Produção Nacional
Revisado por pares
Beatriz Bellón, Agnès Bégué, Danny Lo Seen, Valentine Lebourgeois, Balbino Antônio Evangelista, Margareth Simões, R. P. D. Ferraz,
Tópico(s): Climate change impacts on agriculture
2018 - Elsevier BV | International Journal of Applied Earth Observation and Geoinformation
Produção Nacional
Revisado por pares
Juliano Daniel Groppo, Sílvia Rafaela Machado Lins, Plínio Barbosa de Camargo, Eduardo Delgado Assad, Hilton Silveira Pinto, Susian Christian Martins, Paulo Salgado, Balbino Antônio Evangelista, E. Vasconcellos, Edson Eyji Sano, E. Pavão, Ramón Gutiérrez Luna, Luiz Antônio Martinelli,
Abstract. In this paper, soil carbon, nitrogen and phosphorus concentrations and stocks were investigated in agricultural and natural areas in 17 plot-level paired sites and in a regional survey encompassing more than 100 pasture soils In the paired sites, elemental soil concentrations and stocks were determined in native vegetation (forests and savannas), pastures and crop–livestock systems (CPSs). Nutrient stocks were calculated for the soil depth intervals 0–10, 0–30, and 0–60 cm for the paired ...
Tópico(s): Soil and Water Nutrient Dynamics
2015 - Copernicus Publications | Biogeosciences
Produção Nacional
Revisado por pares
Eduardo Delgado Assad, Hilton Silveira Pinto, Susian Christian Martins, Juliano Daniel Groppo, Paulo Salgado, Balbino Antônio Evangelista, E. Vasconcellos, Edson Eyji Sano, E. Pavão, Ramón Gutiérrez Luna, Plínio Barbosa de Camargo, Luiz Antônio Martinelli,
Abstract. In this paper we calculated soil carbon stocks in Brazil studying 17 paired sites where soil stocks were determined in native vegetation, pastures and crop-livestock systems (CPS), and in other regional samplings encompassing more than 100 pasture soils, from 6.58 to 31.53° S, involving three major Brazilian biomes: Cerrado, Atlantic Forest, and the Pampa. The average native vegetation soil carbon stocks at 10, 30 and 60 cm soil depth were equal to approximately 29, 64, and 92 Mg ha−1, ...
Tópico(s): Soil Management and Crop Yield
2013 - Copernicus Publications | Biogeosciences
Produção Nacional
Revisado por pares
Saulo Relison Tintino, Veruska Cíntia Alexandrino de Souza, Julia M. A. da Silva, Cícera Datiane de Morais Oliveira‐Tintino, Pedro Silvino Pereira, Tereza Cristina Leal‐Balbino, Antonio Pereira‐Neves, José P. Siqueira-Júnior, José Galberto Martins da Costa, Fabíola Fernandes Galvão Rodrigues, Irwin Rose Alencar de Menezes, Gabriel C. A. da Hora, Maria Carolina P. Lima, Henrique Douglas Melo Coutinho, Valdir de Queiroz Balbino,
Resistance to antibiotics has made diseases that previously healed easily become more difficult to treat. Staphylococcus aureus is an important cause of hospital-acquired infections and multi-drug resistant. NorA efflux pump, present in bacteria S. aureus, is synthesized by the expression of the norA gene. Menadione, also known as vitamin K3, is one of the synthetic forms of vitamin K. Therefore, the aim of this study is to verify the menadione effect on efflux inhibition through NorA pump gene ...
Tópico(s): Vitamin C and Antioxidants Research
2020 - Multidisciplinary Digital Publishing Institute | Membranes
Leticia García-Mochón, Jaime Espín Balbino, Antonio Olry de Labry Lima, Araceli Caro Martínez, Eva Martín Ruiz, Román Pérez Velasco,
To gain knowledge and insights on health technology assessment (HTA) and decision-making processes in Central, Eastern and South Eastern Europe (CESEE) countries. A cross-sectional study was performed. Based on the literature, a questionnaire was developed in a multi-stage process. The questionnaire was arranged according to 5 broad domains: (i) introduction/country settings; (ii) use of HTA in the country; (iii) decision-making process; (iv) implementation of decisions; and (v) HTA and decision-making: ...
Tópico(s): Healthcare Policy and Management
2017 - Elsevier BV | Health Policy
Maria Teresa Montagna, Grazia Lovero, Caterina Coretti, Domenico Martinelli, Osvalda De Giglio, Roberta Iatta, Stella Balbino, Antonio Rosato, Giuseppina Caggiano,
The echinocandins are recommended as first-line therapy for Candida species infections, but drug resistance, especially among Candida glabrata, is becoming more frequent. We investigated the antifungal susceptibility of anidulafungin, caspofungin, and micafungin against 584 isolates of Candida spp. (bloodstream, other sterile sites) collected from patients admitted to an Italian university hospital between 2000 and 2013. The susceptibility was evaluated using the broth microdilution method according ...
Tópico(s): Antimicrobial Resistance in Staphylococcus
2015 - BioMed Central | BMC Microbiology
Produção Nacional
Revisado por pares
C.M. Medeiros do Amaral, Kateřina Cetkovská, Ana PAD Gurgel, Marcus Vinicius Cardoso, Bárbara Simas Chagas, Sérgio SL Paiva Júnior, Rita de Cássia Pereira de Lima, Jacinto da Costa Silva Neto, Luiz AF Silva, Maria TC Muniz, Valdir de Queiroz Balbino, Antônio Carlos de Freitas,
The MDM2 gene is the major negative regulator of p53, a tumor suppressor protein. Single nucleotide polymorphism in promoter region of MDM2 gene leads to increased expression resulting in higher levels of MDM2 protein. This event increases the attenuation of the p53 pathway. Polymorphisms in this gene can interfere in the regulation of cellular proliferation. We evaluated whether MDM2 SNP309 (rs2278744) associated or not with the use of oral contraceptive can heighten susceptibility to development ...
Tópico(s): Endometrial and Cervical Cancer Treatments
2014 - BioMed Central | Infectious Agents and Cancer
Produção Nacional
Revisado por pares
Marcus Vinícius de Aragão Batista, Maria Angélica Ramos da Silva, N.E. Pontes, Marcella Carolina Pinheiro Reis, Annunziata Corteggio, Roberto Soares de Castro, Giuseppe Borzacchiello, Valdir de Queiroz Balbino, Antônio Carlos de Freitas,
Bovine papillomaviruses (BPVs) are a diverse group of double-stranded DNA viruses, of which 12 viral types have been detected and characterized so far. However, there is still a limited understanding of the diversity of BPV. Several putative new BPVs have been detected and some of these have been recently characterized as new viral types. However, only a very limited amount of information is available on the pathology associated with these novel viral types yet this information could be of significant ...
Tópico(s): Veterinary Oncology Research
2013 - Elsevier BV | The Veterinary Journal
Produção Nacional
Revisado por pares
Maria Angélica Ramos da Silva, Marcus Vinícius de Aragão Batista, N.E. Pontes, Erinaldo Ubirajara Damasceno dos Santos, L.C.A. Coutinho, Roberto Soares de Castro, Valdir de Queiroz Balbino, Antônio Carlos de Freitas,
Bovine papillomavirus (BPV) is a diverse group of double-stranded DNA oncogenic viruses, which have been detected in epithelial lesions and body fluids. Most studies of BPV infection rely on a single method for DNA detection; however the use of any single method or technique may underestimate the true prevalence of this virus. The purpose of this study was to compare two PCR strategies for the detection of BPV in skin lesions and fluids: these involve the use of BPV type-specific and consensus primers. ...
Tópico(s): Poxvirus research and outbreaks
2013 - Elsevier BV | Journal of Virological Methods
Produção Nacional
Revisado por pares
C. C. R. Carvalho, Marcus Vinícius de Aragão Batista, Maria Angélica Ramos da Silva, Valdir de Queiroz Balbino, Antônio Carlos de Freitas,
The aim of this study was to evaluate the presence of different types of Bovine papillomavirus (BPV) in cattle skin lesions and to identify new viral types in Brazil. A total of 72 skin lesions were analysed from 66 different bovines by PCR using degenerate and specific primers, and subsequent sequencing. Sequencing quality was determined using Staden package with Phred 30. Similarity analysis was performed with BioEdit and BLAST programs to verify the identity with known BPV types. Phylogenetic ...
Tópico(s): Poxvirus research and outbreaks
2012 - Wiley | Transboundary and Emerging Diseases
Produção Nacional
Revisado por pares
Tiago Albertini Balbino, Antonio A. Malfatti‐Gasperini, Cristiano L. P. Oliveira, Adriano Rodrigues Azzoni, Leide P. Cavalcanti, Lucimara Gaziola de la Torre,
In this study, we characterized the conventional physicochemical properties of the complexes formed by plasmid DNA (pDNA) and cationic liposomes (CL) composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) (50/25/25% molar ratio). We found that these properties are nearly unaffected at the studied ranges when the molar charge ratio (R(±)) between the positive charge from the CL and negative charge from pDNA is ...
Tópico(s): Nanoparticle-Based Drug Delivery
2012 - American Chemical Society | Langmuir
Produção Nacional
Marcos Balbino, Antônio Chaves de Assís Neto, Ricardo Prist, Alice T. Ferreira, Luiz Francisco Poli‐de‐Figueiredo,
Background: Calcium is one of the triggers involved in ischemic neuronal death. Because hypotension is a strong predictor of outcome in traumatic brain injury (TBI), we tested the hypothesis that early fluid resuscitation blunts calcium influx in hemorrhagic shock associated to TBI. Methods: Fifteen ketamine-halothane anesthetized mongrel dogs (18.7 kg ± 1.4 kg) underwent unilateral cryogenic brain injury. Blood was shed in 5 minutes to a target mean arterial pressure of 40 mm Hg to 45 mm Hg and maintained ...
Tópico(s): Cardiac Arrest and Resuscitation
2010 - Lippincott Williams & Wilkins | Journal of Trauma and Acute Care Surgery
Produção Nacional
Revisado por pares
Marco Antônio Balbino, Érica Naomi Oiye, Maria Fernanda Muzetti Ribeiro, José W Cruz Júnior, Izabel Cristina Eleotério, Antônio José Ipólito, Marcelo Firmino de Oliveira,
Tópico(s): Molecular Sensors and Ion Detection
2016 - Springer Science+Business Media | Journal of Solid State Electrochemistry
Produção Nacional
Revisado por pares
Marco Antônio Balbino, Laura Siqueira de Oliveira, Izabel Cristina Eleotério, Érica Naomi Oiye, Maria Fernanda Muzetti Ribeiro, Bruce McCord, Antônio José Ipólito, Marcelo Firmino de Oliveira,
The development of methodologies using inexpensive, fast, and reliable instrumention is desirable in illicit drug analysis. The purpose of this study was based on cyclic voltammetry technique to differentiate the electrochemical behavior of ∆(9) -THC, the psychoactive substance in marijuana, and five different extract plants to yield false positive results after analysis protocol for cannabinoids using thin-layer chromatography and Fast Blue B salt. After applying a deposition potential of -0.5 V in ...
Tópico(s): Neurotransmitter Receptor Influence on Behavior
2016 - Wiley | Journal of Forensic Sciences
Produção Nacional
Revisado por pares
Marco Antônio Balbino, Izabel Cristina Eleotério, Laura Siqueira de Oliveira, Matheus Manoel Teles de Menezes, José Fernando de Andrade, Antônio José Ipólito, Marcelo Firmino de Oliveira,
A maconha é uma das drogas ilícitas mais consumidas no mundo.Sendo assim, os traficantes têm procurado novos meios para mascarar o teste preliminar (colorimétrico) atualmente utilizado.Este trabalho descreve uma metodologia para detecção de delta-9-tetraidrocanabinol (∆ 9 -THC) por voltametria de onda quadrada (SWV) em meio orgânico N,N-dimetilformamida utilizando tetrafluoroborato de tetrabutilamônio (TBATFB) como eletrólito de suporte e eletrodos de trabalho de disco de carbono vítreo e platina.Aplicando- ...
Tópico(s): Electrochemical Analysis and Applications
2014 - Brazilian Chemical Society | Journal of the Brazilian Chemical Society
Produção Nacional
Revisado por pares
Érica Naomi Oiye, Maria Fernanda Muzetti Ribeiro, Juliana Midori Toia Katayama, Maraine Catarina Tadini, Marco Antônio Balbino, Izabel Cristina Eleotério, Juliana Gallottini Magalhães, Alex Soares Castro, Ricardo Soares Mota Silva, José Wilmo da Cruz Júnior, Edward R. Dockal, Marcelo Firmino de Oliveira,
Schiff bases and their transition metal complexes are inexpensive and easy to synthesize. These compounds display several structural and electronic features that allow their application in numerous research fields. Over the last three decades, electroanalytical scientists of various areas have developed electrochemical sensors from many compounds. The present review discusses the applicability of Schiff bases, their transition metal complexes and new materials containing these compounds as electrode ...
Tópico(s): Molecular Sensors and Ion Detection
2019 - Taylor & Francis | Critical Reviews in Analytical Chemistry
Produção Nacional
Revisado por pares
Maraine Catarina Tadini, Marco Antônio Balbino, Izabel Cristina Eleotério, Laura Siqueira de Oliveira, Luís Gustavo Dias, Grégoire Jean‐François Demets, Marcelo Firmino de Oliveira,
This study aimed to develop an electrode chemically modified with cucurbit[6]uril to detect 3,4-methylenedioxymethamphetamine (MDMA), the main active principle of ecstasy samples, by voltammetry. We modified the electrode surface with a film containing cucurbit[6]uril, Nafion, and methanol, using the dip coating or the spin coating technique. During analysis, we employed an electrochemical cell with a conventional three-electrode system and KCl solution (0.1 mol L−1) as the supporting electrolyte. ...
Tópico(s): Supramolecular Chemistry and Complexes
2014 - Elsevier BV | Electrochimica Acta
Produção Nacional
Revisado por pares
Márcia Cristina Teixeira Ribeiro Vidigal, Valéria Paula Rodrigues Minim, Andréa Alves Simiqueli, Pedro H.P. Souza, Douglas Fernando Balbino, Luís Antônio Minim,
New food technologies are promoting innovations in the food sector. However, not all technologies are accepted and understood by consumers; some cause resistance. The present work sought to study the behavior of Brazilian consumers in relation to different food technologies. A questionnaire was administered to a representative sample of 389 respondents in Belo Horizonte-MG, Brazil. Questionnaire collected information on consumer perceptions regarding new technologies by means of the Food Technology ...
Tópico(s): Consumer Packaging Perceptions and Trends
2014 - Elsevier BV | LWT
Produção Nacional
Revisado por pares
Laura de Oliveira, Ana Dos Santos Poles, Marco Antônio Balbino, Matheus Teles de Menezes, José de Andrade, Edward R. Dockal, Heloísa Maria Tristão, Marcelo Firmino de Oliveira,
A fast and non-destructive voltammetric method to detect cocaine in confiscated samples based on carbon paste electrode modified with methoxy-substituted N,N'-ethylene-bis(salcylideneiminato)uranyl(VI)complexes, [UO2(X-MeOSalen)(H2O)]·H2O, where X corresponds to the positions 3, 4 or 5 of the methoxy group on the aromatic ring, is described. The electrochemical behavior of the modified electrode and the electrochemical detection of cocaine were investigated using cyclic voltammetry. Using 0.1 mol· ...
Tópico(s): Analytical Methods in Pharmaceuticals
2013 - Multidisciplinary Digital Publishing Institute | Sensors
Produção Nacional
Revisado por pares
Laura Siqueira de Oliveira, Marco Antônio Balbino, Matheus Manoel Teles de Menezes, Edward R. Dockal, Marcelo Firmino de Oliveira,
Platinum and glassy carbon electrodes chemically modified with films of Schiff bases of [UO2(3-MeOSalen)(H2O)] · H2O and [UO2(5-MeOSalen)(H2O)] · H2O, to determine cocaine were developed. The stability of these films in aqueous solution containing 1.0 mol L− 1 KCl and HCl 1.0 mM in pH 3 as supporting electrolyte and conducted cyclic voltammetric analysis of cocaine in the same supporting electrolyte were investigated. A reversible interaction between cocaine and the working electrodes increased the original ...
Tópico(s): Electrochemical Analysis and Applications
2013 - Elsevier BV | Microchemical Journal
Produção Nacional
Revisado por pares
Marco Antônio Balbino, Matheus Manoel Teles de Menezes, Izabel Cristina Eleotério, Adelir Aparecida Saczk, Leonardo Luiz Okumura, Heloísa Maria Tristão, Marcelo Firmino de Oliveira,
A new voltammetric method for the determination of Δ9-tetrahydrocannabinol (Δ9-THC) is described. The voltammetric experiments were accomplished in N-N dimethylformamide/water (9:1, v/v), using tetrabutylammonium tetrafluoroborate (TBATFB) 0.1 mol/L as supporting electrolyte and a glassy carbon disk electrode as the working electrode. The anodic peak current was observed at 0.0 V (vs. Ag/AgCl) after a 30 s pre-concentration step under an applied potential of −1.2 V (vs. Ag/AgCl). A linear dependence of ...
Tópico(s): Molecular Sensors and Ion Detection
2012 - Elsevier BV | Forensic Science International
Gemma Fernández-Miguel, Balbino Alarcón, Antonio Iglesias, Horst Bluethmann, Melchor Álvarez‐Mon, Eva Sanz, Antonio de la Hera,
Whether there is one or multiple αβT cell antigen receptor (TCR) recognition modules in a given TCR/CD3 complex is a long-standing controversy in immunology. We show that T cells from transgenic mice that coexpress comparable amounts of two distinct TCRβ chains incorporate at least two αβTCRs in a single TCR/CD3 complex. Evidence for bispecific αβTCRs was obtained by immunoprecipitation and immunoblotting and confirmed on the surface of living cells both by fluorescence resonance energy transfer ...
Tópico(s): Immunotherapy and Immune Responses
1999 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Pedro Bonay, Isabel Durán-Chica, Manuel Fresno, Balbino Alarcón, Antonio Alcina,
ABSTRACT The macrolide antibiotic megalomicin (MGM) has been shown to inhibit vesicular transport between the medial- and trans-Golgi, resulting in the undersialylation of cellular proteins (P. Bonay, S. Munro, M. Fresno, and B. Alarcón, J. Biol. Chem. 271:3719–3726, 1996). Due to the effects of MGM on the Golgi and on the replication of enveloped viruses, we decided to test whether it has any antiparasitic activity. The results showed that MGM has potent activity against the epimastigote stage of ...
Tópico(s): Complement system in diseases
1998 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Paloma Pérez‐Aciego, Balbino Alarcón, Antonio Arnaiz‐Villena, Cox Terhorst, Marcos Timón, Oscar G. Segurado, José R. Regueiro,
A T cell line termed DIL2 has been derived from an infant with a polyclonal T cell receptor (TCR)/CD3 cell surface expression defect. Indirect immunofluorescence showed that the expression of certain TCR/CD3 epitopes (like those detected by WT31 and BMA031 monoclonals) was strongly reduced (around five-fold) on DIL2, whereas other epitopes (like those detected by SP34 and Leu4) were only around two-fold lower than in normal T cell lines. Specific immunoprecipitates of surface-radioiodinated DIL2 cells ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
1991 - Rockefeller University Press | The Journal of Experimental Medicine
Produção Nacional
Revisado por pares
P. M. Santos, José Ricardo Macedo Pezzopane, Fernando Campos Mendonça, G. M. Bettiol, Balbino Antônio Evangelista, Fernando Antônio Macena da Silva,
Climatic risk zoning allows the identification of areas of low risk of reduced crop productivity due to climatic events. In Brazil, losses to agriculture due to climate are mainly caused by drought. The objective of this research was to determine areas of low climatic risk for corn and palisade grass cultivated in integrated crop-livestock systems in Sao Paulo state. Corn varieties characterized by a 120-day growing cycle and soil with three different water holding capacities (sandy, medium and clayey ...
Tópico(s): Growth and nutrition in plants
2012 - Sociedade Brasileira de Zootecnia | Revista Brasileira de Zootecnia
M. Grandé, Maria J. Macías, Balbino Mancheño, Miguel Segura, Antonio Zarzo,
... Parts of Distichoselinum tenuifoliumManuel Grande, María Jesús Macías, Balbino Mancheño, Miguel Segura, and Antonio ZarzoCite this: J. Nat. Prod. 1991, 54, 3, ...
Tópico(s): Advanced Synthetic Organic Chemistry
1991 - American Chemical Society | Journal of Natural Products
Produção Nacional
Revisado por pares
Michele Lima Gregório, Marcela Augusta Souza Pinhel, Caroline L. Sado, G. Longo, Fábio N. Oliveira, Gisele S. Amorim, Marcelo Arruda Nakazone, Greiciane Maria da Silva Florim, Camila Montoro Mazeti, Denise Poltronieri Martins, Waldir Antônio Tognola, Antônio Carlos Balbino Brandão, Sidney Pinheiro Júnior, Moacir Fernandes de Godoy, Dorotéia Rossi Silva Souza,
The pathogenesis of Parkinson's disease (PD) seems to involve genetic susceptibility to neurodegeneration. APOE gene has been considered a risk factor for PD. This study aimed to evaluate the association of APOE polymorphism with PD and its influence on lipid profile. We studied 232 PD patients (PD) and 169 individuals without the disease. The studied polymorphism was analyzed by PCR/RFLP. The Fisher's exact test, chi-square, ANOVA, and t-test (P < 0.05) were applied. The APOE3/3 genotype was prevalent ...
Tópico(s): Neurological diseases and metabolism
2013 - Hindawi Publishing Corporation | BioMed Research International
Produção Nacional
Revisado por pares
Bárbara Simas Chagas, Marcus Vinícius de Aragão Batista, Vilma Guimarães, Valdir de Queiroz Balbino, Sérgio Crovella, Antônio Carlos de Freitas,
We sought to characterize E6 and E7 oncogenes genetic variability of HPV-31 isolated from cervical scraping samples of Northeastern Brazilian women.E6 and E7 were amplified with specific primers, cloned and sequenced. The sequences obtained were aligned with the GenBank reference sequences with the aim of evaluating the possible genetic variants.We identified several genetic variants in E6 and E7 sequences from HPV-31 positive women. Three nucleotide changes in E6 were described for the first time ...
Tópico(s): Hepatitis B Virus Studies
2011 - Elsevier BV | Gynecologic Oncology
Produção Nacional
Revisado por pares
Karla Pereira Balbino, Antonio Epifanio, Sônia Machado Rocha Ribeiro, L. D. M. da Silva, Marcia Gouvea, Helen Hermana Miranda Hermsdorff,
Abstract Background The present study aimed to evaluate the nutritional status of patients undergoing haemodialysis ( HD ) by comparing nutritional risk scores with biochemical, anthropometric and body composition variables. Methods Eighty‐five individuals [65.9% male, mean (SD) age 62 (14) years] participated in a cross‐sectional study. Global Objective Assessment ( GOA ) and Modified Global Subjective Assessment ( mGSA ) scores, as well as biochemical, anthropometric and body composition data, were collected ...
Tópico(s): Iron Metabolism and Disorders
2017 - Wiley | Journal of Human Nutrition and Dietetics
Produção Nacional
Revisado por pares
Tiago Albertini Balbino, Juliana M. Serafin, Antonio A. Malfatti‐Gasperini, Cristiano L. P. Oliveira, Leide P. Cavalcanti, Marcelo Bispo de Jesus, Lucimara Gaziola de la Torre,
Microfluidics offers unique characteristics to control the mixing of liquids under laminar flow. Its use for the assembly of lipoplexes represents an attractive alternative for the translation of gene delivery studies into clinical trials on a sufficient throughput scale. Here, it was shown that the microfluidic assembly of pDNA/cationic liposome (CL) lipoplexes allows the formation of nanocarriers with enhanced transfection efficiencies compared with the conventional bulk-mixing (BM) process under ...
Tópico(s): Advanced biosensing and bioanalysis techniques
2016 - American Chemical Society | Langmuir