Liam Collard, Filippo Pisano, Marco Pisanello, Antonio Balena, Massimo De Vittorio, Ferruccio Pisanello,
Adaptive optics methods have long been used to perform complex light shaping at the output of a multimode fiber (MMF), with the specific aim of controlling the emitted beam in the near field and enabling the realization of a new generation of endoscopes based on a wide variety of spectroscopic techniques. Gaining control of other emission properties, including the far-field pattern and the phase of the generated beam, would open up the possibility for multimode fibers to act as miniaturized multi- ...
Tópico(s): Optical Coherence Tomography Applications
2021 - AIP Publishing | APL Photonics
Ferruccio Pisanello, Antonio Qualtieri, T. Stomeo, Luigi Martiradonna, R. Cingolani, Alberto Bramati, Massimo De Vittorio,
The optimization of H1 photonic crystal cavities for applications in the visible spectral range is reported, with the goal to obtain a versatile photonic platform to explore strongly and weakly coupled systems. The resonators have been realized in silicon nitride and weakly coupled to both organic (fluorophores) and inorganic (colloidal nanocrystals) nanoparticles emitting in the visible spectral range. The theoretical Purcell factor of the two dipolelike modes in the defect has been increased up ...
Tópico(s): Plasmonic and Surface Plasmon Research
2010 - Optica Publishing Group | Optics Letters
Marco Bianco, Marco Pisanello, Antonio Balena, Cinzia Montinaro, Filippo Pisano, Barbara Spagnolo, Bernardo L. Sabatini, Massimo De Vittorio, Ferruccio Pisanello,
The field of implantable optical neural interfaces has recently enabled the interrogation of neural circuitry with both cell-type specificity and spatial resolution in sub-cortical structures of the mouse brain. This generated the need to integrate multiple optical channels within the same implantable device, motivating the requirement of multiplexing and demultiplexing techniques. In this article, we present an orthogonalization method of the far-field space to introduce mode-division demultiplexing ...
Tópico(s): Advanced Fluorescence Microscopy Techniques
2022 - AIP Publishing | APL Photonics
Filippo Pisano, Marco Pisanello, Leonardo Sileo, Antonio Qualtieri, Bernardo L. Sabatini, Massimo De Vittorio, Ferruccio Pisanello,
With the advent of optogenetic techniques, a major need for precise and versatile light-delivery techniques has arisen from the neuroscience community. Driven by this demand, research on innovative illuminating devices has opened previously inaccessible experimental paths. However, tailoring light delivery to functionally and anatomically diverse brain structures still remains a challenging task. We progressed in this endeavor by micro-structuring metal-coated tapered optical fibers and exploiting ...
Tópico(s): Neuroscience and Neuropharmacology Research
2018 - Elsevier BV | Microelectronic Engineering
Massimo De Vittorio, Ferruccio Pisanello, Luigi Martiradonna, Antonio Qualtieri, T. Stomeo, Alberto Bramati, R. Cingolani,
Abstract Single colloidal quantum dots (QDs) are increasingly exploited as triggered sources of single photons. This review reports on recent results on single photon sources (SPS) based on colloidal quantum dots, whose size, shape and optical properties can be finely tuned by wet chemistry approach. First, we address the optical properties of different colloidal nanocrystals, such as dots, rods and dot in rods and their use as single photon sources will be discussed. Then, we describe different techniques ...
Tópico(s): Photonic Crystals and Applications
2009 - Elsevier BV | Opto-Electronics Review
Luigi Martiradonna, Ferruccio Pisanello, T. Stomeo, Antonio Qualtieri, Giuseppe Vecchio, Stefania Sabella, R. Cingolani, Massimo De Vittorio, Pier Paolo Pompa,
We propose a technological approach aimed at improving biochips performances, based on an efficient spectral modeling and enhancement of markers fluorescence through the insertion of photonic crystal nanocavities (PhC-NCs) in the readout area of biochips. This strategy univocally associates a specific emission wavelength to a specific bioprobe immobilized on a nanocavity, therefore guaranteeing parallel detection of multiple elements and faster analysis time. Moreover, PhC-NCs significantly enhance ...
Tópico(s): Plasmonic and Surface Plasmon Research
2010 - American Institute of Physics | Applied Physics Letters
Marco Pisanello, Di Zheng, Antonio Balena, Filippo Pisano, Massimo De Vittorio, Ferruccio Pisanello,
Two-photon polymerization is a widely adopted technique for direct fabrication of 3D and 2D structures with sub-diffraction-limit features. Here we present an open-hardware, open-software custom design for a holographic multibeam two-photon polymerization system based on a phase-only spatial light modulator and a three-mirror scanhead. The use of three reflective surfaces, two of which scanning the phase-modulated image along the same axis, allows to overcome the loss of virtual conjugation within ...
Tópico(s): Nanofabrication and Lithography Techniques
2022 - Public Library of Science | PLoS ONE
Antonio Di Monaco, Isabella Bruno, Alfonso Sestito, Priscilla Lamendola, Lucy Barone, Anna Bagnato, Roberto Nerla, Chiara Pisanello, Alessandro Giordano, Gaetano Antonio Lanza, Filippo Crea,
To assess whether abnormalities in cardiac uptake of (123)I-metaiodobenzylguanidine (MIBG) correlate with coronary microvascular dysfunction in patients with cardiac syndrome X (CSX).University hospital.29 patients (aged 59 (SD 7) years, 11 men) with typical CSX and a matched group of 20 healthy subjects (aged 56 (7) years, 8 men) were studied.Planar and single photon emission computed tomography (SPECT) MIBG myocardial scintigraphy was performed in all subjects. Coronary flow response (CFR) to adenosine ...
Tópico(s): Cardiovascular Function and Risk Factors
2008 - BMJ | Heart
Emanuela Maglie, Marco Pisanello, Filippo Pisano, Antonio Balena, Marco Bianco, Barbara Spagnolo, Leonardo Sileo, Bernardo L. Sabatini, Massimo De Vittorio, Ferruccio Pisanello,
Tapered optical fibers (TFs) were recently employed for depth-resolved monitoring of functional fluorescence in subcortical brain structures, enabling light collection from groups of a few cells through small optical windows located on the taper edge [Pisano et al ., Nat. Methods 16 , 1185 ( 2019 ) 1548-7091 10.1038/s41592-019-0581-x ]. Here we present a numerical model to estimate light collection properties of microstructured TFs implanted in scattering brain tissue. Ray tracing coupled with the Henyey–Greenstein ...
Tópico(s): Optical Coherence Tomography Applications
2020 - Optica Publishing Group | Optics Letters
Filippo Pisano, Antonio Balena, Muhammad Fayyaz Kashif, Marco Pisanello, Gaia de Marzo, Luciana Algieri, Antonio Qualtieri, Leonardo Sileo, T. Stomeo, A. D’Orazio, Massimo De Vittorio, Ferruccio Pisanello, Marco Grande,
Plasmonic platforms are a promising solution for the next generation of low-cost, integrated biomedical sensors. However, fabricating these nanostructures often requires lengthy and challenging fabrication processes. Here we exploit the peculiar features of Focused Ion Beam milling to obtain single-step patterning of a plasmonic two-dimensional (2D) array of truncated gold nano-pyramids (TNP), with gaps as small as 17 nm. We describe the formation of plasmonic bandgaps in the arrangement of crossed ...
Tópico(s): Optical Coatings and Gratings
2020 - IOP Publishing | Nanotechnology
... Director, The Folger Shakespeare LibraryThe unfinished frescoes by Antonio Pisanello in the Ducal Palace in Mantua have intrigued ...
Tópico(s): Renaissance Literature and Culture
1990 - Truman State University | Sixteenth Century Journal
Leonardo Lamanna, Francesco Rizzi, Christian Demitri, Marco Pisanello, Elisa Scarpa, Antonio Qualtieri, Alessandro Sannino, Massimo De Vittorio,
Tópico(s): Electrostatics and Colloid Interactions
2018 - Springer Science+Business Media | Cellulose
Marco Pisanello, Filippo Pisano, Minsuk Hyun, Emanuela Maglie, Antonio Balena, Massimo De Vittorio, Bernardo L. Sabatini, Ferruccio Pisanello,
Fiber photometry is used to monitor signals from fluorescent indicators in genetically-defined neural populations in behaving animals. Recently, fiber photometry has rapidly expanded and it now provides researchers with increasingly powerful means to record neural dynamics and neuromodulatory action. However, it is not clear how to select the optimal fiber optic given the constraints and goals of a particular experiment. Here, using combined confocal/2-photon microscope, we quantitatively characterize ...
Tópico(s): Circadian rhythm and melatonin
2019 - Frontiers Media | Frontiers in Neuroscience
Antonio Qualtieri, Ferruccio Pisanello, Marco Grande, T. Stomeo, Luigi Martiradonna, Gianmichele Epifani, Angela Fiore, A. Passaseo, Massimo De Vittorio,
In this work we report on our recent studies on Silicon–Nitride PhC single-defect nanocavity membranes embedding colloidal nanocrystals. A novel structure consisting of a layer of nanocrystals sandwiched between two layers of Silicon–Nitride has been used. Photoluminescence measurements prove the efficient coupling among the nanoemitters and the optical modes localized in the Si3N4 photonic crystal nanocavities, showing enhancement of the spontaneous emission in resonant conditions. This technology ...
Tópico(s): Silicon Nanostructures and Photoluminescence
2009 - Elsevier BV | Microelectronic Engineering
Celso Accoto, Antonio Qualtieri, Ferruccio Pisanello, Carlo Ricciardi, Candido Fabrizio Pirri, Massimo De Vittorio, Francesco Rizzi,
We present the optical realization and characterization of complex suspended microchannel resonator (SMR) for biomechanical sensing applications. We exploit the flexibility of two-photon direct laser writing to optimize a highly versatile fabrication strategy based on a shell-writing procedure with the aim to reduce fabrication time of big inlet/outlet sections compatible with most microfluidic systems for lab-on-chip. Compared with standard microfabrication techniques, requiring several technological ...
Tópico(s): Analytical Chemistry and Sensors
2014 - Institute of Electrical and Electronics Engineers | Journal of Microelectromechanical Systems
Massimo Mariello, Francesco Guido, Luciana Algieri, Vincenzo Mastronardi, Antonio Qualtieri, Ferruccio Pisanello, Massimo De Vittorio,
Thin-film piezoelectric materials are currently employed in micro- and nanodevices for energy harvesting and mechanical sensing. The deposition of these functional layers, however, is quite challenging onto non-rigid/non-flat substrates, such as optical fibers (OFs). Besides the recent novel applications of OFs as probes for biosensing and bioactuation, the possibility to combine them with piezoelectric thin films and metallic electrodes can pave the way for the employment of novel opto-electro-mechanical ...
Tópico(s): Advanced Sensor and Energy Harvesting Materials
2020 - Institute of Electrical and Electronics Engineers | IEEE Transactions on Nanotechnology
Marco Bianco, Antonio Balena, Marco Pisanello, Filippo Pisano, Leonardo Sileo, Barbara Spagnolo, Cinzia Montinaro, Bernardo L. Sabatini, Massimo De Vittorio, Ferruccio Pisanello,
As the scientific community seeks efficient optical neural interfaces with sub-cortical structures of the mouse brain, a wide set of technologies and methods is being developed to monitor cellular events through fluorescence signals generated by genetically encoded molecules. Among these technologies, tapered optical fibers (TFs) take advantage of the modal properties of narrowing waveguides to enable both depth-resolved and wide-volume light collection from scattering tissue, with minimized invasiveness ...
Tópico(s): Neuroscience and Neural Engineering
2020 - Optica Publishing Group | Biomedical Optics Express
Filippo Pisano, Muhammad Fayyaz Kashif, Antonio Balena, Marco Pisanello, Francesco De Angelis, Liset Menéndez de la Prida, Manuel Valiente, A. D’Orazio, Massimo De Vittorio, Marco Grande, Ferruccio Pisanello,
Abstract Optical methods are driving a revolution in neuroscience. Ignited by optogenetic techniques, a set of strategies has emerged to control and monitor neural activity in deep brain regions using implantable photonic probes. A yet unexplored technological leap is exploiting nanoscale light‐matter interactions for enhanced bio‐sensing, beam‐manipulation and opto‐thermal heat delivery in the brain. To bridge this gap, we got inspired by the brain cells’ scale to propose a nano‐patterned tapered‐ ...
Tópico(s): Neuroscience and Neural Engineering
2021 - Wiley | Advanced Optical Materials
Di Zheng, Filippo Pisano, Liam Collard, Antonio Balena, Marco Pisanello, Barbara Spagnolo, Rosa Mach‐Batlle, Francesco Tantussi, Luigi Carbone, Francesco De Angelis, Manuel Valiente, Liset Menéndez de la Prida, Cristian Ciracì, Massimo De Vittorio, Ferruccio Pisanello,
Integration of plasmonic nanostructures with fiber-optics-based neural probes enables label-free detection of molecular fingerprints via surface-enhanced Raman spectroscopy (SERS), and it represents a fascinating technological horizon to investigate brain function. However, developing neuroplasmonic probes that can interface with deep brain regions with minimal invasiveness while providing the sensitivity to detect biomolecular signatures in a physiological environment is challenging, in particular ...
Tópico(s): Molecular Communication and Nanonetworks
2022 - | Advanced Materials
Barbara Spagnolo, Antonio Balena, Rui T. Peixoto, Marco Pisanello, Leonardo Sileo, Marco Bianco, Alessandro Rizzo, Filippo Pisano, Antonio Qualtieri, Dario Domenico Lofrumento, Francesco De Nuccio, John A. Assad, Bernardo L. Sabatini, Massimo De Vittorio, Ferruccio Pisanello,
Deciphering the neural patterns underlying brain functions is essential to understanding how neurons are organized into networks. This deciphering has been greatly facilitated by optogenetics and its combination with optoelectronic devices to control neural activity with millisecond temporal resolution and cell type specificity. However, targeting small brain volumes causes photoelectric artefacts, in particular when light emission and recording sites are close to each other. We take advantage of ...
Tópico(s): Neural dynamics and brain function
2022 - Nature Portfolio | Nature Materials
Filippo Pisano, Marco Pisanello, Suk Joon Lee, Jaeeon Lee, Emanuela Maglie, Antonio Balena, Leonardo Sileo, Barbara Spagnolo, Marco Bianco, Minsuk Hyun, Massimo De Vittorio, Bernardo L. Sabatini, Ferruccio Pisanello,
Fiber photometry is increasingly utilized to monitor fluorescent sensors of neural activity in the brain. However, most implementations are based on flat-cleaved optical fibers that can only interface with shallow tissue volumes adjacent to the fiber. We exploit modal properties of tapered optical fibers (TFs) to enable light collection over an extent of up to 2 mm of tissue and multisite photometry along the taper. Using a single TF, we simultaneously observed distinct dopamine transients in dorsal ...
Tópico(s): Neural dynamics and brain function
2019 - Nature Portfolio | Nature Methods
Ferruccio Pisanello, Luigi Martiradonna, Antonio Qualtieri, T. Stomeo, Marco Grande, Pier Paolo Pompa, R. Cingolani, Alberto Bramati, Massimo De Vittorio,
We propose silicon nitride two-dimensional photonic crystal resonators as flexible platform to realize photonic devices based on spontaneous emission engineering of nanoemitters in the visible spectral range.The versatility of our approach is demonstrated by coupling the two dipole-like modes of a closed band gap H1 nanocavity with: (i) DNA strands marked with Cyanine 3 organic dyes, (ii) antibodies bounded to fluorescent proteins and (iii) colloidal semiconductor nanocrystals localized in the maximum ...
Tópico(s): Plasmonic and Surface Plasmon Research
2011 - Elsevier BV | Photonics and Nanostructures - Fundamentals and Applications
Cinzia Montinaro, Marco Pisanello, Marco Bianco, Barbara Spagnolo, Filippo Pisano, Antonio Balena, Francesco De Nuccio, Dario Domenico Lofrumento, Tiziano Verri, Massimo De Vittorio, Ferruccio Pisanello,
Fiber photometry is widely used in neuroscience labs for in vivo detection of functional fluorescence from optical indicators of neuronal activity with a simple optical fiber. The fiber is commonly placed next to the region of interest to both excite and collect the fluorescence signal. However, the path of both excitation and fluorescence photons is altered by the uneven optical properties of the brain, due to local variation of the refractive index, different cellular types, densities and shapes. ...
Tópico(s): Circadian rhythm and melatonin
2021 - Optica Publishing Group | Biomedical Optics Express
Di Zheng, Filippo Pisano, Liam Collard, Antonio Balena, Marco Pisanello, Barbara Spagnolo, Rosa Mach‐Batlle, Francesco Tantussi, Luigi Carbone, Francesco De Angelis, Manuel Valiente, Liset Menéndez de la Prida, Cristian Ciracì, Massimo De Vittorio, Ferruccio Pisanello,
... number 2200902, Di Zheng, Massimo De Vittorio, Ferruccio Pisanello, and co-workers present a surface-enhanced Raman scattering (SERS)-active probe based on a tapered optical fiber decorated with sub-10 nm-gap gold nanoislands, which is designed for neurochemical detection. Through-fiber measurements show the fabricated device can detect neurotransmitters down to the micromolar range. Cover image artwork by: Antonio Balena, Barbara Spagnolo, and Di Zheng.
Tópico(s): Spectroscopy Techniques in Biomedical and Chemical Research
2023 - | Advanced Materials
Tópico(s): Yersinia bacterium, plague, ectoparasites research
1992 - | Pferdeheilkunde Equine Medicine
Antonio Piccoli, Luana Pillon, Lorena Pisanello, Graziella Zacchello,
1999 - Elsevier BV | Nutrition
Antonio Balena, Marco Bianco, Ferruccio Pisanello, Massimo De Vittorio,
Abstract Two‐Photon Lithography, thanks to its very high sub‐diffraction resolution, has become the lithographic technique par excellence in applications requiring small feature sizes and complex 3D pattering. Despite this, the fabrication times required for extended structures remain much longer than those of other competing techniques (UV mask lithography, nanoimprinting, etc.). Its low throughput prevents its wide adoption in industrial applications. To increase it, over the years different solutions ...
Tópico(s): Nanofabrication and Lithography Techniques
2023 - Wiley | Advanced Functional Materials
Enrico Domenico Lemma, Francesco Rizzi, Tommaso Dattoma, Barbara Spagnolo, Leonardo Sileo, Antonio Qualtieri, Massimo De Vittorio, Ferruccio Pisanello,
Two-photon (2P) lithography shows great potential for the fabrication of three-dimensional (3-D) micro- and nanomechanical elements, for applications ranging from microelectromechanical systems to tissue engineering, by virtue of its high resolution (<;100 nm) and biocompatibility of the photosensitive resists. However, there is a considerable lack of quantitative data on mechanical properties of materials for 2P lithography and of structures obtained through this technique. In this paper, we combined ...
Tópico(s): Cellular Mechanics and Interactions
2016 - Institute of Electrical and Electronics Engineers | IEEE Transactions on Nanotechnology
Liam Collard, Filippo Pisano, Di Zheng, Antonio Balena, Muhammad Fayyaz Kashif, Marco Pisanello, Antonella D’Orazio, Liset Menéndez de la Prida, Cristian Ciracì, Marco Grande, Massimo De Vittorio, Ferruccio Pisanello,
Integration of plasmonic structures on step-index optical fibers is attracting interest for both applications and fundamental studies. However, the possibility to dynamically control the coupling between the guided light fields and the plasmonic resonances is hindered by the turbidity of light propagation in multimode fibers (MMFs). This pivotal point strongly limits the range of studies that can benefit from nanostructured fiber optics. Fortunately, harnessing the interaction between plasmonic modes ...
Tópico(s): Orbital Angular Momentum in Optics
2022 - Wiley | Small
... evidence its influence. My specific illustration comes from Antonio Pisano, known as Pisanello, who worked in the first half of the ...
Tópico(s): Legal Systems and Judicial Processes
1997 - Washington and Lee University School of Law | Washington and Lee law review