Produção Nacional
Revisado por pares
Gabriel Antunes de Araújo, Ana Lívia Agostinho, Alfredo Christofoletti Silveira, Shirley Freitas, Manuele Bandeira,
O isolamento da ilha de Ano Bom, na Guiné Equatorial, e as condições sócio-históricas de seu povoamento permitiram o surgimento de duas línguas: o fa d’ambô ... de base lexical portuguesa falado na Ilha de Ano Bom, na Guiné Equatorial. Em seguida, mostramos que o ... a única herança linguística portuguesa na Ilha de Ano Bom, uma vez que, até os dias atuais, os ... religiosas. Embora a colonização portuguesa na Ilha de Ano Bom tenha sido irregular, os fatos linguísticos e culturais, ... características únicas da civilização portuguesa na Ilha de Ano Bom. Mostraremos que o estudo das línguas da Ilha ...
Tópico(s): African history and culture studies
2013 - UNIVERSIDADE ESTADUAL DE CAMPINAS | Cadernos de Estudos Lingüísticos
Produção Nacional
Revisado por pares
Ana Paula Dinis Ano Bom, Mônica S. Freitas, Flavia S. Moreira, Danielly Ferraz, Daniel Sanches, André M. O. Gomes, Ana Paula Valente, Yraima Cordeiro, Jerson L. Silva,
p53 is a transcription factor that maintains genome integrity, and its function is lost in 50% of human cancers. The majority of p53 mutations are clustered within the core domain. Here, we investigate the effects of low pH on the structure of the wild-type (wt) p53 core domain (p53C) and the R248Q mutant. At low pH, the tryptophan residue is partially exposed to the solvent, suggesting a fluctuating tertiary structure. On the other hand, the secondary structure increases, as determined by circular ...
Tópico(s): Epigenetics and DNA Methylation
2009 - Elsevier BV | Journal of Biological Chemistry
Produção Nacional
Revisado por pares
Daniella Ishimaru, Ana Paula Dinis Ano Bom, Luís Maurício T. R. Lima, Pablo A. Quesado, Marcos F. C. Oyama, Claúdia Vitória de Moura Gallo, Yraima Cordeiro, Jerson L. Silva,
The tumor suppressor protein p53 is a nuclear protein that serves as an important transcription factor. The region responsible for sequence-specific DNA interaction is located in its core domain (p53C). Although full-length p53 binds to DNA as a tetramer, p53C binds as a monomer since it lacks the oligomerization domain. It has been previously demonstrated that two core domains have a dimerization interface and undergo conformational change when bound to DNA. Here we demonstrate that the interaction ...
Tópico(s): DNA Repair Mechanisms
2009 - American Chemical Society | Biochemistry
Produção Nacional
Revisado por pares
Jerson L. Silva, Tuane C. R. G. Vieira, Mariana P. B. Gomes, Ana Paula Ano Bom, Luís Maurício T. R. Lima, Mônica S. Freitas, Daniella Ishimaru, Yraima Cordeiro, Débora Foguel,
Protein misfolding has been implicated in a large number of diseases termed protein- folding disorders (PFDs), which include Alzheimer's disease, Parkinson's disease, transmissible spongiform encephalopathies, familial amyloid polyneuropathy, Huntington's disease, and type II diabetes. In these diseases, large quantities of incorrectly folded proteins undergo aggregation, destroying brain cells and other tissues. The interplay between ligand binding and hydration is an important component of the ...
Tópico(s): Trace Elements in Health
2009 - American Chemical Society | Accounts of Chemical Research
Produção Nacional
Revisado por pares
Luciane P. Gaspar, Ana C. B. Silva, André M. O. Gomes, Mônica S. Freitas, Ana Paula Dinis Ano Bom, Waleska Dias Schwarcz, Jiří Městecký, Miroslav Novák, Débora Foguel, Jerson L. Silva,
Enveloped animal viruses must undergo membrane fusion to deliver their genome into the host cell. We demonstrate that high pressure inactivates two membrane-enveloped viruses, influenza and Sindbis, by trapping the particles in a fusion-intermediate state. The pressure-induced conformational changes in Sindbis and influenza viruses were followed using intrinsic and extrinsic fluorescence spectroscopy, circular dichroism, and fusion, plaque, and hemagglutination assays. Influenza virus subjected ...
Tópico(s): Protein Structure and Dynamics
2002 - Elsevier BV | Journal of Biological Chemistry
Produção Nacional
Revisado por pares
Claudia B. Levy, Ana Carolina Stumbo, Ana Paula Dinis Ano Bom, Elisabeth A. Portari, Yraima Carneiro, Jerson L. Silva, Claudia Vitória de Moura-Gallo,
P53 is one of the most important tumor suppressor proteins in human cancers. Mutations in the TP53 gene are common features of malignant tumors and normally correlate to a more aggressive disease. In breast cancer, these gene alterations are present in approximately 20% of cases and are characteristically of missense type. In the present work we describe TP53 mutations in breast cancer biopsies and investigate whether wild and mutant p53 participate in protein aggregates formation in these breast ...
Tópico(s): Microtubule and mitosis dynamics
2010 - Elsevier BV | The International Journal of Biochemistry & Cell Biology
Produção Nacional
Revisado por pares
Lara Barros Martins, Nina Rosa do Amaral Costa, Maria Clotilde T. Rossetti-Ferreira,
... 84,1%); faixa etária entre 30 e 49 anos; bom nível de escolarização; profissões autônomas; rendas diversificadas e ...
Tópico(s): Family and Disability Support Research
2010 - UNIVERSIDADE DE SÃO PAULO | Paidéia (Ribeirão Preto)
Produção Nacional
Revisado por pares
Carina Margonari, E. D. Silva, Ana Paula Dinis Ano Bom, Renata Bastos, Hilton Nascimento, J. G. Da SILVA,
The present work describes the isolation and purification of two Leishmania chagasi (= syn. Leishmania infantum) recombinant proteins, rLci2B and rLci1A, and their use in the development of an immunoassay for the diagnostic of canine leishmaniasis. After protein expression and cell disruption, rLci2B was purified by immobilized metal affinity chromatography followed by size exclusion chromatography, whereas rLci1A, expressed as an inclusion body, was treated with urea and purified by anion-exchange ...
Tópico(s): Vector-borne infectious diseases
2011 - Wiley | Parasite Immunology
Produção Nacional
Revisado por pares
Ana Paula Dinis Ano Bom, Luciana P. Rangel, Danielly C. Ferraz da Costa, Guilherme A. P. de Oliveira, Daniel Sanches, Carolina Braga, Lisandra M. Gava, Carlos H.I. Ramos, Ana O.T. Cepeda, Ana Carolina Stumbo, Claúdia Vitória de Moura Gallo, Yraima Cordeiro, Jerson L. Silva,
Over 50% of all human cancers lose p53 function. To evaluate the role of aggregation in cancer, we asked whether wild-type (WT) p53 and the hot-spot mutant R248Q could aggregate as amyloids under physiological conditions and whether the mutant could seed aggregation of the wild-type form. The central domains (p53C) of both constructs aggregated into a mixture of oligomers and fibrils. R248Q had a greater tendency to aggregate than WT p53. Full-length p53 aggregated into amyloid-like species that bound ...
Tópico(s): Prion Diseases and Protein Misfolding
2012 - Elsevier BV | Journal of Biological Chemistry
Produção Nacional
Revisado por pares
Cibele Bragagnolo, Ricardo Pinto‐da‐Rocha,
... are described: P. ale sp. n. (type locality: Ano Bom, Santa Catarina); P. trapista sp. n. (typelocality: Reserva ...
Tópico(s): Ichthyology and Marine Biology
2012 - Q15088586 | Zootaxa
Produção Nacional
Revisado por pares
Daniella Ishimaru, Luís Maurício T. R. Lima, Lenize F. Maia, Priscila M. Lopez, Ana Paula Ano Bom, Ana Paula Valente, Jerson L. Silva,
Tópico(s): Bacteriophages and microbial interactions
2004 - Elsevier BV | Biophysical Journal
Produção Nacional
Revisado por pares
Luciana Facchinetti de Castro Girão, Surza Lucia Gonçalves da Rocha, Ricardo Sposina Sobral, Ana Paula Dinis Ano Bom, André Luiz Franco Sampaio, José Godinho da Silva, Maria Antonieta Ferrara, Elba P. S. Bon, Jonás Perales,
Asparaginase obtained from Escherichia coli and Erwinia chrysanthemi are used to treat acute lymphocytic leukaemia and non-Hodgkin's lymphoma. However, these agents cause severe adverse effects. Saccharomyces cerevisiae asparaginase II, encoded by the ASP3 gene, could be a potential candidate for the formulation of new drugs. This work aimed to purify and characterize the periplasmic asparaginase produced by a recombinant Pichia pastoris strain harbouring the ASP3 gene. The enzyme was purified to ...
Tópico(s): DNA Repair Mechanisms
2015 - Elsevier BV | Protein Expression and Purification
Produção Nacional
Revisado por pares
Ana Paula Dinis Ano Bom, Patrícia Neves, Carlos Eduardo Bonacossa de Almeida, Dı́lson Silva, Sotiris Missailidis,
Both aptamers and siRNA technologies have now reached maturity, and both have been validated with a product in the market. However, although pegaptanib reached the market some time ago, there has been a slow process for new aptamers to follow. Today, some 40 aptamers are in the market, but many in combination with siRNAs, in the form of specific delivery agents. This combination offers the potential to explore the high affinity and specificity of aptamers, the silencing power of siRNA, and, at times, ...
Tópico(s): MicroRNA in disease regulation
2019 - Multidisciplinary Digital Publishing Institute | Pharmaceutics
Produção Nacional
Revisado por pares
Filipe Vieira Santos de Abreu, Anielly Ferreira-de-Brito, Adriana de Souza Azevedo, José Linhares, Vanessa de Oliveira Santos, Emily Hime Miranda, Maycon Sebastião Alberto Santos Neves, Léna Yousfi, Ieda Pereira Ribeiro, Alexandre Araújo Cunha dos Santos, Edmilson dos Santos, Taissa Pereira dos Santos, Danilo Simonini Teixeira, Marcelo Quintela Gomes, Camilla Bayma Fernandes, Andréa Marques Vieira da Silva, Monique da Rocha Queiroz Lima, Christophe Paupy, Alessandro Romano, Ana Paula Dinis Ano Bom, Luzia Maria de Oliveira Pinto, Sara Moutailler, Monique de Albuquerque Motta, María G. Castro, Myrna C. Bonaldo, Sheila Maria Barbosa de Lima, Ricardo Lourenço‐de‐Oliveira,
In the last decade, Flaviviruses such as yellow fever (YFV) and Zika (ZIKV) have expanded their transmission areas. These viruses originated in Africa, where they exhibit both sylvatic and interhuman transmission cycles. In Brazil, the risk of YFV urbanization has grown, with the sylvatic transmission approaching the most densely populated metropolis, while concern about ZIKV spillback to a sylvatic cycle has risen. To investigate these health threats, we carried out extensive collections and arbovirus ...
Tópico(s): COVID-19 epidemiological studies
2020 - Multidisciplinary Digital Publishing Institute | Viruses
Produção Nacional
Revisado por pares
Juliana Gil Melgaço, Tamiris Azamor, Ana Paula Dinis Ano Bom,
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces a severe acute respiratory syndrome that is called COVID-19. Clinical manifestations of COVID-19 include diarrhea, pneumonia, lymphopenia, exhausted lymphocytes, and pro-inflammatory cytokine production. Immunology is part of the process of clinical evolution, but there are some questions around immunity-based protection: (1) why some infected people have only mild symptoms of the disease or are asymptomatic; (2) why delayed and weak ...
Tópico(s): Long-Term Effects of COVID-19
2020 - Elsevier BV | Cellular Immunology
Produção Nacional
Revisado por pares
Izabela Maurício de Rezende, Leonardo Soares Pereira, Jordana Rodrigues Barbosa Fradico, Marcelo Antônio Pascoal-Xavier, Pedro Augusto Alves, Ana Carolina Campi‐Azevedo, Elaine Speziali, Lívia Zignago Moreira dos Santos, Natália Soares Albuquerque, Indiara Penido, Tayrine Araújo Santos, Ana Paula Dinis Ano Bom, Andréa Marques Vieira da Silva, Camilla Bayma Fernandes, Carlos Eduardo Calzavara-Silva, Erna Geessien Kroon, Olindo Assis Martins‐Filho, Andréa Teixeira−Carvalho, Betânia Paiva Drumond,
One patient presented hyporexia, asthenia, adynamia, and jaundice two months after acute yellow fever (YF) onset; plus laboratory tests indicating hepatic cytolysis and a rebound of alanine and aspartate transaminases, and total and direct bilirubin levels. Laboratory tests discarded autoimmune hepatitis, inflammatory or metabolic liver disease, and new infections caused by hepatotropic agents. Anti-YFV IgM, IgG and neutralizing antibodies were detected in different times, but no viremia. A liver ...
Tópico(s): Viral Infections and Vectors
2020 - Multidisciplinary Digital Publishing Institute | Viruses
Produção Nacional
Revisado por pares
Tamiris Azamor, Daniela P. Cunha, Andréa Marques Vieira da Silva, Ohanna Cavalcanti de Lima Bezerra, Marcelo Ribeiro-Alves, Thyago Leal-Calvo, Fernanda S. G. Kehdy, Fernanda Saloum de Neves Manta, Thiago Gomes de Toledo Pinto, Laís Pereira Ferreira, Elyzabeth Avvad Portari, Letícia da Cunha Guida, Leonardo Henrique Ferreira Gomes, Maria Elisabeth Lopes Moreira, Elizeu Fagundes de Carvalho, Cynthia Chester Cardoso, Marcelo Marques Lopes Müller, Ana Paula Dinis Ano Bom, Patrícia Neves, Zilton Vasconcelos, Milton Ozório Moraes,
Host factors that influence Congenital Zika Syndrome (CZS) outcome remain elusive. Interferons have been reported as the main antiviral factor in Zika and other flavivirus infections. Here, we accessed samples from 153 pregnant women (77 without and 76 with CZS) and 143 newborns (77 without and 66 with CZS) exposed to ZIKV conducted a case-control study to verify whether interferon alfa receptor 1 (IFNAR1) and interferon lambda 2 and 4 (IFNL2/4) single nucleotide polymorphisms (SNPs) contribute to CZS ...
Tópico(s): Parvovirus B19 Infection Studies
2021 - Frontiers Media | Frontiers in Immunology
Produção Nacional
Revisado por pares
Paul Bastard, Eleftherios Michailidis, Hans-Heinrich Hoffmann, Marwa Chbihi, Tom Le Voyer, Jérémie Rosain, Quentin Philippot, Yoann Seeleuthner, Adrian Gervais, Marie Materna, Patrícia Mouta Nunes de Oliveira, Maria de Lourdes S. Maia, Ana Paula Dinis Ano Bom, Tamiris Azamor, Deborah Araújo da Conceição, Ekaterini Goudouris, Akira Homma, Günther Slesak, Johannes Schäfer, Bali Pulendran, Joseph D. Miller, Ralph Huits, Rui Yang, Lindsey B. Rosen, Lucy Bizien, Lazaro Lorenzo, Maya Chrabieh, Lucía Victoria Erazo-Borrás, Flore Rozenberg, Mohamed Jeljeli, Vivien Béziat, Steven M. Holland, Aurélie Cobat, Luigi D. Notarangelo, Helen C. Su, Rafi Ahmed, Anne Puel, Shen‐Ying Zhang, Laurent Abel, Stephen Seligman, Qian Zhang, Margaret R. MacDonald, Emmanuelle Jouanguy, Charles M. Rice, Jean‐Laurent Casanova,
Yellow fever virus (YFV) live attenuated vaccine can, in rare cases, cause life-threatening disease, typically in patients with no previous history of severe viral illness. Autosomal recessive (AR) complete IFNAR1 deficiency was reported in one 12-yr-old patient. Here, we studied seven other previously healthy patients aged 13 to 80 yr with unexplained life-threatening YFV vaccine–associated disease. One 13-yr-old patient had AR complete IFNAR2 deficiency. Three other patients vaccinated at the ages ...
Tópico(s): Immune Cell Function and Interaction
2021 - Rockefeller University Press | The Journal of Experimental Medicine
Produção Nacional
Revisado por pares
André T. Fernandes, Sílvia Bahadian Moreira, Luciane P. Gaspar, Marisol Simões, Ana Carolina dos Reis Albuquerque Cajaraville, Renata Carvalho Pereira, Mariana P. B. Gomes, José Linhares, Vanessa de Oliveira Santos, Renata Tourinho Santos, Juliana Fernandes Amorim da Silva, Tamiris Azamor, Juliana Gil Melgaço, Andréa Marques Vieira da Silva, Camilla Bayma Fernandes, Luciana Tubarão, Jane da Silva, Elena Caride, Maria Beatriz Borges, Rosane Cuber Guimarães, Renato Sérgio Marchevsky, Sheila Maria Barbosa de Lima, Ana Paula Dinis Ano Bom, Patrícia Neves, Alcides Pissinatti, Marcos da Silva Freire,
Abstract Background Alouatta spp. are highly susceptible to yellow fever (YF) infection and develop an often fatal disease. The threat posed by an outbreak started in 2016 leads us to investigate vaccination as a potential tool in preventing YF in non‐human primates (NHP). Methods Susceptible howler monkeys were immunized with three different concentrations of the human Brazilian commercial YF17DD vaccine. Post‐vaccination viremia/RNAemia, immunogenicity, and safety were characterized. Results The ...
Tópico(s): Virology and Viral Diseases
2020 - Wiley | Journal of Medical Primatology
Produção Nacional
Revisado por pares
Tamiris Azamor, Andréa Marques Vieira da Silva, Juliana Gil Melgaço, Ana Paula Santos, Caroline Xavier-Carvalho, Lucia Elena Alvarado-Arnez, Leonardo Ribeiro Batista-Silva, Denise Matos, Camilla Bayma, Sotiris Missailidis, Ana Paula Dinis Ano Bom, Milton Ozório Moraes, Patrícia Neves,
The yellow fever vaccine (YF17DD) is highly effective with a single injection conferring protection for at least 10 years. The YF17DD induces polyvalent responses, with a TH1/TH2 CD4+ profile, robust T CD8+ responses, and synthesis of interferon-gamma (IFN-γ), culminating in high titers of neutralizing antibodies. Furthermore, C-type lectin domain containing 5A (CLEC5A) has been implicated in innate outcomes in other flaviviral infections. Here, we conducted a follow-up study in volunteers immunized ...
Tópico(s): Malaria Research and Control
2021 - Multidisciplinary Digital Publishing Institute | Viruses
Produção Nacional
Revisado por pares
Laise Rodrigues Reis, Ismael Artur da Costa-Rocha, Ana Carolina Campi‐Azevedo, Vanessa Peruhype-Magalhães, Jordana Grazziela Coelho-dos-Reis, Christiane Costa-Pereira, Dayane Andriotti Otta, Larissa Chaves Freire, Sheila Maria Barbosa de Lima, Adriana de Souza Azevedo, Waleska Dias Schwarcz, Ana Paula Dinis Ano Bom, Andréa Marques Vieira da Silva, Alessandro Fonseca de Souza, Thalita da Matta de Castro, Clara Lucy de Vasconcellos Ferroco, Ana María Bispo de Filippis, Fernanda de Bruycker Nogueira, Akira Homma, Carla Magda Allan Santos Domingues, Eduardo Sérgio Soares Sousa, Luiz Antônio Bastos Camacho, Maria de Lourdes Maia, Andréa Teixeira−Carvalho, Olindo Assis Martins‐Filho,
The present investigation comprised two independent observational arms to evaluate the influence of pre-existing flavivirus humoral immunity and the age-impact on 17DD-YF vaccination immunity. Flavivirus (YFV; DENV; ZIKV) serology and YF-specific cellular immunity was evaluated in 288 children/9Mths–4Yrs and 288 adults/18–49Yrs residents of areas without YFV circulation. Data demonstrated that flavivirus seropositivity at baseline was higher in Adults as compared to Children (26%;87%;67% vs 6%;13%; ...
Tópico(s): Vibrio bacteria research studies
2021 - Elsevier BV | Vaccine
Produção Nacional
Revisado por pares
Tamiris Azamor, Ingrid Horbach, Danielle Brito e Cunha, Juliana Gil Melgaço, Andréa Marques Vieira da Silva, Luciana Tubarão, Adriana de Souza Azevedo, Renata Tourinho Santos, Nathalia dos Santos Alves, Thiago Lazari Machado, Jane Silva, Alessandro Fonseca de Souza, Camilla Bayma, Vanessa Pimenta Rocha, Ana Frederico, Brenda de Moura Dias, Bruno Pimenta Setatino, Caio Bidueira Denani, Samir Pereira da Costa Campos, Waleska Dias Schwarcz, Michel Sucupira, Edinéa Pastro Mendes, Edimílson Domingos da Silva, Sheila Maria Barbosa de Lima, Ana Paula Dinis Ano Bom, Sotiris Missailidis,
Infections caused by SARS-CoV-2 induce a severe acute respiratory syndrome called COVID-19 and have led to more than six million deaths worldwide. Vaccination is the most effective preventative measure, and cellular and humoral immunity is crucial to developing individual protection. Here, we aim to investigate hybrid immunity against SARS-CoV-2 triggered by the ChAadOx1 nCoV-19 vaccine in a Brazilian cohort. We investigated the immune response from ChAadOx1 nCoV-19 vaccination in naïve (noCOVID- ...
Tópico(s): Vaccine Coverage and Hesitancy
2022 - Multidisciplinary Digital Publishing Institute | Viruses
Produção Nacional
Revisado por pares
Juliana Gil Melgaço, Danielle Brito e Cunha, Tamiris Azamor, Andréa Marques Vieira da Silva, Luciana Tubarão, Rafael Braga Gonçalves, Robson Q. Monteiro, Sotiris Missailidis, Patrícia Neves, Ana Paula Dinis Ano Bom,
The severe acute respiratory syndrome caused by the new coronavirus (SARS-CoV-2), termed COVID-19, has been highlighted as the most important infectious disease of our time, without a vaccine and treatment available until this moment, with a big impact on health systems worldwide, and with high mortality rates associated with respiratory viral disease. The medical and scientific communities have also been confronted by an urgent need to better understand the mechanism of host-virus interaction aimed ...
Tópico(s): Dermatological and COVID-19 studies
2020 - Hindawi Publishing Corporation | Journal of Immunology Research
Produção Nacional
Neusiene Medeiros da Silva, Anna Jéssica Pinto de Andrade, Cimone Rozendo,
... vida específico, reatualizando a esperança destes de um ano “bom de inverno”, mais do que organizando suas atividades ...
Tópico(s): Rural Development and Agriculture
2013 - UNIVERSIDADE FEDERAL DO PARANÁ | Desenvolvimento e Meio Ambiente
Produção Nacional
Revisado por pares
Cristiane Garboggini Melo de Pinheiro, Mayara de Oliveira Pedrosa, Naiara Carvalho Teixeira, Ana Paula Dinis Ano Bom, Monique M. van Oers, Geraldo Gileno de Sá Oliveira,
Interleukin-12 is an important cytokine in mediating cellular immune responses.Recombinant single-chain canine IL-12 was produced in a baculovirus-insect cell system with the aim of conducting further studies on modulation of immune responses in dogs. To optimize the production of recombinant canine IL-12, a classical baculovirus and a modified vector (chitinase A and v-cathepsin knockout) were used containing a native or an optimized insert of canine IL-12. The optimized IL-12 construct contained ...
Tópico(s): Transgenic Plants and Applications
2016 - BioMed Central | BMC Research Notes
Produção Nacional
Revisado por pares
Claudia B. Levy, Ana Carolina Stumbo, Ana Paula Dinis Ano Bom, Elisabeth A. Portari, Yraima Cordeiro, Jerson L. Silva, Claudia V. DeMoura-Gallo,
Tópico(s): 14-3-3 protein interactions
2011 - Elsevier BV | The International Journal of Biochemistry & Cell Biology
Produção Nacional
Revisado por pares
Mariana P. B. Gomes, José Linhares, Tiago Pereira Santos, Renata Carvalho Pereira, Renata Tourinho Santos, Stephanie Almeida da Silva, Marta Cristina Souza, Juliana Fernandes Amorim da Silva, Gisela Freitas Trindade, Viviane Silva Gomes, Débora Ferreira Barreto-Vieira, Milena Carvalho, Ana Paula Dinis Ano Bom, Noemi Rovaris Gardinali, Rodrigo Müller, Nathalia dos Santos Alves, Luma da Cruz Moura, Patrícia Neves, Gabriela S. Esteves, Waleska Dias Schwarcz, Sotiris Missailidis, Ygara S. Mendes, Sheila Maria Barbosa de Lima,
Successful SARS-CoV-2 inactivation allows its safe use in Biosafety Level 2 facilities, and the use of the whole viral particle helps in the development of analytical methods and a more reliable immune response, contributing to the development and improvement of in vitro and in vivo assays. In order to obtain a functional product, we evaluated several inactivation protocols and observed that 0.03% beta-propiolactone for 24 h was the best condition tested, as it promoted SARS-CoV-2 inactivation above ...
Tópico(s): Respiratory viral infections research
2023 - Multidisciplinary Digital Publishing Institute | Viruses
Ano Bom (nowadays known as Annobon, and part of Equatorial Guinea) is a small volcanic island with a ...
Tópico(s): Global Maritime and Colonial Histories
2009 - | Afro-Hispanic review
Produção Nacional
Revisado por pares
Nathalia dos Santos Alves, Adriana de Souza Azevedo, Brenda de Moura Dias, Ingrid Horbach, Bruno Pimenta Setatino, Caio Bidueira Denani, Waleska Dias Schwarcz, Sheila Maria Barbosa de Lima, Sotiris Missailidis, Ana Paula Dinis Ano Bom, Andréa Marques Vieira da Silva, Débora Ferreira Barreto-Vieira, Marcos Alexandre Nunes da Silva, Caroline A. Barros, Carlos Alberto Marques de Carvalho, Rafael Braga Gonçalves,
Despite the rapid mass vaccination against COVID-19, the emergence of new SARS-CoV-2 variants of concern, such as omicron, is still a great distress, and new therapeutic options are needed. Bovine lactoferrin (bLf), a multifunctional iron-binding glycoprotein available in unsaturated (apo-bLf) and saturated (holo-bLf) forms, has been shown to exert broad-spectrum antiviral activity against many viruses. In this study, we evaluated the efficacy of both forms of bLf at 1 mg/mL against infection of Vero ...
Tópico(s): COVID-19 Impact on Reproduction
2023 - Multidisciplinary Digital Publishing Institute | Pharmaceuticals
Produção Nacional
Revisado por pares
Juliana Gil Melgaço, Tamiris Azamor, Andréa Marques Vieira da Silva, José Linhares, Tiago Pereira Santos, Ygara S. Mendes, Sheila Maria Barbosa de Lima, Camilla Bayma Fernandes, Jane da Silva, Alessandro Fonseca de Souza, Luciana Tubarão, Danielle Brito e Cunha, Tamires Bomfim Santos Pereira, Catarina Eugênia Menezes, Milene Dias Miranda, Aline da Rocha Matos, Bráulia Costa Caetano, Jéssica Santa Cruz Carvalho Martins, Thyago Leal-Calvo, Natália Fintelman-Rodrigues, Carolina Q. Sacramento, Marilda Mendonça Siqueira, Milton Ozório Moraes, Sotiris Missailidis, Patrícia Neves, Ana Paula Dinis Ano Bom,
The cellular immune response plays an important role in COVID-19, caused by SARS-CoV-2. This feature makes use of in vitro models' useful tools to evaluate vaccines and biopharmaceutical effects. Here, we developed a two-step model to evaluate the cellular immune response after SARS-CoV-2 infection-induced or spike protein stimulation in peripheral blood mononuclear cells (PBMC) from both unexposed and COVID-19 (primo-infected) individuals (Step1). Moreover, the supernatants of these cultures were ...
Tópico(s): Long-Term Effects of COVID-19
2021 - Multidisciplinary Digital Publishing Institute | Cells