... MLA80 rotor for 10 min. 200 μl of anti-FLAG M2 affinity resin (Sigma) was added to 10 ... extract mix was rotated for 3 h. The anti-FLAG resins were then washed with 15 bead volumes ... 30 min and added to ∼100 μl of anti-FLAG M2 resins and IgG resins (IgG-Sepharose 6 ... the supernatant was added to 20 μl of anti-FLAG M2 resin for 2 h. The anti-FLAG M2 column was washed, and Dun1 was eluted ... B and then incubated with 3 ml of anti-FLAG M2 resin for 4 h. After washing, proteins ... described for the purification of recombinant Mrc1, except anti-FLAG resins were used during the second step of ...
Tópico(s): Microtubule and mitosis dynamics
2009 - Elsevier BV | Journal of Biological Chemistry
Holly McDonough, Peter C. Charles, Eleanor Hilliard, Shu Bing Qian, Jin-Na Min, Andrea L. Portbury, Douglas Cyr, Cam Patterson,
... with the appropriate dilutions of the primary antibodies: anti-FLAG (Sigma), anti-Myc (Santa Cruz Biotechnology), anti-Daxx ( ... min. The cell lysates were immunoprecipitated (IP) with anti-FLAG resin for immunoprecipitating Daxx, or conversely, immunoprecipitated with ... control and probed for Daxx and CHIP with anti-FLAG and anti-Myc respectively. B, COS-7 cells ... n) fractions. These fractions were then immunoprecipitated with anti-FLAG resin for Daxx and immunoblotted with anti-FLAG for Daxx and anti-Myc for CHIP. Immunoblotting ...
Tópico(s): Ubiquitin and proteasome pathways
2009 - Elsevier BV | Journal of Biological Chemistry
Akinori Endo, Naomi Kitamura, Masayuki Komada,
... μg; Zymed Laboratories Inc., South San Francisco, CA), anti-FLAG (1 μg; clone M2, Sigma), and anti-GFP ( ... 57) Google Scholar), anti-NPM (2 μg/ml), anti-FLAG (4 μg/ml), anti-GFP (4 μg/ml), ... secondary antibodies. The primary antibodies used were rabbit anti-FLAG (0.4 μg/ml), mouse anti-nucleolus (1: ... not shown). Immunofluorescence staining of transfected cells with anti-FLAG antibody showed that the mutants lacking each of ... with FLAG-tagged USP36 mutants were immunoprecipitated with anti-FLAG antibody and immunoblotted with anti-NPM (upper panel) ...
Tópico(s): Genetics and Neurodevelopmental Disorders
2009 - Elsevier BV | Journal of Biological Chemistry
Saulius Vainauskas, Yusuke Maeda, Henry Kurniawan, Taroh Kinoshita, Anant K. Menon,
... D6 and D7. Immunoblots were carried out with anti-FLAG antibodies (top panel), anti-Gpi8 antibodies (middle panel), ... D12 was monitored by immunoprecipitation and immunoblotting with anti-FLAG antibodies as in Fig. 7. The band marked ... C. To each supernatant fraction, 30 μl of anti-FLAG M2 agarose (Sigma) slurry was added, and the ... mm dithiothreitol. Bound antigen was released from the anti-FLAG M2 agarose beads by incubation with FLAG peptide ( ... incubated for 1 h at room temperature with anti-FLAG monoclonal antibody at 1 μg/ml, anti-V5 ...
Tópico(s): Cellular transport and secretion
2002 - Elsevier BV | Journal of Biological Chemistry
Masahiko Kishimoto, Yasuhiko Okimura, Kazuhiro Yagita, Genzo Iguchi, Mariko Fumoto, Keiji Iida, Hidesuke Kaji, Hitoshi Okamura, Kazuo Chihara,
... HA antibody and the other for immunoprecipitation with anti-FLAG M2 monoclonal antibody (Sigma). These samples were then resolved by SDS-PAGE and anti-FLAG M2 monoclonal antibody was used for Western blotting. ... HA antibody and the other for immunoprecipitation with anti-FLAG antibody and both were analyzed by Western blotting with anti-FLAG antibody (Fig. 4 B). pPit-1-FLAG, pP14L- ... transfected (Fig. 4B, lanes 6–10; immunoprecipitated with anti-FLAG antibody and Western blotted with anti-FLAG antibody). ...
Tópico(s): Genetic Syndromes and Imprinting
2002 - Elsevier BV | Journal of Biological Chemistry
Kota Saito, Jun Murai, Hiroaki Kajiho, Kenji Kontani, Hiroshi Kurosu, Toshiaki Katada,
... effector for Rab5 in the endocytic pathway. Monoclonal anti-FLAG(M2) and anti-Myc (9E10) antibodies were purchased ... been conjugated with 0.5 μg of the anti-FLAG monoclonal antibody. The resin was washed three times ... and 15%, respectively. Immunoblotting was performed with the anti-FLAG monoclonal antibody and the anti-Rab5b polyclonal antibody ... Sepharose 4B resins (Amersham Biosciences) and immunoprecipitated with anti-FLAG M2-agarose beads (Sigma) or anti-Myc 9E10 ( ... by SDS-PAGE, and immunoblotting was performed with anti-FLAG and anti-Myc antibodies. For gel filtration analysis, ...
Tópico(s): Calcium signaling and nucleotide metabolism
2002 - Elsevier BV | Journal of Biological Chemistry
... BARF0 gene. BARF0 was detected by immunoblotting with anti-FLAG antibody. (C) EBER expression. EBER and GAPDH expression ... FLAG-PKR in cell lysates was immunoprecipitated with anti-FLAG M2 antibody, labeled with [γ-32P]ATP and ... h of incubation, FLAG-PKR was immunoprecipitated with anti-FLAG antibody and subjected to in vitro kinase assay. Immunoprecipitated FLAG-PKR was detected by immunoblotting using anti-FLAG antibody (lower panel), and its phosphorylation was visualized ... the FLAG-tagged PKR plasmid, precipitated with the anti-FLAG antibody, and determined by RT–PCR whether EBERs ...
Tópico(s): Viral-associated cancers and disorders
2002 - Springer Nature | The EMBO Journal
... 6, 11 and 12) were coimmuno precipated with anti-Flag-agarose (lanes 1–4, respectively). RBP2N was only ... 6, 11 and 12 were used in an anti-Flag coimmunoprecitation (Figure 2C). The RBP protein displaced from ... indicate the heavy and light chains of the anti-Flag antibody. Download figure Download PowerPoint In vivo interaction ... of TNT–SHARP proteins was controlled in the anti-Flag western blot shown in Figure 4A (WB). The ... lanes 3, 6 and 9). Addition of an anti-Flag antibody had no effect on the RBP DNA- ...
Tópico(s): Wnt/β-catenin signaling in development and cancer
2002 - Springer Nature | The EMBO Journal
Hitoshi Nochi, Naoko Aoki, Kensuke Oikawa, Mitsuru Yanai, Yumi Takiyama, Yoshiaki Atsuta, Hiroya Kobayashi, Keisuke Sato, Masatoshi Tateno, Takeo Matsuno, Makoto Katagiri, Zhou Xing, Shoji Kimura,
... enhanced granuloma formation beyond day 7. However, an anti-FLAG monoclonal antibody used to potentiate the signaling of ... enhanced granuloma formation beyond day 7. However, an anti-FLAG monoclonal antibody used to potentiate the signaling of ... was purchased from BMA Biomedicals (August, Switzerland). Mouse anti-FLAG mAb (M2) was purchased from Sigma (St. Louis, ... and probed with rabbit anti-DAP12 Ab or anti-FLAG mAb (M2) (Sigma) followed by donkey anti-rabbit ... Nunc International Corp., Naperville, IL.) were incubated with anti-FLAG mAb (M2) (20 μg/ml in PBS) overnight ...
Tópico(s): Immune Cell Function and Interaction
2003 - Elsevier BV | American Journal Of Pathology
Wu Xiang Liao, R. Kelly Moore, Fumio Otsuka, Shunichi Shimasaki,
... zeocin were purchased from Invitrogen (San Diego, CA). Anti-FLAG M2 monoclonal antibody (anti-FLAG mAb), Anti-FLAG M2 affinity gel, and Triton X-100 were ... to SDS/PAGE-immunoblotting using 12% polyacrylamide gels. Anti-FLAG Ab were used to detect BMP-15, and ... on ice followed by SDS/PAGE immunoblotting analysis. Anti-FLAG monoclonal antibodies conjugated to agarose beads (Anti-FLAG M2 affinity gel, 10 μl) and Triton X- ... subjected to SDS/PAGE immunoblotting analysis using an anti-FLAG Ab to detect BMP-15 and an anti- ...
Tópico(s): Renal and related cancers
2003 - Elsevier BV | Journal of Biological Chemistry
Yong Cai, Jingji Jin, Chieri Tomomori‐Sato, Shigeo Sato, Irina Sorokina, Tari Parmely, Ronald Conaway, Joan Conaway,
... components of the TRRAP/TIP60 HAT complex. Materials—Anti-FLAG-agarose, anti-FLAG (M2) monoclonal anti-bodies, and anti-FLAG peptide were purchased from Sigma. Anti-TRRAP (T- ... FLAG-MRGBP expressing HeLa cells and subjected to anti-FLAG-agarose immunoaffinity chromatography. As shown in the silver-stained SDS-polyacrylamide gel of Fig. 1A, anti-FLAG-agarose eluates from FLAG-MRGBP expressing HeLa cells ... FLAG-MRGBP, since they are not apparent in anti-FLAG-agarose eluates prepared from parental HeLa cells. To ...
Tópico(s): Peptidase Inhibition and Analysis
2003 - Elsevier BV | Journal of Biological Chemistry
Akira Matsuda, Yoshifumi Itoh, Naohiko Koshikawa, Toshifumi Akizawa, Ikuo Yana, Motoharu Seiki,
... DMEM), RPMI 1640 medium, FLAG peptide, monoclonal mouse anti-FLAG M2 antibody, and anti-FLAG M2-agarose were purchased from Sigma. Monoclonal mouse ... 35. This fraction was then applied to an anti-FLAG M2-agarose column, and the column was washed ... for 15 min. The supernatant was incubated with anti-FLAG M2-agarose (Sigma) at 4 °C for 6 ... FLAG peptide and analyzed by Western blotting using anti-FLAG M2 antibody or anti-human clusterin antibody. Other ... reducing conditions as demonstrated by Western blotting using anti-FLAG M2 monoclonal antibody (anti-FLAG mAb). sMT6-F ...
Tópico(s): Protease and Inhibitor Mechanisms
2003 - Elsevier BV | Journal of Biological Chemistry
Cheng Cao, Yumei Leng, Chufang Li, Donald Küfe,
... subjected to immunoprecipitation with anti-Myc (Santa Cruz), anti-FLAG (M5, Sigma), anti-c-Abl (24–11, Santa ... Invitrogen). Immunoblot analysis was performed with anti-Myc, anti-FLAG, anti-Arg (38Cao C. Ren X. Kharbanda S. ... lysis buffer and then subjected to immunoblotting with anti-FLAG. An aliquot of the total lysate (2% v/ ... expressing FLAG-Arg were subjected to immunoprecipitation with anti-FLAG. The protein complexes were washed, normalized by immunoblotting, ... Arg). Immunoblot analysis of anti-Myc immunoprecipitates with anti-FLAG demonstrated detection of complexes containing c-Abl and ...
Tópico(s): Fungal Plant Pathogen Control
2003 - Elsevier BV | Journal of Biological Chemistry
... western blotting analysis to detect bound Stat3 using anti-Flag antibody. As shown in Figure 1, Stat3 was ... lysates were immunoprecipitated with either anti-Myc or anti-Flag as indicated. The precipitates were fractionated by 12.5% SDS–PAGE, and blotted with anti-Flag (top left panel) or anti-Myc (top right ... stripped and re-probed with anti-Myc or anti-Flag (middle panels). Total cell lysates (TCL) were subjected to western blot analysis with anti-Flag or anti-Myc to monitor the expression of ... proteins were subjected to western blot analysis with anti-Flag antibody (top panel). Precipitated GST and GST–GRIM- ...
Tópico(s): interferon and immune responses
2003 - Springer Nature | The EMBO Journal
... with 1 μg/ml LPS for 20 min. Anti-Flag and anti-Myc immunoprecipitates were separated by SDS–PAGE and immunoblotting was performed with anti-Flag (upper) and anti-Myc (lower) antibodies. (C) HEK ... 48 h after transfection, cell lysates were prepared. Anti-Flag and control antibody immunoprecipitates were separated by SDS– ... top panel). Cell lysates were also probed with anti-Flag or anti-GCSFR antibody. (D) RAW264.7 cells ... antibody and coprecipitation of TLR4 was detected with anti-Flag antibody. Lysates were also run as controls for ...
Tópico(s): interferon and immune responses
2003 - Springer Nature | The EMBO Journal
Dylan Sweetman, Terry Smith, Elizabeth R. Farrell, Andrew Chantry, Andrea Münsterberg,
... μl of either anti-HA.11 (Babco) or anti FLAG M2 (Sigma) and shaken for 1 h at ... with primary antibodies, anti-HA.11 (Babco) or anti-FLAG polyclonal (Sigma), and anti mouse-FITC and anti ... FLAG were immunoprecipitated with both anti-HA and anti-FLAG antibodies. In neither case did we observe nonspecific ... found that immunoprecipitation of csal1-FLAG with an anti-FLAG antibody resulted in coprecipitation of csal1-HA-tagged ... 6). Similarly, immunoprecipitation of csal3-FLAG with an anti-FLAG antibody resulted in coprecipitation of csal3-HA-tagged ...
Tópico(s): Animal Genetics and Reproduction
2003 - Elsevier BV | Journal of Biological Chemistry
Marta Busse, Marion Kusche‐Gullberg,
... of purified enzymes. Recombinant proteins were captured on anti-FLAG agarose (Sigma) during overnight incubation at 4 °C, ... polyvinylidene difluoride membrane (Millipore). The antibodies used were anti-FLAG M2 monoclonal antibody (Sigma) detected using horseradish peroxidase- ... Full Text PDF PubMed Google Scholar) by incubating anti-FLAG agarose-bound enzyme protein preparations with 0.125 ... the complex was affinity adsorbed from medium onto anti-FLAG agarose and visualized by Western blotting with anti- ... 2; Fig. 1) that could be captured on anti-FLAG agarose (data not shown, but see below). Soluble ...
Tópico(s): Connective tissue disorders research
2003 - Elsevier BV | Journal of Biological Chemistry
Tohru Ishitani, Giichi Takaesu, Jun Ninomiya‐Tsuji, Hiroshi Shibuyà, Richard B. Gaynor, Kunihiro Matsumoto,
... immunoprecipitated with anti-HA antibody were immunoblotted with anti-Flag, anti-T7 or anti-HA antibodies. Whole-cell extracts were immunoblotted with anti-Flag or anti-T7 antibodies. (E) Interaction among TAK1- ... The immune complexes were subjected to immunoblotting with anti-Flag antibody. As observed previously, TAB2 was found to ... immunoprecipitated with anti-T7 antibody were immunoblotted with anti-Flag or anti-T7 antibodies. Whole-cell extracts were immunoblotted with anti-Flag antibody. (C) Effect of TAB2 on the interaction ...
Tópico(s): interferon and immune responses
2003 - Springer Nature | The EMBO Journal
Christine McDonald, Panayiotis O. Vacratsis, James B. Bliska, Jack E. Dixon,
... following antibodies were used in the experiments described: anti-FLAG M2 (Sigma), anti-PRK2 (Cell Signaling Technology and ... were then incubated with 15–20 μl of anti-FLAG M2-Sepharose (Sigma) or 1–2 μg of ... or the empty FLAG vector were immunoprecipitated with anti-FLAG antibody, and the associated proteins were visualized by ... YopM plasmid or vector alone were immunoprecipitated with anti-FLAG antibody, and proteins were visualized by sequential Western blots using anti-FLAG, anti-PRK2, and anti-RSK1 antibodies. A single ∼ ...
Tópico(s): Pharmacological Effects of Natural Compounds
2003 - Elsevier BV | Journal of Biological Chemistry
... acid-agarose was purchased from Qiagen (Hilden, Germany). Anti-FLAG M2 antibody and anti-FLAG M2 affinity gel were from Sigma. FLAG peptide ( ... loaded on a column containing 1 ml of anti-FLAG M2 affinity gel pre-equilibrated with solution A. ... Only WT(F)/ST(H) bound to the anti-FLAG M2-agarose gel and was eluted by FLAG ... by a single affinity chromatography, Ni2+-agarose, or anti-FLAG M2 affinity gel, respectively. Mutant homodimer HMM (i. ... of affinity chromatography, Ni2+-agarose affinity chromatography, and anti-FLAG M2 affinity chromatography. The extract was applied to ...
Tópico(s): Cardiovascular Effects of Exercise
2003 - Elsevier BV | Journal of Biological Chemistry
Martin A. Hoyt, Mingsheng Zhang, Philip Coffino,
... stability in yeast cells. A, immunoblot analysis with anti-FLAG antibody of spe1Δspe2Δ cells transformed with p414ADH vectors ... or left untreated, and FLAGmODC was immunoprecipitated with anti-FLAG affinity gel.View Large Image Figure ViewerDownload (PPT) ... degradation in yeast cells. A, immunoblot analysis with anti-FLAG antibody of spe1Δspe2Δ cells transformed with a p424ADH ... as a loading control. B, immunoblot analysis with anti-FLAG antibody ofspe1Δspe2Δ cells transformed with a p414ADH vector ... expressed from p416GPD. C, pulse-chase analysis using anti-FLAG affinity gel for immunopurification of labeled FLAGmODC from ...
Tópico(s): Epigenetics and DNA Methylation
2003 - Elsevier BV | Journal of Biological Chemistry
Stewart T. Moran, K Haider, Yongkai Ow, Peter Milton, Luojing Chen, Shiv Pillai,
... and the post-mitochondrial supernatant was immunoprecipitated using anti-FLAG (M2 monoclonal; Sigma) antibodies. Equal amounts of each immunoprecipitate were analyzed using an anti-FLAG immunoblot assay and an in vitro kinase assay. ... from these transient transfections were then immunoprecipitated with anti-FLAG antibodies. Half of each immunoprecipitate was used in ... substrate; the other half was examined using an anti-FLAG immunoblot assay (to assess protein levels). The catalytic ... the decreased mobility of K51R-PKK on an anti-FLAG Western blot (Fig. 2A and data not shown). ...
Tópico(s): Signaling Pathways in Disease
2003 - Elsevier BV | Journal of Biological Chemistry
Ruth F. Knight, David M. Bader, Jon R. Backstrom,
... to compare the immunoreactivity of anti-HA and anti-FLAG antibodies. An insert (GVEVEAVSLLNQTV), based on a glycosylated ... Development. 2001; 128: 2085-2093PubMed Google Scholar). Monoclonal anti-FLAG (M2; 1:1000), polyclonal anti-HA (1:250), ... the manufacturer's protocol. Protein was immunoprecipitated with anti-FLAG and subjected to electrophoresis under reducing conditions. Separate lanes were probed with either anti-FLAG to detect total cellular protein or with 0. ... of either Met128-FLAG or 2C/Met128-FLAG. Anti-FLAG (2 μg) was added to tubes containing 1% ...
Tópico(s): Renal and related cancers
2003 - Elsevier BV | Journal of Biological Chemistry
Anamarija Kruljac‐Letunic, Jörg Moelleken, Anders Kallin, Felix Wieland, Andree Blaukat,
... from Schleicher & Schuell; epidermal growth factor, GTP, GTPγS, anti-FLAG M2 affinity agarose, anti-FLAG (M2) monoclonal antibody, and phosphoamino acid standards were ... PDF PubMed Scopus (115) Google Scholar)) or an anti-FLAG monoclonal antibody to detect FLAG-tagged ASAP1 constructs. ... Text PDF PubMed Scopus (115) Google Scholar)) or anti-FLAG antibodies. Immune complexes were isolated using protein A- ... from transiently transfected HEK293T cells, affinity purification with anti-FLAG M2 affinity matrix following the manufacturer's instructions ( ...
Tópico(s): Wnt/β-catenin signaling in development and cancer
2003 - Elsevier BV | Journal of Biological Chemistry
Abhinav K. Jain, Shilpi Mahajan, Anil K. Jaiswal,
... immunoblotting with anti-V5HRP, anti-Tyr(P), or anti-FLAG-HRP antibodies. FLAG immunoprecipitation was done using the anti-FLAG-M2 agarose beads (Sigma). In related experiments, 1. ... binding experiment, the protein mixtures were immunoprecipitated with anti-FLAG-M2 beads (Sigma). Finally, the beads were boiled ... lysed and immunoprecipitated with either anti-V5 or anti-FLAG antibodies. The immunoprecipitate was analyzed by immunoblotting with anti-V5 and anti-FLAG antibodies. Anti-V5 antibody immunoprecipitated INrf2-V5 and ...
Tópico(s): Health, psychology, and well-being
2008 - Elsevier BV | Journal of Biological Chemistry
Akiko Mukai, Miki Yamamoto‐Hino, Wakae Awano, Wakako Watanabe, Masayuki Komada, Satoshi Goto,
... FLAG-Fz4 was immunoprecipitated from the lysates with anti-FLAG antibody and immunoblotted with anti-Ub antibody. Fz4 ... HA-tagged Fz4, ubiquitylated Fz4 was detected by anti-FLAG immunoblotting of anti-HA immunoprecipitate (Figure 3C, left, ... expressed in COS-7 cells and immunopurified with anti-FLAG beads, were stained with Coomassie Brilliant Blue (CBB) ... with biotin and sequentially precipitated with streptavidin and anti-FLAG antibody. Immunoblotting of precipitated FLAG-Fz4K0 with anti- ... UBPY proteins were immunopurified from transfected cells using anti-FLAG antibody (Figure 3G, left) and incubated with ubiquitylated ...
Tópico(s): Developmental Biology and Gene Regulation
2010 - Springer Nature | The EMBO Journal
M Moretto Zita, Ivan Marchionni, Elisa Bottos, Massimo Righi, Giannino Del Sal, Enrico Cherubini, Paola Zacchi,
... kDa molecular weight was recognised only in the anti-FLAG immunoprecipitate obtained from CIP-untreated cell lysate (Figure ... lysate was treated with CIP and immunoprecipitated with anti-FLAG monoclonal antibody (lanes 4 and 6) and with ... on SDS-containing gels and immunoblotted using the anti-FLAG antibody. As shown in Figure 2A, only GST- ... 3) pull-down followed by Western blot with anti-FLAG antibody. (B) Endogenous gephyrin from lysates of a ... fluorescence of GFP. Pin1-FLAG was visualised by anti-FLAG antibody, followed by TRITC-conjugated secondary antibody. Scale ...
Tópico(s): Signaling Pathways in Disease
2007 - Springer Nature | The EMBO Journal
Isao Kuraoka, Shinsuke Ito, Tadashi Wada, Mika Hayashida, Lily Lee, Masafumi Saijo, Yoshimichi Nakatsu, Megumi Matsumoto, Tsukasa Matsunaga, Hiroshi Handa, Jun Qin, Yoshihiro Nakatani, Kiyoji Tanaka,
... HeLa cells expressing FLAG-XAB2 fusion protein using anti-FLAG antibody beads. The XAB2 complex consists of hAquarius ( ... FLAG-XAB2 complexes were purified by immunoprecipitation with anti-FLAG antibody from cell extracts. FLAG-XAB2-containing fractions ... affinity-purified using a protein A-Sepharose column. Anti-FLAG monoclonal antibody (M2) was purchased from Sigma. Anti- ... 108) Google Scholar). The extracts were incubated with anti-FLAG antibody (M2)-conjugated agarose (Sigma) and rotated at ... FLAG-XAB2 proteins were detected by immunoblotting using anti-FLAG antibody (M2). The band indicated with an asterisk ...
Tópico(s): CRISPR and Genetic Engineering
2007 - Elsevier BV | Journal of Biological Chemistry
... Bjorkman, California Institute of Technology). Generation of rabbit anti-FLAG, monoclonal antibodies 3B8 2A1 against the ectodomain of ... specific to the cytoplasmic domain of TfR1, M2 anti-FLAG, mouse monoclonal anti-actin, and rabbit anti-ferritin ... and Western blot analysis and immunofluorescence with M2 anti-FLAG antibody. Transfected cells were maintained in minimal essential ... transfer to nitrocellulose, proteins were detected using M2 anti-FLAG (1:4,000), rabbit anti-TfR2 (1:10, ... secondary antibody. All mouse monoclonal primary antibodies (M2 anti-FLAG, anti-actin, 3B8 2A1, and H68.4) were ...
Tópico(s): Trace Elements in Health
2007 - Elsevier BV | Journal of Biological Chemistry
Chao Liu, Erkang Fei, Nali Jia, Hongfeng Wang, Rui-Song Tao, Atsushi Iwata, Nobuyuki Nukina, Jiang‐Ning Zhou, Guanghui Wang,
... for 1 h, the cells were incubated with anti-FLAG antibody (1:5000, Sigma) for 1 h at ... monoclonal anti-V5 antibody (1:500, Invitrogen), monoclonal anti-FLAG antibody (1:5000, Sigma), and monoclonal anti-α- ... immunofluorescence. Immunoblotting analysis revealed the smear bands of anti-FLAG immunoreactivity that represented the Ub-conjugates. In Nonidet ... excitation of EGFP and immunochemistry analysis using the anti-FLAG antibody. As shown in Fig. 2, C-K, ... FLAG-Ub was visualized by immunocytochemistry analysis using anti-FLAG anti-body. There are different patterns of α- ...
Tópico(s): Nuclear Receptors and Signaling
2007 - Elsevier BV | Journal of Biological Chemistry