A. M. J. J. Verweij-Van Vught, Ben R. Otto, F. Namavar, M. Sparrius, D. M. MacLaren,
Of the five Bacteriodes species of the 'fragilis group' only Bacteroides fragilis was able to grow in human plasma. Therefore the capacity of several iron sources to stimulate to growth of Bacteroides species under iron restricted conditions in vitro was tested. The iron chelator bipyridyl was used for the restriction of iron in the media. Ferrous sulphate, ferric ammonium sulphate and ferric citrate stimulated the growth of all five Bacteroides species tested to the same extent. B. fragilis, and ...
Tópico(s): Radioactive element chemistry and processing
1988 - Oxford University Press | FEMS Microbiology Letters
Henriëtte Levenga, Peter Donnelly, Nicole M. A. Blijlevens, Paul E. Verweij, Hebste Shirango, Ben de Pauw,
Tópico(s): Bacterial Identification and Susceptibility Testing
2003 - Springer Science+Business Media | Annals of Hematology
Cornelis L. Verweij, Carlie J.M. de Vries, Ben Distel, Anton Jan van Zonneveld, Ad Geurts van Kessel, Jan A. van Mourik, Hans Pannekoek,
Von Willebrand Factor (vWF) mRNA was identified in fractionated polyA+ RNA preparations isolated from cultured human endothelial cells. Micro-injection of specific polyA+ RNA fractions in Xenopus laevis oocytes provoked the synthesis of a vWF-like product which could be detected with an immunoradiometric assay relying on Sepharose-linked monoclonal anti-vWF IgG and different radiolabeled monoclonal anti-vWF IgGs. A vWF-mRNA-containing polyA+ RNA preparation served as substrate for a size-selected ...
Tópico(s): T-cell and B-cell Immunology
1985 - Oxford University Press | Nucleic Acids Research
A. M. J. J. Verweij-Van Vught, Ben J. Appelmelk, A. B. Johan Groeneveld, M. Sparrius, L. G. Thijs, D. M. MacLaren,
Tópico(s): Cardiac Ischemia and Reperfusion
1987 - Birkhäuser | Agents and Actions
R. B. M. Landewé, A. M. M. Miltenburg, M.J.A. Verdonk, C L Verweij, F. C. Breedveld, M. R. Daha, Ben A. C. Dijkmans,
SUMMARY Chloroquine (Chi) is an anti-rheumatic drug that is widely used in the treatment of rheumatoid arthritis (RA). It seems that T ceils are important in the pathogenesis of RA, but it is not known whether Chl acts via inhibition of T cell function. We here present evidence that Chl, just like cyclosporine A (CsA), inhibits Tcell proliferation as induced with immobilized αCD3 MoAb in a concentration-dependent manner, at least partly through interfering with the production of IL-2 protein and the ...
Tópico(s): Chemokine receptors and signaling
1995 - Oxford University Press | Clinical & Experimental Immunology
Ben R. Otto, Walter Verweij, M. Sparrius, A M Verweij-van Vught, Nord Ce, D. M. MacLaren,
Under conditions of iron starvation, Bacteroides fragilis expresses various iron-repressible outer membrane proteins (IROMPs). A 44-kDa protein appears to be one of the major outer membrane proteins (OMPs) in B. fragilis under iron stress and plays a role in heme uptake by this bacterium. To determine whether the 44-kDa IROMP of B. fragilis is expressed in vivo and whether this protein is immunogenic, we used Western immunoblotting to examine serum samples from patients with an infection caused by ...
Tópico(s): Diphtheria, Corynebacterium, and Tetanus
1991 - American Society for Microbiology | Infection and Immunity
S. Tetar, A. Bruynzeel, Lisa Verweij, Omar Bohoudi, Ben J. Slotman, T. Rosario, M. Palacios, Frank J. Lagerwaard,
Magnetic resonance-guided radiotherapy with daily plan adaptation for intermediate- and high-risk prostate cancer is time and labor intensive. Fifty adapted plans with 3 mm planning target volume (PTV)-margin were compared with non-adapted plans using 3 or 5 mm margins. Adequate (V95% ≥ 95%) prostate coverage was achieved in 49 fractions with 5 mm PTV without plan adaptation, however, coverage of the seminal vesicles (SV) was insufficient in 15 of 50 fractions. There was no insufficient coverage for ...
Tópico(s): Advanced MRI Techniques and Applications
2022 - Elsevier BV | Physics and Imaging in Radiation Oncology
Marjolijn C.A. Blans, Ben U. Ridwan, Jaco J. Verweij, M. Rozenberg-Arska, J. Verhoef,
Abstract We describe an outbreak of Cyclospora cayetanensis infection among Dutch participants at a scientific meeting in September 2001 in Bogor, Indonesia. Fifty percent of the investigated participants were positive for C. cayetanensis. To our knowledge, this outbreak is the first caused by C. cayetanensis among susceptible persons in a disease-endemic area.
Tópico(s): Amoebic Infections and Treatments
2005 - Centers for Disease Control and Prevention | Emerging infectious diseases
M. Kloppenburg, C. L. Verweij, A. M. M. Miltenburg, AJ Verhoeven, M. R. Daha, Ben A. C. Dijkmans, F. C. Breedveld,
SUMMARY Minocycline has been shown to have an anti-inflammatory effect in patients with rheumatoid arthritis (RA). Since there is evidence that RA is a T cell-mediated disease, we investigated the effect of minocycline on human T cell clones derived from the synovium of an RA patient. The T cells, when activated via the T cell receptor (TCR)/CD3 complex, were suppressed functionally by minocycline, resulting in a dose-dependent inhibition of T cell proliferation and reduction in production of lL-2. ...
Tópico(s): Immune Cell Function and Interaction
1995 - Oxford University Press | Clinical & Experimental Immunology
Hennie G. Raterman, Saskia Vosslamber, Sander de Ridder, Michael T. Nurmohamed, Willem F. Lems, Maarten Boers, Mark A. van de Wiel, Ben A. C. Dijkmans, Cornelis L. Verweij, Alexandre E. Voskuyl,
Abstract Introduction B cell depletion therapy is efficacious in rheumatoid arthritis (RA) patients failing on tumor necrosis factor (TNF) blocking agents. However, approximately 40% to 50% of rituximab (RTX) treated RA patients have a poor response. We investigated whether baseline gene expression levels can discriminate between clinical non-responders and responders to RTX. Methods In 14 consecutive RA patients starting on RTX (test cohort), gene expression profiling on whole peripheral blood RNA ...
Tópico(s): Rheumatoid Arthritis Research and Therapies
2012 - BioMed Central | Arthritis Research & Therapy
M. Kloppenburg, Ben A. C. Dijkmans, Cor L. Verweij, Ferdinand C. Breedveld,
The objective of this study was to analyze the anti-inflammatory effect of minocycline in rheumatoid arthritis. Serum samples of 65 RA patients who completed a 26-week randomized double-blind trial of minocycline (100 mg twice a day) versus placebo were studied. In this trial some clinical parameters and in particular the acute phase response decreased significantly in the minocycline-treated group. Serum levels of albumin and interleukin-6 (IL-6) were compared with CRP levels in order to study the ...
Tópico(s): Toxin Mechanisms and Immunotoxins
1996 - Elsevier BV | Immunopharmacology
M. Kloppenburg, Brigitta M. N. Brinkman, H.H. de Rooij-Dijk, A. M. M. Miltenburg, Mohamed R. Daha, F. C. Breedveld, Ben A. C. Dijkmans, C. L. Verweij,
Minocycline is a tetracycline derivative that has beneficial effects in noninfectious forms of arthritis and dermatitis. To investigate whether this effect may be attributed to interference with cytokine production, we studied the effect of minocycline on cytokine production by T cells and monocytes. Minocycline exerted an inhibitory effect on tumor necrosis factor alpha (TNF-alpha) and gamma interferon production by stimulated T cells, whereas the production of interleukin 6 (IL-6) remained unaffected. ...
Tópico(s): Phytochemistry and biological activity of medicinal plants
1996 - American Society for Microbiology | Antimicrobial Agents and Chemotherapy
Ben J. Appelmelk, A. M. J. J. Verweij-Van Vught, D. M. MacLaren, L. G. Thijs,
Bovine serum albumin was complexed with the core antigens of either Escherichia coli J5 LPS, Salmonella minnesota R595 LPS or E. coli lipid A. These core-BSA complexes were used for solid-phase coating in ELISAs for anti-core antibodies. Antibodies, binding to various parts of the core region were easily quantified in a single experimental set-up, which was hitherto not possible. The ELISA has only 3 incubation steps and is not costly as only moderate amounts of the core antigens (i.e. μg per test) ...
Tópico(s): Protein purification and stability
1985 - Elsevier BV | Journal of Immunological Methods
Ben J. Appelmelk, A. M. J. J. Verweij-Van Vught, Janneke J. Maaskant, W. F. Schouten, L. G. Thijs, D. M. MacLaren,
Following immunization of mice with Salmonella minnesota strain R595, monoclonal antibodies to R595 lipopolysaccharide were prepared. These belonged to various (sub)classes: IgG2a, IgG2b, IgG3 and IgM. Ig2a clones were obtained only when acetone-killed bacteria were used for immunization. Long-course immunizations yielded more clones than short courses. The reactivity of the monoclonal antibodies towards a panel of S. minnesota rough strain lipopolysaccharides (Ra to Re chemotype) was determined. ...
Tópico(s): Bacteriophages and microbial interactions
1987 - Oxford University Press | FEMS Microbiology Letters
Ben J. Appelmelk, A. M. J. J. Verweij-Van Vught, Janneke J. Maaskant, W. F. Schouten, Abraham J. R. Jonge, L. G. Thijs, D. M. MacLaren,
SUMMARY Monoclonal antibodies to the lipopolysaccharide (LPS) core region were produced by immunising mice with Escherichia coli strain J5 (chemotype Rc). One of these bound to the deepest part of the core, i.e., Lipid A, and reacted with other heat-killed but not live gram-negative bacilli, including E. coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. Eight other monoclonal antibodies, binding to the terminal glucose residue of Rc LPS, reacted with live cells of E. coli strains only. Thus, ...
Tópico(s): Bacterial Genetics and Biotechnology
1988 - Microbiology Society | Journal of Medical Microbiology
Ben R. Otto, A. M. J. J. Verweij-Van Vught, J. van Doorn, D. M. MacLaren,
A virulent strain B. fragilis BE1 and an avirulent strain B. vulgatus BE20 were grown in a culture medium with and without the addition of a synthetic chelator (Bipyridyl) to induce iron limitation. Cells grew more slowly under iron stress, although the growth rate of the B. vulgatus strain was more affected under these conditions than the strain of B. fragilis. The outer membrane protein profile of these strains was studied in relation to the iron concentration in the growth medium by means of SDS- ...
Tópico(s): Genomics and Phylogenetic Studies
1988 - Elsevier BV | Microbial Pathogenesis
Ben R. Otto, A. M. J. J. Verweij-Van Vught, D. M. MacLaren,
The low concentration of free iron in body fluids creates bacteriostatic conditions for many microorganisms and is therefore an important defense factor of the body against invading bacteria. Pathogenic bacteria have developed several mechanisms for acquiring iron from the host. Siderophore-mediated iron uptake involves the synthesis of low molecular weight iron chelators called siderophores which compete with the host iron-binding glycoproteins lactoferrin (LF) and transferrin (TF) for iron. Other ...
Tópico(s): Bacteriophages and microbial interactions
1992 - Taylor & Francis | Critical Reviews in Microbiology
Ben R. Otto, A. M. J. J. Verweij-Van Vught, D. M. MacLaren,
Tópico(s): Blood disorders and treatments
1992 - Nature Portfolio | Nature
Ben R. Otto, M. Sparrius, A M Verweij-van Vught, D. M. MacLaren,
Under iron starvation, Bacteroides fragilis expresses various iron-regulated outer membrane proteins. In this study, a deferrated minimal medium was used in growth experiments, and the role of one of these iron-regulated outer membrane proteins (a 44-kDa protein) in an iron uptake mechanism which acquires iron from heme compounds was elucidated. When a specific 44-kDa protein antiserum was used in a medium with heme as the only iron source, growth inhibition was observed. These results demonstrate ...
Tópico(s): Microbial Community Ecology and Physiology
1990 - American Society for Microbiology | Infection and Immunity
Lisa G. M. van Baarsen, Carla A. Wijbrandts, François Rustenburg, Tineke Cantaert, Tineke CTM van der Pouw Kraan, Dominique Baeten, Ben A. C. Dijkmans, Paul P. Tak, Cornelis L. Verweij,
Cross-regulation between TNF and type I IFN has been postulated to play an important role in autoimmune diseases. Therefore, we determined the effect of TNF blockade in rheumatoid arthritis (RA) on the type I IFN response gene activity in relation to clinical response.Peripheral blood from 33 RA patients was collected in PAXgene tubes before and after the start of infliximab treatment. In a first group of 15 patients the baseline expression of type I IFN-regulated genes was determined using cDNA ...
Tópico(s): Cytokine Signaling Pathways and Interactions
2010 - BioMed Central | Arthritis Research & Therapy
Lisa G. M. van Baarsen, Carla A. Wijbrandts, Daniëlle M. Gerlag, François Rustenburg, Tineke C. T. M. van der Pouw Kraan, Ben A. C. Dijkmans, Paul P. Tak, C L Verweij,
Tópico(s): Systemic Lupus Erythematosus Research
2010 - Springer Nature | Genes and Immunity
Lisa G. M. van Baarsen, Wouter H Bos, François Rustenburg, Tineke C. T. M. van der Pouw Kraan, Gertjan Wolbink, Ben A. C. Dijkmans, Dirkjan van Schaardenburg, Cornelis L. Verweij,
Abstract Objective To identify molecular features associated with the development of rheumatoid arthritis (RA), to understand the pathophysiology of preclinical development of RA, and to assign predictive biomarkers. Methods The study group comprised 109 anti–citrullinated protein antibody (ACPA)– and/or rheumatoid factor–positive patients with arthralgia who did not have arthritis but were at risk of RA, and 25 patients with RA. The gene expression profiles of blood samples obtained from these patients ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2010 - Wiley | Arthritis & Rheumatism
Tineke C. T. M. van der Pouw Kraan, Lisa G. M. van Baarsen, Carla A. Wijbrandts, Alexandre E. Voskuyl, François Rustenburg, Josefien M. Baggen, Ben A. C. Dijkmans, Paul P. Tak, C L Verweij,
Rheumatoid arthritis (RA) is a heterogeneous disease with unknown etiology. Here we aimed to distinguish RA subtypes based on peripheral blood (PB) gene expression profiles in comparison with a pathogen-response transcriptional program. PB was obtained from 35 RA patients and 15 healthy individuals. For expression profiling we used DNA microarrays. A combined cluster analysis of RA and control samples together with samples from a viral infection model revealed that the gene expression profile of a ...
Tópico(s): Herpesvirus Infections and Treatments
2007 - Springer Nature | Genes and Immunity
Tineke C. T. M. van der Pouw Kraan, Carla A. Wijbrandts, Lisa G. M. van Baarsen, Alexandre E. Voskuyl, François Rustenburg, Josefien M. Baggen, Saleh Ibrahim, Mike Fero, Ben A. C. Dijkmans, P. P. Tak, C L Verweij,
Background: Rheumatoid arthritis (RA) is a heterogeneous disease with unknown cause. Aim: To identify peripheral blood (PB) gene expression profiles that may distinguish RA subtypes. Methods: Large-scale expression profiling by cDNA microarrays was performed on PB from 35 patients and 15 healthy individuals. Differential gene expression was analysed by significance analysis of microarrays (SAM), followed by gene ontology analysis of the significant genes. Gene set enrichment analysis was applied to identify pathways ...
Tópico(s): Rheumatoid Arthritis Research and Therapies
2007 - BMJ | Annals of the Rheumatic Diseases
Saskia Vosslamber, Hennie G. Raterman, Tineke C. T. M. van der Pouw Kraan, Marco W.J. Schreurs, B. Mary E. von Blomberg, Michael T. Nurmohamed, Willem F. Lems, Ben A. C. Dijkmans, Alexandre E. Voskuyl, Cornelis L. Verweij,
Objective Despite the fact that rituximab depletes B cells in all treated patients with RA, not all patients show a favourable clinical response. The goal of this study was to provide insight into pharmacological changes in peripheral blood that are associated with clinical response to rituximab. Methods Gene expression profiling was performed on peripheral blood RNA of 13 patients with RA (test group) using Illumina HumanHT beadchip microarrays. An independent group of nine patients was used for ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2011 - BMJ | Annals of the Rheumatic Diseases
N. M. Van der Meer, Ben J. Appelmelk, A M Verweij-van Vught, Wolfgang Nimmich, Paul Kosma, L. G. Thijs, J. de Graaff, D. M. MacLaren,
A monoclonal antibody (MAb) raised against Salmonella minnesota R595 and specific for alpha-3-deoxy-D-manno-octulosonic acid (alpha-Kdo) of the inner core was tested for binding to lipopolysaccharides (LPS) of Klebsiella pneumoniae. The MAb was tested in several assay systems (enzyme-linked immunosorbent assay, passive hemolysis, and inhibition of passive hemolysis) with a large panel (n = 23) of K. pneumoniae LPS representing all nine currently known O serotypes. MAb 20 showed reactivity with almost all ...
Tópico(s): Mycobacterium research and diagnosis
1994 - American Society for Microbiology | Infection and Immunity
Monserrat Garcı́a, Gerhard van Vliet, Mattijs G. J. ten Cate, Francisco Chávez, Ben Norder, Bart J. Kooi, Werner E. van Zyl, Henk Verweij, Dave H. A. Blank,
Abstract Solution impregnations, pulltrusion and film stacking are widely used methods to prepare thermoplastic composite materials. Extruders are used to melt the polymer and to incorporate fibers into the polymer in order to modify physical properties. In this article, the compounding of colloidal silica nanoparticles filled polyamide‐6 (PA‐6) is achieved using a twin‐screw extruder, which has a significant market share due to its low cost and easy maintenance. The experiments were performed at ...
Tópico(s): Polymer crystallization and properties
2004 - Wiley | Polymers for Advanced Technologies
Hennie G. Raterman, Saskia Vosslamber, Sander de Ridder, Tamrah de Jong, Michael T. Nurmohamed, Willem F. Lems, Maarten Boers, Mark van de Wiel, Ben A. C. Dijkmans, Cornelis L. Verweij, Alexandre E. Voskuyl,
Background and objectives B cell depletion therapy is efficacious in rheumatoid arthritis (RA) patients failing on tumour necrosis factor (TNF) blocking agents. However, approximately 40–50% of rituximab (RTX) treated RA patients have a poor response. The authors investigated whether baseline gene expression levels can discriminate between clinical non-responders and responders to RTX. Materials and methods In 14 consecutive RA patients starting with RTX (test cohort), gene expression profiling on whole peripheral ...
Tópico(s): Monoclonal and Polyclonal Antibodies Research
2012 - BMJ | Annals of the Rheumatic Diseases
Ben J. Appelmelk, Donghui Su, A. M. J. J. Verweij-Van Vught, B G Thijs, D. M. MacLaren,
The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). We prepared covalent conjugates of PMB and horseradish peroxidase (HRP) by periodation of HRP-linked oligosaccharides followed by direct condensation with PMB. In addition we prepared monoclonal antibodies (Mabs) to PMB. The PMB-HRP conjugates and anti-PMB Mabs were used to study in ELISA the binding of PMB to LPS from Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. In addition, PMB- ...
Tópico(s): Antimicrobial Peptides and Activities
1992 - Elsevier BV | Analytical Biochemistry
Radboud J. E. M. Dolhain, Patricia Kuiper, C. L. Verweij, J. M. A. FENDERS, F C Breedveld, Ben A. C. Dijkmans, A. M. M. Miltenburg,
T-lymphocytes are involved in the inflammatory response that occurs in affected joints of patients with rheumatoid arthritis (RA). Some second-line disease modifying anti-rheumatic drugs used in the treatment of patients with RA are known to block T-cell activation. The present study assessed whether tenidap, an investigational anti-rheumatic drug, affects in vitro T-cell responses such as proliferation and cytokine production. It was found that tenidap, in contrast to several nonsteroidal anti-inflammatory ...
Tópico(s): Cytokine Signaling Pathways and Interactions
1995 - Wiley | Scandinavian Journal of Immunology