G. Chiryaeva, Anna Andreevna Pendina, Andrey V. Tikhonov, Olga A. Efimova, L Petrova, Vera S. Dudkina, Natalia Aleksandrovna Sadik, Tatyana V. Kuznetzova, Владислав Сергеевич Баранов, Ilona A. Galembo,
The present paper summarizes results of cytogenetic study of 679 chorionic samples from miscarriages following natural conception and assisted reproductive technologies. Frequency and spectrum of karyotype pathology and its correlation with maternal age and term of gestation are analyzed. The results are compared and discussed with relevant other studies
Tópico(s): Assisted Reproductive Technology and Twin Pregnancy
2012 - ECO-vector | Journal of obstetrics and women s diseases
Vadim S. Tikhonov, V.I. Lebedev, Andrey V. Kotov,
A mathematical model is proposed for longitudinal oscillations of a tackle used for salvaging sunken vessels to the sea surface. The numerical approximation of the obtained model along the tackle rope length is accomplished by means of the finite-difference method. To implement this method, the transformation of independent coordinates is introduced into the constant step mesh. The integration with respect to time is carried out by the Φ-Wilson method. To solve the nonlinear systems of algebraic ...
Tópico(s): Stonefly species taxonomy and ecology
1997 - Elsevier BV | Ocean Engineering
Sergey Brezgin, Anastasia S. Frolova, Ekaterina Bayurova, O. V. Slatinskaya, Н.В. Пономарева, E. Yu. Parshina, Zhanna V. Bochkova, Artyom Kachanov, Andrey Tikhonov, Anastasiya Kostyusheva, Ivan Karandashov, Polina A. Demina, Oleg Latyshev, Olesja Eliseeva, Marina Belikova, Vadim S. Pokrovsky, V. I. Gegechkory, E. V. Khaydukov, Д. Н. Силачев, Andrey A. Zamyatnin, Г. В. Максимов, Alexander N. Lukashev, Ilya Gordeychuk, Vladimir Chulanov, Dmitry Kostyushev,
Extracellular vesicles (EVs), biomimetics, and other biological nanoparticles (BNs) produced from human cells are gaining increasing attention in the fields of molecular diagnostics and nanomedicine for the delivery of therapeutic cargo. In particular, BNs are considered prospective delivery vehicles for different biologics, including protein and RNA therapeutics. Moreover, EVs are widely used in molecular diagnostics for early detection of disease-associated proteins and RNA. Technical approaches ...
Tópico(s): Galectins and Cancer Biology
2024 - American Chemical Society | Analytical Chemistry
Olga V. Orlova, Д. В. Глазкова, Г. М. Цыганова, Irina V. Antoshkina, R. R. Mintaev, Andrey Tikhonov, E. V. Bogoslovskaya, German A. Shipulin,
Obtaining a pure recombinant Modified Vaccinia Ankara (MVA) virus is a multistage, time-consuming procedure. We describe a novel single-tube real-time PCR which enables determination of the amount of wild type and recombinant viruses and their ratio in plaques. Use of the real-time PCR significantly reduce the time and efforts needed to obtain purified recombinant MVA. The new approach has been applied to generate recombinant MVAs encoding different SARS-COV-2 antigens.
Tópico(s): Virus-based gene therapy research
2020 - Elsevier BV | Journal of Virological Methods
Andrey D. Grigoriev, R. V. Salimov, R. I. Tikhonov,
Tópico(s): Electromagnetic Scattering and Analysis
2012 - Pleiades Publishing | Journal of Communications Technology and Electronics
Saeed Rahmanian Koshkaki, Zahed Allahyari, Artem R. Oganov, Vladimir L. Solozhenko, Ilya B. Polovov, А. С. Белозеров, A. A. Katanin, В. И. Анисимов, Evgenii Tikhonov, Guang‐Rui Qian, Konstantin V. Maksimtsev, Andrey S. Mukhamadeev, А. В. Чукин, А. В. Королев, Н.В. Мушников, Hao Li,
The discovery of new magnetic materials is a big challenge in the field of modern materials science. We report the development of a new extension of the evolutionary algorithm USPEX, enabling the search for half-metals (materials that are metallic only in one spin channel) and hard magnetic materials. First, we enabled the simultaneous optimization of stoichiometries, crystal structures, and magnetic structures of stable phases. Second, we developed a new fitness function for half-metallic materials ...
Tópico(s): Advancements in Battery Materials
2022 - American Institute of Physics | The Journal of Chemical Physics
Kirill Khabarov, Emiliia Filalova, Messan Nouraldeen, Ekaterina Kameneva, Andrey Musaev, Sergei Tikhonov, Victor Ivanov,
An analyzed substance's signal intensity and detection sensitivity in surface-enhanced Raman spectroscopy (SERS) significantly depend on the size and agglomeration degree of nanoparticles (NPs) forming the enhancing structure. Structures were manufactured by aerosol dry printing (ADP), where NPs' agglomeration depends on printing conditions and additional particle modification techniques. The influence of agglomeration degree on SERS signal enhancement was studied in three types of printed structures ...
Tópico(s): Spectroscopy Techniques in Biomedical and Chemical Research
2023 - Multidisciplinary Digital Publishing Institute | Nanomaterials
Daniiar Dyikanov, A. V. Zaitsev, Т.В. Васильева, Iris Wang, Arseniy A. Sokolov, Evgenii S Bolshakov, Alena Frank, Polina Turova, Olga Golubeva, Anna Gantseva, Anna Kamysheva, Polina Shpudeiko, Ilya E. Krauz, Mary Abdou, Madison Chasse, Tori Conroy, Nicholas R. Merriam, Julia E. Alesse, Noel English, Boris Shpak, Anna Shchetsova, Evgenii Tikhonov, I. V. Filatov, Anastasia Radko, Anastasiia A. Bolshakova, Anastasia I. Kachalova, Nika Lugovykh, Andrey Bulahov, Anastasiia Kilina, Syimyk Asanbekov, Irina Zheleznyak, Pavel Skoptsov, E. A. Alekseeva, Jennifer M. Johnson, Joseph Curry, Alban J. Linnenbach, Andrew P. South, EnJun Yang, Kirill Morozov, Anastasiya Terenteva, Lira Nigmatullina, Dmitry Fastovetz, Anatoly Bobe, Linda Balabanian, Krystle Nomie, Sheila T. Yong, Christopher J.H. Davitt, Alexander A. Ryabykh, Olga Kudryashova, Cagdas Tazearslan, Alexander Bagaev, Nathan Fowler, Adam Luginbuhl, Ravshan Ataullakhanov, Michael Goldberg,
The lack of comprehensive diagnostics and consensus analytical models for evaluating the status of a patient's immune system has hindered a wider adoption of immunoprofiling for treatment monitoring and response prediction in cancer patients. To address this unmet need, we developed an immunoprofiling platform that uses multiparameter flow cytometry to characterize immune cell heterogeneity in the peripheral blood of healthy donors and patients with advanced cancers. Using unsupervised clustering, ...
Tópico(s): Cancer Immunotherapy and Biomarkers
2024 - Cell Press | Cancer Cell
I. A. Yamskov, Andrey N. Kuskov, Kirill K. Babievsky, Boris B. Berezin, M. A. Krayukhina, Natalia A. Samoylova, Vladimir E. Tikhonov, М.И. Штильман,
Tópico(s): Advanced Drug Delivery Systems
2008 - Pleiades Publishing | Applied Biochemistry and Microbiology
Р. В. Михайлова, V. G. Semenov, A. S. Tikhonov, Andrey E. Makushev, K. V. Fadeeva, O. N. Fedorova, Михаил Анатольевич Пронин, Marina I. Ivleva, A. S. Kornilova,
Relevance. Agrotourism is an actively developing sector in the system of modern tourism. Its development requires an in-depth study of the problems associated with it. Agrotourism as a form of man's relationship to nature in the cultural aspect is focused on the development of man as a person (as an object and subject). In the civilized aspect, it contributes to the implementation of a responsible attitude of man to nature based on the principle of utility. Agrotourism contains the potential for ...
Tópico(s): Regional Economic Development and Innovation
2023 - | Agrarian science
N. V. Kudryashov, А. А. Горбунов, Н.Б. Свиридкина, Sergey E. Mironov, D. A. Tikhonov, Andrey Nedorubov, Владимир Петрович Фисенко,
Introduction: Dimethyl sulfoxide (DMSO) is a commonly used solvent that can be applied in experimental studies for preparation of hydrophobic solutions as well as in capacity of a cryopreservative in transplantology. According to modern data acquired from in vitro experiments, DMSO is able to change the structure of myelin by decreasing synthesis of its main components and inhibiting oligodendrocyte genesis. Aim of the study: We studied influence of DMSO on anxiety and compulsive-like behavior, pain ...
Tópico(s): Neurogenesis and neuroplasticity mechanisms
2023 - Pensoft Publishers | Research Results in Pharmacology
А.С. Алексеев, S. V. Tikhonov, Andrey Shcherbakov,
Tópico(s): Forest Ecology and Biodiversity Studies
1992 - Nauka | БОТАНИЧЕСКИЙ ЖУРНАЛ
A. I. Tikhonov, Andrey Karzhevin, A. Golubev, А.V. Stulov,
Tópico(s): Magnetic Field Sensors Techniques
2023 - Pleiades Publishing | Russian Electrical Engineering
Mikhail I. Krapivin, Yanina M. Sagurova, Olga A. Efimova, Andrey V. Tikhonov, Anna A. Pendina,
In this review, we summarize data on the structural and functional characteristics of human telomeres and analyze how endo- and exogenous factors influence telomere length. We elucidate the history of telomere investigation, describe their structure and functions, methods of their study. We also characterize the mechanisms of telomere lengthening and shortening. We discuss in detail endo- and exogenous factors affecting telomere length during gametogenesis, embryogenesis and in the postnatal period ...
Tópico(s): Telomeres, Telomerase, and Senescence
2022 - | Ecological genetics
María del Mar Delgado, Gleb Tikhonov, Evgeniy Meyke, Miroslav Babushkin, Tatjana Bespalova, Svetlana Bondarchuk, Alexandra Yu. Esengeldenova, Irina Fedchenko, Yury Kalinkin, Anastasia Knorre, Gennadiy Kosenkov, Vladimir Kozsheechkin, A. V. Kuznetsov, Evgeniy Larin, Danis Mirsaitov, Irina Prokosheva, Yuri Rozhkov, Andrey Rykov, Ivan V. Seryodkin, Sergej Shubin, Rustam Sibgatullin, Natalia Sikkilä, Elena Sitnikova, Lilija Sultangareeva, Aleksander Vasin, L. Yarushina, Juri Kurhinen, Vincenzo Penteriani,
For brown bears (Ursus arctos), hibernation is a critical part of the annual life cycle because energy savings during hibernation can be crucial for overwintering, and females give birth to cubs at that time. For hibernation to be a useful strategy, timing is critical. However, environmental conditions vary greatly, which might have a negative effect on the functionality of the evolved biological time-keeping. Here, we used a long-term dataset (69 years) on brown bear denning phenology recorded in ...
Tópico(s): Effects of Environmental Stressors on Livestock
2018 - BioMed Central | Frontiers in Zoology
Andrey V. Mardanov, Е. С. Булыгина, Artem Nedoluzhko, Vitaly V. Kadnikov, А. В. Белецкий, Svetlana Tsygankova, Alexei Tikhonov, Nikolai V. Ravin, Е. Б. Прохорчук, K. G. Skryabin,
Tópico(s): Agriculture Sustainability and Environmental Impact
2012 - Pleiades Publishing | Doklady Biochemistry and Biophysics
Evgeniya A. Bezrodnykh, Boris B. Berezin, Yury A. Antonov, I. L. Zhuravleva, Anastasiia Atamas, Aleksei A. Tsarenko, Andrey Rogachev, Vladimir E. Tikhonov,
Interaction of binary chitosan/nonionic surfactant (NIS) system with sodium dodecyl sulfate (SDS) in aqueous solution is described using turbodimetry, light scattering, electophoretic mobility and cryogenic electron microscopy. The formation of insoluble CHI/SDS complexes is weakened with a decrease in molecular weight of chitosan and critical micelle concentration of NIS as well as with an increase in NIS concentration. Soluble chitosan/NIS complexes absorb SDS molecules until the charge of mixed ...
Tópico(s): Advanced Drug Delivery Systems
2022 - Elsevier BV | Carbohydrate Polymers