Produção Nacional
Revisado por pares
Bruna Lannuce Silva Cabral, Artur Christian Garcia da Silva, Renato Ivan de Ávila, Alane Pereira Cortez, Rangel Magalhães Luzin, Luciano M. Lião, Eric de Souza Gil, Germán Sanz Lobón, Boniek G. Vaz, J.R. Sabino, Ricardo Menegatti, Marize Campos Valadares,
This study shows the design, synthesis and antitumoral potential evaluation of a novel chalcone-like compound, (E)-3- (3, 5-di-ter-butyl-4-hydroxyphenyl)-1- (4-hydroxy-3-methoxyphenyl) prop-2-en-1-one [LQFM064) (4)], against human breast adenocarcinoma MCF7 cells. Some toxicological parameters were also investigated. LQFM064) (4) exhibited cytotoxic activity against MCF7 cells (IC50 = 21 μM), in a concentration dependent-manner, and triggered significant changes in cell morphology and biochemical/molecular ...
Tópico(s): Bioactive Compounds and Antitumor Agents
2017 - Elsevier BV | European Journal of Pharmaceutical Sciences
Produção Nacional
Revisado por pares
Relton Romeis Oliveira, Emílio R. Cintra, Ailton A. Sousa-Junior, Larissa C. Moreira, Artur Christian Garcia da Silva, Ana Luiza Ribeiro de Souza, Marize Campos Valadares, Marcus S. Carrião, Andris F. Bakuzis, Eliana Martins Lima,
Melanoma is the most aggressive and metastasis-prone form of skin cancer. Conventional therapies include chemotherapeutic agents, either as small molecules or carried by FDA-approved nanostructures. However, systemic toxicity and side effects still remain as major drawbacks. With the advancement of nanomedicine, new delivery strategies emerge at a regular pace, aiming to overcome these challenges. Stimulus-responsive drug delivery systems might considerably reduce systemic toxicity and side-effects ...
Tópico(s): Erythrocyte Function and Pathophysiology
2023 - Multidisciplinary Digital Publishing Institute | Pharmaceutics
Produção Nacional
Revisado por pares
Emílio R. Cintra, Tácio Gonçalves Hayasaki, Ailton A. Sousa-Junior, Artur Christian Garcia da Silva, Marize Campos Valadares, Andris F. Bakuzis, Sebastião Antônio Mendanha, Eliana Martins Lima,
Doxorubicin (DOX) is a chemotherapeutic agent commonly used for the treatment of solid tumors. However, the cardiotoxicity associated with its prolonged use prevents further adherence and therapeutic efficacy. By encapsulating DOX within a PEGylated liposome, Doxil® considerably decreased DOX cardiotoxicity. By using thermally sensitive lysolipids in its bilayer composition, ThermoDox® implemented a heat-induced controlled release of DOX. However, both ThermoDox® and Doxil® rely on their passive ...
Tópico(s): Graphene and Nanomaterials Applications
2022 - Frontiers Media | Frontiers in Pharmacology
Produção Nacional
Revisado por pares
Soraia F. Bezerra, Bruna dos Santos Rodrigues, Artur Christian Garcia da Silva, Renato Ivan de Ávila, H. Brito, Emílio R. Cintra, Danillo Fabrini Maciel Costa Veloso, Eliana Martins Lima, Marize Campos Valadares,
Abstract Background Currently, considerable efforts to standardize methods for accurate assessment of properties and safety aspects of nanomaterials are being made. However, immunomodulation effects upon skin exposure to nanomaterial have not been explored. Objectives To investigate the immunotoxicity of single‐wall carbon nanotubes, titanium dioxide, and fullerene using the current mechanistic understanding of skin sensitization by applying the concept of adverse outcome pathway (AOP). Methods Investigation ...
Tópico(s): Immunotoxicology and immune responses
2020 - Wiley | Contact Dermatitis
Produção Nacional
Revisado por pares
Bruna Ferreira Tollstadius, Artur Christian Garcia da Silva, Bruna Cristiane Oliveira Pedralli, Marize Campos Valadares,
The fungicide Carbendazim is widely used in agriculture and preservation of films and fibers. In mammals, it can promote germ cell mutagenicity, carcinogenicity, and reproductive toxicity. However, few data about the effects of this toxicant upon the respiratory system are available. In this work, we evaluated Carbendazim toxicity upon A549 alveolar cells both in monolayer and upon air-liquid interface cell system. Monolayer cell exposed to non-cytotoxic concentrations of this fungicide showed cell ...
Tópico(s): Pesticide Exposure and Toxicity
2019 - Elsevier BV | Toxicology in Vitro
Produção Nacional
Revisado por pares
Edilson Ribeiro de Oliveira, Thaís Leite Nascimento, Mariana Arraes Salomão, Artur Christian Garcia da Silva, Marize Campos Valadares, Eliana Martins Lima,
Tópico(s): Neonatal Respiratory Health Research
2019 - Springer Science+Business Media | Pharmaceutical Research
Produção Nacional
Revisado por pares
Edvande Xavier dos Santos Filho, Artur Christian Garcia da Silva, Renato Ivan de Ávila, Aline Carvalho Batista, Ricardo Neves Marreto, Eliana Martins Lima, Cecília Maria Alves de Oliveira, Elismauro Francisco Mendonça, Marize Campos Valadares,
This study evaluated the mechanisms involved in the chemopreventive effects of a mucoadhesive formulation (FITOPROT), containing curcuminoids from Curcuma longa L. (Zingiberaceae) and Bidens pilosa L. (Asteraceae) extract, against 5-FU-induced cellular toxicity using an in vitro oral mucositis model.Effects of FITOPROT on 5-FU-induced cytotoxicity in HaCaT and SSC-4 cells were evaluated by MTT assay. For mechanistic analyses, HaCaT cells were first pretreated with FITOPROT (0.005%) for 24h followed ...
Tópico(s): Oral Health Pathology and Treatment
2017 - Elsevier BV | Life Sciences
Produção Nacional
Revisado por pares
Maiulle Teixeira Pacheco, Artur Christian Garcia da Silva, Thaís Leite Nascimento, Danielle Guimarães Almeida Diniz, Marize Campos Valadares, Eliana Martins Lima,
Abstract Objectives Bioactive molecules derived from natural products combine the ability to absorb UV light and act as antioxidants. We developed an oil-based sucupira (native species of the Brazilian cerrado) nanoemulsion (NE) using a high-energy emulsification method and assessed its effectiveness in vitro. Methods An easily scalable high-pressure homogenization method was used to prepare the formulation. NE droplets mean diameter, pH, stability, conductivity and morphology were analysed. Formulation ...
Tópico(s): Essential Oils and Antimicrobial Activity
2019 - Oxford University Press | Journal of Pharmacy and Pharmacology
Produção Nacional
Revisado por pares
M. Rigoni, João Carlos Garcia, Artur Posenato Garcia, Pedro Da Silva, Nelson Jhoe Batistela, Patrick Kuo‐Peng,
This study has analyzed the performance of an alternating electric current measuring system using a Rogowski coil as a transducer, applied to magnitudes of milliamps to hundreds of amperes. It has been noticed that for low amplitudes, as well as for low frequencies, noise interferes with precision. For operation levels with a better signal-to-noise rate, the results presented agreements with those from a Hall effect current probe. Although prototypes have been carefully constructed, performance ...
Tópico(s): Geophysical and Geoelectrical Methods
2008 - Institute of Electrical and Electronics Engineers | IEEE Potentials
Produção Nacional
Revisado por pares
Lorrane Kelle da Silva Moreira, Rafaela Ribeiro Silva, Dayane Moreira da Silva, Mirella Andrade Silva Mendes, Adriane Ferreira de Brito, Flávio Souza de Carvalho, Germán Sanz Lobón, Marcella Ferreira Rodrigues, Artur Christian Garcia da Silva, Douglas Vieira Thomaz, Valéria de Oliveira, Boniek G. Vaz, Luciano M. Lião, Marize Campos Valadares, Eric de Souza Gil, Élson Alves Costa, François Noël, Ricardo Menegatti,
The current treatments available for anxiety and depression are only palliative. Full remission has remained elusive, characterizing unmet medical needs. In the scope of an academic drug discovery program, we describe here the design, synthesis, in vitro metabolism prediction and pharmacological characterization of a new piperazine compound, 1-(4-methoxyphenyl)−4-((1-phenyl-1H-pyrazol-4-yl)methyl)piperazine (LQFM005), and of its main putative metabolite, 4-(4-((4-(4-methoxyphenyl)piperazin-1-yl)methyl)− ...
Tópico(s): Chemical synthesis and alkaloids
2021 - Elsevier BV | Behavioural Brain Research
Produção Nacional
Revisado por pares
Diego Antônio Costa Arantes, Artur Christian Garcia da Silva, Nilceana Maya Aires Freitas, Eliana Martins Lima, Aline Carlos de Oliveira, Ricardo Neves Marreto, Elismauro Francisco Mendonça, Marize Campos Valadares,
Abstract Background Oral mucositis (OM) is the significant complication of radio/chemotherapy treatment. This study evaluated the safety and efficacy of a mucoadhesive phytomedication containing curcuminoids and Bidens pilosa L . (FITOPROT) in the prevention/treatment of OM. Methods Sixty‐two patients were randomized into the group's intervention and placebo. Adverse effect assessment, OM grading, pain, and saliva collection were carried at the 1st, 15th, 21st, and final of radiotherapy (RT). Inflammatory ...
Tópico(s): Oral Health Pathology and Treatment
2021 - Wiley | Head & Neck
Produção Nacional
Revisado por pares
Artur Christian Garcia da Silva, Isabelly Paula Sousa, Thaís Rosa Marques dos Santos, Marize Campos Valadares,
Abstract Agriculture in the 21st century faces multiple challenges to produce food for the growing population using ethical/sustainable and efficient methods safely for humans and the environment. Brazil today is a world leader in terms of production of food of plant origin, both for human consumption and animal feed. Agriculture and livestock raising are critical economic activities in maintaining a positive balance in its economy. As a consequence, the registration and use of pesticides in Brazil ...
Tópico(s): Pesticide Residue Analysis and Safety
2020 - Oxford University Press | Toxicological Sciences
Produção Nacional
Revisado por pares
Artur Christian Garcia da Silva, Adrienny Rodrigues Chialchia, Renato Ivan de Ávila, Marize Campos Valadares,
Eye toxicity is a mandatory parameter in human risk and safety evaluation for products including chemicals, pesticides, medicines and cosmetics. Historically, this endpoint has been evaluated using the Draize rabbit eye test, an in vivo model that was never formally validated. Due to advances in scientific knowledge, economic and ethical issues, non-animal methods based on mechanisms of toxicity are being developed and validated for increasing the capability of these models to predict eye toxicity. ...
Tópico(s): Immune Response and Inflammation
2018 - Elsevier BV | Chemico-Biological Interactions
Produção Nacional
Revisado por pares
Gustavo Mota Galvão, Iziara Ferreira Florentino, Germán Sanz Lobón, Boniek G. Vaz, Luciano M. Lião, J.R. Sabino, Carina Sofia Cardoso, Daiany Priscilla Bueno da Silva, Élson Alves Costa, Andreia L.P. Silva, Artur Christian Garcia da Silva, Marize Campos Valadares, Jacqueline Alves Leite, Eric de Souza Gil, Ricardo Menegatti,
LQFM219 is a molecule designed from celecoxibe (COX-2 inhibitor) and darbufelone (inhibitor of COX-2 and 5-LOX) lead compounds through a molecular hybridisation strategy. Therefore, this work aimed to investigate the antinociceptive and anti-inflammatory activities of this new hybrid compound. The acute oral systemic toxicity of LQFM219 was evaluated via the neutral red uptake assay. Acetic acid-induced abdominal writhing and CFA-induced mechanical hyperalgesia were performed to evaluate the antinociceptive ...
Tópico(s): Pain Mechanisms and Treatments
2020 - Elsevier BV | International Immunopharmacology
Produção Nacional
Revisado por pares
Leandro de Jesus Jorge, Verônica Scarpini Cândido, Alisson Clay Rios da Silva, Fábio da Costa Garcia Filho, Artur Camposo Pereira, Fernanda Santos da Luz, Sérgio Neves Monteiro,
This paper aims to present a comparison on the use of different heat treatment conditions in association with welding of a HSLA-80 steel. This investigation was carried out by multipass welding using the coated electrode process in different heat treatment conditions, with or without pre- or post-weld heating, in order to compare effects on the mechanical properties and microstructure for each condition. After welding was performed, procedures to identify microstructural phases and characterize ...
Tópico(s): Hydrogen embrittlement and corrosion behaviors in metals
2018 - Elsevier BV | Journal of Materials Research and Technology
Produção Nacional
Revisado por pares
Higor de Oliveira Ribeiro, Alane Pereira Cortez, Renato Ivan de Ávila, Artur Christian Garcia da Silva, Flávio Silva de Carvalho, Ricardo Menegatti, Luciano M. Lião, Marize Campos Valadares,
Abstract Our group designed and synthesized the N‐phenyl‐piperazine LQFM030 [1‐(4‐((1‐(4‐chlorophenyl)‐1H–pyrazol‐4‐yl)methyl) piperazin‐1‐yl) ethanone], a small molecule derived from molecular simplification of the Nutlin‐1, an inhibitor of the human homologue of murine double minute 2 (MDM2) protein that is expressed in several types of cancer. To better investigate the effects of LQFM030 regarding the p53 mutation status, this study investigated the antiproliferative activity of LQFM030 against ...
Tópico(s): Cell death mechanisms and regulation
2020 - Wiley | Fundamental and Clinical Pharmacology
Produção Nacional
Revisado por pares
Artur Christian Garcia da Silva, Adrienny Rodrigues Chialchia, Elisandra Gava de Castro, Marlos R.L. e Silva, Diego Antônio Costa Arantes, Aline Carvalho Batista, Gregory T. Kitten, Marize Campos Valadares,
In vitro eye toxicity assessment using reconstructed corneal epithelial models has emerged highlighting its applicability domain for Classification and Labeling of products and chemicals. However, due to bureaucratic issues, such models are not commercially available in Brazil and Latin America. In this work, we developed, characterized and evaluated the applicability of a new corneal epithelial biomimetic model using a cell lineage for in vitro eye toxicity assessment. The reconstructed tissue ...
Tópico(s): 3D Printing in Biomedical Research
2019 - Elsevier BV | Toxicology in Vitro
Produção Nacional
Revisado por pares
Artur Christian Garcia da Silva, Sérgio de Morais Carvalho Filho, Marize Campos Valadares,
Tópico(s): Allergic Rhinitis and Sensitization
2023 - Elsevier BV | Toxicology in Vitro
... following articles citing this article:Thiago Costa Chacon, Artur Garcia Gonçalves, Lucas Ferreira da Silva , Forma y Función 32, no.22 ( 2019): 41. ...
Tópico(s): Categorization, perception, and language
2009 - University of Chicago Press | International Journal of American Linguistics
Produção Nacional
Revisado por pares
Artur M. S. Silva, Márcio Garcia Ribeiro, Félix Carvalho, Valéria Moreira da Silva, Léa Assed Bezerra da Silva,
The effects of continuous sugarcane (Saccharum officinarum) cropping on the properties of a cohesive Yellow Latosol were studied in the region of the Coastal Tablelands, Northeast Brazil. Four areas were studied at Caeté mill, municipality of São Miguel dos Campos, Alagoas State, involving a native forest (Tn), and sugarcane fields cultivated for periods of 2 years (T2), 18 years (T18) and 25 years (T25). Samples were collected from each area at 0–0.2 and 0.2–0.4 m depth, to determine total organic C, ...
Tópico(s): Sugarcane Cultivation and Processing
2006 - Elsevier BV | Soil and Tillage Research
Catarina Prado e Castro, Artur R. M. Serrano, Pedro Martins da Silva, María Dolores García García,
Information on Diptera community, seasonality and successional patterns in every geographical region is fundamental for the use of flies as forensic indicators of time of death. In order to obtain these data from the Lisbon area (Portugal), experiments were conducted during the four seasons of the year, using piglet carcasses as animal models. Five stages were recognized during the decomposition process. The stages, besides visually defined, could be separated taking into account the occurrence and ...
Tópico(s): Diptera species taxonomy and behavior
2012 - Wiley | Medical and Veterinary Entomology
Produção Nacional
Marize Campos Valadares, Gisele Augusto Rodrigues de Oliveira, Renato Ivan de Ávila, Artur Christian Garcia da Silva,
Historically, the ocular toxicity of manufactured consumer materials has been evaluated using the rabbit in vivo Draize rabbit eye test. The animal data obtained were used by the United Nations Globally Harmonized System of Classification and Labelling of Chemicals (UN GHS) to define the classification and labelling (C&L) for eye damage/irritation endpoint. However, the Draize test, a method which was never formally validated, has been widely criticized because of its technical limitations. In addition, ...
Tópico(s): Pesticide Exposure and Toxicity
2021 - Springer Science+Business Media | Methods in molecular biology
Produção Nacional
Revisado por pares
Wanessa Machado Andrade, Artur Christian Garcia da Silva, Larissa Cleres Moreira, Thaisângela L. Rodrigues, Aline Carvalho Batista, Marize Campos Valadares,
Considering the successful employment of alternative methods for eye toxicity assessment of products for regulatory purposes, and the recent advances in Brazilian legislative scenario, which adopted the UN GHS classification system for agrochemical formulations toxicity assessment, there is an emerging demand for strategies that allow the evaluation of such products. Based on this, the present study aimed to address the applicability of a mechanistic-based defined approach for eye toxicity assessment ...
Tópico(s): Environmental Toxicology and Ecotoxicology
2020 - Elsevier BV | Toxicology in Vitro
Produção Nacional
Revisado por pares
Camilla Luiza-Batista, Sabine Thiberge, Malika Serra-Hassoun, Flore Nardella, Aurélie Claës, Vanessa C. Nicolete, Pierre‐Henri Commère, Liliana Mâncio-Silva, Marcelo U. Ferreira, Artur Scherf, Sylvie Garcia,
Abstract Plasmodium vivax is the most widespread human malaria parasite. Due to the presence of extravascular reservoirs and relapsing infections from dormant liver stages, P. vivax is particularly difficult to control and eliminate. Experimental research is hampered by the inability to maintain P. vivax cultures in vitro, due to its tropism for immature red blood cells (RBCs). Here, we describe a new humanized mice model that can support efficient human erythropoiesis and maintain long-lasting multiplication ...
Tópico(s): Mosquito-borne diseases and control
2022 - Nature Portfolio | Nature Communications
Catarina Prado e Castro, María Dolores García García, Pedro Martins da Silva, Israel Faria e Silva, Artur R. M. Serrano,
Some Coleoptera are recognised as being forensically important as post-mortem interval (PMI) indicators, especially in the later stages of cadaver decomposition. Because insect species and their timings of appearance in cadavers vary according to geographic location, it is important to know their succession patterns, as well as seasonality at a regional level. In this study, we aimed to contribute to broaden this knowledge by surveying beetle communities from the Lisbon area during the four seasons ...
Tópico(s): Yersinia bacterium, plague, ectoparasites research
2013 - Elsevier BV | Forensic Science International
Produção Nacional
Revisado por pares
Michelle Souza Oliveira, Fernanda Santos da Luz, Andressa Teixeira Souza, Luana Cristyne da Cruz Demosthenes, Artur Pereira, Fábio da Costa Garcia Filho, Fábio de Oliveira Braga, André Ben‐Hur da Silva Figueiredo, Sérgio Neves Monteiro,
The replacement of synthetic fibers by natural fibers has, in recent decades, been the subject of intense research, particularly as reinforcement of composites. In this work, the lesser known tucum fiber, extracted from the leaves of the Amazon Astrocaryum vulgare palm tree, is investigated as a possible novel reinforcement of epoxy composites. The tucum fiber was characterized by pullout test for interfacial adhesion with epoxy matrix. The fiber presented a critical length of 6.30 mm, with interfacial ...
Tópico(s): Textile materials and evaluations
2020 - Multidisciplinary Digital Publishing Institute | Polymers
Produção Nacional
Revisado por pares
Tatyane Gonçalves Hayasaki, Thaís Rosa Marques dos Santos, Artur Christian Garcia da Silva, Marize Campos Valadares,
Acute inhalation toxicity testing for chemical classification and labeling has been performed using animal models, however, these models have limited predictibility of the toxicity on the respiratory system. Thus, non-animal models have been emerging as alternatives for preclinical assessment of respiratory toxicity of chemicals, comprising in chemico, in vitro, ex vivo, and in silico approaches. In this study, we characterized and evaluated the applicability of a new ex vivo bovine bronchial model ...
Tópico(s): Effects and risks of endocrine disrupting chemicals
2022 - Elsevier BV | Food and Chemical Toxicology
Produção Nacional
Revisado por pares
Diego Antônio Costa Arantes, Artur Christian Garcia da Silva, Eliana Martins Lima, Ellen Cristine Pineze Alonso, Ricardo Neves Marreto, Elismauro Francisco Mendonça, Marize Campos Valadares, Aline Carvalho Batista,
FITOPROT, which contains curcuminoids and Bidens pilosa L. extract, is an innovative mucoadhesive formulation indicated for the topical treatment of chemoradiotherapy-induced oral mucositis (OM) in patients with advanced and visible oral squamous cell carcinoma. The formulation is used as a mouthwash directly on tumor tissue of patients with advanced neoplasms, without triggering cancer cell proliferation or tumor invasiveness. Thus, the aim of this study was to evaluate the biological effects of ...
Tópico(s): Curcumin's Biomedical Applications
2021 - Brazilian Society of Dental Research | Brazilian Oral Research
Produção Nacional
Revisado por pares
Larissa Vivan Cestonaro, Artur Christian Garcia da Silva, Solange Cristina García, Marize Campos Valadares, Marcelo Dutra Arbo,
Abstract Background Pesticides are indispensable for the cultivation of crops, especially those of economic importance, such as soybeans. Data on the annual use of herbicides in crops show that they correspond to 50%, making it the most used in agriculture. Aim Therefore, the aim of this study was to evaluate the toxicity of the three commercial herbicides (clomazone, glyphosate, and sulfentrazone) in THP-1 cells. Methods Cells were incubated with 0–5,000 mg/L of the herbicides for 24 h at 37 °C for ...
Tópico(s): Carcinogens and Genotoxicity Assessment
2024 - Oxford University Press | Toxicology Research
Ana Carla C. Mello e Silva, Artur Serra Neto, Cloves Eduardo S. Galvão, Sirley Garcia Marques, Ana Cristina R. Saldanha, Conceição de Maria Pedroso e Silva, Olga Fischman, Raimunda Ribeiro da Silva, Maria do Rosário da S. R. Costa, Jackson Maurício Lopes Costa,
The aim of this study was to observe the clinical and of epidemiological behavior of chromoblastomycosis in the State of Maranhão. A retrospective and prospective study of 13 cases was performed at the infectious diseases section of the Hospital dos Servidores do Estado do Maranhão, from November, 1988 to July, 1991. In the investigation a protocol record was used with a view to further analysis. A higher prevalence between 50 and 60 years of age (46.1%) and in male patients (92.3%) was noted. Twelve ...
Tópico(s): Antifungal resistance and susceptibility
1992 - Brazilian Society of Tropical Medicine | Revista da Sociedade Brasileira de Medicina Tropical