Y.S.P. Alvares, M. José Alves, Nuno G. Azóia, J.F. Bickley, T. L. Gilchrist,
Reaction of the chiral azirine 1a with nucleophiles and dienes is described. Thiols, heteroaromatic nitrogen compounds and phenylmagnesium bromide add to the azirine 1a to give functionalised aziridines 2/3a–h. X-Ray crystal structures for the products 3e and 3g have been obtained. Diastereodifferentiation of the two faces of the azirine 1a is observed in most cases, but only thiophenol gives a single diastereomer (2a). Most mixtures of diastereomers were separated by dry flash chromatography. Benzylamine ...
Tópico(s): Catalytic C–H Functionalization Methods
2002 - Royal Society of Chemistry | Journal of the Chemical Society Perkin Transactions 1
... a limestone cliff hanging over the sea at Azóia, Sesimbra, around 40 km south of Lisbon. This ...
Tópico(s): Paleopathology and ancient diseases
2010 - | Anthropological Science
M. José Alves, Nuno G. Azóia, A. Gil Fortes,
Cycloadditions of the glyoxylate imine 1 with 1-substituted and 1,4-disubstituted 1,3-dienes furnished tetrahydroquinoline compounds 4 and 5/15 with total regio- and stereo-control, except for one case where a mixture of isomers was formed. A stepwise mechanism is proposed in view of the stereochemistry of the products.
Tópico(s): Oxidative Organic Chemistry Reactions
2006 - Elsevier BV | Tetrahedron
Raquel Silva, Helena Ferreira, Nuno G. Azóia, Ulyana Shimanovich, Giuliano Freddi, Aharon Gedanken, Artur Cavaco‐Paulo,
Microspheres of bovine serum albumin (BSA) and silk fibroin are produced by applying ultrasound in a biphasic system consisting of an aqueous protein solution and an organic solvent. The protein microspheres are dispersed in an aqueous media where the protein remains at the interface covering the organic solvent. This only occurs when high shear forces are applied that induce changes to force the protein to the interface. Fourier transform infrared results indicate a large increase in the content ...
Tópico(s): Proteins in Food Systems
2012 - American Chemical Society | Molecular Pharmaceutics
Madalena Martins, Nuno G. Azóia, Ulyana Shimanovich, Teresa Matamá, Andreia C. Gomes, Carla Silva, Artur Cavaco‐Paulo,
A novel transdermal hyaluronic acid (HA) conjugated with bovine serum albumin (BSA) was developed in the form of solid-in-oil (S/O) nanodispersion (129.7 nm mean diameter). Ex vivo skin penetration analysis by fluorescence and confocal observation of histological skin sections revealed the ability of BSA/HA nanodispersions to cross the stratum corneum and penetrate into the dermis. Furthermore, no significant toxicity was found in fibroblast and keratinocyte cells in vitro. These results proved the ...
Tópico(s): Advanced Drug Delivery Systems
2014 - American Chemical Society | Molecular Pharmaceutics
Carla Silva, Teresa Matamá, Nuno G. Azóia, Catarina Mansilha, Margarida Casal, Artur Cavaco‐Paulo,
The application of an odorant binding protein for odour control and fragrance delayed release from a textile surface was first explored in this work. Pig OBP-1 gene was cloned and expressed in Escherichia coli, and the purified protein was biochemically characterized. The IC50 values (concentrations of competitor that caused a decay of fluorescence to half-maximal intensity) were determined for four distinct fragrances, namely, citronellol, benzyl benzoate, citronellyl valerate and ethyl valerate. ...
Tópico(s): Biochemical Analysis and Sensing Techniques
2013 - Springer Science+Business Media | Applied Microbiology and Biotechnology
Madalena Martins, Nuno G. Azóia, Artur Ribeiro, Ulyana Shimanovich, Carla Silva, Artur Cavaco‐Paulo,
Transdermal perfusion of a large protein is reported for the first time, using a nanoemulsion of bovine serum albumin (66 kDa) of 160 nm prepared by a solid-in-oil (S/O) process. Molecular dynamics simulations confirmed skin permeation by these formulations, with integration of the protein into the lipid bilayers. These results demonstrate the real possibility of delivering large proteins transdermally for a range of medical and cosmetic applications.
Tópico(s): Proteins in Food Systems
2013 - Elsevier BV | Colloids and Surfaces B Biointerfaces
Célia F. Cruz, Margarida M. Fernandes, Andreia C. Gomes, L. Coderch, Meritxell Martı́, Sialia Karina Mellink-Méndez, Luı́s Gales, Nuno G. Azóia, Ulyana Shimanovich, Artur Cavaco‐Paulo,
Synopsis Human hair has an important and undeniable relevance in society due to its important role in visual appearance and social communication. Hair is mainly composed of structural proteins, mainly keratin and keratin associated proteins and lipids. Herein, we report a comprehensive study of the content and distribution of the lipids among ethnic hair, African, Asian and Caucasian hair. More interestingly, we also report the study of the interaction between those two main components of hair, ...
Tópico(s): Skin and Cellular Biology Research
2013 - Wiley | International Journal of Cosmetic Science
Daniel A. Bercovici, Jodi M. Ogilvie, Nikolay P. Tsvetkov, Matthias Brewer,
... 5269– 5278. 3 3aM. J. Alves, N. G. Azoia, A. G. Fortes, Tetrahedron 2007, 63, 727– 734; ...
Tópico(s): Organic Chemistry Cycloaddition Reactions
2013 - Wiley | Angewandte Chemie International Edition
M. José Alves, Nuno G. Azóia, J.F. Bickley, A. Gil Fortes, T. L. Gilchrist, Ricardo Mendonça,
Methyl 2-(2,6-dichlorophenyl)-2H-azirine-3-carboxylate 1 and furan give the aziridine 2 by a Diels–Alder cycloaddition reaction. The hydrolysis of compound 2 leads to a dihydrofuranol 11 by cleavage of a C–N bond. X-Ray crystal structures of compounds 2 and 11 have been determined. Compound 2 reacts with alcohols in a similar way to give 2-alkoxy-2,5-dihydrofurans as mixtures of cis and trans isomers. The structures of these compounds have been determined from an X-ray crystal structure of one of ...
Tópico(s): Organic Chemistry Cycloaddition Reactions
2001 - Royal Society of Chemistry | Journal of the Chemical Society Perkin Transactions 1
Ana Loureiro, Eugénia Nogueira, Nuno G. Azóia, Marisa P. Sárria, Ana S. Abreu, Ulyana Shimanovich, Alexandra Rollett, Johan Härmark, Hans Hebert, Georg M. Guebitz, Gonçalo J. L. Bernardes, Ana Preto, Andreia C. Gomes, Artur Cavaco‐Paulo,
Bovine serum albumin (BSA) nanoemulsions were produced by high pressure homogenization with a tri-block copolymer (Poloxamer 407), which presents a central hydrophobic chain of polyoxypropylene (PPO) and two identical lateral hydrophilic chains of polyethylene glycol (PEG). We observed a linear correlation between tri-block copolymer concentration and size - the use of 5mg/mL of Poloxamer 407 yields nanoemulsions smaller than 100nm. Molecular dynamics and fluorescent tagging of the tri-block copolymer ...
Tópico(s): Proteins in Food Systems
2015 - Elsevier BV | Colloids and Surfaces B Biointerfaces
Eugénia Nogueira, Irene C. Mangialavori, Ana Loureiro, Nuno G. Azóia, Marisa P. Sárria, Patrícia Nogueira, Jaime Freitas, Johan Härmark, Ulyana Shimanovich, Alexandra Rollett, Ghislaine Lacroix, Gonçalo J. L. Bernardes, Georg M. Guebitz, Hans Hebert, Alexandra Moreira, Alexandre M. Carmo, Juan Pablo F.C. Rossi, Andreia C. Gomes, Ana Preto, Artur Cavaco‐Paulo,
Specific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. Here, we report an innovative strategy for targeted liposome delivery that uses a hydrophobic fragment of surfactant protein D linked to folate. Our proposed spacer is a small 4 amino ...
Tópico(s): Advanced biosensing and bioanalysis techniques
2015 - American Chemical Society | Biomacromolecules
Egipto Antunes, Célia F. Cruz, Nuno G. Azóia, Artur Cavaco‐Paulo,
Molecular dynamics simulations with a developed hair protofibril model demonstrated the ability to improve peptide uptake by hair shafts.
Tópico(s): Silk-based biomaterials and applications
2015 - Royal Society of Chemistry | RSC Advances
Produção Nacional
Revisado por pares
Laurita dos Santos, Claudio A. Téllez S., Mariane P. J. Sousa, Nuno G. Azóia, Artur Cavaco‐Paulo, Aírton Abrahão Martin, Priscila P. Fávero,
The purpose of this study is to elucidate the behavior of retinyl acetate in penetrating human skin without the presence of enhancers by using confocal Raman spectroscopy and molecular dynamics simulation. In this study, in vivo confocal Raman spectroscopy was combined with molecular dynamics simulation to investigate the transdermal permeation of the aqueous suspension of retinyl acetate. Permeation was measured after 30 min, and retinyl acetate was found up to 20 μm deep inside the stratum corneum. ...
Tópico(s): Surfactants and Colloidal Systems
2016 - Elsevier BV | Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy
Egipto Antunes, Célia F. Cruz, Nuno G. Azóia, Artur Cavaco‐Paulo,
A computational molecular model of a truncated keratin protofibril (8 chains of hair keratin, PDB provided in Supplementary material) was used, to run a series of steered molecular dynamics simulations obtaining strain-stress curves. These results were compared with experimental mechanical data on hair fibers. Our data demonstrate that the molecular dynamics simulations can model hair mechanical properties. Simulations done in vacuum showed a better agreement with experimental Young's Modulus (YM) ...
Tópico(s): Skin and Cellular Biology Research
2016 - Elsevier BV | International Journal of Biological Macromolecules
Ana Loureiro, Nuno G. Azóia, Andreia C. Gomes, Artur Cavaco‐Paulo,
Nanomedicine, the application of nanotechnology to medicine, is being increasingly used to improve and exploit the advantages of efficient drug delivery. Different nanodevices have been developed in recent years, among them protein-based nanoparticles which have gained considerable interest. Albumin is a versatile protein carrier with several characteristics that make it an ideal candidate for drug delivery, such as its availability, its biocompatibility, its biodegradability, and its lack of toxicity ...
Tópico(s): Nanocluster Synthesis and Applications
2016 - Bentham Science Publishers | Current Pharmaceutical Design
Madalena Martins, Ana Loureiro, Nuno G. Azóia, Carla Silva, Artur Cavaco‐Paulo,
Needs from medical and cosmetic areas have led to the design of novel nanosized emulsions and solid-in-oil dispersions of proteins. Here, we describe the production of those emulsions and dispersions using high-energy methodologies such as high-pressure homogenization or ultrasound. Recent work has resulted in new mechanistic insights related to the formation of protein emulsions and dispersions. The production method and composition of these formulations can determine major parameters such as size, ...
Tópico(s): Lipid Membrane Structure and Behavior
2016 - Elsevier BV | Trends in biotechnology
Célia F. Cruz, Nuno G. Azóia, Teresa Matamá, Artur Cavaco‐Paulo,
We selected 1235 decapeptides from human hair proteins encoded by human genes of keratins and keratin associated proteins. The peptides were linked to glass arrays and screened for their affinity towards a solution of human hair extracted keratin fraction. Based on the physicochemical properties of the peptides, ten variables were studied: content of different types of amino acid side chains (cysteine, hydrophobic, polar, basic, acidic, aromatic rings, amide, alcohol side chains), isoelectric point, ...
Tópico(s): Skin Protection and Aging
2017 - Elsevier BV | International Journal of Biological Macromolecules
Jing Su, Jennifer Noro, Ana Loureiro, Madalena Martins, Nuno G. Azóia, Jiajia Fu, Qiang Wang, Carla Silva, Artur Cavaco‐Paulo,
Abstract Laccase catalyzes the oxidation and polymerization of phenolic compounds in the presence of oxygen. Herein, we report for the first time that a previous PEGylation of laccase enhances the polymerase activity 3‐fold compared with the reaction of the native enzyme, as confirmed by UV/Vis spectroscopy. The polymerization of catechol increased only 1.5‐fold if poly(ethylene glycol) (PEG) was added to the medium reaction. Molecular‐dynamics simulations suggest the formation of a miscible complex ...
Tópico(s): Electrochemical sensors and biosensors
2017 - Wiley | ChemCatChem
Produção Nacional
Revisado por pares
Helenice Maria Sacht, Marieli Azoia Lukiantchuki,
Abstract Natural ventilation is efficient for passive cooling of buildings, to improve indoor air quality, occupant comfort and, consequently, the reduction of the use of electric energy. Characteristics such as the type of windows, if fixed or mobile; effective ventilation area; operation; if present elements that offer greater resistance to the passage of the air, it influences directly on the conditions of natural ventilation. Therefore, the present work aimed to compare the natural ventilation ...
Tópico(s): Infection Control and Ventilation
2017 - Elsevier BV | Procedia Engineering
Eugénia Nogueira, Marisa P. Sárria, Nuno G. Azóia, Egipto Antunes, Ana Loureiro, Diana Guimarães, Jennifer Noro, Alexandra Rollett, Georg M. Guebitz, Artur Cavaco‐Paulo,
The folate antagonist methotrexate is a cytotoxic drug used in the treatment of several cancer types. The entry of methotrexate into the cell is mediated by two main transport systems: the reduced folate carrier and membrane-associated folate receptors. These transporters differ considerably in their mechanism of (anti)folate uptake, substrate specificity, and tissue specificity. Although the mechanism of action of the reduced folate carrier is fairly well-established, that of the folate receptor ...
Tópico(s): Bone and Joint Diseases
2018 - American Chemical Society | Biochemistry
Sonia Labeau, Elsa Afonso, Julie Benbenishty, Bronagh Blackwood, Carole Boulanger, Stephen J. Brett, Silvia Calviño-Günther, Wendy Chaboyer, Fiona Coyer, Mieke Deschepper, Guy François, Patrick M. Honorè, Radmilo Janković, Ashish K. Khanna, Mireia Llauradó‐Serra, Frances Lin, Louise Rose, Francesca Rubulotta, Leif Saager, Ged Williams, Stijn Blot, Dritan Muzha, Antoni Margarit Ribas, Fernando Lipovesty, Cecilia Loudet, Fiona Coyer, Philipp Eller, Nafseen Mostafa, Patrick M. Honorè, Vanesa Mercado Telleria, Jasmina Smajić, Paula Cristina Nogueira, Khalid Mahmood Khan Nafees, Romuald Hentchoya, Louise Rose, Javiera Soledad, Frances Lin, Yenny Cardenas, A. Reyes, Alan S̆ustić, Meropi Mpouzika, Tamas Vymazal, Hanne Irene Jensen, Hernán Aguirre-Bermeo, Liivi Maddison, M. Valta, Silvia Calviño-Günther, Frank Bloos, Faustina Excel Adipa, Vasilios Koulouras, Judy Enamorado Enamorado, Zsuzsann Ágoston, Hrönn Birgisdóttir, Amit Gupta, Mohan Gurjar, Bram Kilapong, Seyed Mohammad Reza Hashemian, Ignacio Martín‐Loeches, Julie Benbenishty, Andrea Cortegiani, Kelly Fletcher, Yoshiro Hayashi, Wangari Waweru-Siika, Khalid Abidi, Sang‐Min Lee, B. Hadri, Mihails Dolgusevs, Fayez Abillama, Tomas Jovaiša, Cyril A Thix, Muhammed Elhadi, Mohd Basri Mat Nor, Shanti Ratnam, Mohd Zulfakar Mazlan, Sundaresan Maiyalagan, Luis Alejandro Sánchez‐Hurtado, Adrian Belii, Mendsaikhan Naranpurev, Prabha Gautam, Dylan W. de Lange, Rachael Parke, Rose Ilesanmi, Mirjana Shosholcheva, Antonija Petosic, Ranveig Lind, Madiha Hashmi Ffarcsi, Javier Bogarin, Aaron Mark Hernandez, Małgorzata Mikaszewska-Sokolewicz, B. A. M. Sousa, Dana Tomescu, Dorel Săndesc, Théogène Twagirumugabe, V. G. Gusarov, Maie Salem Ebaid, Radmilo Janković, Gari Slobodianiuk, Andrea Martonova, Rihard Knafelj, Mervyn Mer, Emilio Maseda, Bernardo Panka, Joerg C. Schefold, Eva Joelsson‐Alm, Konlawij Trongtrakul, Lorna Merritt-Charles, Lamia Ouanes-Besbes, Yalım Dikmen, Л. В. Згржебловська, Mark Fielding, Francesca Rubulotta, Ashish K. Khanna, Leif Saager, Ingrid von der Osten, Dritan Muzha, Alban Greca, Alma Cani, Nordian Xhindi, Genci Hyska, Antonio Margarit Ribas, Susana Pinto-Castilla, Paulo Alves, Romina Esposito, Emanuel Valgolio, John Thomas Sanchez Miñope, Antonio Abdala, Maria Ayala, Silvina Bravo, Ana Bantar, Patricia Delgado, Gustavo Badariotti, Fernando Lipovestky, Ana María Díaz, Pablo Saul, Mariano Setten, Alejandra Aucapina, Ysica Acosta, V. González, Luis Alberto Camputaro, Fernando Baccaro, Robert Villa, Ana María Díaz, Marcela Mastantuono, Emiliano Dean, Oscar Fernández Rostello, Patricia Brizuela, Julio Ricardo Bartoli, Matias Guereschi, Cristian Quiroga, Sofia Putruele, Paula Romina Villegas, Veronica Curilen, Rubén Fernández, Mariangeles Gabriela Nocheretti, Rosana Gabriela Escalante, Cecilia Loudet, Silvia Fernandez, Ana Laura González, Gustavo Andres Alvarez, Federico Iglesias, S. Jansen Chaparro, Graciela Zakalik, Gonzalo Pagella, Matías Baini, Pierina Arias Campos, Ignacio Sabbag, Armando Schmukler, Imelda Perdomo Fonseca, Gonzalo Martín Alvarez, Mário Ramirez, Fernando Velasco-Tapia, Carlos Alejandro Bascary, G. Giménez, Fernando Bertoletti, Esteban Milioto, Pablo Julio Maldonaldo Bonsignore, María Alejandra Fernández, Julie Smith, Tim Chimunda, Lorraine Thompson, Teena Maguire, Frances Lin, Wendy Chaboyer, Fiona Coyer, Stacey Watts, Marion Mitchell, Madeleine Powell, India Lye, Leanne Parsons, Nerilee Baker, Claire Reynolds, Amy Thompson, Kristy Masters, Kellie Sosnowski, Lynette Morrison, Gavin Leslie, Ramanathan Lakshmanan, Alexis Tabah, Wendy Brown, Sharon McDowell-Skaines, Andrea McLucas, Chris Smith, Mandy Tallot, Sarah Jones, Michelle Barakat‐Johnson, Thomas K. Leong, Rand Butcher, Kerrie Martin, Philipp Douschan, Dirk von Lewinski, Philipp Eller, René Schmutz, Uta Kolussi, Fatema Salman, Zainab Mahdi Ateya, Nafseen Mostafa, Koen De Decker, Niels Van Regenmortel, Anita Jans, Patricia Wijnands, Stefano Coremans, Patrick M. Honorè, David De Bels, Tanja Depuydt, Caroline Paillet, Luc‐Marie Jacquet, Walter Swinnen, Francis Hannes, M Mergeay, Stijn Van de Velde, Silvie Allaert, Pieter Hoste, Christophe Borin, Sandrine Balon, Vincent Fraipont, Patrick Biston, Nicolas De Schryver, Thierry Dugernier, Ilse Van Cotthem, Vanesa Mercado Telleria, Jasmina Smajić, Angélica Olivetto de Almeida, Sílvia Angélica Jorge, Delmiro Becker, Raysa Cristina Schmidt, Evellyn Oliveira, Aline de Oliveira Ramalho, Eliane Mazócoli, Audrey Fioretti, Elaine Barros, Letícia Faria Serpa, Suzana Maria Bianchini, Ticiane Carolina Gonçalves Faustino Campanili, Taís Pantaleao, Paulo Carlos Garcia, Ana Lucia Vitti Ronchini, Rayanne Santos, Khalid Mahmood Khan Nafees, Nurulhuda Binti A. Manap, Romuald Hentchoya, Sean M. Bagshaw, Dominic Carney, Sean M. Bagshaw, Jon Davidow, Sean M. Bagshaw, Ella Rokosh, Sean M. Bagshaw, Andréa Maria Laizner, Samantha Smith, Megan McQuirter, Betty Star Kampayana, René Favre, Martin Sills, Andréa Maria Laizner, Julie Dallaire, Andréa Maria Laizner, Cathy Becker, Sherissa Microys, B. Bowes, Jennifer LaJeunesse, Rishi Ghosh, Jacqueline Baptiste-Savoie, Rose Raizman, Sean M. Bagshaw, Gabriel Suen, Noushin Taghavi, Orla Smith, Clare Fielding, Louise Rose, J R López Canales, Pia Molina, Javiera Chaparro, Maria Idalia Sepulveda, Matias Jesús Flamm Zamorano, P..c. Túlio Monteiro de Castro e Abreu Rocha, Ximena Villanueva,
Intensive care unit (ICU) patients are particularly susceptible to developing pressure injuries. Epidemiologic data is however unavailable. We aimed to provide an international picture of the extent of pressure injuries and factors associated with ICU-acquired pressure injuries in adult ICU patients. International 1-day point-prevalence study; follow-up for outcome assessment until hospital discharge (maximum 12 weeks). Factors associated with ICU-acquired pressure injury and hospital mortality were ...
Springer Science+Business Media
Catarina Costa, Nuno G. Azóia, Carla Silva, Eduardo F. Marques,
In the last years, sustainable practices have been developed to minimize the negative effects of production and excessive consumption on the environment. The textile and clothing industry is one of the most polluting industries globally and needs to rethink its strategies. The fast-fashion caused an increase in production, and the environmental weight associated with the textile industry also increased. The problems range from the enormous expenditure of water resources to the carbon and greenhouse ...
Tópico(s): Dyeing and Modifying Textile Fibers
2020 - University of Porto | U Porto Journal of Engineering
Tuğçe Çaykara, Maria G. Sande, Nuno G. Azóia, L. R. Rodrigues, Carla Silva,
Abstract Polyethylene terephthalate (PET) is one of the most used polymeric materials in the health care sector mainly due to its advantages that include biocompatibility, high uniformity, mechanical strength and resistance against chemicals and/or abrasion. However, avoiding bacterial contamination on PET is still an unsolved challenge and two main strategies are being explored to overcome this drawback: the anti-adhesive and biocidal modification of PET surface. While bacterial adhesion depends ...
Tópico(s): Dental materials and restorations
2020 - Springer Science+Business Media | Medical Microbiology and Immunology
Catarina Costa, Nuno G. Azóia, Lorena Coelho, Ricardo Freixo, Patrícia Batista, Manuela Pintado,
The disposal of a high volume of waste-containing proteins is becoming increasingly challenging in a society that is aware of what is happening in the environment. The dairy industry generates several by-products that contain vast amounts of compounds, including proteins that are of industrial importance and for which new uses are being sought. This article provides a comprehensive review of the potential of the valorisation of proteins that can be recovered by chemical and/or physical processes ...
Tópico(s): Meat and Animal Product Quality
2021 - Multidisciplinary Digital Publishing Institute | Foods
Catarina Felgueiras, Nuno G. Azóia, Cidália Maria Abreu Gonçalves, Miguel Gama, Fernando Dourado,
There is an emerging environmental awareness and social concern regarding the environmental impact of the textile industry, highlighting the growing need for developing green and sustainable approaches throughout this industry’s supply chain. Upstream, due to population growth and the rise in consumption of textile fibers, new sustainable raw materials and processes must be found. Cellulose presents unique structural features, being the most important and available renewable resource for textiles. ...
Tópico(s): Electrospun Nanofibers in Biomedical Applications
2021 - Frontiers Media | Frontiers in Bioengineering and Biotechnology
Catarina Costa, André Viana, Carla Silva, Eduardo F. Marques, Nuno G. Azóia,
Chemical recycling can be used to separate fibers that are constituents of different types of fabrics. This type of process can be considered one of the most effective forms of recycling, given that a large part of fabrics is made up of fiber mixtures. As part of an innovative circular strategy, the main goal of this work was to study the conditions for extracting cellulose from mixed textile wastes by acid hydrolysis and further transform it into cellulose derivatives, thus contributing to reduce ...
Tópico(s): Dyeing and Modifying Textile Fibers
2022 - Elsevier BV | Waste Management
Andreia Vasconcelos, Nuno G. Azóia, Ana C. Carvalho, Andreia C. Gomes, Georg M. Guebitz, Artur Cavaco‐Paulo,
Chronic wounds are the result of excessive amounts of tissue destructive proteases such as human neutrophil elastase (HNE). The high levels of this enzyme found on those types of wounds inactivate the endogenous inhibitor barrier thus, the search for new HNE inhibitors is required. This work presents two new HNE inhibitor peptides, which were synthesized based on the reactive-site loop of the Bowman–Birk inhibitor protein. The results obtained indicated that these new peptides are competitive inhibitors ...
Tópico(s): Protease and Inhibitor Mechanisms
2011 - Elsevier BV | European Journal of Pharmacology
Nuno G. Azóia, Margarida M. Fernandes, Nuno M. Micaêlo, Cláudio M. Soares, Artur Cavaco‐Paulo,
Molecular dynamics simulations of a keratin/peptide complex have been conducted to predict the binding affinity of four different peptides toward human hair. Free energy calculations on the peptides' interaction with the keratin model demonstrated that electrostatic interactions are believed to be the main driving force stabilizing the complex. The molecular mechanics-Poisson-Boltzmann surface area methodology used for the free energy calculations demonstrated that the dielectric constant in the ...
Tópico(s): Hair Growth and Disorders
2012 - Wiley | Proteins Structure Function and Bioinformatics
Produção Nacional
Revisado por pares
Neila Machado, Laurita dos Santos, Bruna G. Carvalho, Priyanka Singh, Claudio A. Téllez Soto, Nuno G. Azóia, Artur Cavaco‐Paulo, Aírton Abrahão Martin, Priscila P. Fávero,
The present work, involves the simulation of the transport of a vitamin C derivative, Ascorbyl Tetraisopalmitate (ATI), through human skin by molecular dynamics. Percutaneous absorption of the ATI molecule through the infundibulum, an important route of absorption into the hair follicle of the human skin, has been modeled and compared with the stratum corneum membrane. The comparative study was done using molecular dynamics with Martini force field. In infundibulum, a single ATI molecule require ...
Tópico(s): Dermatology and Skin Diseases
2016 - Elsevier BV | Computers in Biology and Medicine