Claudine Campbell, Thomas Hughes, Fernando Mezzetti, David Guberman, David Kramer, Walter Bloch, Art Durity, Lee Nwandiko,
... Gorbachev's economists Siemens The law is an asno Survivor Schalck Germany Calibra Vauxhall Postcard from a ...
1991 - Gale Group | The Economist
... stephensi (As) have also shown transcriptional activation of AsNOS in the midgut and systemically in response to ... expression of several proteins, such as AsSTAT, vATPase, AsNOS, actin and a P.berghei subtilisin-like serine ... red), NOS (green) and vATPase (blue). (D) Homogeneous AsNOS cytoplasmic staining of epithelial cells at the posterior ... In an initial study, double immunofluorescence staining for AsNOS and Pbs21 was performed. AsNOS was detected using a commercial ‘Universal anti-NOS’ ... from unfed females and pupae (data not shown). AsNOS is constitutively expressed in the cytoplasm of most ...
Tópico(s): Mosquito-borne diseases and control
2000 - Springer Nature | The EMBO Journal
Brice Sagot, Marc Gaysinski, Mohamed Mehiri, Jean‐Marie Guigonis, Daniel Le Rudulier, Geneviève Alloing,
... locus carrying the orthologous genes in S. meliloti, asnO and ngg, was identified, and the genetic and ... experiments, it was found that strains inactivated in asnO and ngg were unable to produce the dipeptide. ... activity from transcriptional fusion revealed strong induction of asnO expression in cells grown in increased NaCl concentration, in good agreement with the NAGGN accumulation. The asnO-ngg cluster encodes a unique enzymatic machinery mediating ... formation of the intermediate N-acetylglutaminylglutamine, and second AsnO, required for subsequent addition of an amide group ...
Tópico(s): Biochemical and Molecular Research
2010 - National Academy of Sciences | Proceedings of the National Academy of Sciences
Hélène Bergès, Claire Checroun, Sébastien Guiral, Anne-Marie Garnerone, P. Boistard, Jacques Batut,
... of the mutant. This gene, that was named asnO, encodes a protein homologous to glutamine-dependent asparagine synthetases. The asnO gene did not appear to affect asparagine biosynthesis ... function in S. meliloti. We provide evidence that asnO is active during symbiosis.Isolation of the asnO mutant argues for the existence of a physiological ... function in S. meliloti. Our data suggest that asnO might control activity of the FixT protein, in ... remains to be elucidated. A proposed role for asnO might be to couple nitrogen fixation gene expression ...
Tópico(s): Wastewater Treatment and Nitrogen Removal
2001 - BioMed Central | BMC Microbiology
Christopher P. Crum, David R. Genest, Jeffrey F. Krane, Christine Hogan, Deqin Sun, Bronya Bellerose, Evanthia Kostopoulou, Kenneth R. Lee,
... ASCUS into "favor reactive"(ASFR), "not otherwise specified" (ASNOS), and "favor squamous intraepithelial lesion (SIL)" (ASFS). This ... Boxborough, MA) specimens previously diagnosed as normal, ASFR, ASNOS, ASFS, and SIL Interobserver reproducibility was computed with ... good (κ = .68 and .63). Reproducibility for ASFR, ASNOS, and ASFS ranged from poor to fair (κ = . ... 32). In a weighted analysis, lvalues for ASFR/ASNOS and ASFS/SIL were .36 and .62, respectively. ... positivity for preparations originally diagnosed as N ASFR, ASNOS, ASFS, and SIL were 5.7%, 8.8%, ...
Tópico(s): Genital Health and Disease
1999 - Oxford University Press | American Journal of Clinical Pathology
Ken‐ichi Yoshida, Yasutaro Fujita, S. Dusko Ehrlich,
ABSTRACT Three asparagine synthetase genes, asnB , asnH , and asnO ( yisO ), were predicted from the sequence of the ... exponential growth and stationary phase, and that of asnO was seen only later in sporulation. In a ... and in stationary phase, while the expression of asnO was not detected in either phase. However, when ... comprising yxbB , yxbA , yxnB , asnH , and yxaM . The asnO gene is a monocistronic operon, the expression of ... an asparagine synthetase. In B. subtilis , deletion of asnO or asnH , singly or in combination, had essentially ...
Tópico(s): Amino Acid Enzymes and Metabolism
1999 - American Society for Microbiology | Journal of Bacteriology
Matthias Strieker, Florian Kopp, Christoph Mahlert, Lars‐Oliver Essen, Mohamed A. Marahiel,
... antibiotic (CDA). Direct hydroxylation of l-asparagine by AsnO, a non-heme Fe2+/α-ketoglutarate-dependent oxygenase ... 92, and 1.66 Å crystal structures of AsnO as apoprotein, Fe2+ complex, and product complex, respectively, ... succinate revealed the stereoselectivity and substrate specificity of AsnO. The comparison of native and product-complex structures of AsnO showed a lid-like region (residues F208–E223) ... its incorporation into the growing CDA peptide. The AsnO structure could serve as a template for engineering ...
Tópico(s): Microbial Natural Products and Biosynthesis
2007 - American Chemical Society | ACS Chemical Biology
Ian Price, Bard Ermentrout, Rubén Zamora, Bo Wang, Nabil Azhar, Qi Mi, Gregory Constantine, James R. Faeder, Shirley Luckhart, Yoram Vodovotz,
... mosquito, ingested TGF-β1 induces A. stephensi NOS (AsNOS), which limits parasite development and which in turn ... MEK and ERK. Computational models linking TGF-β1, AsNOS, and MEK/ERK were developed to provide insights ... as occurs in mammalian cells, MEK/ERK and AsNOS would oscillate upon ingestion of TGF-β1. An ... β1-induced multiphasic behavior of MEK/ERK and AsNOS. To achieve this multiphasic behavior, the ODE model ... As60A expression together may act to maintain multiphasic AsNOS expression via MEK/ERK-dependent signaling. We hypothesize ...
Tópico(s): Mosquito-borne diseases and control
2013 - Elsevier BV | Journal of Theoretical Biology
Ryotaro Hara, Masashi Nakano, Kuniki Kino,
... hydrolase. A well-characterized mutant of asparagine hydroxylase (AsnO-D241N) and its homologous enzyme (SCO2693-D246N) were ... highly stable and efficient wild-type asparagine hydroxylases AsnO and SCO2693 were employed to synthesize L-THA. ... THA was obtained by L-asparagine hydroxylation by AsnO followed by amide hydrolysis by asparaginase via 3- ... 076% by using intact Escherichia coli expressing the asnO gene, and thus, two asparaginase-deficient mutants of ... deficient mutant. When the expression level of the asnO gene was enhanced by using the T7 promoter ...
Tópico(s): Metabolism and Genetic Disorders
2015 - American Society for Microbiology | Applied and Environmental Microbiology
Allan C. Baxter, David R. Williams,
Thermodynamic-based structures are suggested for [Cu(AsnO)(HisO)], [Cu(AsnO)(HHisO)]+, [Cu(AsnO)-(ThrO)], [Cu(HisO)(ThrO)], and [Cu(HHisO)(ThrO)]+ in aqueous solution at 25 °C and I= 3. ... tridentate to CuII, threoninate (ThrO) bidentate, and asparaginate (AsnO) ambivalent. The site of proton attachment in the ...
Tópico(s): Lanthanide and Transition Metal Complexes
1975 - Royal Society of Chemistry | Journal of the Chemical Society. Dalton transactions
Shirley Luckhart, Matthew J. Lieber, Naresh Singh, Rubén Zamora, Yoram Vodovotz,
... also regulates parasite development with inducible NO synthase (AsNOS). We show that exogenous human TGF-β1 dose-dependently regulates mosquito AsNOS expression and that parasite killing by low dose TGF-β1 depends on AsNOS catalysis. Furthermore, induction of AsNOS expression by TGF-β1 is regulated by NO ...
Tópico(s): Insect Resistance and Genetics
2007 - Elsevier BV | Experimental Parasitology
Shirley Luckhart, Kai-Xuan Li,
Anopheles stephensi nitric oxide synthase (AsNOS) is a single copy gene that shares significant structural homology with the three human NOS genes and is inducibly expressed in ... from this gene. A total of 18–22 AsNOS transcripts, ranging in size from 1.0 to ... sequence for this exon. The large number of AsNOS transcripts and diversity in AsNOS splicing and exon 1 sequences indicate that transcriptional ...
Tópico(s): Physiological and biochemical adaptations
2001 - Elsevier BV | Insect Biochemistry and Molecular Biology
Yuri Stasiv, Michael Regulski, Kuzin Ba, Tim Tully, Grigori Enikolopov,
... well as with the mosquito Anopheles stephensi NOS(AsNOS) gene (17Hall A.V. Antoniou H. Wang Y. ... have the same size as the homologous mosquito AsNOS exons (exons 2–5, 7, 10, and 11, ... for the human nNOS and for the mosquito AsNOS genes. Alternatively spliced exons not present in the ... for the human nNOS and for the mosquito AsNOS genes. Alternatively spliced exons not present in the ...
Tópico(s): Renin-Angiotensin System Studies
2001 - Elsevier BV | Journal of Biological Chemistry
Joanne M. Neary, Amanda Powell, Lyndsey Gordon, Claire Milne, Fiona Flett, Barrie Wilkinson, Colin P. Smith, Jason Micklefield,
... and 3-hydroxyasparaginyl phosphotransferase-encoding genes hasP and asnO were separately deleted from the calcium-dependent antibiotic ( ... retain calcium-dependent antimicrobial activity. This confirms that AsnO and HasP are required for the beta-hydroxylation ...
Tópico(s): Carbohydrate Chemistry and Synthesis
2007 - Microbiology Society | Microbiology
... 1st Congress of the Asian Neuro-ophthalmology Society (ASNOS) was presided over by Dr Akio Tagbuchi, and ... in Tokyo for the 16th INOS meeting, 3rd ASNOS meeting, and 44th JNOS meeting. Notably, a program ... time. It is now a part of every ASNOS meeting. Currently, there are 964 members of JNOS, ...
Tópico(s): Medical and Biological Sciences
2012 - Lippincott Williams & Wilkins | Journal of Neuro-Ophthalmology
... to Safeguards Development’, Australian Safeguards and Nonproliferation Office (ASNO), Barton, Australia, 2007 < http://www.dfat.gov.au/asno/publications/SAGSI_role_contribution_safeguards_dev.pdf>.10 ... Florida, July 2004 < http://www.dfat.gov.au/asno/publications/inmm2004_information.pdf>.11 These tensions were ...
Tópico(s): Nuclear Issues and Defense
2014 - Taylor & Francis | Intelligence & National Security
Jan A. C. Vriezen, Frans J. de Bruijn, Klaus Nüsslein,
... desiccation. This approach enabled identification of the loci asnO and ngg by their reduced ability to grow ... sensitivity of the mutant to β-lactam antibiotics. asnO::Tn5luxAB is expressed during the stationary phase under ... resistance to β-lactam antibiotics may indicate that asnO is involved in the production of a β- ...
Tópico(s): Plant nutrient uptake and metabolism
2013 - American Society for Microbiology | Applied and Environmental Microbiology
Juan David Montoya Páez, Benjamín Alberto Rojano, Giovanni Restrepo Betancur,
La congelación de semen de asno permite almacenar material genético por un periodo ilimitado de tiempo, por lo que puede ser empleado para generar bancos de germoplasma o para la cría de mulares y asnos. Esta investigación tuvo como objetivo evaluar alternativas de ... un diluyente para la congelación de semen de asno. Se colectó el semen de cinco asnos criollos colombianos, dos eyaculados por animal (n= 10) ... mejorar la calidad post-descongelación del semen de asno.
Tópico(s): Seed Germination and Physiology
2017 - University of Córdoba | Archivos de Zootecnia
Shirley Luckhart, Andrea Crampton, Rubén Zamora, Matthew J. Lieber, Patricia C. Dos Santos, Tina M.L. Peterson, Nicole Emmith, Jung-Hwa Lim, David A. Wink, Yoram Vodovotz,
... l- arginine oxidation by A. stephensi NO synthase (AsNOS). Active TGF-β1 persists in the mosquito midgut ... in mammals by TGF-β1, TGF-β1 regulates AsNOS expression and Plasmodium development in A. stephensi . Together, ...
Tópico(s): Aquaculture Nutrition and Growth
2003 - American Society for Microbiology | Infection and Immunity
... de un total de 275 caballos y 2 asnos. Según el método del mezclador estaban afectados 89 caballos (32,4 %) y 1 asno. Los utrículos de Miescher de los équidos son ...
Tópico(s): Coccidia and coccidiosis research
2010 - Wiley | Zentralblatt für Veterinärmedizin Reihe B
Leyla Akman-Anderson, Martin Olivier, Shirley Luckhart,
... glycosylphosphatidylinositols (PfGPIs) can induce A. stephensi NO synthase (AsNOS) expression in the midgut epithelium in vivo in ... study, we demonstrate that Hz can also induce AsNOS gene expression in immortalized A. stephensi and Anopheles ...
Tópico(s): Mosquito-borne diseases and control
2007 - American Society for Microbiology | Infection and Immunity
Jung-Hwa Lim, D. Channe Gowda, Krishne Gowda, Shirley Luckhart,
... significant expression of A. stephensi nitric oxide synthase (AsNOS) in the midgut epithelium as early as 6 ... this study, we demonstrate that PfGPIs can induce AsNOS expression in A. stephensi cells in vitro and ...
Tópico(s): Parasites and Host Interactions
2005 - American Society for Microbiology | Infection and Immunity
Yoram Vodovotz, Rubén Zamora, Matthew J. Lieber, Shirley Luckhart,
... action of an inducible Anopheles stephensi NO synthase (AsNOS) may be central to the anti-parasitic arsenal ... in the insect midgut, and induction of both AsNOS and TGF-β-like (As60A) gene expression in ...
Tópico(s): Renin-Angiotensin System Studies
2004 - Bentham Science Publishers | Current Molecular Medicine
Mohammed A. Elmakki, Orbett T. Alexander, Johan A. Venter, Andreas Roodt,
Abstract C 29 H 23 AsNO 2 ${\text{C}}_{29}{\text{H}}_{23}{\text{AsNO}}_{2}$ Rh, triclinic, P 1 ‾ $P‾{1}$ (no. 2), a = 9. ...
Tópico(s): Organoboron and organosilicon chemistry
2020 - De Gruyter | Zeitschrift für Kristallographie - New Crystal Structures
P. de Meester, Shirley S. Chu, Misa V. Jovanovic, Edward R. Biehl,
... phenyl‐5,10‐dihydrophenarsazine, C 19 H 16 AsNO has been determined by the single crystal x‐ ...
Tópico(s): Synthesis and biological activity
1985 - Wiley | Journal of Heterocyclic Chemistry
Mohammed A. Elmakki, Orbett T. Alexander, Johan A. Venter, Andreas Roodt,
Abstract C 24 H 19 AsNO 3 Rh, triclinic, P 1 ‾ $‾{1}$ (no. 2), a = 9.1358(5) Å, b = 9.6478(6) Å, c = 12. ...
Tópico(s): Organometallic Complex Synthesis and Catalysis
2020 - De Gruyter | Zeitschrift für Kristallographie - New Crystal Structures
V. I. Bragin, J. D. Carlson, Russell Leslie,
... at the Australian Safeguards and Non-Proliferation Office (ASNO) and Coordinator of the Australian Safeguards Support Program. Mr. John Carlson is Director-General of ASNO and member of the Standing Advisory Group on ... Implementation. Mr. Russell Leslie is Safeguards Adviser at ASNO. All three authors have made substantial contributions to ...
Tópico(s): Nuclear and radioactivity studies
2001 - Taylor & Francis | The Nonproliferation Review
John Beusterien, J. Baird Callicott,
... que nunca Sancho Panza se apartó de su asno, ni su asno de Sancho Panza” (965) (“They'll take my ... hizo lugar donde con facilidad pudiese entrar el asno, como lo hizo. (966)(And then Sancho discovered ...
Tópico(s): Seventeenth-Century Political and Philosophical Thought
2013 - Penn State University Press | Comparative Literature Studies
Veronica J. Dark, Geoffrey R. Loftus,
... the other half of the trials (referred to asno-recall trials). Results were consistent over the experiments: ...
Tópico(s): Neural and Behavioral Psychology Studies
1976 - Academic Press | Journal of Verbal Learning and Verbal Behavior
... ocular se estudiaron en los siguientes animales: caballo, asno, nula, vaca, búfalo, camello, oveja, cabra, cerdo, perro ...
Tópico(s): Facial Rejuvenation and Surgery Techniques
1982 - Wiley | Anatomia Histologia Embryologia