Lingling Meng, Bufan Wu, Ling OuYang, Rou Peng, Yonglin Chen, Tang Zhi-juan, Min Zhang, Tanqing Xu, Yaling Wang, Sheng-Feng Lu, Xinyue Jing, Shuping Fu,
BackgroundImbalances between Bcl-2 and caspase-3 are significant evidence of apoptosis, which is considered an influential factor in rapidly occurring neuronal ... brain cell damage via either an increase in Bcl-2 or a reduction in caspase-3 exposure. The ... study aimed to investigate whether acupuncture could modulate Bcl-2 and caspase-3 expression through histone acetylation modifications, ... evaluate neurological damage following stroke. The expression of Bcl-2/caspase-3 mRNA was detected by real-time ...
Tópico(s): Barrier Structure and Function Studies
2024 - Elsevier BV | Heliyon
Guillem Pascual‐Pasto, Claudia Resa‐Pares, Helena Castillo‐Ecija, Rosario Aschero, Merce Baulenas‐Farres, Mònica Vilà-Ubach, Victor Burgueño, Leire Balaguer‐Lluna, Maria Cuadrado‐Vilanova, Nagore G. Olaciregui, Nuria Martinez-Velasco, Sara Pérez‐Jaume, Enrique de Álava, Òscar M. Tirado, Cinzia Lavarino, Jaume Mora, Ángel M. Carcaboso,
... antiapoptotic members of the B-cell lymphoma-2 (Bcl-2) family of proteins such as Bcl-2, Bcl-xL, Bcl-W and Mcl-1. Protein ( ... correlated positively, while immunoblotting and immunohistochemistry expression of Bcl-2 correlated negatively with the efficacy of nab-paclitaxel in Ewing sarcoma PDX. The negative correlation of Bcl-2 immunoblotting signal and activity was especially robust (r = ... correlation). Consequently, we evaluated pharmacological strategies to inhibit Bcl-2 during nab-paclitaxel treatment. We observed that the ...
Tópico(s): Neuroblastoma Research and Treatments
2023 - Elsevier BV | Biochemical Pharmacology
Hudie Wei, Haolan Wang, Genxin Wang, Lingzhi Qu, Longying Jiang, Shuyan Dai, Xiaojuan Chen, Ye Zhang, Zhuchu Chen, Youjun Li, Ming Guo, Yongheng Chen,
Abstract Mitochondrial apoptosis is strictly controlled by BCL-2 family proteins through a subtle network of protein interactions. The tumor suppressor protein p53 triggers transcription-independent apoptosis through direct interactions with BCL-2 family proteins, but the molecular mechanism is not ... DBD in complex with the anti-apoptotic protein BCL-2 at resolutions of 2.3–2.7 Å. ... penetrate directly into the BH3-binding pocket of BCL-2. Structure-based mutations at the interface impair the p53/BCL-2 interaction. Specifically, the binding sites for p53 and ...
Tópico(s): Hepatitis B Virus Studies
2023 - Nature Portfolio | Nature Communications
Andi Cahyadi, I Dewa Gede Ugrasena, Mia Ratwita Andarsini, Maria Christina Shanty Larasati, Aryati Aryati, Diah Kusuma Arumsari,
Background: Overexpression of the antiapoptotic protein Bcl-2 causes apoptosis to stop and conversely the increased expression of the proapoptotic protein Bax makes lymphoblasts easy to destroy. The Bax/Bcl-2 ratio plays a role in the balance of ... chemotherapy. We analyzed the relationship between the Bax/BCl-2 ratio and the outcome of induction phase chemotherapy ... on October 2020 to March 2021. Expression of Bcl-2, Bax, and Bax/Bcl-2 protein ratio was measured by the flow cytometry ... phase of chemotherapy went into remission. Bax and Bcl-2 expression before and after chemotherapy showed no difference ( ...
Tópico(s): Childhood Cancer Survivors' Quality of Life
2022 - West Asia Organization for Cancer Prevention | Asian Pacific Journal of Cancer Prevention
Chathura D. Suraweera, Suresh Banjara, Mark G. Hinds, Marc Kvansakul,
The B-cell lymphoma-2 (Bcl-2) family is a group of genes regulating intrinsic apoptosis, a process controlling events such as development, homeostasis and the innate and adaptive immune responses in metazoans. In higher organisms, Bcl-2 proteins coordinate intrinsic apoptosis through their regulation of ... appears to have originated in the basal metazoans. Bcl-2 genes predate the cnidarian-bilaterian split and have ... cnidarians but not ctenophores and some nematodes. The Bcl-2 family is composed of two groups of proteins, ...
Tópico(s): Venomous Animal Envenomation and Studies
2022 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Patricia M. A. Zehnle, Ying Wu, Henrike Pommerening, Miriam Erlacher,
•Antiapoptotic BCL-2 proteins are essential to maintain the hematopoietic system. •BCL-2 protein dependency varies between the different blood cell ... are novel anticancer drugs acting via inhibition of BCL-2 proteins. •Selective MCL-1 or BCL-XL inhibitors ... therapy, inducing apoptosis via inhibition of pro-survival BCL-2 proteins. Programmed cell death is fundamental for physiological ... setting; our knowledge of the more recently developed BCL-2 protein inhibitors specifically targeting MCL-1 or BCL- ... current knowledge of the function of specific antiapoptotic BCL-2 proteins in physiological hematopoiesis. BH3 mimetics constitute a ...
Tópico(s): Chronic Lymphocytic Leukemia Research
2022 - Elsevier BV | Experimental Hematology
Suli Lv, Xuefeng Zhao, Erlei Zhang, Yingying Yan, Xianyun Ma, Neng Li, Qingli Zou, Lidong Sun, Tanjing Song,
... Nakayama K.I. Inherent calcineurin inhibitor FKBP38 targets Bcl-2 to mitochondria and inhibits apoptosis.Nat. Cell Biol. ... H. Feng L. Yoon H.S. FKBP38 protects Bcl-2 from caspase-dependent degradation.J. Biol. Chem. 2010; ... Nakayama K.I. Inherent calcineurin inhibitor FKBP38 targets Bcl-2 to mitochondria and inhibits apoptosis.Nat. Cell Biol. ... J. Jarczowski F. Rahfeld J.U. et al.Bcl-2 regulator FKBP38 is activated by Ca2+/calmodulin.EMBO ... presenilins with FKBP38 promotes apoptosis by reducing mitochondrial Bcl-2.Hum. Mol. Genet. 2005; 14: 1889-1902Crossref PubMed ...
Tópico(s): Cancer-related gene regulation
2022 - Elsevier BV | Journal of Biological Chemistry
Lu Liu, Xianfeng Cheng, Hui Yang, Senlin Lian, Yuegen Jiang, Jin‐Hua Liang, X. Chen, Suo Mo, Yu Shi, Sishu Zhao, Jianyong Li, Runqiu Jiang, Dong‐Hua Yang, Yujie Wu,
... defects. The role of B-cell lymphoma-2 (BCL-2) in CLL has been explored; however, few studies ... Herein, we have investigated the regulatory role of BCL-2 in T cells in the CLL tumor microenvironment. Methods The expression of BCL-2 in T cells was evaluated using flow cytometry. The regulatory roles of BCL-2 were investigated using single-cell RNA sequencing (scRNA- ... and CD8 T cells. The clinical features of BCL-2 expression in T cells in CLL were also explored. Results We found a significant increase in BCL-2 expression in the T cells of CLL patients ( ...
Tópico(s): Lymphoma Diagnosis and Treatment
2022 - BioMed Central | Molecular Cancer
Émilie Narni-Mancinelli, Éric Vivier,
... the pro- and anti-apoptotic proteins of the BCL-2 family. Anti-apoptotic proteins (BCL-2, BCL-w, MCL-1, BFL-1, and BCL- ... in health and disease: the balancing act of BCL-2 family proteins.Nat. Rev. Mol. Cell Biol. 2019; ... Scopus (910) Google Scholar). A subset of BH3 (BCL-2 homology domain 3)-only proteins, including BIM and ... to identify cytotoxic molecules that directly act on BCL-2 protein family, such as the BH3 mimetics (Singh ... in health and disease: the balancing act of BCL-2 family proteins.Nat. Rev. Mol. Cell Biol. 2019; ...
Tópico(s): Virus-based gene therapy research
2022 - Cell Press | Cell
Manzar Alam, Sabeeha Ali, Taj Mohammad, Gulam Mustafa Hasan, Dharmendra Kumar Yadav, Md. Imtaiyaz Hassan,
... cell growth and survival. B cell lymphoma 2 (Bcl-2) is an anti-apoptotic molecule that plays a central role in apoptosis. Bcl-2 is the founding constituent of the Bcl-2 protein family of apoptosis controllers, the primary apoptosis regulators linked with cancer. Bcl-2 has been identified as being over-expressed in several cancers. Bcl-2 is induced by protein kinases and several signaling ... cancer development. Identifying the important function played by Bcl-2 in cancer progression and development, and treatment made ...
Tópico(s): Cancer-related Molecular Pathways
2021 - Multidisciplinary Digital Publishing Institute | International Journal of Molecular Sciences
Pratik Pal, Dinesh Thummuri, Dongwen Lv, Xingui Liu, Peiyi Zhang, Wanyi Hu, Saikat Kumar Poddar, Nan Hua, Sajid Khan, Yaxia Yuan, Xuan Zhang, Daohong Zhou, Guangrong Zheng,
BCL-XL and BCL-2 are important targets for cancer treatment. BCL-XL specific proteolysis-targeting chimeras (PROTACs) have been developed to circumvent the ... effects on cancer cells that are dependent on BCL-2 or both BCL-XL and BCL-2. Here we report a new series of BCL- ... XL degradation and in inhibiting but not degrading BCL-2, showing a hybrid dual-targeting mechanism of action ... is highly potent in killing BCL-XL dependent, BCL-2 dependent, and BCL-XL/BCL-2 dual-dependent cells in an E3 ligase (VHL)- ...
Tópico(s): Peptidase Inhibition and Analysis
2021 - American Chemical Society | Journal of Medicinal Chemistry
Ian de Ridder, Martijn Kerkhofs, P. V. Santhini, Wim Dehaen, Geert Bultynck,
The Bcl-2-family proteins have long been known for their role as key regulators of apoptosis. Overexpression of various ... associated with oncogenesis. Its founding member, anti-apoptotic Bcl-2 regulates cell death at different levels, whereby Bcl-2 emerged as a major drug target to eradicate ... This resulted in the development of venetoclax, a Bcl-2 antagonist that acts as a BH3 mimetic. Venetoclax ... leukemia patients. Here, we discuss the role of Bcl-2 as a decision-maker in cell death with ... cancer therapeutics that target the BH4 domain of Bcl-2, thereby interfering with non-canonical functions of Bcl- ...
Tópico(s): Cancer-related Molecular Pathways
2021 - Elsevier BV | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Xing Du, Jingjing Xiao, Xufeng Fu, Bo Xu, Hang Han, Yin Wang, Xiuying Pei,
B cell lymphoma 2 (Bcl-2) is an important antiapoptotic gene that plays a dual role in the maintenance of the dynamic balance between the survival ... death of cancer cells. In our previous study, Bcl-2 was shown to delay the G0/G1 to ... the detailed molecular mechanisms or pathways by which Bcl-2 regulates the cell cycle remain unknown. Here, we compared the effects of Bcl-2 overexpression with an empty vector control in the ... the effects using proteomic analysis. The effect of Bcl-2 on cell cycle regulation was detected by monitoring ...
Tópico(s): Cancer, Hypoxia, and Metabolism
2021 - Springer Science+Business Media | Journal of Zhejiang University SCIENCE B
Martijn Kerkhofs, Rita La Rovere, Kirsten Welkenhuysen, Ann Janssens, Peter Vandenberghe, Muniswamy Madesh, Jan B. Parys, Geert Bultynck,
Anti-apoptotic Bcl-2 critically controls cell death by neutralizing pro-apoptotic Bcl-2-family members at the mitochondria. Bcl-2 proteins also act at the endoplasmic reticulum, the ... channels are targeted by the BH4 domain of Bcl-2. Some cancer types rely on the IP3R-Bcl-2 interaction for survival. We previously developed a cell-permeable, BH4-domain-targeting peptide that can abrogate Bcl-2′s inhibitory action on IP3Rs, named Bcl-2 IP3 receptor disrupter-2 (BIRD-2). This peptide kills several Bcl-2-dependent cancer cell types, including diffuse large B- ...
Tópico(s): Cancer-related Molecular Pathways
2021 - Elsevier BV | Cell Calcium
Shuangfen Tao, Jian Gu, Qing Wang, Leizhen Zheng,
Abstract Background Anti-apoptotic protein Bcl-2 plays a substantial role in the carcinogenesis, whereas the regulation for Bcl-2 in gastric carcinoma (GC) is poorly understood. Specifically, ... of microRNA (miR)-383 in the control of Bcl-2 has not been shown in GC and thus ... We investigated the levels of miR-383 and Bcl-2 in 50 GC specimens, and compared them with ... assay were applied for analyzing the relationship between Bcl-2 and miR-383. An CCK assay was used ... miR-383 were lower while the levels of Bcl-2 levels were higher in GC specimens, compared to ...
Tópico(s): RNA Research and Splicing
2021 - BioMed Central | BMC Cancer
Fangbing Liu, Qiushi Zhao, Yongwei Su, Jing Lv, Yuqing Gai, Shuang Liu, Hai Lin, Yue Wang, Guan Wang,
... intrinsic apoptosis.•Co-inhibition of Mcl-1 and Bcl-2 has an effect on acquired AraC-resistant cells. ... remains a clinical challenge. Venetoclax is an effective Bcl-2 selective inhibitor approved by the U.S. Food ... facts, the combined inhibition of Mcl-1 and Bcl-2 shows promise against AML cells, including relapse/refractory ... remains a clinical challenge. Venetoclax is an effective Bcl-2 selective inhibitor approved by the U.S. Food ... facts, the combined inhibition of Mcl-1 and Bcl-2 shows promise against AML cells, including relapse/refractory ...
Tópico(s): RNA Interference and Gene Delivery
2021 - Elsevier BV | Experimental Hematology
Qiang Su, Yuli Xu, Ruping Cai, Rixin Dai, Xiheng Yang, Yang Liu, Binghui Kong,
... the mitochondria-dependent apoptotic pathway and increasing the Bcl-2/Bax ratio. miR-146a overexpression also blocked mitochondrial ... the mitochondria-dependent apoptotic pathway and increasing the Bcl-2/Bax ratio. miR-146a overexpression also blocked mitochondrial ... apoptotic pathway and cardiomyocyte survival. The anti-apoptotic Bcl-2 protein family and the pro-apoptotic Bax protein ... the initiation of mitochondrial apoptosis. Lower expression of Bcl-2 and higher expression of Bax were found in ... with the respective levels in untreated cells. The Bcl-2/Bax ratio, which determines the fate of cell ...
Tópico(s): Circular RNAs in diseases
2021 - Cell Press | Molecular Therapy — Nucleic Acids
Mario Tiribelli, Angela Michelutti, Margherita Cavallin, Sara Di Giusto, Erica Simeone, Renato Fanin, Daniela Damiani,
BCL-2 overexpression has been associated with resistance to chemotherapy and reduced survival in acute myeloid leukemia (AML), ... agents (HMAs) (n = 62), evaluating the role of BCL-2 expression on complete remission (CR) and overall survival (OS). BCL-2 was expressed in 81 patients (72%), more frequently ... in 34.5% cases, without differences according to BCL-2 expression or induction therapy. In the whole population 1-year OS was 39%, similar in BCL-2+ and BCL-2- cases. In BCL-2 positive patients OS was superior with HMAs (56% ...
Tópico(s): Histone Deacetylase Inhibitors Research
2021 - Multidisciplinary Digital Publishing Institute | Journal of Clinical Medicine
C. Ameh-Mensah, Babatunde Duduyemi, Kweku Bedu-Addo, E. Atta Manu, Francis Opoku, Nicholas Titiloye,
... tumor growth, and regression during treatment therapies. Antiapoptotic bcl-2 (gene) and p53 are known to play roles ... our study is to determine the association of bcl-2 (protein) with p53 and Ki-67 in 203 ... selected for tissue microarray (TMA) construction, and IHC (bcl-2 (protein), Ki-67, p53, cyclin D, pan cytokeratins ... PR, and HER2/neu) was done. Expressions of bcl-2 (protein), p53, and Ki-67 were related to ... and TNBC (54.3%) constituted the molecular classes. bcl-2 expression was found in 38% of the cases. ...
Tópico(s): Cancer Cells and Metastasis
2021 - Hindawi Publishing Corporation | Journal of Oncology
Anna Geueke, Giada Mantellato, Florian Kuester, Peter Schettina, Melanie Nelles, Jens M. Seeger, Hamid Kashkar, Catherin Niemann,
... August 2021Open Access Transparent process The anti-apoptotic Bcl-2 protein regulates hair follicle stem cell function Anna ... mammalian skin, elevated expression of the anti-apoptotic Bcl-2 protein has been reported in hair follicle (HF) ... show that systemic exposure of mice to the Bcl-2 antagonist ABT-199/venetoclax leads to the selective ... are upregulated in sbBSCs, explaining their addiction to Bcl-2 and the marked susceptibility to Bcl-2 antagonism. In line with these observations, conditional knockout ...
Tópico(s): Mesenchymal stem cell research
2021 - Springer Nature | EMBO Reports
Nesrine Helaly, Noha Esheba, Desouky E. Abou Ammo, Nahed Mohamed Elwan, Rasha A. Elkholy,
Determination of Bax/Bcl-2 ratio may be a good predictive tool to recognize chronic lymphocytic leukemia (CLL) patients’ outcome and prognosis to decide ... 100 patients with newly diagnosed CLL. Bax and Bcl-2 expression in peripheral blood were measured by flow-cytometry. The association of Bax/Bcl-2 ratio with CLL laboratory markers, Rai stage, overall ... was investigated. The sensitivity and specificity of Bax/Bcl-2 in predicting survival was evaluated. The best cut-off value of Bax/Bcl-2 ratio to predict the survival, detected by receiver ...
Tópico(s): Phagocytosis and Immune Regulation
2021 - Elsevier BV | Leukemia Research
Mengzhuang Gou, Shujuan Pan, Jinghui Tong, Yanfang Zhou, Jiarui Han, Ting Xie, Ting Yu, Wei Feng, Yanli Li, Song Chen, Yimin Cui, Baopeng Tian, Meng Tan, Zhiren Wang, Xingguang Luο, Chiang‐Shan R. Li, Ping Zhang, Junchao Huang, L. Elliot Hong, Yunlong Tan, Li Tian,
... schizophrenia, and one of its regulatory target genes BCL-2 (B-cell lymphoma 2) is suggested to associate ... that miR-181b-5p affects cognition by targeting BCL-2 mRNA and downregulating BCL-2 protein expression in schizophrenia patients. In this study, ... populations. Expression levels of miR-181b-5p and BCL-2 mRNA in peripheral whole blood were measured with quantitative real-time PCR (Q-PCR) and BCL-2 protein in plasma were measured with Enzyme Linked ... level was significantly upregulated (p = 0.001) whereas BCL-2 mRNA and BCL-2 protein levels were significantly ...
Tópico(s): Immune Cell Function and Interaction
2021 - Elsevier BV | Journal of Psychiatric Research
Arnaud LeTiran, Audrey Clapéron, James Davidson, Jérôme-Benoît Starck, Thierry Le Diguarher, Maïa Chanrion, Prakash Mistry, Youzhen Wang, Elodie Monceau, Fabienne Bernhardt, Francesca Rocchetti, Gaëlle Lysiak-Auvity, I‐Jen Chen, Zoe Daniels, Chris Pedder, Mandy Fallowfield, Jean‐Michel Henlin, Imre Fejes, János Tatai, Miklós Nyerges, Didier Durand, Marion Zarka, Sneha Sanghavi, Anne-Marie Girard, Marie Schoumacher, Laurence Kraus‐Berthier, Rick Newcombe, Ensar Halilovic, Sébastien Banquet, Alain Rupin, Heiko Maacke, James B. Murray, Erick J. Morris, Francesco Hofmann, Frédéric Colland, Olivier Geneste,
Abstract The B-cell Lymphoma 2 (BCL-2) gene family encodes pro-apoptotic and anti-apoptotic proteins that are key regulators of the apoptotic process. Overexpression of the pro-survival member BCL-2 is a well-established mechanism contributing to oncogenesis ... including lymphoma and leukemia. Venetoclax (Venclexta™), a selective BCL-2 inhibitor, is the first member of a new ... the identification of a novel potent and selective BCL-2 inhibitor named S65487/VOB560 that has a different binding mode on BCL-2 compared to Venetoclax. This inhibitor binds to the ...
Tópico(s): Cell death mechanisms and regulation
2021 - American Association for Cancer Research | Cancer Research
Milan Vu, Nick Kassouf, Rosemary Ofili, Torben Lund, Celia Bell, Sandra S. Appiah,
The overexpression of anti‑apoptotic Bcl‑2 in acute myeloid leukaemia (AML) may contribute to difficulties in eradicating these cells during chemotherapy. In the present study, doxorubicin (Dox) ... MOLM‑13 cells and to modulate autophagy through Bcl‑2 and Beclin 1 protein expression. Annexin V/propidium ... 937 monocytic cells. The protein expression levels of Bcl‑2 and Beclin 1 in untreated and treated cells ... protein levels in U‑937 monocytes. A novel Bcl‑2 15‑20 kDa (p15‑20‑Bcl‑2) isoform was found to be selectively expressed in ...
Tópico(s): Phagocytosis and Immune Regulation
2020 - Spandidos Publishing | International Journal of Oncology
Suresh Banjara, Chathura D. Suraweera, Mark G. Hinds, Marc Kvansakul,
... the interplay of the B-cell lymphoma 2 (Bcl-2) family and their membrane interactions. Bcl-2 proteins mediate a number of processes including development, ... underpins a host of diseases including cancer. The Bcl-2 family is characterized by the presence of conserved sequence motifs called Bcl-2 homology motifs, as well as a transmembrane region, ... the interaction sites and intracellular location mechanism, respectively. Bcl-2 proteins have been recognized in the earliest metazoans ... g., Hydra). A number of viruses have gained Bcl-2 homologs and subvert innate immunity and cellular apoptosis ...
Tópico(s): Aquaculture disease management and microbiota
2020 - Multidisciplinary Digital Publishing Institute | Biomolecules
Ni Li, Linwen Zhu, Hua Zhou, Dawei Zheng, Guodong Xu, Lebo Sun, Jianqing Gao, Guofeng Shao,
... for anti-apoptotic factor, B-cell lymphoma 2 (BCL-2) contributes to apoptosis in patients with rheumatic heart ... cell models. The binding of miR-1183 and Bcl-2 gene was confirmed by luciferase activity test. We also measured expression levels of BCL-2 in heart valve tissue from patients with RHD ... that miR-1183 directly targets the mRNA encoding BCL-2. It is found that miR-1183 binds directly to the 3'UTR of the BCL-2 mRNA and down-regulates the mRNA and protein levels of BCL-2. Overexpression of miR-1183 in RHD patients and ...
Tópico(s): Rheumatoid Arthritis Research and Therapies
2020 - Portland Press | Bioscience Reports
Ruby Dwivedi, Shaleen Chandra, Divya Mehrotra, Vineet Raj, Rahul Pandey,
Bcl-2 (B cell Lymphoma -2) family comprises of both anti-apoptotic and pro-apoptotic proteins whose altered ... this study is to assess the usefulness of Bcl-2 in distinguishing dysplastic or malignant epithelium from non- ... 5 normal tissue samples. The labeling index of Bcl-2 was analyzed in immunohistochemically stained sections. Different statistical ... used to analyze the data and to compare Bcl-2 expression qualitatively and quantitatively among all the groups.An increasing trend of Bcl-2 immunoexpression was observed from normal epithelium to non- ...
Tópico(s): Cell death mechanisms and regulation
2020 - Elsevier BV | Journal of Oral Biology and Craniofacial Research
Marta Di Martile, Valentina Farini, Francesca Maria Consonni, Daniela Trisciuoglio, Marianna Desideri, Elisabetta Valentini, Simona D’Aguanno, Maria Grazia Tupone, Simonetta Buglioni, Cristiana Ercolani, Enzo Gallo, Bruno Amadio, Irene Terrenato, Maria Laura Foddai, Antonio Sica, Donatella Del Bufalo,
... to therapy. Our previous studies have demonstrated that bcl-2 affects melanoma progression and regulates the tumor microenvironment. ... aim of this study was to evaluate whether bcl-2 expression in melanoma cells could influence tumor-promoting ... macrophages and melanoma cells expressing different levels of bcl-2 protein were used. ELISA, qRT-PCR and Western ... exposed to culture media from melanoma cells overexpressing bcl-2, while bcl-2 silencing in melanoma cells inhibited the M2 macrophage ... increased, in parallel with a greater expression of bcl-2 in tumor cells. Tumor-derived interleukin-1β has ...
Tópico(s): Cancer Immunotherapy and Biomarkers
2020 - BMJ | Journal for ImmunoTherapy of Cancer
Suresh Banjara, D Jaison, Mark G. Hinds, Marc Kvansakul,
... Proteins homologous to the B-cell lymphoma 2 (Bcl-2) family of proteins, the primary arbiters of mitochondrially ... mapped interactions between pro-survival and pro-apoptotic Bcl-2 proteins of H. vulgaris by affinity measurements between Hy-Bcl-2-4, the sole confirmed pro-survival Bcl-2 protein, with BH3 motif peptides of two Bcl-2 proteins from hydra that displayed pro-apoptotic activity, ... peptides from hydra encoded pro-apoptotic proteins, Hy-Bcl-2-4 also engaged BH3 motif peptides from multiple human pro-apoptotic Bcl-2 proteins. Reciprocally, human pro-survival Bcl-2 proteins ...
Tópico(s): Erythrocyte Function and Pathophysiology
2020 - Portland Press | Biochemical Journal
Roya Kolahdouz‐Mohammadi, Ali‐Akbar Delbandi, Sepideh Khodaverdi, Soheila Arefi, Tahereh Arablou, Farzad Shidfar,
... to examine resveratrol effects on gene expression of Bcl-2, Bax and Bcl-2/Bax ratio in endometrial stromal cells derived from ... without resveratrol (100 µM) and the levels of Bcl-2, Bax and Bcl-2/Bax gene expression ratio in the cells from ... by real-time PCR. Results: Resveratrol treatment increased Bcl-2 expression in CESCs at 24 and 48 h ... at 48 h (P<0.05). Regarding the Bcl-2/Bax gene expression ratio, resveratrol treatment increased Bcl-2/Bax gene expression ratio in CESCs and EuESCs ... this treatment had no significant differential effect on Bcl-2 and Bcl-2/Bax gene expression ratio between ...
Tópico(s): Endometrial and Cervical Cancer Treatments
2020 - Knowledge E | Iranian Journal of Public Health