... Cheshire, Cumberland, Derbyshire, Devon, Gloucestershire. Letter. From Edw Bama Rd. To Lord John Russell.
0000 - Gale Group | NCCO BritishPolitics
Drew Bennion, Emily S. Charlson, Eric R. Coon, Rajeev Misra,
BamA of Escherichia coli is an essential component of the hetero-oligomeric machinery that mediates β-barrel ... OMP) assembly. The C- and N-termini of BamA fold into trans-membrane β-barrel and five soluble POTRA domains respectively. Detailed characterization of BamA POTRA 1 missense and deletion mutants revealed two ... on POTRA 1. Interestingly, our data suggest that BamA also requires its POTRA 1 domain for proper ... analysis revealed that the POTRA 1 domain of BamA interacts with SurA, a periplasmic chaperone required for ...
Tópico(s): Cellular transport and secretion
2010 - Wiley | Molecular Microbiology
... and Others, Anonymous. Rewab Chiefs, Mauyusia Raja of Bama, Raja of.
0000 - Gale Group | NCCO Photography
U. Lehr, Monika Schütz, Philipp Oberhettinger, Fernando Ruı́z-Pérez, James W. Donald, Tracy Palmer, Dirk Linke, Ian R. Henderson, Ingo B. Autenrieth,
... is composed of the essential outer membrane protein BamA and four outer membrane associated lipoproteins BamB-E. ... barrel membrane anchor. To investigate the role of BamA in biogenesis of TAAs, we expressed YadA in a BamA-depleted strain of Escherichia coli, which resulted in ... two-hybrid experiments and immunofluorescence studies revealed that BamA and YadA interact directly and colocalize. As BamA recognizes the C-terminus of OMPs, we exchanged ... outer membrane. In summary we demonstrate that (i) BamA is essential for biogenesis of the TAA YadA, ( ...
Tópico(s): RNA and protein synthesis mechanisms
2010 - Wiley | Molecular Microbiology
John Huxley, Victoria McKee, Mark Skipworth Consumer Affairs Correspondent, Frank Muir, Barbara Hall, Arthur Smith, Mike Graham, Peter Green, Paul Nelson, Richard Ellis, Helen Davidson, Jon Swain, Kenneth Burgin, Rob Hughes, John Peter, Mark Reason, Norman MacRae, Tony Francis, Susan d'Arcy, Elizabeth McFadzean, B J R King, Rachel Billington, Graham Rose, Diana Wright Personal Finance Editor, Sally Payne, David Smith, Steve Clarke, William Green, Jim Poyser, Michael Thrasher, John Rowland, Mark Hosenball, Joy Weston, R. Buchanan-Dunlop, R Logue, Patrick Stoddart, Susan Marling, Robert Sandall, Iain Johnstone, David Brierley, Irwin Stelzer, Jani Allan, Ivan Fallon, Adam Hopkins, Deryk Brown, David Dougill, Sir David Napley, Dymphna Byrne, Peter Reading, Cliff Temple, Bryan Burrough, Jane Bird, Peter Wood, Steve Clarke Media Correspondent, Scott Smedley Budapest, George Armstrong, Egon Ronay, Hugh Canning, Margarette Driscoll, Colin Dryden, Stuart Sexton, Michael De Larrabeiti, Susan Crosland, David Cairns, John Melmoth, Julia Neuberger, D Render, Robert Harris, Caroline Lees, George Perry, John Cassidy, Sue Mott, Robin Marlar, Rose Shepherd, Paul Golding, Meryl Gordon, Lesley Abdela, Bernard Cafferty, Jon Freeman, James Poyser, Stuart Wavell, Doris Saatchi, Elisabeth Ayrton, Paul Donovan, Janette Marshall, Duncan Campbell, Peter Lewis, Artyom Zaitsev, Amy E Morton, Jeff Randall, Jonathan Sale, Fran Abrams, Chris Stephen Tomis, Charles Dance, Tim Rayment, Roy Cannon, Clare Boylan, W Hawkins, Jeremy Isaacs, Joan Forman, John Stalker, Rufus Olins, Tavleen Singh, David Lawrenson, Patricia Madden, G Blake, Stephen Ashworth, Brian Walden, Richard Hough, Gareth David, Brian Reading, Stephen Hough, Jane Garner, Peter Martin, Christopher Ward, Gareth Huw Davies, Malcolm Winton, Patrick Taylor-Martin, James Wilson, Richard Cook, Craig Brown, Diana Wright, Andrew Lorenz Industrial Editor, Chris Horrie, Stephen Jones, Stephen Thorpe, Desmond Shawe-Taylor, Brian Deer, Harry Mullan, Chris Campling, Richard Palmer, Digby Anderson, Jonathan Theobald, Roy Coombs, Martin Searby, Jeremy Lewis, Miranda Seymour, Ian Birrell, Patrick Rowley, Dr Owen Gallagher, Dr Sheridan Gilley Dept of Theology, Professor Robert Winston, Chris Jones, Andrew Hogg, Brenda McGilvray, Edward Campbell, Norman Stone, Clive Everton, Richard Woods, Peter Johnson, Angus Waycott, Frances Rafferty, Elisabeth Winkler, Marion Hume, Louise Branson Bama, Maurice Chittenden, Iola Smith, Amin Rajan, Ruby Millington, Aileen Ballantyne Medical Correspondent, Brian MacArthur, Rebecca Fowler, Michael Jones Political Editor, Andrew Yates, Edmund Nicholas, Geoffrey Hobbs, John Helyar, D J Taylor, Mihir Bose, Philip Beresford, Susannah Herbert, Godfrey Smith, Paul Barker, Brian Glanville, Alan Myers, Carmel McQuaid, Norman Macrae, John Carey, Caroline baker, Godfrey Golzen, Tony Moss, Ann Dally, Jim Fish Zagreb, Sarah Morris, Andrew Lorenz, John Furbisher, Anne Applebaum Lvov, David Wickers, Colin Rallings, Valerie Grove, Chris Lightbown, Jeff Randall City Editor, Hugh Pearman, Marina Vaizey, Margaret Park, Angela Long Innovation Editor, Frank Field, David Smith Economics Editor, Tony Hetherington, Garbo, Roy Foster, David Waterstone, Walter Ellis, Joanna Simon, Boris Schapiro,
Contents One in three children thinks sun goes around earth By our Political Staff: Tory right repudiates Tebbit Cricket smear could be fatal blow to ...
1990 - Gale Group | Sunday Times HA GDA
BamA interacts with the BamBCDE lipoproteins, and together they constitute the essential β-barrel assembly machine (BAM) ... biogenesis defect. Without BamB and BamE, wild-type BamA levels are significantly reduced, and the folding of the BamA β-barrel, as assessed by the heat-modifiability ... acid substitutions in the β-barrel domain of BamA improve both bacterial growth and OMP biogenesis in a bamB bamE mutant and restore BamA levels close to the BamB(+) BamE(+) level. The substitutions alter BamA β-barrel folding, and folding in the mutants ...
Tópico(s): Enzyme Structure and Function
2011 - American Society for Microbiology | Journal of Bacteriology
Lloyd, Ernest Newman, Frederick Grundy, Mr. Bonar, A. B., A. Podmore, E. N., G. G. Kekewich, Gen. Hertzog, M. W. W., G. J., Mrs. Herbert Vivian, Russell Stanard, Frank Rutter, Yarborugh, Canon Carnegie, Charles D. Law, W. Wright Hardwicke M. D., Robert Lorenz, Edmund Gosse, Sydney W. Carroll, Helene Gingold, Frederic Wm., R. J. Barrett (Managing Editor of "The Finanical Times."), G. S. Street, X., H. M. W., Mayfair, H. C. Minchin, Roland Atkinson, T. P. O'Connor M. P., Mr. Balfour's, L. W., D. R. G., L. Van Vliet, Crawfurd Price, H. L. R., Vi-Bama, Mr. Robson Scott's, Chas. R. Johns, Hannen Swaffer, Senex, Walter R. Williams, Gerald Biss, W. A. Propert, A. Brodie Fraser, Henry D'a. Blumberg, R. J. Barrett Financial Editor, A. M. H.,
State Express Multiple Display Advertising Items Goochs Ltd. Avon Golf Balls Western Australia 6% Inscribed Stock, 1930-1940 Barclays Bank Limited Swiss ...
1921 - Gale Group | Sunday Times HA GDA
Yanan Li, Abigail W. Porter, Adam C. Mumford, Xin Zhao, L. Y. Young,
To characterize the microbial community structure and bamA gene diversity involved in anaerobic degradation of toluene and benzoate under denitrifying conditions.Nitrate-reducing enrichment cultures were established on either toluene, benzoate ... by 16S rRNA gene-based PCR-DGGE analysis. bamA gene diversity was analysed using DGGE and cloning methods. The results showed that bamA gene related to bamA of Thauera chlorobenzoica was dominant in toluene and ... was obtained in benzoate cultures. Low diversity of bamA gene was observed, and some similarities of bamA ...
Tópico(s): Genomics and Phylogenetic Studies
2011 - Oxford University Press | Journal of Applied Microbiology
Shang‐Ling Pan, Xiao-Qiu Luo, Zeping Lu, Shaohua Lu, Huan Luo, Chengwu Liu, Caiyou Hu, Ming Yang, Lili Du, Zhen Song, Guo‐Fang Pang, Huayu Wu, Jinbo Huang, Jun-Hua Peng, Rui‐Xing Yin,
... longevity in Zhuang long-lived families residing in Bama area, a famous home of longevity in Guangxi, ... by PCR-restriction fragment length polymorphism in 391 Bama Zhuang long-lived families (BLF, n = 1467, age ... 43 years) and four control groups recruited from Bama and out-of-Bama area with or without a familial history of exceptional longevity: Bama non-long-lived families (BNLF, n = 586, age 44.81 ± 26.83 years), Bama non-Zhuang long-lived families (BNZLF, n = 444, ... of dyslipidemia were significantly higher in the three Bama families as compared to the two non-Bama ...
Tópico(s): Cholesterol and Lipid Metabolism
2012 - BioMed Central | Lipids in Health and Disease
P.J. Workman, Kristina Heide, Nicolas Giuliano, Nanhee Lee, James W. Mar, Phu Vuong, Drew Bennion, Rajeev Misra,
ABSTRACT The BamA protein of Escherichia coli plays a central role in the assembly of β-barrel outer membrane proteins (OMPs). The C-terminal domain of BamA folds into an integral outer membrane β-barrel, ... OMP reception and assembly. We show here that BamA misfolding, caused by the deletion of the R44 ... soluble POTRA domain. Besides misfolding, the expression of BamA ΔR44 from a low-copy-number plasmid confers ... hypersensitivity phenotype. A spontaneous drug-resistant revertant of BamA ΔR44 was found to carry an A18S substitution ...
Tópico(s): Escherichia coli research studies
2012 - American Society for Microbiology | Journal of Bacteriology
Margaret Leonard-Rivera, Rajeev Misra,
... negative bacteria, chloroplasts, and mitochondria. In Escherichia coli, BamA, a member of the Omp85 family, folds into ... Although the high-resolution structures of only the BamA POTRA domain of E. coli are available, the ... secretion system in Bordetella pertussis, suggests that the BamA β-barrel likely folds into a 16-stranded ... presumably folds back into the lumen of the BamA β-barrel like the FhaC counterpart, play an important role in OMP and/or BamA biogenesis. The conserved (641)RGF(643) residues of ...
Tópico(s): Protein Structure and Dynamics
2012 - American Society for Microbiology | Journal of Bacteriology
Paul A. Merguerian, Pramod Reddy, Diego Barrieras, Gregory J. Wilson, Kimberly A. Woodhouse, Darius Bägli, Gordon A. McLorie, Antoine E. Khoury,
... bladder substitution with porcine bladder acellular matrix allograft (BAMA) in a large animal model. Materials and methods ... and enzymatic extractions to render them acellular. The BAMA produced had the surgical handling and suture‐retaining properties of normal bladder tissue. Six pigs had BAMA segments implanted under general anaesthesia, through a low ... partial cystectomy. The defect was repaired with a BAMA patch (mean size 43.88 cm 2 , range ... and the bladders explanted. The native bladder and BAMA patch were analysed morphometrically to evaluate cellular re‐ ...
Tópico(s): Electrospun Nanofibers in Biomedical Applications
2000 - Wiley | BJU International
Katarina Bartoš Jansen, Susan L. Baker, Marcelo C. Sousa,
... protein complex consisting of the β-barrel OMP BamA and lipoproteins BamB, -C, -D, and -E. High ... determined. However, the overall complex architecture remains elusive. BamA is the central component of BAM and consists ... between BamB and a POTRA3–5 fragment of BamA. Extended loops 13 and 17 protruding from one ... the face of the POTRA3 β-sheet in BamA. The interface is stabilized by several hydrophobic contacts, ... hydrogen bonds, and a cation-π interaction between BamA Tyr-255 and BamB Arg-195. Disruption of ...
Tópico(s): Titanium Alloys Microstructure and Properties
2014 - Elsevier BV | Journal of Biological Chemistry
Rajeev Misra, Ryan Stikeleather, Rebecca Gabriele,
... belongs to the Omp85 superfamily. In Escherichia coli, BamA is the core β-barrel OMP and, together ... of BamD, an essential lipoprotein, and BamB in BamA biogenesis. Depletion of BamD caused impairment in BamA biogenesis and cessation of cell growth. These defects ... substitutions mapping within the β-barrel domain of BamA. However, in the absence of BamB, the positive effects of the β-barrel substitutions on BamA biogenesis under BamD depletion conditions were nullified. By employing a BamA protein bearing one such substitution, F474L, it was ...
Tópico(s): Protein Structure and Dynamics
2014 - Elsevier BV | Journal of Molecular Biology
Elena B. Volokhina, Jan Grijpstra, Frank Beckers, Erika Lindh, Viviane Robert, Jan Tommassen, Martine P. Bos,
The BamA protein is the key component of the Bam complex, the assembly machinery for outer membrane proteins (OMP) in gram-negative bacteria. We previously demonstrated that BamA recognizes its OMP substrates in a species-specific ... specificity in vivo by testing the functioning of BamA homologs of the proteobacteria Neisseria meningitidis, Neisseria gonorrhoeae, ... and in N. meningitidis. We found that no BamA functioned in another species than the authentic one, except for N. gonorrhoeae BamA, which fully complemented a N. meningitidis bamA mutant. ...
Tópico(s): Genomics and Phylogenetic Studies
2013 - Public Library of Science | PLoS ONE
Douglas F. Browning, Vassiliy N. Bavro, Jessica Mason, Yanina R. Sevastsyanovich, Amanda E. Rossiter, Mark Jeeves, Timothy J. Wells, Timothy J. Knowles, Adam F. Cunningham, James W. Donald, Tracy Palmer, Michael Overduin, Ian R. Henderson,
... molecular machine, the central component of which is BamA. Orthologues of BamA are found in all Gram-negative bacteria, chloroplasts ... outer membrane proteins (OMPs) into the outer membrane. BamA binds unfolded β-barrel precursors via the five ... at its N-terminus. The C-terminus of BamA folds into a β-barrel domain, which tethers BamA to the outer membrane and is involved in OMP insertion. BamA orthologues are found in all Gram-negative bacteria ... species-specificity by examining whether chimeric Escherichia coli BamA fusion proteins, carrying either the β-barrel or ...
Tópico(s): Genomics and Phylogenetic Studies
2015 - Wiley | Molecular Microbiology
Leonor Morgado, Kornelius Zeth, Björn M. Burmann, Timm Maier, Sebastian Hiller,
The insertase BamA is the central protein of the Bam complex responsible for outer membrane protein biogenesis in Gram-negative bacteria. BamA features a 16-stranded transmembrane β-barrel and ... weight of 88 kDa. Whereas the structure of BamA has recently been determined by X-ray crystallography, ... Here, we report solution NMR studies of different BamA constructs in three different membrane mimetic systems: LDAO ... and the detergent:protein ratio. The barrel of BamA is folded in micelles, bicelles and nanodiscs, but ...
Tópico(s): Lipid Membrane Structure and Behavior
2015 - Springer Science+Business Media | Journal of Biomolecular NMR
Pamela A. Doerner, Marcelo C. Sousa,
BamA is an essential component of the β-barrel assembly machine (BAM) that is responsible for insertion ... outer membrane proteins (OMPs) in Gram-negative bacteria. BamA is an OMP itself, and its β-barrel ... molecular mechanism remains poorly understood. Crystal structures of BamA and complementary molecular dynamics simulations have shown that ... has led to mechanistic models in which the BamA barrel seam functions as a lateral gate that ... such that a nascent barrel can bud from BamA. Consistent with this model, disulfide locking of the ...
Tópico(s): Protein Structure and Dynamics
2017 - American Chemical Society | Biochemistry
Bob Schiffrin, Antonio N. Calabrese, A.J. Higgins, Julia R. Humes, Alison E. Ashcroft, Antreas C. Kalli, David J. Brockwell, Sheena E. Radford,
... Escherichia coli periplasmic chaperones Skp and SurA, and BamA, the central subunit of the BAM complex, on ... and molecular dynamics simulations. We show that prefolded BamA promotes the release of tOmpA from Skp despite ... tOmpA complex. This activity is located in the BamA β-barrel domain, but is greater when full-length BamA is present, indicating that both the β-barrel ... chain length in synthetic liposomes we show that BamA has a greater catalytic effect on tOmpA folding ... membrane thinning/disorder by the transmembrane domain of BamA is greatest in thicker bilayers. Finally, we demonstrate ...
Tópico(s): Protein Structure and Dynamics
2017 - Elsevier BV | Journal of Molecular Biology
Anne L. McCabe, Dante P. Ricci, Modupe O. Adetunji, Thomas J. Silhavy,
... five-member complex composed of β-barrel OMP BamA and four associated lipoproteins, BamB, BamC, BamD, and ... into the outer membrane. The two essential proteins BamA and BamD interact to stabilize two subcomplexes, BamAB ... genetic and structural evidence suggests that interactions between BamA and BamD occur via an electrostatic interaction between ... conserved aspartate residue in a periplasmic domain of BamA and a conserved arginine in BamD. In this ... we characterize charge-change mutations at these key BamA and BamD residues and nearby charged residues in ...
Tópico(s): Enzyme Structure and Function
2017 - American Society for Microbiology | Journal of Bacteriology
Hongyong Zhang, Jiaojiao Huang, Zechen Li, Guosong Qin, Nan Zhang, Tang Hai, Qianlong Hong, Qiantao Zheng, Ying Zhang, Ruigao Song, Jing Yao, Chunwei Cao, Jianguo Zhao, Qi Zhou,
... fibroblast cells (PEFs; Fig EV1A). PEFs derived from Bama miniature pigs were labeled with either red fluorescence ... Complementation of cloned host embryos, derived from the Bama RFP-labeled PEFs, with injection of donor blastomeres, derived from Bama GFP-labeled PEFs. Scale bar, 100 μm. D. ... negative control with no genomic DNA loaded. Mut, Bama MITFL247S/L247S blastocyst DNA loaded. WT, Bama wild-type blastocyst DNA loaded. B1-B10, collected ... PCR amplification of MITF in the blastocysts. Mut, Bama MITFL247S/L247S blastocyst DNA loaded. WT1-WT2, DNA ...
Tópico(s): Ocular Disorders and Treatments
2018 - Springer Nature | EMBO Molecular Medicine
Muralidhar Tata, Anna Konovalova,
... to enhance the kinetics of the highly conserved BamA/BamD core. Here we report that BamE plays ... biochemical cross-linking suggest that RcsF accumulates on BamA when BamA cannot engage with BamD because of its limited ... is to promote proper coordination of RcsF-bound BamA with BamD to complete OMP assembly around RcsF. ... the absence of BamE, RcsF is stalled on BamA, thus blocking its function, and we identify the ... machinery, the Bam complex, consists of five components, BamA to -E, among which BamA and BamD are ...
Tópico(s): Cellular transport and secretion
2019 - American Society for Microbiology | mBio
Elizabeth M. Hart, Thomas J. Silhavy,
... contains two essential proteins, the β-barrel OMP BamA and a lipoprotein BamD, whereas the auxiliary lipoproteins ... individually nonessential. Here, we identify and characterize three bamA mutations, the E-to-K change at position 470 ( bamA E470K ), the A-to-P change at position 496 ( bamA A496P ), and the A-to-S change at position 499 ( bamA A499S ), that suppress the otherwise lethal Δ bamD , ... present to allow BamD to coordinate productively with BamA. Besides BamA regulation, BamD shares an additional essential ...
Tópico(s): Enzyme Structure and Function
2020 - American Society for Microbiology | Journal of Bacteriology
Jim E. Horne, David J. Brockwell, Sheena E. Radford,
... that only two are essential: the 16-stranded BamA and 26-stranded LptD (36Ruiz N. Kahne D. ... more remarkable considering that LptD itself relies on BamA for its assembly (37Lee J. Xue M. Wzorek ... Full Text PDF PubMed Scopus (46) Google Scholar). BamA (part of the β-barrel assembly machinery, BAM) ... distance between bacteria and eukaryotes, a homologue of BamA, Sam50, is retained in all mitochondria (70Paschen S. ... PDF PubMed Scopus (113) Google Scholar). Although only BamA and LptD are essential in E. coli under ...
Tópico(s): Antibiotic Resistance in Bacteria
2020 - Elsevier BV | Journal of Biological Chemistry
Mary R. Ferguson, Kristina N. Delgado, Shannon McBride, Isabel C. Orbe, Carson J. La Vake, Melissa J. Caimano, Qiana Mendez, Trevor F. Moraes, Anthony B. Schryvers, M. Anthony Moody, Justin D. Radolf, Michael P. Weiner, Kelly L. Hawley,
... s repertoire of outer membrane proteins (OMPs) includes BamA (β-barrel assembly machinery subunit A/TP0326), a ... and five periplasmic POTRA (polypeptide transport-associated) domains. BamA ECL4 antisera promotes internalization of Tp by rabbit peritoneal macrophages. Methods Three overlapping BamA ECL4 peptides and a two-stage, phage display ... antisera generated by immunizing mice and rabbits with BamA ECL4 displayed by a Pyrococcus furiosus thioredoxin scaffold ( Pf Trx BamA/ECL4 ). MAbs and antisera reactivities were evaluated by ...
Tópico(s): Virology and Viral Diseases
2023 - Frontiers Media | Frontiers in Immunology
Nakajohn Thewasano, Edward M. Germany, Yuki Maruno, Yukari Nakajima, Takuya Shiota,
... BAM complex is composed of two essential proteins (BamA and BamD) and three nonessential accessory proteins (BamB, ... BAM complex is composed of two essential proteins (BamA and BamD) and three nonessential accessory proteins (BamB, ... The central component of the BAM complex is BamA, a member of the Omp85 protein superfamily, which ... BAM complex is a heteropentameric complex composed of BamA, BamB, BamC, BamD, and BamE. The E. coli BamA, an OMP, contains five repeat polypeptide transport-associated ( ... like structure mainly through the POTRA domains of BamA and BamD. All accessory subunits interact with the ...
Tópico(s): Enzyme Structure and Function
2023 - Elsevier BV | Journal of Biological Chemistry
Yue Ding, Takuya Shiota, Anton P. Le Brun, Rhys A. Dunstan, Bo Wang, Hsien‐Yi Hsu, Trevor Lithgow, Hsin‐Hui Shen,
... OM).The core component of this multiprotein complex, BamA, is an evolutionarily conserved protein that carries five ... POTRA) domains that project from the outer membrane.BamA is essential for chaperoning the insertion of proteins ... this work, we have reconstituted a membrane containing BamA on a gold substrate and characterized structure of ... with dissipation and neutron reflectometry (NR).The purified BamA in n-dodecyl β-D-maltoside (DDM) was ... protein.The data shows the total reach of BamA was 120 Å and the embedding of membrane ...
Tópico(s): Bacterial Genetics and Biotechnology
2020 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Biomembranes
Douglas F. Browning, Sophie A. Matthews, Amanda E. Rossiter, Yanina R. Sevastsyanovich, Mark Jeeves, Jessica Mason, Timothy J. Wells, Catherine A. Wardius, Timothy J. Knowles, Adam F. Cunningham, Vassiliy N. Bavro, Michael Overduin, Ian R. Henderson,
... An essential component of this complex is the BamA protein, which binds unfolded β-barrel precursors via ... in its N-terminus. The C-terminus of BamA contains a β-barrel domain, which tethers BamA to the outer membrane and is also thought ... be involved in OMP insertion. Here we mutagenize BamA using linker scanning mutagenesis and demonstrate that all five POTRA domains are essential for BamA protein function in our experimental system. Furthermore, we generate a homology based model of the BamA β-barrel and test our model using insertion ...
Tópico(s): Genomics and Phylogenetic Studies
2013 - Public Library of Science | PLoS ONE
Nathan W. Rigel, Jon-David Schwalm, Dante P. Ricci, Thomas J. Silhavy,
... Bam complex is composed of the essential proteins BamA and BamD and three nonessential lipoproteins, BamB, BamC, ... BamE are important for stabilizing the interaction between BamA and BamD. We used comprehensive genetic analysis to clarify the interplay between BamA and the BamCDE subcomplex. Combining a ΔbamE allele ... BamE is related to the conformational state of BamA. In wild-type cells, BamA is sensitive to externally added proteinase K. Strikingly, ... ΔbamE mutant cells are treated with proteinase K, BamA is degraded beyond detection. Taken together, our findings ...
Tópico(s): Escherichia coli research studies
2011 - American Society for Microbiology | Journal of Bacteriology
Nathan W. Rigel, Dante P. Ricci, Thomas J. Silhavy,
... essential components of this complex are the OMP BamA [which contains a carboxyl-terminal β-barrel and ... proximal POTRA domain (P5) for stable interactions with BamA. We have previously reported that the BamA β-barrel assumes two different conformations. A method for conformation-specific labeling of BamA described here reveals that these conformers reflect the ... likely because it occupies the lumen of the BamA β-barrel in the latter case. A gain- ... Bam (Δ bamE ) both favor surface exposure of BamA L6, suggesting that BamD and BamE normally act ...
Tópico(s): Antibiotic Resistance in Bacteria
2013 - National Academy of Sciences | Proceedings of the National Academy of Sciences